Regulation of UCP3 expression and function in response to hypoxia and oxidative stress in mouse cardiomyocytes

Vascular endothelium plays a vital role in the organization and function of the blood vessel and maintains homeostasis of the circulatory system and normal arterial function. Functional disruption of the endothelium is recognized as the beginning event that triggers the development of consequent cardiovascular disease (CVD) including atherosclerosis and coronary heart disease. There is a growing data associating mercury exposure with endothelial dysfunction and higher risk of CVD. This review explores and evaluates the impact of mercury exposure on CVD and endothelial function, highlighting the interplay of nitric oxide and oxidative stress.

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Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis

Scientific Reports ◽

10.1038/s41598-019-48262-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 9

Author(s):

Somayeh Keshtkar ◽

Maryam Kaviani ◽

Zahra Jabbarpour ◽

Bita Geramizadeh ◽

Elahe Motevaseli ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Human Islets ◽

Induced Apoptosis ◽

And Function ◽

And Oxidative Stress

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Elevated protein carbonylation and oxidative stress do not affect protein structure and function in the long-living naked-mole rat: A proteomic approach

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2013.04.019 ◽

2013 ◽

Vol 434 (4) ◽

pp. 815-819 ◽

Cited By ~ 22

Author(s):

Eric M. De Waal ◽

Hanyu Liang ◽

Anson Pierce ◽

Ryan T. Hamilton ◽

Rochelle Buffenstein ◽

...

Keyword(s):

Oxidative Stress ◽

Protein Structure ◽

Structure And Function ◽

Protein Carbonylation ◽

Proteomic Approach ◽

Mole Rat ◽

Elevated Protein ◽

Naked Mole Rat ◽

And Function ◽

And Oxidative Stress

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Adverse Effects of Heat Stress on the Intestinal Integrity and Function of Pigs and the Mitigation Capacity of Dietary Antioxidants: A Review

Animals ◽

10.3390/ani11041135 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1135

Author(s):

Arth David Sol Valmoria Ortega ◽

Csaba Szabó

Keyword(s):

Oxidative Stress ◽

Heat Stress ◽

Adverse Effects ◽

Environmental Temperature ◽

Nutritional Intervention ◽

Intestinal Histology ◽

Dietary Antioxidants ◽

Intestinal Integrity ◽

And Function ◽

And Oxidative Stress

Heat stress (HS) significantly affects the performance of pigs by its induced stressors such as inflammation, hypoxia and oxidative stress (OS), which mightily strain the intestinal integrity and function of pigs. As heat stress progresses, several mechanisms in the intestinal epithelium involved in the absorption of nutrients and its protective functions are altered. Changes in these mechanisms are mainly driven by cellular oxidative stress, which promotes disruption of intestinal homeostasis, leading to intestinal permeability, emphasizing intestinal histology and morphology with little possibility of recovering even after exposure to HS. Identification and understanding of these altered mechanisms are crucial for providing appropriate intervention strategies. Therefore, it is this papers’ objective to review the important components for intestinal integrity that are negatively affected by HS and its induced stressors. With due consideration to the amelioration of such effects through nutritional intervention, this work will also look into the capability of dietary antioxidants in mitigating such adverse effects and maintaining the intestine’s integrity and function upon the pigs’ exposure to high environmental temperature.

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Characterization of Mitochondrial GSH Transporters and Their Role in RPE Protection

10.20944/preprints201807.0346.v1 ◽

2018 ◽

Author(s):

Mo Wang ◽

Ling-ing Lau ◽

Parameswaran G. Sreekumar ◽

Christine Spee ◽

Lin Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Age Related Macular Degeneration ◽

Ocular Tissues ◽

Age Related ◽

And Function ◽

And Oxidative Stress ◽

Pigment Epithelial Cells

Mitochondrial dysfunction and oxidative stress are thought to be relevant to the pathogenesis of age-related macular degeneration (AMD). Glutathione (GSH) homeostasis fulfills a number of important roles in mitochondria, such as maintenance of mitochondrial DNA and respiratory competency of cells. Although the transport of mitochondrial GSH (mGSH) is not fully understood, increasing evidence from non-ocular tissues suggests that OGC (2-oxoglutarate carrier, SLC25A11) and DIC (dicarboxylate carrier, SLC25A10) are involved in mGSH transport. However, whether OGC and DIC mediate the transfer of GSH into the mitochondria of retinal pigment epithelial cells (RPE) remains unknown. Thus, we investigated the expression, localization, and function of OGC and DIC in human RPE (hRPE) in relation to oxidative stress and GSH. Both OGC and DIC are expressed in hRPE and are localized in mitochondria. We also found a dose and time-dependent decrease of OGC and DIC expression under oxidative stress and increased expression in polarized RPE. Our data show that the downregulation of OGC and DIC resulted in increased apoptosis and mGSH depletion which can be overcome by co-treatment with GSH-MEE. These findings suggest that overexpression of OGC and DIC may be an effective strategy to decrease susceptibility to mitochondrial toxicants by elevation of mGSH.

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Left Ventricular Structure and Function in Long-Term Kidney Transplantation: The Influence of Glucose Metabolism and Oxidative Stress

Transplantation Proceedings ◽

10.1016/j.transproceed.2008.09.027 ◽

2008 ◽

Vol 40 (9) ◽

pp. 2912-2915 ◽

Cited By ~ 5

Author(s):

J.M. Osorio Moratalla ◽

C. Ferreyra Lanatta ◽

Y. Baca Morilla ◽

E. Romero Ramírez ◽

J.M. Moreno Ayuso ◽

...

