Characteristic and antimicrobial resistance in Escherichia coli from retail meats purchased in the Czech Republic

Food Control ◽  
2015 ◽  
Vol 47 ◽  
pp. 401-406 ◽  
Author(s):  
Alena Skočková ◽  
Ivana Koláčková ◽  
Kateřina Bogdanovičová ◽  
Renáta Karpíšková
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Antimicrobial Resistance ◽  
The Czech Republic ◽  
Retail Meats

scholarly journals Antimicrobial Resistance in Fecal Escherichia coli Isolates from Healthy Urban Children of Two Age Groups in Relation to Their Antibiotic Therapy

2011 ◽  
Vol 55 (6) ◽  
pp. 3005-3007 ◽  
Author(s):  
Ivan Literak ◽  
Radim Petro ◽  
Monika Dolejska ◽  
Erika Gruberova ◽  
Hana Dobiasova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Antimicrobial Resistance ◽  
Age Groups ◽  
Antibiotic Resistant ◽  
The Czech Republic ◽  
Content Type ◽  
E Coli ◽  
Significant Difference ◽  
Two Age Groups

ABSTRACTThe study was performed in the Czech Republic during 2007 to 2009. OfEscherichia coliisolates from 275 children aged 6 weeks, 36% (n= 177) were resistant to 1 to 7 antibiotics. Of isolates from 253 children aged 6 to 17 years, 24% (n= 205) were resistant to 1 to 5 antibiotics. There was no significant difference in the prevalences of antibiotic-resistantE. coliisolates between these groups of children, even though the consumptions of antibiotics were quite different.

scholarly journals Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 838
Author(s):  
Alžběta Kalová ◽  
Tereza Gelbíčová ◽  
Søren Overballe-Petersen ◽  
Eva Litrup ◽  
Renáta Karpíšková
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Antimicrobial Resistance ◽  
Coli Strain ◽  
Bacterial Strains ◽  
Asian Origin ◽  
The Czech Republic ◽  
E Coli ◽  
Antimicrobial Resistance Genes ◽  
Aquaculture Products

Aquaculture systems are widely recognised as hotspots for horizontal gene transfer, and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, and IncX4, as well as on the rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, and on the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains, pointing to the possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that some aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points to the risks associated with the stabilisation of the mcr genes in the bacterial chromosome.

Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

2013 ◽  
Vol 167 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Alena Skočková ◽  
Renáta Karpíšková ◽  
Ivana Koláčková ◽  
Šárka Cupáková
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Retail Market ◽  
The Czech Republic

scholarly journals  Faecal shedding of verotoxigenic Escherichia coli in cattle in the Czech Republic

2011 ◽  
Vol 56 (No. 4) ◽  
pp. 149-155 ◽  
Author(s):  
P. Alexa ◽  
L. Konstantinova ◽  
Z. Sramkova-Zajacova
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Dairy Cattle ◽  
Age Groups ◽  
Immunomagnetic Separation ◽  
The Other ◽  
Somatic Antigen ◽  
The Czech Republic ◽  
E Coli ◽  
One Year

A survey to estimate the prevalence of verotoxigenic E. coli (VTEC) or enterohaemorrhagic E. coli (EHEC) in rectal swabs from healthy dairy cattle aged three weeks, three months and one year was conducted in three herds from the Czech Republic. Screening for the presence of the stx1, stx2 and eaeA genes in faecal swab cultures was performed by PCR, and in positive samples, isolated colonies were examined. Immunomagnetic separation was used for the isolation of the VTEC serogroup O157 from samples. VTEC were detected in animals from all three herds under study. In the group of 3-week-old calves, VTEC were only detected in samples collected in the summer months. However, in the other age-groups, VTEC were detected in both the summer and winter months. EHEC shedding was observed in 30 to 100% of the total samples collected from cattle aged three months and one year in the summer months, and in 30 to 60% of samples taken in the winter months. EHEC strains of serogroup O157 were detected in two herds. The range of verotoxins shed by VTEC isolates of serogroup O157 differed between herds. Besides serogroup O157, additional EHEC belonging to the antigen groups O26, O103, O128 and O153 have been identified, and in some of them, no somatic antigen was detected.

scholarly journals Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73927 ◽  
Author(s):  
Monika Marejková ◽  
Květa Bláhová ◽  
Jan Janda ◽  
Angelika Fruth ◽  
Petr Petráš
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Hemolytic Uremic Syndrome ◽  
Enterohemorrhagic Escherichia Coli ◽  
The Czech Republic ◽  
Uremic Syndrome

scholarly journals Genomic analysis of Escherichia coli strains isolated from diseased chicken in the Czech Republic

