scholarly journals Evaluation of Serum Levels of Osteopontin and IgG Anti-Osteopontin Autoantibodies As Potential Biomarkers of Immune Activation in Patients with Allergic Diseases

2016 ◽  
Vol 137 (2) ◽  
pp. AB394
Author(s):  
Elisa Villa ◽  
Rosalba Minisini ◽  
Olaf Rötzschke ◽  
Kia Joo Puan ◽  
Boris Buenbrazo San Luis ◽  
...  
Keyword(s):  
Immune Activation ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Potential Biomarkers

scholarly journals Circulating miRNA-181b-5p, miRNA-223-3p, miRNA-210-3p, let 7i-5p, miRNA-21-5p and miRNA-29a-3p in patients with localized scleroderma as potential biomarkers

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Katarzyna Wolska-Gawron ◽  
Joanna Bartosińska ◽  
Marta Rusek ◽  
Małgorzata Kowal ◽  
Dorota Raczkiewicz ◽  
...  
Keyword(s):  
Longitudinal Research ◽  
Clinical Symptoms ◽  
Serum Levels ◽  
Surfactant Protein ◽  
Localized Scleroderma ◽  
Surfactant Protein D ◽  
Chemokine Ligand ◽  
Dipeptidylpeptidase 4 ◽  
Potential Biomarkers

AbstractLocalized scleroderma (LoSc) is a rare disease manifested by an inflammation and sclerosis of the skin. The latest studies focused on glycoprotein Krebs von den Lungen-6, surfactant protein-D, chemokine ligand 18 and dipeptidylpeptidase 4 as potential biomarkers of skin fibrosis in systemic scleroderma. Our study aimed to identify 6 miRNAs with elevated or decreased levels in 38 LoSc patients (31 females, 7 males) compared to healthy volunteers (HVs) and to correlate the selected miRNAs’ serum levels with the severity and the clinical symptoms of LoSc and some laboratory parameters with the selected miRNAs’ serum levels. The serum levels of miRNAs, i.e. miRNA-181b-5p, miRNA-223-3p, miRNA-21-5p, let 7i-5p, miRNA-29a-3p and miRNA-210-3p were significantly increased in the LoSc patients compared to the HVs. The level of let-7i increase in the female LoSc patients correlated negatively with BSA (r =  − 0.355, p = 0.049) and mLoSSI (r =  − 0.432, p = 0.015). Moreover, the female patients with inactive LoSc had significantly higher level of let-7i (2.68-fold on average) in comparison to those with active disease (p = 0.045). The exact role of those molecules has not been revealed in LoSc and a long-term longitudinal research is pivotal to confirm their prognostic value.

scholarly journals The Study of a Possible Correlation between Serum Levels of Interleukin 17 and Clinical Severity in Patients with Allergic Rhinitis

2017 ◽  
Vol 8 (3) ◽  
pp. ar.2017.8.0207
Author(s):  
Mai Aly Gharib Aly ◽  
Mohamed Tawfik El Tabbakh ◽  
Waheed Fawzy Heissam ◽  
Said Hamed Abbadi
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Clinical Severity ◽  
Total Serum ◽  
Interleukin 17 ◽  
Serum Ige ◽  
Cluster Immunotherapy

Introduction Allergic rhinitis (AR) is one of the most common allergic diseases, which affects ~20% of the world's population. T-helper (Th) type 2 cells produce interleukin (IL) 4 and IL-13, and mediate allergic responses, and these cytokines have been extensively studied as key players in the atopic airway diseases. However, the involvement of Th17 cells and IL-17 in AR has not been clearly examined. Aim To reevaluate AR clinical severity with serum IL-17, whether IL-17 affects the disease alone or in contribution with the atopic predisposition. Patients and Methods During an 18-month period, 39 individuals were divided into three groups: A, (13 control), B (13 with mild-to-moderate AR), and C (13 with severe AR). Both group B and group C patients (26) were subjected to clinical examination and allergy skin testing, and to measurement of both total serum immunoglobulin E (IgE) and IL-17 levels. Eleven patients with AR then were exposed to 6 months of cluster immunotherapy, whereas the rest of the patients were not exposed. Results Revealed a significant elevation of serum IL-17 levels with an associated increase in serum IgE in the patients with AR compared with controls and revealed that the serum levels of both total serum IgE and IL-17 decreased significantly after cluster immunotherapy. Conclusion These preliminary results added new data about the use of injective immunotherapy as well as reported on the use of sublingual immunotherapy.

