The attenuation of antibiotic resistant non-albicans Candida species, cytotoxicity, anti-inflammatory effects and phytochemical profiles of five Vachellia species by FTIR and UHPLC–Q/Orbitrap/MS

Abstract Phytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and β-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and α-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 μg/mL, whereas, CAD showed the best inhibition of α-glucosidase with an IC50 value of 396.40 μg/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.

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Combined Antibacterial and Anti-Inflammatory Activity of a Cationic Disubstituted Dexamethasone-Spermine Conjugate

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01682-09 ◽

2010 ◽

Vol 54 (6) ◽

pp. 2525-2533 ◽

Cited By ~ 10

Author(s):

Robert Bucki ◽

Katarzyna Leszczyńska ◽

Fitzroy J. Byfield ◽

David E. Fein ◽

Esther Won ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Bacterial Infections ◽

Glucocorticoid Receptors ◽

Fluorescent Protein ◽

Lipoteichoic Acid ◽

Bacterial Strains ◽

Bacterial Killing ◽

Antibiotic Resistant ◽

Anti Inflammatory ◽

Green Fluorescent

ABSTRACT The rising number of antibiotic-resistant bacterial strains represents an emerging health problem that has motivated efforts to develop new antibacterial agents. Endogenous cationic antibacterial peptides (CAPs) that are produced in tissues exposed to the external environment are one model for the design of novel antibacterial compounds. Here, we report evidence that disubstituted dexamethasone-spermine (D2S), a cationic corticosteroid derivative initially identified as a by-product of synthesis of dexamethasone-spermine (DS) for the purpose of improving cellular gene delivery, functions as an antibacterial peptide-mimicking molecule. This moiety exhibits bacterial killing activity against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa present in cystic fibrosis (CF) sputa, and Pseudomonas aeruginosa biofilm. Although compromised in the presence of plasma, D2S antibacterial activity resists the proteolytic activity of pepsin and is maintained in ascites, cerebrospinal fluid, saliva, and bronchoalveolar lavage (BAL) fluid. D2S also enhances S. aureus susceptibility to antibiotics, such as amoxicillin (AMC), tetracycline (T), and amikacin (AN). Inhibition of interleukin-6 (IL-6) and IL-8 release from lipopolysaccharide (LPS)- or lipoteichoic acid (LTA)-treated neutrophils in the presence of D2S suggests that this molecule might also prevent systemic inflammation caused by bacterial wall products. D2S-mediated translocation of green fluorescent protein (GFP)-labeled glucocorticoid receptor (GR) in bovine aorta endothelial cells (BAECs) suggests that some of its anti-inflammatory activities involve engagement of glucocorticoid receptors. The combined antibacterial and anti-inflammatory activities of D2S suggest its potential as an alternative to natural CAPs in the prevention and treatment of some bacterial infections.

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UPLC-Q-Exactive Orbitrap/MS-Based Lipidomics Approach To Characterize Lipid Extracts from Bee Pollen and Their in Vitro Anti-Inflammatory Properties

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.7b02285 ◽

2017 ◽

Vol 65 (32) ◽

pp. 6848-6860 ◽

Cited By ~ 19

Author(s):

Qiangqiang Li ◽

Xinwen Liang ◽

Liang Zhao ◽

Zhongyin Zhang ◽

Xiaofeng Xue ◽

...

Keyword(s):

Bee Pollen ◽

Q Exactive ◽

Anti Inflammatory ◽

Orbitrap Ms

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In-vitro anti-inflammatory activity, free radical (DPPH) scavenging, and ferric reducing ability (FRAP) of Sargassum cristaefolium lipid-soluble fraction and putative identification of bioactive compounds using UHPLC-ESI-ORBITRAP-MS/MS

Food Research International ◽

10.1016/j.foodres.2020.109702 ◽

2020 ◽

Vol 137 ◽

pp. 109702

Author(s):

Saraswati ◽

Puspo Edi Giriwono ◽

Diah Iskandriati ◽

Chin Ping Tan ◽

Nuri Andarwulan

Keyword(s):

Free Radical ◽

Bioactive Compounds ◽

Soluble Fraction ◽

Inflammatory Activity ◽

Reducing Ability ◽

Anti Inflammatory ◽

Dpph Scavenging ◽

Orbitrap Ms

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Caralluma europaea (Guss.) N.E.Br.: Anti-Inflammatory, Antifungal, and Antibacterial Activities against Nosocomial Antibiotic-Resistant Microbes of Chemically Characterized Fractions

Molecules ◽

10.3390/molecules26030636 ◽

2021 ◽

Vol 26 (3) ◽

pp. 636

Author(s):

Fatima Ez-Zahra Amrati ◽

Mohammed Bourhia ◽

Hamza Saghrouchni ◽

Meryem Slighoua ◽

Andriy Grafov ◽

...