2019 ◽  
Author(s):  
Aneta Papoušková ◽  
Martina Masaříková ◽  
Adam Valček ◽  
David Šenk ◽  
Darina Čejková ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Economic Losses ◽  
Zoonotic Potential ◽  
Beta Lactamase ◽  
Phylogenetic Groups ◽  
The Czech Republic ◽  
E Coli ◽  
Molecular Features ◽  
Local Spread

Abstract Background: Avian pathogenic Escherichia coli (APEC) can cause various extraintestinal infections in poultry, resulting in massive economic losses in poultry industry. In addition, some avian E. coli strains may have zoonotic potential, making poultry a possible source of infection for humans. Due to its extreme genetic diversity, this pathotype remains poorly defined. This study aimed to investigate the diversity of colibacillosis-associated E. coli isolates from Central European countries with a focus on the Czech Republic. Results: Of 95 clinical isolates subjected to preliminary characterization, 32 were selected for whole-genome sequencing. A multi resistant phenotype was detected in a majority of the sequenced strains with the predominant resistance to β -lactams and quinolones being associated with TEM-type beta-lactamase genes and chromosomal gyrA mutations respectively. The phylogenetic analysis confirmed a great diversity of isolates, that were derived from nearly all phylogenetic groups, with predominace of B2, B1 and C phylogroups. Clusters of closely related isolates within ST23 (phylogroup C) and ST429 (phylogroup B2) indicated a possible local spread of these clones. Besides, the ST429 cluster carried blaCMY-2, -59 genes for AmpC beta-lactamase and isolates of both clusters were generally well-equipped with virulence-associated genes, with considerable differences in distribution of certain virulence-associated genes between phylogenetically distant lineages. Other important and potentially zoonotic APEC STs were detected, incl. ST117, ST354 and ST95, showing several molecular features typical for human ExPEC.Conclusions: The results support the concept of local spread of virulent APEC clones, as well as of zoonotic potential of specific poultry-associated lineages, and highlight the need to investigate the possible source of these pathogenic strains.

scholarly journals Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hßtrains in the Czech Republic

1998 ◽  
Vol 36 (7) ◽  
pp. 2135-2137 ◽  
Author(s):  
Martina Bielaszewska ◽  
Herbert Schmidt ◽  
Mohamed A. Karmali ◽  
Rasik Khakhria ◽  
Jan Janda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
The Czech Republic ◽  
Isolation And Characterization ◽  
First Finding ◽  
Uremic Syndrome ◽  
Genotypic Characteristics

Two sorbitol-fermenting (SF) Shiga toxin-producingEscherichia coli (STEC) O157:H− strains were isolated from patients with hemolytic-uremic syndrome in the Czech Republic in 1995. Their phenotypic and genotypic characteristics and genomic DNA fingerprints were identical or closely related to those of SF STEC O157:H− strains isolated in Germany in 1988 to 1997. This indicates that the Czech isolates belong to the SF STEC O157 clone which is widespread in Germany. It is the first finding of the clone outside Germany.

scholarly journals Gene typing of the colonisation factor K88 (F4) in enterotoxigenic Escherichia coli strains isolated from diarrhoeic piglets

2001 ◽  
Vol 46 (No. 2) ◽  
pp. 46-49 ◽  
Author(s):  
P. Alexa ◽  
K. Štouračová ◽  
J. Hamřík ◽  
I. Rychlík
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Enterotoxigenic Escherichia Coli ◽  
Chain Reaction ◽  
The Czech Republic ◽  
E Coli ◽  
Gene Typing ◽  
Brush Borders ◽  
Variant Genotype ◽  
Polymerase Chain

More than 4 000 E. coli strains isolated from diarrhoeic piglets in 111 pig herds in the Czech Republic during the period 1995–2000 were examined for serogroup and virulence factors. Gene typing of the K88 marker by polymerase chain reaction (PCR) was used for the examination of 283 enterotoxigenic strains (ETEC) which agglutinated with antisera against K88 or adhered to intestinal brush borders. The K88 gene was detected in 237 strains; among them 232 strains possesed the K88 variant. Genotype K88ab was found in two strains of the serogroup O8 from one herd and the gene K88ad was detected in three strains of the serogroup O8 originating from another herd. The results show that the type K88ac is predominant in ETEC strains with colonisation factors K88 in pig herds in the Czech Republic.

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