A comparative analysis of antibacterial activity, dynamics, and effects of silver ions and silver nanoparticles against four bacterial strains

2017 ◽  
Vol 123 ◽  
pp. 304-310 ◽  
Author(s):  
Wen-Ru Li ◽  
Ting-Li Sun ◽  
Shao-Lu Zhou ◽  
Yong-Kai Ma ◽  
Qing-Shan Shi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Comparative Analysis ◽  
Silver Ions ◽  
Bacterial Strains ◽  
Activity Dynamics

scholarly journals COUROUPITA GUIANENSIS LEAF CALLUS EXTRACT MEDIATED SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES.

2017 ◽  
Vol 10 (5) ◽  
pp. 126
Author(s):  
Jsr Murthy ◽  
Venkata Kumar T ◽  
Narayana Rao V
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cell Surface ◽  
Size Variation ◽  
Silver Ions ◽  
Bacterial Strains ◽  
Couroupita Guianensis ◽  
Spectral Peaks ◽  
Characteristic Maximum

Objective: Synthesis of varied sized and morphologically distinct silver nanoparticles (AgNPs) using callus/callus extract, and their promising antibacterial and cytotoxicity was reported from very few plant systems. Here, we investigated silver nanoparticle synthetic potential of Couroupita guianensis leaf callus extract and their antibacterial activity.Methods: synthesis of callus mediated silver nanoparticles and characterisation of physical, chemical, and antibacterial activity of AgNPs.Results: Callus extract rapidly reduced silver ions and stabilized nanoparticles have displayed characteristic maximum UV absorbance at 410 nm. SEM and AFM images revealed their spherical morphology and size variation, which is ranged from 30.38 nm to 88.32 nm and were in small aggregates. Capping of AgNPs by the phenolic compounds and proteins revealed from FTIR spectral peaks. Silver nanoparticles displayed significantly high antimicrobial activity against both Gram positive and negative bacterial strains compared to silver ions and gentamicin. This enhanced antimicrobial activity of AgNPs may due their small size leading to efficient molecular contact with cell surface, and uptake and interaction with vital biomolecules.Conclusions: Stable AgNPs were synthesized through reduction and capping of silver ions by polyphenols and proteins present in callus extract. Theenhanced antimicrobial activity of AgNPs may due to their small size leading to efficient molecular contact with the cell surface, penetration, andinteraction, and inactivation of vital biomolecules.KEY WORDS: Couroupita guianensis, callus extract, Silver nanoparticles (AgNPs), antibacterial activity

scholarly journals SILVER NANOPARTICLES FROM TRIANTHEMA PORTULACASTRUM: GREEN SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND ANTICANCER PROPERTIES

2017 ◽  
Vol 10 (3) ◽  
pp. 308
Author(s):  
Gowri Shankar Krishnan ◽  
Namrata Pradhan ◽  
Masilamani K ◽  
Albin T Fleming
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Synthesis Method ◽  
Silver Ions ◽  
Bacterial Strains ◽  
X Ray ◽  
Nih 3T3 ◽  
Dose Dependent

ABSTRACTObjective: In this study, silver nanoparticles (SNPs) were synthesized using an aqueous extract of Trainthema portulacastrum and silver ions (Ag+)which have been proven against certain pathogenic bacterial strains and hepatocellular carcinoma (HepG2) cell line.Methods: The bio fabricated nanoparticles were confirmed by surface plasmon resonance which were characterized by biophysical measuresutilizing the ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray, and transmission electron microscope(TEM), Fourier transform infrared spectroscopy, particle size analyzer, and X-ray diffraction. Antibacterial efficacy against Enterobacter aerogens,Proteus mirabilis, Escherichia coli, Staphylococcus epidermis, and Bacillus subtilis. The effect of SNPs tested against HepG2 and NIH/3T3 cell lineexhibits a dose-dependent toxicity.Results and Conclusion: The SEM and TEM images confirmed the presence of spherical and hexagonal shape (0.3-4 μm) of nanocrystalline particleswith the size range of 11.5-29.2 nm. The average particles size of SNPs is 190.3±17.0 nm. Antibacterial activity was carried out by agar well diffusionmethod against different pathogenic bacteria of which B. subtilis showed a significant zone of inhibition 8.66 mm and 12.0 mm for aqueous plantextract and synthesized SNPs. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. In case of HepG2, thecell viability was decreased to 50% (IC50) at the concentration of 173.8±0.84 μg/mL. From the results, it can be concluded that the SNPs fabricatedusing green synthesis method will be a promising candidate in the biomedical field, due to its high bioactive properties.Keywords: Silver nanoparticles, Trainthema portulacastrum, Antibacterial activity, Cytotoxic activity.

scholarly journals Biosynthesis of Silver Nanoparticles through Biomass of Fungus Aspergillusniger and their Antibacterial Potential

2020 ◽  
Vol 6 (6) ◽  
pp. 37-42
Author(s):  
Pushpendra Pratap Singh ◽  
Sarika Chaturvedi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Ions ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Extracellular Biosynthesis ◽  
Self Assembling ◽  
Antibiotic Drugs ◽  
The Fourier Transform ◽  
Antibacterial Potential

