scholarly journals Evaluation of Antagonistic Bacillus and Biochar against Soybean Root Rot Caused by Fusarium oxysporum

2021 ◽  
Vol 26 (02) ◽  
pp. 287-293
Author(s):  
Fan Yang
Keyword(s):  
Root Rot ◽  
Fusarium Species ◽  
Dry Weight ◽  
Control Measures ◽  
Soybean Root ◽  
Soybean Seedlings ◽  
Yield And Quality ◽  
Soybean Plants ◽  
The Impact ◽  
Bacillus Strains

Soybean root rot is a worldwide soil-borne fungal disease threatening soybean production, causing huge losses in yield and quality of soybean. Fusarium species are well recognized as the important causal agent of Fusarium root rot. To screen the beneficial Bacillus strains with capability of suppressing soybean root rot and evaluate the impact of Bacillus combined with biochar against soybean root rot, a pot experiment was conducted with different treatments. In this study, as potential biological control measures, antagonistic Bacillus isolates and different types of biochar were added to soil separately and excellent antagonistic strains mixed with bamboo biochar were applied to the soil. The results showed that seven Bacillus strains promoted the growth of soybean seedlings and reduced root rot severity by 33 to 61%. Bacillus amylolique faciens NH2 was associated with the lowest incidence of soybean root rot, indicating its bio-control potential. The value of plant height, root length and plant dry weight of soybean in the sterilized soil mixed with biochar separately treatment were superior to those of soybean in the inoculated with pathogen treatment, especially the bamboo biochar treatment reduced soybean root rot caused by Fusarium significantly and which bio-control efficacy was 77.41%. The soybean plants shoot and root dry weights in the biochar mixed B. amylolique faciens NH2 or B. subtilis DBK treatments were increased by17.1, 10.7% and 19.51, 19.64%, respectively, which were significantly higher than those of the inoculated pathogen treatment. Compared to antagonistic strain or biochar individual treatments, the disease control efficiency on soybean root rot was up to 64.86% in NH2 strain mixed with bamboo biochar treatment, which reduced root rot severity significantly and showed a synergistic effect. These results suggest that antagonistic Bacillus strains mixed with biochar can be used as an effective alternative in managing soybean root rot. © 2021 Friends Science Publishers

scholarly journals The Impact of Road Light on Growth and Result of Soybean Plant

2020 ◽  
Vol 5 (2) ◽  
pp. 107-114
Author(s):  
Jajuk Herawati ◽  
I. Indarwati ◽  
Tatuk Tojibatus S. ◽  
Mochamad Thohiron ◽  
Heru Prasetyo
Keyword(s):  
Environmental Changes ◽  
Block Design ◽  
Price Volatility ◽  
Dry Weight ◽  
Growth And Yield ◽  
High Price ◽  
Soybean Plant ◽  
Street Lighting ◽  
Soybean Plants ◽  
The Impact

Until now, soybean is still one of the priority food commodities in Indonesia. in the agricultural revitalization program launched by the government in 2005, due to the high price volatility that did not rule out the possibility of shaking the Indonesian economy. Soybean plants can provide positive and negative responses to environmental changes growing above and in the soil.  This response can be known from phenotypic and physiological changes in plants. The environment on land which influences the growth of soybean plants mainly is the duration and intensity of irradiation, air temperature, CO2 content in the atmosphere. The study aims to determine the impact of street lighting on the growth and yield of soybean plants.  The study used a Randomized Block Design Method with 3 treatments,  and each treatment was repeated 9 times, so that it takes 27 treatment plots. J0= Distance of street lighting to soybean land (meters), J1: 50 meters, J2: 60 meters, and J3: 70 meters.  Observations were made one week after planting at 7-day intervals for growth parameters (plant height and number of leaves), while for the production parameters (Number of Content Pods/Plants, Dry Weight (DW) 100 Seeds, DW Seeds/Plots, and DW Seeds/Ha) are carried out after harvest. From the results of the study it can be concluded that there are real differences in the parameters of growth and production (number of filled pods, DW 100 seeds, DW seeds/plot, and DW seeds/ha), where the J3 treatment is capable of producing 2.89 tons/ha (an increase in dry weight of seeds/ha by 28.4%), compared to J1 2.24 tonnes/ha , although not significantly different from Treatment  J2

scholarly journals Impact of selected antagonistic fungi on Fusarium species – toxigenic cereal pathogens

