Effect of protein topology on hierarchical complexation of epsilon-polylysine and protein: A multiscale structural analysis

2022 ◽  
Vol 125 ◽  
pp. 107431
Author(s):  
Liang Guo ◽  
Jie Chen ◽  
Sheng Fang ◽  
Yan-hua Li ◽  
Yi-chao Song ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Protein A ◽  
Protein Topology

scholarly journals Structural Analysis of Human Replication Protein A

1995 ◽  
Vol 270 (9) ◽  
pp. 4534-4543 ◽  
Author(s):  
Xavier V. Gomes ◽  
Marc S. Wold
Keyword(s):  
Structural Analysis ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein

scholarly journals Expression, Purification and Structural Analysis of Human IL-18 Binding Protein: A Potent Therapeutic Molecule for Allergy

2008 ◽  
Vol 57 (4) ◽  
pp. 367-376 ◽  
Author(s):  
Takeshi Kimura ◽  
Zenichiro Kato ◽  
Hidenori Ohnishi ◽  
Hidehito Tochio ◽  
Masahiro Shirakawa ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Binding Protein ◽  
Protein A

scholarly journals Structural Analysis of Replication Protein A Recruitment of the DNA Damage Response Protein SMARCAL1

Biochemistry ◽  
2014 ◽  
Vol 53 (18) ◽  
pp. 3052-3061 ◽  
Author(s):  
Michael D. Feldkamp ◽  
Aaron C. Mason ◽  
Brandt F. Eichman ◽  
Walter J. Chazin
Keyword(s):  
Dna Damage ◽  
Structural Analysis ◽  
Dna Damage Response ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Damage Response

Structural Analysis and Lipid-Binding Properties of Recombinant Human Surfactant Protein A Derived from One or Both Genes†

Biochemistry ◽  
2002 ◽  
Vol 41 (47) ◽  
pp. 14041-14053 ◽  
Author(s):  
I. García-Verdugo ◽  
G. Wang ◽  
J. Floros ◽  
C. Casals
Keyword(s):  
Structural Analysis ◽  
Protein A ◽  
Surfactant Protein ◽  
Lipid Binding ◽  
Surfactant Protein A ◽  
Binding Properties

scholarly journals Sequence and structural analysis of COVID-19 E and M protein with MERS virus E and M protein—A comparative study

2021 ◽  
pp. 101023
Author(s):  
Ebtisam A. Aldaais ◽  
Subha Yegnaswamy ◽  
Fatimah Albahrani ◽  
Fatima Alsowaiket ◽  
Sarah Alramadan
Keyword(s):  
Structural Analysis ◽  
Comparative Study ◽  
Protein A ◽  
M Protein

scholarly journals Structural Analysis and Epitope Prediction of MHC Class-1-Chain Related Protein-A for Cancer Vaccine Development

Vaccines ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Tayo Adekiya ◽  
Raphael Aruleba ◽  
Sbonelo Khanyile ◽  
Priscilla Masamba ◽  
Babatunji Oyinloye ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Cancer Vaccine ◽  
Vaccine Development ◽  
Protein A ◽  
Epitope Prediction ◽  
Related Protein ◽  
Class 1

Structural analysis of the diadenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3′-dATP

2015 ◽  
Vol 469 (3) ◽  
pp. 367-374 ◽  
Author(s):  
Martina Müller ◽  
Tobias Deimling ◽  
Karl-Peter Hopfner ◽  
Gregor Witte
Keyword(s):  
Structural Analysis ◽  
Thermotoga Maritima ◽  
Bacterial Species ◽  
Protein A ◽  
Dna Integrity

Structures of Thermotoga maritima DNA-integrity-scanning protein A (DisA) in different reaction states describe the diadenylate cyclase (DAC) reaction and the possibility of its inhibition. We conclude that the mechanisms of cyclic-di-AMP (c-di-AMP) synthesis and its inhibition are conserved among different DAC enzymes and bacterial species.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Structural analysis of the surface-layer protein of spirillum serpens by high-resolution electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 738-739
Author(s):  
W. H. Wu ◽  
R. M. Glaeser
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Structural Analysis ◽  
High Resolution ◽  
Low Dose ◽  
High Resolution Electron Microscopy ◽  
Optical Diffraction ◽  
Surface Layer Protein ◽  
Morphological Unit ◽  
Resolution Electron

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.

Presence of antibody in virus-infected brain cells of SSPE ferrets

1983 ◽  
Vol 41 ◽  
pp. 804-805
Author(s):  
Hannah R. Brown ◽  
Tammy L. Donato ◽  
Halldor Thormar
Keyword(s):  
Measles Virus ◽  
Plasma Cells ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Neutralizing Antibody ◽  
Brain Cells ◽  
Antibody Titers ◽  
A Cell ◽  
The Central Nervous System ◽  
The Brain

Measles virus specific immunoglobulin G (IgG) has been found in the brains of patients with subacute sclerosing panencephalitis (SSPE), a slowly progressing disease of the central nervous system (CNS) in children. IgG/albumin ratios indicate that the antibodies are synthesized within the CNS. Using the ferret as an animal model to study the disease, we have been attempting to localize the Ig's in the brains of animals inoculated with a cell associated strain of SSPE. In an earlier report, preliminary results using Protein A conjugated to horseradish peroxidase (PrAPx) (Dynatech Diagnostics Inc., South Windham, ME.) to detect antibodies revealed the presence of immunoglobulin mainly in antibody-producing plasma cells in inflammatory lesions and not in infected brain cells.In the present experiment we studied the brain of an SSPE ferret with neutralizing antibody titers of 1:1024 in serum and 1:512 in CSF at time of sacrifice 7 months after i.c. inoculation with SSPE measles virus-infected cells. The animal was perfused with saline and portions of the brain and spinal cord were immersed in periodate-lysine-paraformaldehyde (P-L-P) fixative. The ferret was not perfused with fixative because parts of the brain were used for virus isolation.

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