Preparation And Characterization of Sodium Alginate/Acrylic Acid Composite Hydrogels Conjugated To Silver Nanoparticles As An Antibiotic Delivery System

Mapping Intimacies ◽

10.21203/rs.3.rs-429387/v1 ◽

2021 ◽

Author(s):

Parvaneh Mohamadinia ◽

Navideh Anarjan

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Acrylic Acid ◽

Sodium Alginate ◽

Release Kinetics ◽

Silver Ions ◽

Composite Hydrogels ◽

Gel Fraction ◽

Good Biocompatibility ◽

The Impact

Abstract Hydrogels are specific groups of polymers that are highly swellable in aqueous solutions, despite their water-in-soluble structures. Thus, they are promising drug delivery systems attributable to their unique characteristics such as high hydrophilicity, high controllability, facile production routines and, good biocompatibility. The aim of this research was the preparation of sodium alginate/acrylic acid composite hydrogels conjugated to silver nanoparticles to deliver the cephalexin as a model antibiotic compound. The reduction of silver ions into silver nanoparticles as well as the stabilization of created nanoparticles ensued simultaneously with hydrogel backbone formulation during microwave irradiation and monomer cross-linking processes. The impact of acrylic acid and silver ions concentrations and also the radiation time of microwave were then investigated on the main characteristics of hydrogels, namely, swelling ratio, gel fraction, cephalexin load and, antibacterial activity. The results indicated that the hydrogels’ characteristics could be significantly predicted by studied all independent parameters, through various second-order polynomial models. The multiple optimization analysis suggested that the prepared hydrogels using 7.8 g acrylic acid and 1.5 g silver nitrate and 1 min microwave radiation could give the best hydrogels with the highest swelling degree, gel fraction, cephalexin absorption and, antibacterial activity. The morphology and either absorption or release kinetics of cephalexin by/from the optimum prepared hydrogels were also investigated. No significant differences between the experimental and predicted data was confirmed the suitability of the suggested models.

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Preparation and characterization of sodium alginate/acrylic acid composite hydrogels conjugated to silver nanoparticles as an antibiotic delivery system

Green Processing and Synthesis ◽

10.1515/gps-2021-0081 ◽

2021 ◽

Vol 10 (1) ◽

pp. 860-873

Author(s):

Parvaneh Mohamadinia ◽

Navideh Anarjan ◽

Hoda Jafarizadeh-Malmiri

Keyword(s):

Silver Nanoparticles ◽

Acrylic Acid ◽

Sodium Alginate ◽

Release Kinetics ◽

Silver Ions ◽

Optimization Analysis ◽

Composite Hydrogels ◽

The Impact ◽

Kinetics Of

Abstract Hydrogels are promising drug delivery systems attributable to their unique characteristics such as high hydrophilicity, controllability, biocompatibility, and facile production routines. The aim of this research was the preparation of sodium alginate/acrylic acid (AAc) composite hydrogels conjugated to silver nanoparticles to deliver the cephalexin as a model antibiotic compound. The reduction of silver ions into silver nanoparticles as well as the stabilization of created nanoparticles ensued simultaneously with hydrogel backbone formulation during microwave irradiation and monomer cross-linking processes. The impact of AAc and silver ion concentrations and the radiation time of microwave were then investigated on the main characteristics of hydrogels. The results indicated that the hydrogels’ characteristics could be significantly predicted by studying all independent parameters through various second-order polynomial models. The multiple optimization analysis suggested that the prepared hydrogels using 7.8 g AAc and 1.5 g silver nitrate and 1 min microwave radiation could give the best hydrogels with the highest swelling degree, gel fraction, cephalexin absorption, and antibacterial activity. The morphology and either absorption or release kinetics of cephalexin by the optimum prepared hydrogels were also investigated. No significant differences between the experimental and predicted data confirmed the suitability of the suggested models.

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Padina gymnospora derived a linear polysaccharide “alginate” mediated synthesis of silver nanocomposite and its antibacterial activity

Letters in Applied NanoBioScience ◽

10.33263/lianbs92.11361140 ◽

2020 ◽

Vol 9 (2) ◽

pp. 1136-1140

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Sodium Alginate ◽

Ag Nanoparticles ◽

Silver Ions ◽

Multi Drug Resistance ◽

Human Pathogens ◽

Electron Microscopic ◽

Sem Image ◽

Padina Gymnospora

Sodium alginate extract of Padina gymnospora was used for the synthesis of silver (Ag) nanoparticles. UV-visible spectroscopy studies were carried out to assess the formation of Ag nanoparticles. Reductions of Ag+ ions on sodium alginate were conformed to FTIR. Scanning Electron Microscopic (SEM-EDX) was used to characterize the Ag nanoparticles. SEM image divulges that silver nanoparticles were quite polydispersed, the size ranging from 54nm to 78nm. EDX spectroscopy also confirmed the presence of silver ions. AFM analysis did analyze the morphology of the nanocomposite. Biosynthesized silver nanoparticles showed the antibacterial activity against multi-drug resistance human pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. These results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to various medical devices and antimicrobial control systems.

