Quantifying the impact of eight unit operations on the survival of eight Bacillus strains with claimed probiotic properties

Tomato is a vegetable crop with probiotic interest. Currently subject to a global biosecurity emergency due to the epidemic caused by COVID-19, humanity is seeking to maintain its health and become stronger by eating vegetables that have probiotic properties. Considering the request of tomato farmers in the Comarca Lagunera (CL) region, the objective of this work consisted of determining the impact of bioinoculation with Azospirillum brasilense (Ab) and solarized manure (M) on the yield and phytochemical quality of tomato fruits produced in shade mesh. Seeds of the saladette variety TOP 2299 were inoculated with Ab at 1 × 108 CFU.mL. Before 46 days after being sowed, seedlings were transplanted in soil enriched with manure solarized at a rate of 0, 40, 80, 120 and 160 t ha−1; a chemical fertilization (CHF) treatment was also adopted (366-95-635). Emergence, growth, root length, bromatological studies (protein and lipids in plant), yield and organoleptic (Vit C, phenols, flavonoids and lycopene) variables were considered. The results show that biofertilization based on Ab + M40 can be an alternative to produce tomato in shade-house conditions in the CL compared with non-inoculated and CHF treatments.

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Behavior of different Bacillus strains with claimed probiotic properties throughout processed cheese (“requeijão cremoso”) manufacturing and storage

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2019.108288 ◽

2019 ◽

Vol 307 ◽

pp. 108288 ◽

Cited By ~ 7

Author(s):

Mariana B. Soares ◽

Carine N. Almada ◽

Caroline N. Almada ◽

Rafael C.R. Martinez ◽

Eliene P.R. Pereira ◽

...

Keyword(s):

Probiotic Properties ◽

Processed Cheese ◽

And Storage ◽

Bacillus Strains

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Optimizing patient scheduling for ambulatory chemotherapy.

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.31_suppl.199 ◽

2013 ◽

Vol 31 (31_suppl) ◽

pp. 199-199

Author(s):

Ben de Mendonca ◽

Kirsty Wield ◽

Angela Boudreau ◽

Simron Singh ◽

Matthew C. Cheung ◽

...

Keyword(s):

Intensity Variation ◽

Time Of Day ◽

Patient Scheduling ◽

Process Scheduling ◽

Data Infrastructure ◽

Continuous Quality ◽

Unit Operations ◽

Number Of Patients ◽

The Mean ◽

The Impact

199 Background: The Odette Cancer Centre (OCC) manages more than 24,000 chemotherapy visits annually. The delivery process is complex and patients have significant wait times for treatment. The OCC was faced with improving this process with no data infrastructure to support continuous quality improvement. Methods: An electronic scheduling manager, Chemotherapy Appointment Reservation Manager (CHARM) was built in house to improve scheduling logic and optimize bed and chair utilization. The chemotherapy unit has recently undergone renovations to change the staff-to-patient ratio and chair distribution. At baseline, a nurse is assigned to 4 chairs in a “pod” without adjustment for patient and chemotherapy intensity variation. An interprofessional team participated in a Kaizen event to create a Value Stream Map of the scheduling process. Scheduling logic considerations were identified to better match nursing and chair resources to patient appointment times. An analysis was performed to evaluate the distribution of patients throughout the chemotherapy unit by time of day, and day of week to identify opportunities to align the schedule with nursing and pharmacy resources. Results: The mean number of patients seen per day was 85 with a range of 65 to 105. 80% of patients are scheduled before 11:30 (the unit operations 08:30 to 18:00). The mean number of patients assigned to a pod was 8 with a range of 3 to 15. Unit performance on days of >95 patients was observed to be poorest. Load levelling techniques were established to reduce the range of patients booked per day throughout the week. New considerations for scheduling are: maximum 12 patients per nurse per pod per day, maximum 3 new patients per nurse per pod per day, maximum 10 clinical trials per day, and maximum 50% of patients scheduled before 11:30 per day. Conclusions: Matching the patient schedule to the nursing and pharmacy resources of the unit is critical to efficient and safe chemotherapy delivery. A Plan-Do-Study-Act is scheduled for September 2013 to implement the scheduling changes and evaluate the impact of the new logic on unit operations. Further work to improve the delivery process and pharmacy medication processing is ongoing.

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Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri

Microorganisms ◽

10.3390/microorganisms9081590 ◽

2021 ◽

Vol 9 (8) ◽

pp. 1590

Author(s):

Tsukasa Shiraishi ◽

Shintaro Maeno ◽

Sayoko Kishi ◽

Tadashi Fujii ◽

Hiroki Tanno ◽

...

