Plasma membrane N-glycoproteome analysis of wheat seedling leaves under drought stress

Metabolomics is an effective biotechnological tool that can be used to attain comprehensive information on metabolites. In this study, the profiles of metabolites produced by wheat seedlings in response to drought stress were investigated using an untargeted approach with ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to determine various physiological processes related to drought tolerance from the cross between drought-tolerant genotype (HX10) and drought-sensitive genotype (YN211). The current study results showed that under drought stress, HX10 exhibited higher growth indices than YN211. After drought stress treatment, a series of phenolics accumulated higher in HX10 than in YN211, whereas the amount of thymine, a pyrimidine, is almost 13 folds of that in YN211. These metabolites, as well as high levels of different amino acids, alkaloids, organic acids, and flavonoids in the drought treated HX10 could help to explain its strong drought-tolerant capacity. The current study explored the understanding of the mechanisms involved in the drought response of wheat seedling; these metabolome data could also be used for potential QTL or GWAS studies to identify locus (loci) or gene(s) associated with these metabolic traits for the crop improvement.

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A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gms096 ◽

2012 ◽

Vol 45 (2) ◽

pp. 104-114 ◽

Cited By ~ 18

Author(s):

J. Zhang ◽

D. Li ◽

D. Zou ◽

F. Luo ◽

X. Wang ◽

...

Keyword(s):

Plasma Membrane ◽

Drought Stress ◽

Seedling Development ◽

Gene Encoding

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The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley

International Journal of Molecular Sciences ◽

10.3390/ijms20174111 ◽

2019 ◽

Vol 20 (17) ◽

pp. 4111 ◽

Cited By ~ 4

Author(s):

Kangfeng Cai ◽

Huaizhou Gao ◽

Xiaojian Wu ◽

Shuo Zhang ◽

Zhigang Han ◽

...

Keyword(s):

Plasma Membrane ◽

Drought Stress ◽

Drought Tolerance ◽

Transcript Level ◽

Genotypic Difference ◽

K Uptake ◽

Significant Difference ◽

Specific Manner ◽

Barley Genotypes

In this work, the effect of drought on K+ uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+ uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+ contents and drought tolerance levels between the contrasting barley genotypes. The role of K+ transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+ uptake (HvHAK1, HvHAK5, HvKUP1, HvKUP2 and HvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+ transporters and channels is critical for root K+ uptake and root to shoot K+ translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+ efflux and K+ influx under drought stress, which was further confirmed by the significant up-regulation of HvHA1 and HvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+ transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+ transporters and channels in root epidermis and stele.

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