Extracellular biosynthesis of silver nanoparticles by fungus Aspergillus niger which was isolated from waste water is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-Vis spectrum, showing the absorbance between 260 to 400 nm. The nanoparticles characterized by Scanning Electron Microscopy exhibited silver nanoparticles with diameter of 25nm to 75nm. Energy Dispersive X-ray analysis reveals strong signals in the silver region and confirms the formation of the silver nanoparticles. The Fourier Transform Infrared Spectroscopy study confirmed that the A. niger mycomass has the ability to perform both reduction and capping functions on the silver nanoparticles. Compound Microscopy confirms the self-assembling property of silver nanoparticles. The silver nanoparticles showed remarkable antibacterial activity against Lactobacillus plantarum, Lactobacillus delbrueckii and Bacillus subtilis bacterial strains. Reduction of silver ions is an extracellular and rapid process; this information may lead to the development of easy protocols for biosynthesis of the silver nanoparticles. Antibacterial activity of silver nanoparticles is important for the development of effective antibacterial agent against those bacteria who are showing resistance against antibiotic drugs which are available in market.

scholarly journals Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

2019 ◽  
Vol 31 (12) ◽  
pp. 2804-2810
Author(s):  
Anti Kolonial Prodjosantoso ◽  
Oktanio Sigit Prawoko ◽  
Maximus Pranjoto Utomo ◽  
Lis Permana Sari
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Silver Ions ◽  
Reduction Reaction ◽  
Face Centered Cubic ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

A COMPARATIVE ANALYSIS OF VARIOUS SPECIES OF ASPERGILLUS MEDIATED SILVER NANOPARTICLES SYNTHESIS AND ITS ANTIBACTERIAL ACTIVITY

2014 ◽  
Vol 3 (1) ◽  
pp. 52-56
Author(s):  
N Prabhu ◽  
P Revathi ◽  
Jeyaseelan T Senthinath ◽  
Robert Asirvatham Alwin ◽  
Pushpa Innocent D Joseph
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Comparative Analysis ◽  
Nanoparticles Synthesis

scholarly journals A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Siraj Shaik ◽  
Madhusudana Rao Kummara ◽  
Sudhakar Poluru ◽  
Chandrababu Allu ◽  
Jaffer Mohiddin Gooty ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Silver Ions ◽  
Inhibition Zone ◽  
Zone Method ◽  
Green Approach ◽  
Tridax Procumbens ◽  
Poly Acrylamide

A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox polymerization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag+) into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications.

scholarly journals Preparation And Characterization of Sodium Alginate/Acrylic Acid Composite Hydrogels Conjugated To Silver Nanoparticles As An Antibiotic Delivery System

2021 ◽  
Author(s):  
Parvaneh Mohamadinia ◽  
Navideh Anarjan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Acrylic Acid ◽  
Sodium Alginate ◽  
Release Kinetics ◽  
Silver Ions ◽  
Composite Hydrogels ◽  
Gel Fraction ◽  
Good Biocompatibility ◽  
The Impact

Abstract Hydrogels are specific groups of polymers that are highly swellable in aqueous solutions, despite their water-in-soluble structures. Thus, they are promising drug delivery systems attributable to their unique characteristics such as high hydrophilicity, high controllability, facile production routines and, good biocompatibility. The aim of this research was the preparation of sodium alginate/acrylic acid composite hydrogels conjugated to silver nanoparticles to deliver the cephalexin as a model antibiotic compound. The reduction of silver ions into silver nanoparticles as well as the stabilization of created nanoparticles ensued simultaneously with hydrogel backbone formulation during microwave irradiation and monomer cross-linking processes. The impact of acrylic acid and silver ions concentrations and also the radiation time of microwave were then investigated on the main characteristics of hydrogels, namely, swelling ratio, gel fraction, cephalexin load and, antibacterial activity. The results indicated that the hydrogels’ characteristics could be significantly predicted by studied all independent parameters, through various second-order polynomial models. The multiple optimization analysis suggested that the prepared hydrogels using 7.8 g acrylic acid and 1.5 g silver nitrate and 1 min microwave radiation could give the best hydrogels with the highest swelling degree, gel fraction, cephalexin absorption and, antibacterial activity. The morphology and either absorption or release kinetics of cephalexin by/from the optimum prepared hydrogels were also investigated. No significant differences between the experimental and predicted data was confirmed the suitability of the suggested models.

Phytochemical Synthesis of Silver Nanoparticles Using Anthemis Nobilis Extract and Its Antibacterial Activity

2020 ◽  
Vol 234 (3) ◽  
pp. 531-540
Author(s):  
Saba Ghamipoor ◽  
Faeze Fayyazi ◽  
Saeed Bahadorikhalili
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Silver Ions ◽  
Good Alternative ◽  
Ft Ir ◽  
Average Size ◽  
Physical And Chemical

AbstractIn this work, green synthesis of silver nanoparticles is described by phytochemical reducing silver nitrate aqueous solution using Anthemis nobilis. For this purpose, Anthemis nobilis extract was used for the synthesis of silver nanoparticles as both surfactant and reducing agent. Green synthesis method is a good alternative to physical and chemical methods, since it is fast, simple, environmentally-friendly and economic. The produced nanoparticles are identified using FE-SEM, EDX, and FT-IR and Uv/Vis techniques. Formation of silver nanoparticles is verified in 430–420 nm range. Reduction of silver ions by hydroxyl functional group is also confirmed by FT-IR device. EDX device confirms the presence of a peak for Ag element without any impurity peak. Silver nanoparticles are identified by FE-SEM device and found to have average size between 17 and 42 nm. Also, the antibacterial activity of the synthesized nanoparticles is compared with that of staphyloccusaureus and pseudomonasa aeruginosa and the maximum inhibitory activity against the bacteria is obtained using 1 mM nitrate solution.

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