Keyword(s):

Bioactive Compounds ◽

Chemical Characterization ◽

Antibacterial Activities ◽

Antibiotic Resistant ◽

Traditional Medicines ◽

Gram Positive Bacteria ◽

Antibacterial And Antifungal Activities ◽

The Family ◽

Anti Inflammatory ◽

Antibacterial And Antifungal

Caralluma europaea (Guss.) N.E.Br.: (C. europaea) is a wild medicinal plant belonging to the family Apocynaceae. It is commonly used in traditional medicines for treating several diseases. The present work aims to evaluate the anti-inflammatory, antibacterial, and antifungal potentials of C. europaea fractions including hydro ethanol (ET CE), n-butanol (But CE), and polyphenol (Poly CE). The chemical composition of hydroethanol, n-butanol, and polyphenol-rich fractions from C. europaea were determined using GC-MS after silylation. The anti-inflammatory effect of hydroethanol, n-butanol, and polyphenol-rich fractions was studied by carrageenan-induced paw edema. Antibacterial and antifungal activities of hydroethanol, n-butanol, and polyphenol-rich fractions against Gram-positive bacteria, Gram-negative bacteria, and yeasts were assessed using the disc diffusion and micro-dilution assays. The findings of the chemical characterization affirmed the presence of interesting bioactive compounds in C. europaea fractions. The polyphenol-rich fraction was the best inhibitor of edema by75.68% after 6 h of treatment. The hydroethanol fraction was the most active against both bacteria and yeasts. This study contributes to society as it provides potential bioactive compounds in C. europaea extract, which may help in fighting nosocomial antibiotic-resistant microbes.

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Pemanfaatan tanaman obat untuk pengendalian penyakit dalam usaha budidaya

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.5.2.2017.24260 ◽

2017 ◽

Vol 5 (2) ◽

Author(s):

Shifa A. Schram

Keyword(s):

Medicinal Plants ◽

Tropical Country ◽

Antibiotic Resistant ◽

Fish Diseases ◽

Betel Leaf ◽

Surrounding Environment ◽

Anti Inflammatory ◽

Bacteria And Fungi ◽

Guava Leaf ◽

Antibacterial And Antifungal

Production of global and national aquaculture often fails due to outbreaks of fish diseases caused by viruses, bacteria, fungi and parasites. Moreover, as a tropical country, Indonesia has a climate that strongly supports the development of pathogens. The occurrence of disease outbreak is basically as a result of disturbance of the balance interaction between fish, the unfavorable environment and pathogens. Medicinal plants are rich of antimicrobial compounds such as naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids, which are effective for inhibiting the growth of bacteria and fungi. Oleoresin compounds, i.e. essential oils that act as antibacterial and antifungal are also present in medicinal plants. Medicinal plants also serve to stop the bleeding, because there are anti-inflammatory compounds (anti-inflammatory). This compound is found in several medicinal plants that have been tested to cope with fish diseases such as rose balsam, guava leaf, garlic, betel leaf and ginger. Some of the advantages of using traditional medicinal plants are relatively safe, easy to obtain, cheap, not resistant, and relatively harmless to the surrounding environment and do not create antibiotic-resistant pathogens. Thus, it is more adviseable to use medicinal plants in controlling fish diseases. Keywords: medicinal plants, aquaculture, aquaculture, fish diseases

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Lipid Profiles of the Heads of Four Shrimp Species by UPLC–Q–Exactive Orbitrap/MS and Their Cardiovascular Activities

Molecules ◽

10.3390/molecules27020350 ◽

2022 ◽

Vol 27 (2) ◽

pp. 350

Author(s):

Yongqiang Zhu ◽

Peihai Li ◽

Ronghua Meng ◽

Xiaobin Li ◽

Yuezi Qiu ◽

...

Keyword(s):

Fenneropenaeus Chinensis ◽

Structural Function ◽

Key Factors ◽

Zebrafish Model ◽

Penaeus Japonicus ◽

Shrimp Head ◽

Q Exactive ◽

Anti Inflammatory ◽

Different Sources ◽

Orbitrap Ms

Lipids are key factors in nutrition, structural function, metabolic features, and other biological functions. In this study, the lipids from the heads of four species of shrimp (Fenneropenaeus chinensis (FC), Penaeus japonicus (PJ), Penaeus vannamei (PV), and Procambarus clarkia (PCC)) were compared and characterized based on UPLC–Q–Exactive Orbitrap/MS. We compared the differences in lipid composition of four kinds of shrimp head using multivariate analysis. In addition, a zebrafish model was used to evaluate pro-angiogenic, anti-inflammatory, anti-thrombotic, and cardioprotective activities of the shrimp head lipids. The lipids from the four kinds of shrimp head had different degrees of pro-angiogenic activities, and the activities of PCC and PJ shrimp lipids were more significant than those of the other two species. Four lipid groups displayed strong anti-inflammatory activities. For antithrombotic activity, only PCC (25 μg/mL) and PV (100 μg/mL) groups showed obvious activity. In terms of cardioprotective activity, the four kinds of lipid groups significantly increased the zebrafish heart rhythms. The heart distances were shortened, except for those of the FC (100 μg/mL) and PJ (25 μg/mL) groups. Our comprehensive lipidomics analysis and bioactivity study of lipids from different sources could provide a basis for the better utilization of shrimp.

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