Keyword(s):

Molecular Size ◽

Lipoteichoic Acid ◽

Glycoside Hydrolase Family ◽

Probiotic Properties ◽

Lactobacillus Gasseri ◽

Metabolic Systems ◽

Metabolic Properties ◽

The Impact ◽

Hydrolase Family ◽

Cells Cultured

Lactobacillus gasseri and Lactobacillus paragasseri are human commensal lactobacilli that are candidates for probiotic application. Knowledge of their oligosaccharide metabolic properties is valuable for synbiotic application. The present study characterized oligosaccharide metabolic systems and their impact on lipoteichoic acid (LTA) production in the two organisms, i.e., L. gasseri JCM 1131T and L. paragasseri JCM 11657. The two strains grew well in medium with glucose but poorly in medium with raffinose, and growth rates in medium with kestose differed between the strains. Oligosaccharide metabolism markedly influenced their LTA production, and apparent molecular size of LTA in electrophoresis recovered from cells cultured with glucose and kestose differed from that from cells cultured with raffinose in the strains. On the other hand, more than 15-fold more LTA was observed in the L. gasseri cells cultured with raffinose when compared with glucose or kestose after incubation for 15 h. Transcriptome analysis identified glycoside hydrolase family 32 enzyme as a potential kestose hydrolysis enzyme in the two strains. Transcriptomic levels of multiple genes in the dlt operon, involved in D-alanine substitution of LTA, were lower in cells cultured with raffinose than in those cultured with kestose or glucose. This suggested that the different sizes of LTA observed among the carbohydrates tested were partly due to different levels of alanylation of LTA. The present study indicates that available oligosaccharide has the impact on the LTA production of the industrially important lactobacilli, which might influence their probiotic properties.

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Evaluation of Antagonistic Bacillus and Biochar against Soybean Root Rot Caused by Fusarium oxysporum

International Journal of Agriculture and Biology ◽

10.17957/ijab/15.1836 ◽

2021 ◽

Vol 26 (02) ◽

pp. 287-293

Author(s):

Fan Yang

Keyword(s):

Root Rot ◽

Fusarium Species ◽

Dry Weight ◽

Control Measures ◽

Soybean Root ◽

Soybean Seedlings ◽

Yield And Quality ◽

Soybean Plants ◽

The Impact ◽

Bacillus Strains

Soybean root rot is a worldwide soil-borne fungal disease threatening soybean production, causing huge losses in yield and quality of soybean. Fusarium species are well recognized as the important causal agent of Fusarium root rot. To screen the beneficial Bacillus strains with capability of suppressing soybean root rot and evaluate the impact of Bacillus combined with biochar against soybean root rot, a pot experiment was conducted with different treatments. In this study, as potential biological control measures, antagonistic Bacillus isolates and different types of biochar were added to soil separately and excellent antagonistic strains mixed with bamboo biochar were applied to the soil. The results showed that seven Bacillus strains promoted the growth of soybean seedlings and reduced root rot severity by 33 to 61%. Bacillus amylolique faciens NH2 was associated with the lowest incidence of soybean root rot, indicating its bio-control potential. The value of plant height, root length and plant dry weight of soybean in the sterilized soil mixed with biochar separately treatment were superior to those of soybean in the inoculated with pathogen treatment, especially the bamboo biochar treatment reduced soybean root rot caused by Fusarium significantly and which bio-control efficacy was 77.41%. The soybean plants shoot and root dry weights in the biochar mixed B. amylolique faciens NH2 or B. subtilis DBK treatments were increased by17.1, 10.7% and 19.51, 19.64%, respectively, which were significantly higher than those of the inoculated pathogen treatment. Compared to antagonistic strain or biochar individual treatments, the disease control efficiency on soybean root rot was up to 64.86% in NH2 strain mixed with bamboo biochar treatment, which reduced root rot severity significantly and showed a synergistic effect. These results suggest that antagonistic Bacillus strains mixed with biochar can be used as an effective alternative in managing soybean root rot. © 2021 Friends Science Publishers

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Evaluation of Potential Probiotic Properties of Lactobacillus and Bacillus Strains Derived from Various Sources for Their Potential Use in Swine Feeding

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-021-09861-w ◽

2021 ◽

Author(s):

Nuntiya Pahumunto ◽

Gunnar Dahlen ◽

Rawee Teanpaisan

Keyword(s):

Probiotic Properties ◽

Potential Use ◽

Bacillus Strains

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Dynamic Analysis of Prime Mover Combined Heat and Power Delivery to Chemical Processing

ASME 2011 Internal Combustion Engine Division Fall Technical Conference ◽

10.1115/icef2011-60169 ◽

2011 ◽

Author(s):

