Alteration of flesh color and enhancement of bioactive substances via the stimulation of anthocyanin biosynthesis in ‘Friar’ plum fruit by low temperature and the removal

2020 ◽  
Vol 310 ◽  
pp. 125862 ◽  
Author(s):  
Limin Wang ◽  
Weina Sang ◽  
Ranran Xu ◽  
Jiankang Cao
Keyword(s):  
Low Temperature ◽  
Anthocyanin Biosynthesis ◽  
Flesh Color ◽  
Bioactive Substances ◽  
Stimulation Of

Kinetin-Mediated Stimulation of Accumulation of Buckwheat Flavonoids in the Dark

1983 ◽  
Vol 38 (9-10) ◽  
pp. 711-718 ◽  
Author(s):  
U. Margna ◽  
T. Vainjärv
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Flavonoid Biosynthesis ◽  
Accumulation Rates ◽  
Sharp Rise ◽  
Short Treatment ◽  
Exogenous Substrate ◽  
Percent Increase ◽  
High Level ◽  
Fold Stimulation ◽  
Stimulation Of

A short treatment of excised buckwheat cotyledons with a solution of kinetin lead to an up to 9-fold stimulation of anthocyanin biosynthesis, to an about 50 percent increase in the accumula­tion of rutin, and to an about 30 percent increase, on the average, in the accumulation of C-glycosylflavones in the treated material during its posttreatment incubation in the dark. When the treated cotyledons were incubated in a solution of ʟ--phenylalanine anthocyanin accumulation in the dark practically attained the same high level as it was observed in the illuminated cotyledons fed with exogenous ʟ--phenylalanine. In experiments with l4C-labelled L-phenylalanine kinetin induced a sharp rise in the labelling (resp. in the utilization of exogenous substrate for biosynthesis) of anthocyanins and rutin in the dark and a slight increase in the radioactivity of C-glycosylflavones. Similar labelling changes occurred in the illuminated cotyledons. However, both kinetin and light still more effectively promoted biosynthetic use of the endogenous sub­strate. As a result the relative portion of flavonoids formed from exogenous L-phenylalanine under these conditions showed a decrease as compared with the ratio of precursor use in the un­treated cotyledons. The results show that low accumulation rates of anthocyanins and other flavo­noids in the dark are conditioned by the limited access of substrate (ʟ--phenylalanine) molecules to the flavonoid enzymes lending further support to the idea that flavonoid biosynthesis is normally controlled at the substrate rather than at the enzymic level.

scholarly journals Anti-Skin Cancer Activities of Apostichopus japonicus Extracts from Low-Temperature Ultrasonification Process

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Nam Young Kim ◽  
Woon Yong Choi ◽  
Soo Jin Heo ◽  
Do Hyung Kang ◽  
Hyeon Yong Lee
Keyword(s):  
Skin Cancer ◽  
Low Temperature ◽  
Apostichopus Japonicus ◽  
Human Keratinocytes ◽  
Malignant Cell ◽  
Extraction Process ◽  
Hot Water ◽  
Skin Tumor ◽  
Bioactive Substances ◽  
Marine Biomass

Objectives. This work aimed to enhance anti-skin cancer activities of Apostichopus japonicus, spiky sea cucumber, through ultrasonification extraction process at low temperature. Methods. Dried Apostichopus japonicus was extracted with an ultrasonification process at 50°C and 95 kHz for two hours (UE), and anti-skin cancer activities of the extract from the UE were also compared with those from conventional extraction processes using hot water (WE) or 70% ethanol at 80°C (EE) for 12 hours. Results. The amount of canthaxanthin in the UE was higher than that in the WE or EE, and its cytotoxicity against human keratinocytes was less than the others. The extract from the UE showed 93.5% inhibition against human malignant cell growth, which was also higher than those from both WE and EE. The extract from the UE demonstrated the ability of inhibiting both cancer cell proliferation and metastasis by downregulating the skin tumor-promoting genes such as Bcl-2, STAT3, and MMP-9. Conclusions. The ultrasonification process was proved to be effective especially in extracting heat-sensitive marine biomass, A. japonicus having higher amounts of canthaxanthin and better anti-skin cancer activities, possibly due to less destruction and high elution of bioactive substances under low temperature extraction condition.

