scholarly journals Coexistence of opportunistic mycosis and mycobacterium tuberculosis in patients attending the Central Tuberculosis Reference Laboratory of Ghaemshahr city, Iran

2015 ◽  
Vol 4 ◽  
pp. 129 ◽  
Author(s):  
Mohammad Reza Jabbari ◽  
Tahereh Shokohi ◽  
Mahdi Abastabar ◽  
Seyed Reza Aghili ◽  
Masoud Aliyali ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Reference Laboratory ◽  
Opportunistic Mycosis

Proficiency testing of conventional drug susceptibility tests of Mycobacterium tuberculosis

1987 ◽  
Vol 33 (12) ◽  
pp. 1064-1068 ◽  
Author(s):  
Adalbert Laszlo ◽  
Dorothy M. Helbecque ◽  
Walter Tostowaryk
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Proficiency Testing ◽  
Susceptibility Testing ◽  
Developed Countries ◽  
Drug Susceptibility ◽  
Reference Laboratory ◽  
In Vitro Susceptibility ◽  
Testing Procedures ◽  
Drug Concentrations ◽  
Susceptibility Tests

Proficiency testing of indirect drug susceptibility tests of Mycobacterium tuberculosis was begun in 1985 by the Laboratory Centre for Disease Control (LCDC) with the participation of Provincial Public Health Laboratories in Canada. Comparable sets of 60 cultures of Mycobacterium tuberculosis representing 30 strains were distributed by LCDC to the participating laboratories to be tested for drug susceptibility against isoniazid, streptomycin, rifampin, and ethambutol using conventional methodologies. Intralaboratory agreement values determined by comparing results obtained on sets of duplicate cultures were high and were found to vary little from drug to drug and from laboratory to laboratory. Interlaboratory agreement was determined by comparing results reported by participating laboratories to those obtained by the Reference Laboratory. Agreement percentages were found to be lower for drug-resistant cultures than for drug-susceptible cultures. The reliability of drug susceptibility testing results was higher for isoniazid and rifampin, than for ethambutol and streptomycin. This study shows that the higher subsidiary drug concentrations do not compare well with main drug concentrations, especially in the case of streptomycin and ethambutol. The significance of the higher subsidiary concentrations in in vitro susceptibility testing is therefore in need of clarification. The proficiency testing results obtained in this study compare favorably with those reported in other developed countries despite the fact that a variety of testing procedures are used throughout the country.

scholarly journals Diagnostic value of serum IgG by Eliza to detecting Mycobacterium tuberculosis, Original Article

2021 ◽  
Vol 9 (1) ◽  
pp. 29-29
Author(s):  
Mehdi Haghdoost ◽  
Parisa Alizadeh Nazmi ◽  
Hamid Owaysee Osquee
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Immunoglobulin G ◽  
Diagnostic Value ◽  
World Health ◽  
Control Group ◽  
Reference Laboratory ◽  
Predictive Values ◽  
Serum Igg ◽  
Significant Difference

Introduction: In developing countries, which is an endemic region in terms of tuberculosis, there is an urgent need for fast, accurate, and inexpensive serological testing. The aim of this study was to determine the diagnostic value of patient serum IgG antibodies by ELISA in the diagnosis of Mycobacterium tuberculosis. Method: This case-control study was performed on patients with pulmonary tuberculosis in 2017-2020. After selecting the case (n = 30) and control (n = 30) subjects according to inclusion criteria, their blood samples were obtained and analyzed in the reference laboratory by standard kits for immunoglobulin G against 16, 36, and 40 kDa antigens of mycobacterium tuberculosis. Results: The mean age of the subjects was 47.07 (15.57%). The majority of participants were 46 (51.1%) women. There was no significant difference between the two groups regarding sex and age. serological examination of patients with pulmonary tuberculosis showed 25 positive results and only 4 of the control group had a positive result. Sensitivity, specificity, positive and negative predictive values of serology test were 83.3%, 86.67%, 86.20% and 87.88% respectively. Conclusion: Despite the acceptable sensitivity of the serologic immunoglobulin G test, according to the statement of World health organization (WHO), it did not possess an acceptable specificity. It is recommended that a a wider range of different antigens to be studied also it is essential to evaluate the diagnostic value of the other immunoglobulins inpatient in different stages of the disease.

scholarly journals Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar

2020 ◽  
Vol 14 (11) ◽  
pp. 1296-1305
Author(s):  
Waing Waing Moe Sann ◽  
Wises Namwat ◽  
Kiatichai Faksri ◽  
Thyn Lei Swe ◽  
Kyi Kyi Swe ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Tuberculosis ◽  
Transmission Rate ◽  
Reference Laboratory ◽  
Epidemiology Study ◽  
Molecular Tools ◽  
Combined Method ◽  
Recent Transmission ◽  
Lower Transmission ◽  
Expected Proportion