Michael E. Reed ◽

Diana K. Grauer

Keyword(s):

Chemical Process ◽

Power Input ◽

Combined Heat And Power ◽

Power Delivery ◽

Chemical Plants ◽

Prime Mover ◽

Engine Operation ◽

Unit Operations ◽

Aspen Custom Modeler ◽

The Impact

This paper presents a study of the impact of combined heat and power generation from prime mover engines on coupled chemical processes. Medium- and large-bore engines are frequently used in refineries and chemical plants to provide dedicated mechanical power to unit operations. Currently, the prime mover is included in the chemical process design as a single, steady-state power input. This design does not take into account, dynamic engine operation including load changes, start-up, shut-down, and malfunction, all of which have a direct and immediate impact on unit operations. The research team has developed a dynamic model of a “proof of concept” example chemical process in Aspen Custom Modeler to investigate the impact of dynamic operation and integration of a dedicated prime mover unit on an example chemical process.

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Exploring the relationship between exposure to technological and gastrointestinal stress and probiotic functional properties of lactobacilli and bifidobacteria

Canadian Journal of Microbiology ◽

10.1139/cjm-2016-0186 ◽

2016 ◽

Vol 62 (9) ◽

pp. 715-725 ◽

Cited By ~ 23

Author(s):

O.D. Amund

Keyword(s):

Functional Properties ◽

Health Benefits ◽

Stress Conditions ◽

Probiotic Properties ◽

Negative Effects ◽

Potential Health ◽

Colonization Potential ◽

Protective Strategies ◽

Processing And Storage ◽

The Impact

Strains of Lactobacillus and Bifidobacterium are considered probiotic because of their associated potential health benefits. Probiotics are commonly administered orally via incorporation into food products. Microorganisms for use as probiotics encounter stress conditions, which include acid, bile, osmotic, oxidative, heat and cold stresses. These can occur during processing and storage and during passage through the gastrointestinal tract, and can affect viability. Probiotic bacteria have to remain viable to confer any health benefits. Therefore, the ability to withstand technological and gastrointestinal stresses is crucial probiotic selection criteria. While the stress tolerance mechanisms of lactobacilli and bifidobacteria are largely understood, the impact of exposure to stressful conditions on the functional properties of surviving probiotic microorganisms is not clear. This review explores the potentially positive and negative relationships between exposure to stress conditions and probiotic functional properties, such as resistance to gastric acid and bile, adhesion and colonization potential, and tolerance to antibiotics. Protective strategies can be employed to combat negative effects of stress on functional properties. However, further research is needed to ascertain synergistic relationships between exposure to stress and probiotic properties.

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A pilot study on the effect of Lactobacillus casei Zhang on intestinal microbiota parameters in Chinese subjects of different age

Beneficial Microbes ◽

10.3920/bm2013.0047 ◽

2014 ◽

Vol 5 (3) ◽

pp. 295-304 ◽

Cited By ~ 25

Author(s):

L.Y. Kwok ◽

L. Wang ◽

J. Zhang ◽

Z. Guo ◽

H. Zhang

Keyword(s):

Lactobacillus Casei ◽

Age Groups ◽

Short Chain Fatty Acids ◽

Fermented Milk ◽

In Vitro Tests ◽

Probiotic Properties ◽

Lactobacillus Casei Zhang ◽

The Impact ◽

Chinese Subjects

Ageing of the population is an imminent global problem. Lactobacillus casei Zhang (LcZ) was isolated from Inner Mongolian fermented milk, koumiss. LcZ possesses numerous probiotic properties in in vitro tests and in animal models. However, it has never been tested in any human trial. In the current study, the impact of oral consumption of LcZ on different age groups was tested. Chinese subjects, including 10 young, 7 middle-aged and 7 elderly volunteers (with mean age of 24.3, 47.6 and 64.7, respectively), were recruited. Each subject took 10.6 log10 cfu LcZ daily for a continuous period of 28 days. Several parameters, including the amounts of LcZ and four selected groups of bacteria, change of bacterial diversity, short chain fatty acids (SCFA) and total bile acids (TBA), were monitored in faecal samples collected from the subjects before starting, during and after stopping oral LcZ consumption. The consumption of LcZ exhibited beneficial effects to the subjects by modulating faecal microbiota in a temporal manner with a prolonged elevation of SCFA and reduction of TBA. The potentially harmful Pseudomonas and Acinetobacter genera were suppressed by the probiotic administration. Furthermore, a moderately divergent response was observed in the indigenous gut populations of Bifidobacterium and Bacteroides fragilis group in different age subjects. Taken together, the current study has provided proof on the positive effect of probiotic consumption and crucial insights into the design and application of probiotic-based products to users of different age segments.

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