Interspecies variation in biofilm-forming capacity of psychrotrophic bacterial isolates from Chinese raw milk

Food Control ◽  
2018 ◽  
Vol 91 ◽  
pp. 47-57 ◽  
Author(s):  
Lei Yuan ◽  
Mette Burmølle ◽  
Faizan A. Sadiq ◽  
Ni Wang ◽  
Guoqing He
Keyword(s):  
Raw Milk ◽  
Bacterial Isolates ◽  
Interspecies Variation

scholarly journals Prevalence of Mastitis and Antibiotic Resistance of Bacterial Isolates from CMT Positive Milk Samples Obtained from Dairy Cows, Camels, and Goats in Two Pastoral Districts in Southern Ethiopia

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1530
Author(s):  
Amanuel Balemi ◽  
Balako Gumi ◽  
Kebede Amenu ◽  
Sisay Girma ◽  
Muuz Gebru ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Dairy Cows ◽  
Raw Milk ◽  
Multidrug Resistant ◽  
Southern Ethiopia ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococcus ◽  
Bacterial Isolates ◽  
E Coli ◽  
Milk Samples

A study was carried out from August 2017 to February 2018 on lactating dairy cows, one-humped dromedary camels, and goats to determine mastitis in the Bule Hora and Dugda Dawa districts of in Southern Ethiopia. Milk samples from 564 udder quarters and udder halves from 171 animals consisting of 60 dairy cows, 51 camels, and 60 goats were tested for mastitis. Sixty-four positive udder milk samples were cultured, and bacterial mastitis pathogens were isolated and identified. The antibiotic resistance of bacterial isolates from milk with mastitis was tested against nine antimicrobials commonly used in the study area. Cow- and quarter-level prevalence of mastitis in dairy cows, camels, and goats was 33.3%, 26.3%, and 25% and 17.6%, 14.5%, and 20%, respectively. In cattle, the prevalence was significantly higher in Dugda Dawa than in Bule Hora. Major bacterial isolates were coagulase-negative Staphylococcus species (39.1%), S. aureus (17.2%), S. hyicus (14.1%), and S. intermedius and Escherichia coli (9.4% each). In camels, udder abnormality and mastitis were significantly higher in late lactation than in early lactation. Mastitis tends to increase with parity in camels. E. coli isolates were highly resistant to spectinomycin, vancomycin, and doxycycline, whereas most S. aureus isolates were multidrug-resistant. Most of the rural and periurban communities in this area consume raw milk, which indicates a high risk of infection with multidrug-resistant bacteria. We recommend a community-focused training program to improve community awareness of the need to boil milk and the risk of raw milk consumption.

Proteolysis produced within biofilms of bacterial isolates from raw milk tankers

2012 ◽  
Vol 157 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Koon Hoong Teh ◽  
Steve Flint ◽  
Jon Palmer ◽  
Paul Andrewes ◽  
Phil Bremer ◽  
...  
Keyword(s):  
Raw Milk ◽  
Bacterial Isolates

scholarly journals Identification of ovine-associated staphylococci by MALDI-TOF mass spectrometry

2021 ◽  
Author(s):  
A. Tonamo ◽  
I. Komlósi ◽  
L. Varga ◽  
M. Kačániová ◽  
F. Peles
Keyword(s):  
Mass Spectrometry ◽  
Raw Milk ◽  
Bacterial Isolates ◽  
Bulk Tank Milk ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bulk Tank ◽  
Raw Milk Cheese ◽  
Tof Ms

AbstractThe objective of this study was to use matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) for the identification of ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank milk, and cheese samples and were characterised by conventional phenotypic methods. A total of 69 bacterial isolates were further confirmed by MALDI-TOF MS. Forty-two (60.9%) of 69 isolates were successfully identified on genus and species level. Two thirds (n = 28) of the 42 identified isolates were shown to be Staphylococcus spp. These 28 staphylococcal isolates formed two clusters, one consisting of 22 Staphylococcus aureus strains and the other composed of 6 non-aureus staphylococci, including S. simulans (n = 3), S. auricularis, S. equorum, and S. haemolyticus. MALDI-TOF MS has proven to be a reliable tool for the identification of staphylococci from raw sheep's milk, especially bulk tank milk; however, currently it appears to be less useful for the identification of bacterial isolates originating from ovine US samples. This is the first study to evaluate the applicability of MALDI-TOF MS for identification of Staphylococcus spp. in ovine raw milk, cheese, and US samples in Hungary.

scholarly journals Genetic Diversity and The Presence of Circular Plasmids in Bacillus Cereus Isolates of Clinical and Food Origin

2021 ◽  
Author(s):  
Tjaša Cerar Kišek ◽  
Nežka Pogačnik ◽  
Karmen Godič Torkar
Keyword(s):  
Bacillus Cereus ◽  
Raw Milk ◽  
Clear Correlation ◽  
Bacterial Isolates ◽  
Pfge Analysis ◽  
Linear Plasmids ◽  
Clinical Specimens ◽  
Milk Samples ◽  
Stool Specimens ◽  
Pfge Profiles

