Impact of high-intensity pulsed electric fields on carotenoids profile of tomato juice made of moderate-intensity pulsed electric field-treated tomatoes

2013 ◽  
Vol 141 (3) ◽  
pp. 3131-3138 ◽  
Author(s):  
Anna Vallverdú-Queralt ◽  
Isabel Odriozola-Serrano ◽  
Gemma Oms-Oliu ◽  
Rosa M Lamuela-Raventós ◽  
Pedro Elez-Martínez ◽  
...  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
High Intensity ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Tomato Juice ◽  
Moderate Intensity

Pulsed Electric Fields Versus Thermal Treatment: Equivalent Processes To Obtain Equally Acceptable Citrus Juices

2006 ◽  
Vol 69 (8) ◽  
pp. 2016-2018 ◽  
Author(s):  
E. SENTANDREU ◽  
L. CARBONELL ◽  
D. RODRIGO ◽  
J. V. CARBONELL
Keyword(s):  
Thermal Treatment ◽  
Electric Field ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Pectin Methylesterase ◽  
Pulsed Electric Field ◽  
Citrus Juices ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. The pectin methylesterase activity and the acceptability of nine juices obtained from Clementine mandarins, Valencia oranges, and Ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85°C for 10 s), and treated by pulsed electric fields (25 kV/cm for 330 μs), were evaluated. The treatments, selected to reach a similar level of pectin methylesterase inactivation, produced juices that did not differ in acceptability from each other for the three varieties and in all cases were less acceptable than the untreated juice.

scholarly journals Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1132 ◽  
Author(s):  
Philip M. Graybill ◽  
Rafael V. Davalos
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Current Understanding ◽  
Vascular Effects ◽  
Cell Substrate ◽  
Membrane Effects ◽  
Cytoskeletal Disruption

Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies.

Inactivation of tomato juice peroxidase by high-intensity pulsed electric fields as affected by process conditions

2008 ◽  
Vol 107 (2) ◽  
pp. 949-955 ◽  
Author(s):  
Ingrid Aguiló-Aguayo ◽  
Isabel Odriozola-Serrano ◽  
Luciano José Quintão-Teixeira ◽  
Olga Martín-Belloso
Keyword(s):  
Electric Fields ◽  
High Intensity ◽  
Pulsed Electric Fields ◽  
Tomato Juice ◽  
Process Conditions

Modeling changes in health-related compounds of tomato juice treated by high-intensity pulsed electric fields

2008 ◽  
Vol 89 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Isabel Odriozola-Serrano ◽  
Robert Soliva-Fortuny ◽  
Vicente Gimeno-Añó ◽  
Olga Martín-Belloso
Keyword(s):  
Electric Fields ◽  
High Intensity ◽  
Pulsed Electric Fields ◽  
Tomato Juice ◽  
Health Related ◽  
Related Compounds

Lycopene, Vitamin C, and Antioxidant Capacity of Tomato Juice as Affected by High-Intensity Pulsed Electric Fields Critical Parameters

2007 ◽  
Vol 55 (22) ◽  
pp. 9036-9042 ◽  
Author(s):  
Isabel Odriozola-Serrano ◽  
Ingrid Aguiló-Aguayo ◽  
Robert Soliva-Fortuny ◽  
Vicente Gimeno-Añó ◽  
Olga Martín-Belloso
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Electric Fields ◽  
High Intensity ◽  
Pulsed Electric Fields ◽  
Tomato Juice ◽  
Critical Parameters

scholarly journals Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene

2021 ◽  
Vol 23 (1) ◽  
pp. 451
Author(s):  
Justina Kavaliauskaitė ◽  
Auksė Kazlauskaitė ◽  
Juozas Rimantas Lazutka ◽  
Gatis Mozolevskis ◽  
Arūnas Stirkė
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Electric Field ◽  
Electric Fields ◽  
Mammalian Cells ◽  
Single Gene ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Potential Candidate ◽  
Cell Parameters

The possibility to artificially adjust and fine-tune gene expression is one of the key milestones in bioengineering, synthetic biology, and advanced medicine. Since the effects of proteins or other transgene products depend on the dosage, controlled gene expression is required for any applications, where even slight fluctuations of the transgene product impact its function or other critical cell parameters. In this context, physical techniques demonstrate optimistic perspectives, and pulsed electric field technology is a potential candidate for a noninvasive, biophysical gene regulator, exploiting an easily adjustable pulse generating device. We exposed mammalian cells, transfected with a NF-κB pathway-controlled transcription system, to a range of microsecond-duration pulsed electric field parameters. To prevent toxicity, we used protocols that would generate relatively mild physical stimulation. The present study, for the first time, proves the principle that microsecond-duration pulsed electric fields can alter single-gene expression in plasmid context in mammalian cells without significant damage to cell integrity or viability. Gene expression might be upregulated or downregulated depending on the cell line and parameters applied. This noninvasive, ligand-, cofactor-, nanoparticle-free approach enables easily controlled direct electrostimulation of the construct carrying the gene of interest; the discovery may contribute towards the path of simplification of the complexity of physical systems in gene regulation and create further synergies between electronics, synthetic biology, and medicine.

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