Keyword(s):

Oxidative Stress ◽

Kidney Transplantation ◽

Glucose Metabolism ◽

Left Ventricular ◽

Structure And Function ◽

Left Ventricular Structure ◽

Ventricular Structure ◽

And Function ◽

And Oxidative Stress

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Nitric oxide and oxidative stress pathways do not contribute to sex differences in renal injury and function in Dahl SS/Jr rats

Physiological Reports ◽

10.14814/phy2.14440 ◽

2020 ◽

Vol 8 (13) ◽

Cited By ~ 1

Author(s):

Hannah R. Turbeville ◽

Ashley C. Johnson ◽

Michael R. Garrett ◽

Elena L. Dent ◽

Jennifer M. Sasser

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Sex Differences ◽

Renal Injury ◽

And Function ◽

And Oxidative Stress

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Irisin Rescues Blood-Brain Barrier Permeability following Traumatic Brain Injury and Contributes to the Neuroprotection of Exercise in Traumatic Brain Injury

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/1118981 ◽

2021 ◽

Vol 2021 ◽

pp. 1-18

Author(s):

Peipei Guo ◽

Zhao Jin ◽

Jin Wang ◽

Aming Sang ◽

Huisheng Wu

Keyword(s):

Oxidative Stress ◽

Traumatic Brain Injury ◽

Brain Injury ◽

Cytochrome C ◽

Brain Edema ◽

Blood Brain Barrier ◽

Mitochondrial Membrane ◽

Brain Barrier ◽

And Function ◽

And Oxidative Stress

Traumatic brain injury (TBI) has a high incidence, mortality, and morbidity all over the world. One important reason for its poor clinical prognosis is brain edema caused by blood-brain barrier (BBB) dysfunction after TBI. The mechanism may be related to the disorder of mitochondrial morphology and function of neurons in damaged brain tissue, the decrease of uncoupling protein 2 (UCP2) activity, and the increase of inflammatory reaction and oxidative stress. In this study, we aimed to investigate the effects of exogenous irisin on BBB dysfunction after TBI and its role in the neuroprotective effects of endurance exercise (EE) in mice. The concentrations of irisin in cerebrospinal fluid (CSF) and plasma of patients with mild to severe TBI were measured by ELISA. Then, male C57BL/6J mice and UCP2 knockout mice with C57BL/6J background were used to establish the TBI model. The BBB structure and permeability were examined by transmission electron microscopy and Evans blue extravasation, respectively. The protein expressions of irisin, occludin, claudin-5, zonula occludens-1 (ZO-1), nuclear factor E2-related factor 2(Nrf2), quinine oxidoreductase (NQO-1), hemeoxygenase-1 (HO-1), cytochrome C (Cyt-C), cytochrome C oxidase (COX IV), BCL2-associated X protein (Bax), cleaved caspase-3, and UCP2 were detected by western blot. The production of reactive oxygen species (ROS) was evaluated by the dihydroethidium (DHE) staining. The levels of inflammatory factors were detected by ELISA. In this study, we found that the CSF irisin levels were positively correlated with the severity of disease in patients with TBI and both EE and exogenous irisin could reduce BBB damage in a mouse model of TBI. In addition, we used UCP2−/− mice and further found that irisin could improve the dysfunction of BBB after TBI by promoting the expression of UCP2 on the mitochondrial membrane of neurons, reducing the damage of mitochondrial structure and function, thus alleviating the inflammatory response and oxidative stress. In conclusion, the results of this study suggested that irisin might alleviate brain edema after TBI by promoting the expression of UCP2 on the mitochondrial membrane of neurons and contribute to the neuroprotection of EE against TBI.

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Tight Junction Proteins and Oxidative Stress in Heavy Metals-Induced Nephrotoxicity

BioMed Research International ◽

10.1155/2013/730789 ◽

2013 ◽

Vol 2013 ◽

pp. 1-14 ◽

Cited By ~ 37

Author(s):

José L. Reyes ◽

Eduardo Molina-Jijón ◽

Rafael Rodríguez-Muñoz ◽

Pablo Bautista-García ◽

Yazmin Debray-García ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metals ◽

Intercellular Junctions ◽

Main Site ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid ◽

And Function ◽

And Oxidative Stress ◽

Relevant Component ◽

Junction Proteins

Kidney is a target organ for heavy metals. They accumulate in several segments of the nephron and cause profound alterations in morphology and function. Acute intoxication frequently causes acute renal failure. The effects of chronic exposure have not been fully disclosed. In recent years increasing awareness of the consequences of their presence in the kidney has evolved. In this review we focus on the alterations induced by heavy metals on the intercellular junctions of the kidney. We describe that in addition to the proximal tubule, which has been recognized as the main site of accumulation and injury, other segments of the nephron, such as glomeruli, vessels, and distal nephron, show also deleterious effects. We also emphasize the participation of oxidative stress as a relevant component of the renal damage induced by heavy metals and the beneficial effect that some antioxidant drugs, such as vitamin A (all-trans-retinoic acid) and vitamin E (α-tocopherol), depict on the morphological and functional alterations induced by heavy metals.

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