2013 ◽  
Vol 43 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Delfina Popiel ◽  
Hanna Kwaśny ◽  
Jerzy Chełkowski ◽  
Łukasz Stępień ◽  
Magdalena Laskowska
Keyword(s):  
Fusarium Species ◽  
Fungal Species ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Fusarium Avenaceum ◽  
Dual Culture ◽  
Ear Blight ◽  
Yield And Quality ◽  
Significant Yield ◽  
The Impact

<em>Fusarium</em>-ear blight is a destructive disease in various cereal-growing regions and leads to significant yield and quality losses for farmers and to contamination of cereal grains with mycotoxins, mainly deoxynivalenol and derivatives, zearalenone and moniliformin. <em>Fusarium</em> pathogens grow well and produce significant inoculum on crop resiudues. Reduction of mycotoxins production and pathogen sporulation may be influenced by saprophytic fungi, exhibiting antagonistic effect. Dual culture bioassays were used to examine the impact of 92 isolates (belonging to 29 fungal species) against three toxigenic species, i.e. <em>Fusarium avenaceum</em> (Corda) Saccardo, <em>F. culmorum</em> (W.G.Smith) Saccardo and <em>F. graminearum</em> Schwabe. Both <em>F.culmorum</em> and <em>F. graminearum</em> isolates produce trichothecene mycotoxins and mycohormone zearalenone and are considered to be the most important cereal pathogens worldwide. Infection with those pathogens leads to accumulation of mycotoxins: deoxynivalenol (DON) and zearalenone (ZEA) in grains. <em>Fusarium avenaceum</em> isolates are producers of moniliformin (MON) and enniatins. Isolates of <em>Trichoderma</em> sp. were found to be the most effective ones to control the growth of examined <em>Fusarium</em> species. The response of <em>Fusarium</em> isolates to antagonistic activity of <em>Trichoderma</em> isolates varied and also the isolates of <em>Trichoderma</em> differed in their antagonistic activity against <em>Fusarium</em> isolates. The production of MON by two isolates of F. avenaceum in dual culture on rice was reduced by 95% to 100% by <em>T. atroviride</em> isolate AN 35. The same antagonist reduced the amount of moniliformin from 100 μg/g to 6.5 μg/g when inoculated to rice culture contaminated with MON, which suggests the possible decomposition of this mycotoxin.

scholarly journals Changes in the Density and Composition of Rhizosphere Pathogenic Fusarium and Beneficial Trichoderma Contributing to Reduced Root Rot of Intercropped Soybean

2021 ◽  
Author(s):  
Xiaoli Chang ◽  
Huiting Xu ◽  
Li Yan ◽  
Dan Zhu ◽  
Wei Wang ◽  
...  
Keyword(s):  
Root Rot ◽  
Cell Walls ◽  
Rhizosphere Soil ◽  
Fusarium Species ◽  
Inhibitory Effect ◽  
Trichoderma Species ◽  
Soybean Root ◽  
The Difference ◽  
Rhizosphere Microbial Community

Abstract BackgroundThe dynamic of soil-borne disease is closely related to the rhizosphere microbial communities. Maize-soybean intercropping can suppress soybean root rot as compared to monoculture. However, it is still unknown whether rhizosphere microbial community participates in the regulation of intercropped soybean root rot.MethodsIn this study, the difference of rhizosphere Fusarium and Trichoderma community was compared between healthy or root-rotted soybean rhizosphere soil from soybean monoculture and maize-soybean intercropping, and the inhibitory effect of potential biocontrol Trichoderma against pathogenic Fusarium were examined.ResultsThe abundance of rhizosphere Fusarium was remarkably different between intercropping and monoculture, while Trichoderma was largely accumulated in healthy rhizosphere soil of intercropping rather than monoculture. Four rhizosphere Fusarium species identified were all pathogenic to soybean but displayed distinct composition and isolation proportion in the corresponding soil types. As the dominant and most aggressive species, F. oxysporum was more frequently isolated in diseased soil of monoculture. Furthermore, of three Trichoderma species identified, T. harzianum dramatically increased in the rhizosphere of intercropping rather than monoculture as compared to T. virens and T. afroharzianum. For in-vitro antagonism test, Trichoderma strains had antagonistic effects on F. oxysporum with the percentage of mycelial inhibition ranging of 50.59%-92.94%, and they displayed good mycoparasitic abilities against F. oxysporum through coiling around and entering into the hyphae, expanding along cell-cell lumen and even dissolving cell walls of target fungus.ConclusionThese results indicate maize-soybean intercropping significantly increase the density and composition proportion of beneficial Trichoderma to antagonist the pathogenic Fusarium species, thus contributing to the suppression of soybean root rot under intercropping.