Abstract The diversity of 61 Bacillus cereus strains isolated from different clinical specimens, food including raw milk and milk products, and water was evaluated. PFGE analysis could discriminate 61 distinct pulsotypes with similarity levels from 25 to 82%, which were divided into 13 clonal complexes. The similarity between clonal complexes was at least 40%. Clinical strains were divided into 10 clonal complexes, while the strains, isolated from milk, food and water were included in 9, 6 and 6 clonal complexes, respectively. Three clonal complexes were dominated by clinical isolates, while they were absent in two complexes. Bacterial isolates from foods, being a probable source of alimentary toxoinfection, showed low similarity to isolates from stool specimens. The isolates from both sources were classified together in only 4 out of 13 clonal complexes. The large circular and linear plasmids with the sizes between 50 and 200 kb were detected in 24 (39.3%) and 14 (23%) B. cereus strains, respectively. Thirteen (21.3%) strains contained only one plasmid, two plasmids were found in 6 (9.8%) of strains, and three or more plasmids were obtained in 5 (8.2%) of tested strains. The plasmids were confirmed in 30.8% and 40% of isolates from clinical specimens and food and milk samples, respectively. No clear correlation between the PFGE profiles, the source as well as plasmid content among all tested strains was observed.

scholarly journals Detection of mycobacteria in coalho cheese sold in Northeastern Brazil

2021 ◽  
pp. 439-446
Author(s):  
Giovanni Brito Medeiros ◽  
◽  
Onaldo Guedes Rodrigues ◽  
Pirajá Saraiva Bezerra Neto ◽  
Rosália Severo de Medeiros ◽  
...  
Keyword(s):  
Agricultural Practices ◽  
Raw Milk ◽  
Northeastern Brazil ◽  
Bacterial Isolates ◽  
Tuberculosis Complex ◽  
Production Chain ◽  
Two Samples ◽  
Good Manufacturing Practices ◽  
Cheese Production ◽  
Mycobacterium Spp

The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).

Use of Raw Milk Persists Despite Rabies Scare

2006 ◽  
Vol 40 (11) ◽  
pp. 21
Author(s):  
JOHN R. BELL
Keyword(s):  
Raw Milk

Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens

2020 ◽  
Author(s):  
Marita Vedovelli CARDOZO ◽  
Natalia NESPOLO ◽  
Tammy Chioda DELFINO ◽  
Camila Chioda de ALMEIDA ◽  
Lucas José Luduverio PIZAURO ◽  
...  
Keyword(s):  
Raw Milk ◽  
Infection Source ◽  
Raw Milk Cheese

The comparative study among the MRSAcin, Nisin A and vancomycin, on biofilm formation by Methicillin resistance Staphylococcus aureus isolated from food sources.

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
¹Hind H. Muunim ◽  
Muna T Al-Mossawei ◽  
Mais Emad Ahmed
Keyword(s):  
Biofilm Formation ◽  
Methicillin Resistance ◽  
Microbial Control ◽  
Antibiotic Sensitivity ◽  
Raw Milk ◽  
Sensitivity Test ◽  
Food Sources ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Food Preservatives

Biofilms formation by pathogens microbial Control considered important in medical research because it is the hazarded virulence factor leading to becoming difficult to treat because of its high resistance to antimicrobials. Glycopeptide antibiotic a (Vancomycin) and the commercial bacteriocin (Nisin A) were used to comparative with purification bacteriocin (MRSAcin) against MRSA biofilm. One hundred food samples were collected from Baghdad markets from July 2016 to September 2016, including (cheese, yogurt, raw milk, fried meat, grilled meat, and beef burger). All samples were cultures; S. aureus was confirmation by macroscopic culture and microscopic examination, in addition to biochemical tests. Methicillin resistance S. asureus (MRSA) were identification by antibiotic sensitivity test (AST), Vitek 2 system. The result shown the 60(60%) isolate were identified as S. aureus and 45(75%) gave positive result as MRSA isolate, M13 isolate was chosen as MRSA isolates highest biofilm formation for treatment with MRSAcin, Nisin A(bacteriocin) and Vancomycin (antibiotic) to compared the more antimicrobial have bacteriocidal effect. The sensitivity test uses to determine the effect of MRSAcin, Nisin A, and Vancomycin MIC on MRSA planktonic cell by (WDA). The new study shows the impacts of new kind Pure Bacteriocins (MRSAcin) from methicillin-resistant S. aureus (MRSA) highly effects then (Vancomycin and Nisin A) at different concentration. In a current study aimed to suggest new Bacteriocin is potent highly for the treatment of resistant bacteria biofilm infections in food preservatives

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