CBFs Function in Anthocyanin Biosynthesis by Interacting with MYB113 in Eggplant (Solanum melongena L.)

2019 ◽  
Vol 61 (2) ◽  
pp. 416-426 ◽  
Author(s):  
Lu Zhou ◽  
Yongjun He ◽  
Jing Li ◽  
Yang Liu ◽  
Huoying Chen
Keyword(s):  
Low Temperature ◽  
Anthocyanin Biosynthesis ◽  
Solanum Melongena ◽  
Structural Genes ◽  
Cold Response ◽  
Anthocyanin Pathway ◽  
R2r3 Myb ◽  
Dependent Pathway ◽  
Shed Light ◽  
R2r3 Myb Transcription Factors

Abstract Eggplant is rich in anthocyanins. R2R3-MYB transcription factors play a key role in the anthocyanin pathway. Low temperature is vital abiotic stress that affects the anthocyanin biosynthesis in plants. CBFs (C-repeat binding factors) act as central regulators in cold response. In this study, we found that SmCBF1, SmCBF2 and SmCBF3, via their C-terminal, physically interacted with SmMYB113, a key regulator of anthocyanin biosynthesis in eggplant. SmCBF2 and SmCBF3 upregulated the expression of SmCHS and SmDFR via a SmMYB113-dependent pathway. In addition, the transient expression assays demonstrated that co-infiltrating SmCBFs and SmMYB113 significantly improved the contents of anthocyanin and the expression levels of anthocyanin structural genes in tobacco. When SmTT8, a bHLH partner of SmMYB113, coexpressed with SmCBFs and SmMYB113, the anthocyanin contents were significantly enhanced compared with SmCBFs and SmMYB113. Furthermore, overexpression of SmCBF2 and SmCBF3 could facilitate the anthocyanin accumulation under cold conditions in Arabidopsis. Taken together, these results shed light on the functions of SmCBFs and potential mechanisms of low-temperature-induced anthocyanin biosynthesis in eggplant.

Curing and low-temperature combined post-harvest storage enhances anthocyanin biosynthesis in blood oranges

2020 ◽  
pp. 128334
Author(s):  
Lourdes Carmona ◽  
Berta Alquézar ◽  
Gianfranco Diretto ◽  
Filippo Sevi ◽  
Tatiane Malara ◽  
...  
Keyword(s):  
Low Temperature ◽  
Anthocyanin Biosynthesis ◽  
Post Harvest

THE MULTILAYERED HIGH Tc SUPERCONDUCTORS; THE JOSEPHSON MODEL OF WEAK LINKS

1995 ◽  
Vol 09 (21) ◽  
pp. 2811-2820
Author(s):  
VALERY A. CHERENKOV
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
High Temperature Superconductors ◽  
Weak Links ◽  
High Tc Superconductors ◽  
Main Attention ◽  
Multilayered Structures ◽  
High Tc ◽  
Stimulation Of

The multilayered (N-S(D)-N)-types structures have been observed: there are the traditional Niobium low-temperature superconductors and new high-temperature superconductors. The main attention had been directed to the anisotropy, defectively and the stimulation of the superconductivity in the multilayered structures.

CHEMICAL EFFECTS ON ANTHOCYANIN BIOSYNTHESIS IN STORED CRANBERRIES

1970 ◽  
Vol 50 (6) ◽  
pp. 673-677 ◽  
Author(s):  
P. E. CANSFIELD ◽  
F. J. FRANCIS
Keyword(s):  
Indoleacetic Acid ◽  
Anthocyanin Biosynthesis ◽  
Pigment Production ◽  
Consumer Acceptance ◽  
Anthocyanin Pigment ◽  
Total Pigment ◽  
Chemical Effects ◽  
M 10 ◽  
Pigment Contents ◽  
Stimulation Of

Infiltration of pale colored cranberries with gibberellic acid (10−4M, 10−5M), malathion (0.38 × 10−4M), indoleacetic acid (10−6M) and galactose (10−2M) stimulated the development of anthocyanin pigment. Galactose (0.5 × 10−1M, 0.25 × 10−1M) and sucrose (10−1M, 0.5 × 10−1M, 0.25 × 10−1M) inhibited pigment production. The experimental berries all had much lower total pigment contents than well colored, vine-ripened berries. The ratios of the four main pigments were also different. The stimulation of pigment production was not great enough to influence consumer acceptance. However, the results were sufficiently encouraging to suggest that further experiments were justified.