Noncompetitive Effects of Johnsongrass (Sorghum halepense) on Soybeans (Glycine max)

Weed Science ◽  
1982 ◽  
Vol 30 (6) ◽  
pp. 589-593 ◽  
Author(s):  
Petros C. Lolas ◽  
Harold D. Coble
Keyword(s):  
North Carolina ◽  
Glycine Max ◽  
Dry Weight ◽  
Soil Samples ◽  
Sorghum Halepense ◽  
Nutrient Concentrations ◽  
Soybean Seedling ◽  
Soybean Seedlings ◽  
Soybean Plants ◽  
Time Of Year

Studies were conducted in North Carolina to determine if johnsongrass [Sorghum halepense(L.) Pers.] rhizomes in the soil exude or produce substances that can affect soybean [Glycine max(L.) Merr.] seedling growth. Fresh and dry weights of soybean seedlings were dependent on the concentration of rhizomes present in the soil before soil samples were used for soybean growth, and on the time of year when soil samples were collected. In studies on rhizome residues, soybean seedling dry weight decreased as percent of dry, decayed rhizomes in the soil increased. For each rhizome concentration, soybean growth inhibition decreased as decay time increased. Fresh and dry weights of soybean plants were reduced by diluted fresh rhizome extract used for irrigation once a week for 3 weeks. Since no rhizomes were present in most of the soils during soybean growth and since nutrient concentrations, organic matter, and pH were not different among the soils used, it is suggested that johnsongrass rhizomes living or decaying in the soil exude, contain, or produce substances that exhibit allelopathic characteristics to soybean growth.

Fusarium root rot complex in soybean: Molecular characterization, trichothecene formation and cross-pathogenicity.

2021 ◽  
Author(s):  
Mohamed Hafez ◽  
Ahmed Abdelmagid ◽  
Reem Aboukhaddour ◽  
Lorne R. Adam ◽  
Fouad Daayf
Keyword(s):  
Root Rot ◽  
Fusarium Species ◽  
Molecular Diagnostic ◽  
Soybean Seeds ◽  
Head Blight ◽  
Fusarium Spp ◽  
Fusarium Root Rot ◽  
Yield And Quality ◽  
Fusarium Pathogens ◽  
First Time

Soybean is threatened by many pathogens that negatively affect this crop's yield and quality, e.g., different Fusarium species that cause wilting and root rot diseases. Fusarium root rot (FRR) in soybean can be caused by F. graminearum and other Fusarium spp. that are associated with Fusarium head blight (FHB) in cereals. Therefore, it was important to enquire whether Fusarium pathogens from soybean can cause disease in wheat, and vice versa. Here, we investigated the Fusarium root rot complex in Manitoba (Canada) from symptomatic plants, using both culture- and molecular-based methods. We developed a molecular diagnostic toolkit to detect and differentiate between several Fusarium spp. involved in FHB and FRR, then we evaluated cross-pathogenicity of selected Fusarium isolates collected from soybean and wheat, and the results indicate that isolates recovered from one host can infect the other host. Trichothecene production by selected Fusarium spp. was also analyzed chemically using LC-MS in both soybean (root) and wheat (spike) tissues. Trichothecenes were also analyzed in soybean seeds from plants with FRR to check the potentiality of trichothecene translocation from infected roots to the seeds. All of the tested Fusarium isolates were capable of producing trichothecenes in wheat spikes and soybean roots, but no trichothecenes were detected in soybean seeds. This study provided evidence, for the first time, that trichothecenes were produced by several Fusarium spp. (F. cerealis, F. culmorum and F. sporotrichioides) during FRR development in soybean.

scholarly journals Maize/Soybean Relay Strip Intercropping Reduces the Occurrence of Fusarium Root Rot and Changes the Diversity of the Pathogenic Fusarium Species

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 211
Author(s):  
Xiaoli Chang ◽  
Li Yan ◽  
Muhammd Naeem ◽  
Muhammad Ibrahim Khaskheli ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Root Rot ◽  
Species Complex ◽  
Crop Yields ◽  
Fusarium Species ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Soybean Root ◽  
Continuous Maize ◽  
Strip Intercropping