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Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Scientific Reports ◽

10.1038/s41598-021-90208-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Helal F. Hetta ◽

Israa M. S. Al-Kadmy ◽

Saba Saadoon Khazaal ◽

Suhad Abbas ◽

Ahmed Suhail ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Acinetobacter Baumannii ◽

Biofilm Formation ◽

Microtiter Plate ◽

Diffusion Method ◽

Resistance Pattern ◽

Infection Model ◽

The Impact

AbstractWe aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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A comparative analysis of antibacterial activity, dynamics, and effects of silver ions and silver nanoparticles against four bacterial strains

International Biodeterioration & Biodegradation ◽

10.1016/j.ibiod.2017.07.015 ◽

2017 ◽

Vol 123 ◽

pp. 304-310 ◽

Cited By ~ 30

Author(s):

Wen-Ru Li ◽

Ting-Li Sun ◽

Shao-Lu Zhou ◽

Yong-Kai Ma ◽

Qing-Shan Shi ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Comparative Analysis ◽

Silver Ions ◽

Bacterial Strains ◽

Activity Dynamics

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Green Synthesis of Silver Nanoparticles using Salacca zalacca Extract as Reducing Agent and It’s Antibacterial Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.22238 ◽

2019 ◽

Vol 31 (12) ◽

pp. 2804-2810

Author(s):

Anti Kolonial Prodjosantoso ◽

Oktanio Sigit Prawoko ◽

Maximus Pranjoto Utomo ◽

Lis Permana Sari

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Silver Ions ◽

Reduction Reaction ◽

Face Centered Cubic ◽

Gram Positive Bacteria ◽

Transmission Electron ◽

Face Centered

In this article, the synthesis of silver nanoparticles through a reduction reaction process using Salacca zalacca extract is reported. The AgNPs were characterized using X-ray diffraction, transmission electron microscopy, Fourier transform infrared and UV-visible spectrophotometry methods. The AgNPs antibacterial activity was determined against of Gram-positive bacteria (Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli). The main functional groups contained in Salacca zalacca extract are carbonyl, hydroxyl and nitrile groups, which are believed to reduce the silver ions to metal. The surface plasmon resonance values of brownish red AgNPs are in the range of 410 nm to 460 nm. The structure of AgNPs is face centered cubic (FCC). The diameter of silver nanoparticles crystallite is 14.2 ± 2.6 nm. The AgNPs growth inhibition zones of Escherichia coli and Staphylococcus epidermidis are 9.6 mm and 9.2 mm, respectively.

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A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity

International Journal of Carbohydrate Chemistry ◽

10.1155/2013/539636 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Siraj Shaik ◽

Madhusudana Rao Kummara ◽

Sudhakar Poluru ◽

Chandrababu Allu ◽

Jaffer Mohiddin Gooty ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Leaf Extract ◽

Silver Ions ◽

Inhibition Zone ◽

Zone Method ◽

Green Approach ◽

Tridax Procumbens ◽

Poly Acrylamide

A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox polymerization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag+) into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications.

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Phytochemical Synthesis of Silver Nanoparticles Using Anthemis Nobilis Extract and Its Antibacterial Activity

Zeitschrift für Physikalische Chemie ◽

10.1515/zpch-2018-1288 ◽

2020 ◽

Vol 234 (3) ◽

pp. 531-540

Author(s):

Saba Ghamipoor ◽

Faeze Fayyazi ◽

Saeed Bahadorikhalili

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Nitrate Solution ◽

Synthesis Method ◽

Silver Ions ◽

Good Alternative ◽

Ft Ir ◽

Average Size ◽

Physical And Chemical

AbstractIn this work, green synthesis of silver nanoparticles is described by phytochemical reducing silver nitrate aqueous solution using Anthemis nobilis. For this purpose, Anthemis nobilis extract was used for the synthesis of silver nanoparticles as both surfactant and reducing agent. Green synthesis method is a good alternative to physical and chemical methods, since it is fast, simple, environmentally-friendly and economic. The produced nanoparticles are identified using FE-SEM, EDX, and FT-IR and Uv/Vis techniques. Formation of silver nanoparticles is verified in 430–420 nm range. Reduction of silver ions by hydroxyl functional group is also confirmed by FT-IR device. EDX device confirms the presence of a peak for Ag element without any impurity peak. Silver nanoparticles are identified by FE-SEM device and found to have average size between 17 and 42 nm. Also, the antibacterial activity of the synthesized nanoparticles is compared with that of staphyloccusaureus and pseudomonasa aeruginosa and the maximum inhibitory activity against the bacteria is obtained using 1 mM nitrate solution.

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