Effect of Ethylene on the Respiratory Response of Isolated Sweet Potato Mitochondria

1978 ◽  
Vol 5 (3) ◽  
pp. 239 ◽  
Author(s):  
GP Arron ◽  
DA Day ◽  
SD Grover ◽  
GG Laties
Keyword(s):  
Low Temperature ◽  
Sweet Potato ◽  
Redox State ◽  
Plant Hormone ◽  
Plant Tissues ◽  
Respiratory Response ◽  
Apparent Effect ◽  
Uptake Rates ◽  
Stimulation Of

The plant hormone ethylene had no apparent effect on the redox state of the respiratory chain components of sweet potato mitochondria during oxidation of succinate. Low temperature e.p.r. spectra of sweet potato mitochondria, in various respiratory states, were not altered by the presence of ethylene. Oxygen uptake rates and ADP/O ratios of sweet potato mitochondria oxidising malate or succinate were also not affected by ethylene. The role of ethylene in the stimulation of respiration in plant tissues is discussed.

scholarly journals Reactive Oxygen Species Acts as an Important Inducer in Low-temperature-induced Anthocyanin Biosynthesis in Begonia semperflorens

2018 ◽  
Vol 143 (6) ◽  
pp. 486-493 ◽  
Author(s):  
Ying Qu ◽  
Xue Bai ◽  
Yajun Zhu ◽  
Rui Qi ◽  
Geng Tian ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Low Temperature ◽  
Anthocyanin Biosynthesis ◽  
Redox Homeostasis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Superoxide Anions ◽  
Expression Of Genes ◽  
Glutathione Cycle ◽  
Begonia Semperflorens

Leaves of Begonia semperflorens accumulate anthocyanins and turn red under low temperature (LT). In the present work, LT increased H2O2 content and superoxide anions production rate, causing significant increases in the activities of enzymes and contents of reduced components involved in the ascorbate-glutathione cycle (AsA-GSH cycle). As a result, LT-exposed seedlings increased the expression of genes involved in anthocyanin biosynthesis, and accumulated anthocyanin. Based on LT condition, application of N,N'-dimethylthiourea (DMTU) decreased reactive oxygen species (ROS) content, and unbalanced the AsA-GSH-controlled redox homeostasis. As a result, seedlings in the LT + DMTU group did not accumulate anthocyanin. Our results suggest that ROS may act as an important inducer in LT-induced anthocyanin biosynthesis.

scholarly journals miRNA Profiling in the Chicken Liver under the Influence of Early Microbiota Stimulation with Probiotic, Prebiotic, and Synbiotic

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 685
Author(s):  
Michalina Sikorska ◽  
Maria Siwek ◽  
Anna Slawinska ◽  
Aleksandra Dunislawska
Keyword(s):  
Gene Expression ◽  
Mirna Expression ◽  
Regulation Of Gene Expression ◽  
In Ovo ◽  
Bioactive Substances ◽  
Chicken Breed ◽  
Microarray Experiments ◽  
Native Chicken ◽  
Expression Activity ◽  
Stimulation Of

Epigenetic regulation of gene expression is a form of interaction of the external environment on reading and transcription of genetic information encoded in nucleic acids. We provided evidence that early stimulation of the chicken microbiota with in ovo delivered synbiotics influenced gene expression and DNA methylation in the liver. Therefore, we hypothesize that the stimulation of microbiota by administering bioactive substances in ovo also affects the activity of miRNA in liver. For the analysis of miRNA activity, RNA was isolated from liver of adult broiler chicken and native chicken breed. The animals received a prebiotic, probiotic and synbiotic in ovo on day 12 of egg incubation. The analysis of miRNA expression was performed using the LNA method on a miRNA panel selected on the basis of previous microarray experiments. We have found increased miRNA expression activity after probiotic and synbiotic administration, especially in native chicken breed. Our results suggest that prebiotics reduce or do not affect miRNA activity. We have also shown that miRNA activity is regulated by the substance and genotype of the chicken. We can conclude that miRNAs constitute an important component of the molecular mechanism of host–probiotic interaction in liver.

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