Fusarium species are the most detrimental pathogens of soybean root rot worldwide, causing large loss in soybean production. Maize/soybean relay strip intercropping has significant advantages on the increase of crop yields and efficient use of agricultural resources, but its effects on the occurrence and pathogen population of soybean root rot are rarely known. In this study, root rot was investigated in the fields of the continuous maize/soybean strip relay intercropping and soybean monoculture. Fusarium species were isolated from diseased soybean roots and identified based on sequence analysis of translation elongation factor 1α (EF-1α) and RNA polymerase II second largest subunit (RPB2), and the diversity and pathogenicity of these species were also analyzed. Our results showed that intercropping significantly decreased soybean root rot over monoculture. A more diverse Fusarium population including Fusarium solani species complex (FSSC), F. incarnatum-equiseti species complex (FIESC), F. oxysporum, F. fujikuroi, F. proliferatum and F. verticillioides, F. graminearum and F. asiaticum was identified from intercropping while FSSC, FIESC, F. oxysporum, F. commune, F. asiaticum and F. meridionale were found from monoculture. All Fusarium species caused soybean root infection but exhibited distinct aggressiveness. The most aggressive F. oxysporum was more frequently isolated in monoculture than intercropping. FSSC and FIESC were the dominant species complex and differed in their aggressiveness. Additionally, F. fujikuroi, F. proliferatum and F. verticillioides were specifically identified from intercropping with weak or middle aggressiveness. Except for F. graminearum, F. meridionale and F. asiaticum were firstly reported to cause soybean root rot in China. This study indicates maize/soybean relay strip intercropping can reduce soybean root rot, change the diversity and aggressiveness of Fusarium species, which provides an important reference for effective management of this disease.

Response of soybean cultivars to root rot caused by Fusarium species

2010 ◽  
Vol 90 (5) ◽  
pp. 767-776 ◽  
Author(s):  
J X Zhang ◽  
A G Xue ◽  
H J Zhang ◽  
A E Nagasawa ◽  
J T Tambong
Keyword(s):  
United States ◽  
Root Rot ◽  
Fusarium Species ◽  
Seedling Emergence ◽  
Dry Weight ◽  
The United States ◽  
Pathogenic Species ◽  
Fusarium Root Rot ◽  
Soybean Cultivars ◽  
Soybean Roots

Fusarium root rot complex is a major soybean disease in Canada and the United States. Since 2006, four Fusarium species, F. oxysporum Schlechtendahl emend. Snyder & Hansen, F. graminearum Schwabe, F. avenaceum (Corda: Fr.) Sacc., and F. tricinctum (Corda) Sacc., have frequently been isolated from soybean roots in eastern Ontario, Canada. The objective of the current study was to screen 57 soybean cultivars that are commercially available in Canada for resistance to these four Fusarium root rot pathogens under greenhouse conditions. Based on root rot severity and reductions in seedling emergence, plant height and root dry weight, F. avenaceum was the most pathogenic species, followed by F. graminearum. The pathogenicity of F. oxysporum on soybean cultivars was not significantly different from that of F. tricinctum, but was significantly lower than that of F. graminearum. In replicated experiments, six, nine, eleven and seven cultivars were consistently rated as the most resistant to F. avenaceum, F. graminearum, F. oxysporum and F. tricinctum, respectively. Cultivar Maple Amber was resistant to all four Fusarium species based on root rot severity, while cultivar Altona was resistant to F. avenaceum, F. oxysporum and F. tricinctum. Four cultivars, 9004, AC Harmony, Lanark and Maple Arrow, each showed resistance to two different Fusarium species.Key words: Soybean, Glycine max, Fusarium root rot, Fusarium oxysporum, F. graminearum, F. avenaceum, F. tricinctum

Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot

2013 ◽  
Vol 93 (2) ◽  
pp. 221-236 ◽  
Author(s):  
J. X. Zhang ◽  
A. G. Xue ◽  
E. R. Cober ◽  
M. J. Morrison ◽  
H. J. Zhang ◽  
...  
Keyword(s):  
Plant Height ◽  
Root Rot ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Field Resistance ◽  
Adult Plant ◽  
Cultivar Resistance ◽  
Soybean Root ◽  
Root Dry Weight ◽  
Seedling Roots

