Whey protein and phenolic compound complexation: Effects on antioxidant capacity before and after in vitro digestion

In this study, dark chocolates (DCh) containing zinc lactate (ZnL) were enriched with extracts from elderberries (EFrE), elderflowers (EFlE), and chokeberries (ChFrE) to improve their functional properties. Both dried plant extracts and chocolates were analyzed for antioxidant capacity (AC) using four different analytical methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric ion-reducing antioxidant capacity (CUPRAC), and ferric-reducing antioxidant power (FRAP), while total phenolic content (TPC) was determined by Folin–Ciocalteu (F–C) assay. An increase in antioxidant properties of fortified chocolates was found, and the bioaccessibility of their antioxidants was evaluated. The highest AC and TPC were found in ChFrE and chocolate with chokeberries (DCh + ChFrE) before and after simulated in vitro digestion. Bioaccessibility studies indicated that during the simulated digestion the AC of all chocolates reduced significantly, whereas insignificant differences in TPC results were observed between chemical and physiological extracts. Moreover, the influence of plant extracts on physicochemical parameters such as moisture content (MC), fat content (FC), and viscosity of chocolates was estimated. Furthermore, scanning electron microscopy with dispersive energy spectroscopy (SEM-EDS) was used to analyze surface properties and differences in the chemical composition of chocolates without and with additives.

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Stability of the total antioxidant capacity of fresh fruit juices subjected to an in vitro digestion procedure

Proceedings of The Nutrition Society ◽

10.1017/s0029665110003757 ◽

2010 ◽

Vol 69 (OCE6) ◽

Author(s):

L. Ryan ◽

S. Prescott

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

In Vitro Digestion ◽

Fruit Juices ◽

Fresh Fruit ◽

Digestion Procedure ◽

Total Antioxidant

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In vitro digestion of infant formula model systems: Influence of casein to whey protein ratio

International Dairy Journal ◽

10.1016/j.idairyj.2021.105008 ◽

2021 ◽

Vol 117 ◽

pp. 105008

Author(s):

Arissara Phosanam ◽

Jayani Chandrapala ◽

Thom Huppertz ◽

Benu Adhikari ◽

Bogdan Zisu

Keyword(s):

Whey Protein ◽

Infant Formula ◽

In Vitro Digestion ◽

Model Systems ◽

Protein Ratio

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The effect of processing techniques on the antiproliferative activity of blueberry phenolics before and after in vitro digestion

Journal of Food Processing and Preservation ◽

10.1111/jfpp.16140 ◽

2021 ◽

Author(s):

Nikolina Liović ◽

Vedrana Čikeš‐Čulić ◽

Željana Fredotović ◽

Greta Krešić ◽

Tea Bilušić

Keyword(s):

Antiproliferative Activity ◽

In Vitro Digestion ◽

Before And After ◽

Processing Techniques

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Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.3586 ◽

2018 ◽

Vol 87 (2) ◽

Cited By ~ 2

Author(s):

Anita Agnieszka Śliwińska ◽

Katarzyna Sykłowska-Baranek ◽

Anita Kośmider ◽

Sebastian Granica ◽

Karolina Miszczak ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Cytotoxic Activity ◽

Phenolic Compound ◽

Plant Extracts ◽

Detrimental Effect ◽

A549 Cells ◽

Ferulic Acids

In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of P. filicifolia extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.

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