Introduction: MIRU-VNTR typing and Spoligotyping are the useful molecular tools for TB epidemiology study. Information regarding genetic diversity and tuberculosis (TB) transmission in Upper Myanmar only is scares. Methodology: We determined the genetic diversity of Mycobacterium tuberculosis (Mtb) and TB transmission from Upper Myanmar TB Reference Laboratory, Mandalay Region, including Mandalay (72), Shan (22), Magway (15), Sagaing (13), Nay Pyi Taw (8), Kachin (7), Chin (2) and Kayah (1). One hundred and forty Mtb isolates were genotyped using 24-locus MIRU-VNTR typing and spoligotyping. Lineage classification and TB transmission analysis were performed. Results: 24-locus MIRU-VNTR typing identified 135 unique profiles and two clusters compared to 35 spoligotyping profiles which contained 12 clusters and 23 unique isolates, Beijing (n=100, 71.4%) was found to be prominent lineage by combine two methods. The expected proportion attributable to recent transmission based on clustering rate was 2.1%. One cluster case was more likely to be in MDR patient. Conclusions: Our findings showed Beijing genotypes were dominant in Upper Myanmar. The usage and analysis of 24-locus MIRU-VNTR typing might prove useful for our broader understanding of TB outbreaks and epidemiology than spoligotyping. The genotypic pattern of this combined method suggests that the lower transmission rate may be due to a higher possibility of reactivation cases in Upper Myanmar.

scholarly journals In-House Phage Amplification Assay Is a Sound Alternative for Detecting Rifampin-Resistant Mycobacterium tuberculosis in Low-Resource Settings

2005 ◽  
Vol 49 (1) ◽  
pp. 425-427 ◽  
Author(s):  
Norberto Símboli ◽  
Howard Takiff ◽  
Ruth McNerney ◽  
Beatriz López ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Predictive Value ◽  
Hot Spot ◽  
Multidrug Resistant ◽  
Work Flow ◽  
Reference Laboratory ◽  
Low Resource ◽  
Spot Area ◽  
Routine Work ◽  
Amplification Assay

ABSTRACT An in-house mycobacteriophage amplification assay for detecting rifampin-resistant Mycobacterium tuberculosis showed 100% sensitivity, 97.7% specificity, and 95.2% predictive value for resistance in a test of 129 isolates from a hot spot area of multidrug-resistant M. tuberculosis. The applicability of the test was demonstrated in the routine work flow of a low-resource reference laboratory.

scholarly journals Multi-Drug Resistance of Mycobacterium Tuberculosis – the Problem of Modern Phthisiology

2019 ◽  
Vol 4 (2) ◽  
pp. 55-59
Author(s):  
E. Yu. Zorkaltseva ◽  
O. A. Vorobyeva ◽  
E. D. Savilov ◽  
O. B. Ogarkov
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Russian Federation ◽  
Rural Areas ◽  
Diagnostic Methods ◽  
Multi Drug Resistance ◽  
High Morbidity ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Irkutsk Region ◽  
The Russian Federation

The 21st century is characterized by the exacerbation of the problem formation and spreading of drug-resistant strains throughout the world. Genetic mutations of Mycobacterium tuberculosis lead to the formation of drug-resistant forms because of long-term use of anti-tuberculosis drugs. The Russian Federation is among the top three countries with a high burden of tuberculosis with multidrug resistance (MDR). The estimated number of cases of tuberculosis with MDR in the Russian Federation was 60,000, which corresponds to half the burden of the European Region in 2015. In the Irkutsk region from 2014 till 2018 the proportion of TB cases with MDR cases increased from 15.2 % to 18.3 %. According to the reference laboratory of the Irkutsk Regional Clinical Tuberculosis Hospital, a significantly higher level of MDR among primary diagnosed was registered in the cities (27.7 %) and the lowest in rural areas of the region (16.5 %). Among the cohorts of patients with tuberculosis, the highest proportion of MDR was in the northern territories of the region (43.1 %), in Irkutsk district (41.2 %) and in large cities, including Irkutsk (38.5 %). Positive correlations were established between cohort of primary diagnosed TB with MDR and among populations in areas with high morbidity along the railway (r = 0.91; p = 0.00001), in the Irkutsk region (r = 0.89; p = 0,00008), and also in the Irkutsk city (r = 0.91; p = 0.00002). This is probably due to the influence of reservoir of tuberculosis infection formed in these localities. The regulatory documents of the Ministry of Health of the Irkutsk Region was developed on the basis of the data obtained, and they include recommendations for improving the diagnosis of tuberculosis in the region using fast and accelerated microbiological diagnostic methods.

Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by INNO-LiPA Rif.TB assay: retrospective analyses of Health Protection Agency National Mycobacterium Reference Laboratory data

2010 ◽  
Vol 59 (5) ◽  
pp. 563-566 ◽  
Author(s):  
Srinivasulu Reddy ◽  
Timothy Brown ◽  
Francis Drobniewski
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Laboratory Data ◽  
Clinical Specimen ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Molecular Testing ◽  
Reference Laboratory ◽  
Clinical Probability ◽  
Tissue Specimens ◽  
The Uk

Molecular diagnostic methods are of potential value in identifying tuberculosis (TB) and drug resistance where tissue specimens have been submitted for histology but not for microbiological culture. All paraffin-embedded tissue (PET) specimens (n=60) referred to a single national centre over a 42 month study period were analysed using the INNO-LiPA Rif.TB assay; 29/60 patients had been reported to the UK Enhanced Tuberculosis Surveillance database with a diagnosis of TB based on clinical, radiological and histological evidence. Mycobacterium tuberculosis (MTB) DNA was detectable in 5 out of 29 reported TB cases (17.2 %); 12 out of 29 of the reported TB cases had a positive MTB culture from a secondary clinical specimen and MTB DNA was detectable in 2 of the 12 (16.7 %) laboratory-confirmed TB cases. Referring clinicians should be aware of the limitations of this assay on PETs, and should request molecular testing only in patients with a high clinical probability of TB and when acid-fast bacilli are seen in tissue specimens.

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