Zhang, J. X., Xue, A. G., Cober, E. R., Morrison, M. J., Zhang, H. J., Zhang, S. Z. and Gregorich, E. 2013. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot. Can. J. Plant Sci. 93: 221–236. Root rot complex, caused by Fusarium and Rhizoctonia species, is a major soybean disease in Canada. We isolated nine Fusarium and Rhizoctonia species including F. oxysporum (Fo), F. graminearum (Fg), F. solani (Fs), F. avenaceum (Fa), F. tricinctum (Ft), F. sporotrichioides (Fsp), F. equiseti (Fe), F. poae (Fp), and R. solani (Rs) from soybean roots in eastern Ontario, Canada. The isolation results indicated that Fo was the most prevalent species while Fa, Fsp, and Fp were the least frequent species in the soybean rhizosphere. Numbers of Fo, Fs, Fg, and Rs isolates recovered from adult plant roots were significantly greater than those from seedling roots (P<0.01). The Rs, Fg and Fsp isolates were significantly more abundant in the no-till field than in the tilled field (P<0.01). Based on the greenhouse assays, Rs, Fg, and Fa were the most pathogenic species, while Fe and Fsp were the least pathogenic to soybean. The field resistance evaluation, based on the root rot severity, identified 21, 17, 30, and 3 out of 70 cultivars among the most tolerant to Fg, Fo, Fs, and Rs, respectively. A few of the cultivars showed partial resistance to multiple species, based on root rot severity and reduction in their seedling emergence, plant height, and root dry weight, but no cultivar was found to partially resist all four species. There was no correlation (P>0.05) between root rot severity and the reduction in seedling emergence, plant height, or root dry weight.

scholarly journals The critical period of aluminum stress on soybean root growth

2020 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Danner Sagala ◽  
Eka Suzanna ◽  
Prihanani Prihanani
Keyword(s):  
Root Growth ◽  
Critical Threshold ◽  
Aluminum Stress ◽  
Considerable Effort ◽  
Aluminum Exposure ◽  
Soybean Root ◽  
Soybean Seedlings ◽  
Soybean Roots ◽  
Tidal Swamps ◽  
The Impact

Aluminum is prevalent in soils of tidal swamps. Soybean is known to be very sensitive to aluminum stress and so when tidal swamps are converted to soybean cropland, considerable effort and expense are required to overcome Al toxicity in soybean roots. It is therefore necessary to determine at what time in early development soybeans can best endure aluminum stress and identify aluminum-tolerant cultivars. This study was conducted by testing the impact of aluminum exposure on three soybean cultivars (Tanggamus, Karasumame, and M652) (relative to no-exposure controls) at four time periods at 10, 20, and 30 days after planting. No significant effect of aluminum on root growth in the first five days after exposure was observed, but the toxic effects became evident after soybeans had been exposed to aluminum for 10 days. Soybean seedlings that experienced aluminum stress earliest (at 10 days after planting) were more negatively impacted by Al exposure than seedlings exposed later (e.g., 30 days after planting). Root growths of the three cultivars we tested in this study were all detrimentally impacted by aluminum exposure. However, the M652 cultivar was the most sensitive to aluminum exposure. We conclude that the critical threshold period for soybean root growth to succumb to aluminum stress is within the first 30 days after planting, whereas the tolerance to aluminum stress occurs only during the first 10 days of exposure.

scholarly journals A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Tianjie Sun ◽  
Nan Ma ◽  
Caiqing Wang ◽  
Huifen Fan ◽  
Mengxuan Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Soil Salinization ◽  
Tolerance Mechanism ◽  
Salt Treatment ◽  
Soybean Seedlings ◽  
Yield And Quality ◽  
Sodium Hydrogen ◽  
Sodium Hydrogen Exchanger ◽  
Soybean Plants

Salt stress caused by soil salinization, is one of the main factors that reduce soybean yield and quality. A large number of genes have been found to be involved in the regulation of salt tolerance. In this study, we characterized a soybean sodium/hydrogen exchanger gene GmNHX5 and revealed its functional mechanism involved in the salt tolerance process in soybean. GmNHX5 responded to salt stress at the transcription level in the salt stress-tolerant soybean plants, but not significantly changed in the salt-sensitive ones. GmNHX5 was located in the Golgi apparatus, and distributed in new leaves and vascular, and was induced by salt treatment. Overexpression of GmNHX5 improved the salt tolerance of hairy roots induced by soybean cotyledons, while the opposite was observed when GmNHX5 was knockout by CRISPR/Cas9. Soybean seedlings overexpressing GmNHX5 also showed an increased expression of GmSOS1, GmSKOR, and GmHKT1, higher K+/Na+ ratio, and higher viability when exposed to salt stress. Our findings provide an effective candidate gene for the cultivation of salt-tolerant germplasm resources and new clues for further understanding of the salt-tolerance mechanism in plants.

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