Biomarkers in vesicoureteral reflux: an overview

2020 ◽  
Vol 14 (8) ◽  
pp. 683-696
Author(s):  
Flávia C Valério ◽  
Renata D Lemos ◽  
Ana L de C Reis ◽  
Letícia P Pimenta ◽  
Érica LM Vieira ◽  
...  
Keyword(s):  
Vesicoureteral Reflux ◽  
Renal Scarring ◽  
Reflux Nephropathy ◽  
Serum Levels ◽  
Cellular Adhesion ◽  
High Grade ◽  
Epigenetic Factors ◽  
Cellular Adhesion Molecules ◽  
Potential Biomarkers

Aim: This article aimed to review the role of cytokines, chemokines, growth factors and cellular adhesion molecules as biomarkers for vesicoureteral reflux (VUR) and reflux nephropathy (RN). Methods: We reviewed articles from 1979 onward by searching PubMed and Scopus utilizing the combination of words: ‘VUR’ or ‘RN’ and each one of the biomarkers. Results: Genetic, inflammatory, fibrogenic, environmental and epigenetic factors responsible for renal scarring need to be better understood. TGF-β, IL-10, IL-6, IL-8 and TNF seem to exert a role in VUR particularly in RN based on the current literature. Serum levels of procalcitonin have been also associated with high-grade VUR and RN. These molecules should be more intensively evaluated as potential biomarkers for renal scarring in VUR. Conclusion: Further studies are necessary to define which molecules will really be of utility in clinical decisions and as therapeutic targets for VUR and RN.

scholarly journals Clinical Significance of Serum Galectin-9 and Soluble CD155 Levels in Patients with Systemic Sclerosis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Mami Chihara ◽  
Miki Kurita ◽  
Yuki Yoshihara ◽  
Akihiko Asahina ◽  
Koichi Yanaba
Keyword(s):  
Immune Response ◽  
Systemic Sclerosis ◽  
T Cell ◽  
Clinical Features ◽  
Sedimentation Rate ◽  
Immune Activation ◽  
Serum Levels ◽  
Organ Involvement ◽  
Erythrocyte Sedimentation ◽  
Domain 3

Signaling through coinhibitory receptors downregulates the immune response to prevent excessive immune activation and maintain optimal immunity and tolerance. The aim of this study was to examine the levels of the soluble forms of coinhibitory receptors and their ligands, namely, galectin-9 (the ligand of T-cell immunoglobulin and mucin domain 3) and CD155 (the ligand of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain), and their association with clinical features in patients with systemic sclerosis (SSc). The serum levels of galectin-9 and soluble sCD155 were examined by enzyme-linked immunosorbent assays in patients with SSc, and the results were evaluated with respect to clinical features. Patients with SSc exhibited raised serum levels of galectin-9, but not sCD155. Serum galectin-9 levels were raised not only in patients with diffuse cutaneous SSc but also in patients with limited cutaneous SSc. Furthermore, serum galectin-9 levels correlated positively with the erythrocyte sedimentation rate. In addition, increased serum galectin-9 levels tended to be associated with higher mortality and serious organ involvement. These results suggest that galectin-9, but not CD155, may be involved in the pathogenesis of SSc. In addition, the measurement of serum galectin-9 levels could be used to predict serious organ involvement and high mortality in patients with SSc.

Assessment of serum levels of allergen-specific immunoglobulin E in different seasons and breeds in healthy horses

2014 ◽  
Vol 17 (2) ◽  
pp. 331-337 ◽  
Author(s):  
P.M. Wilkołek ◽  
Z.J.H. Pomorski ◽  
M.P. Szczepanik ◽  
Ł. Adamek ◽  
M. Pluta ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Winter Season ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Specific Ige ◽  
Diagnostic Tools ◽  
Storage Mites ◽  
Acarus Siro ◽  
Different Seasons ◽  
Domestic Mites

Abstract The present study was designed to asses specific IgE towards environment allergens in 42 healthy horses. Determination of this immunoglobulin in serum serve as diagnostic tools in allergic diseases to improve efficacy of the treatment and proper allergen selection to specific immunotherapy. Serum levels of allergen specific IgE were measured with equine monoclonal antibody, using 15 individual and 5 mix allergens in North European Panel. The study revealed season dependent increased levels of allergen specific IgE in normal horses. It is noteworthy that healthy horses show high percentage of positive reactions, most commonly towards to domestic mites D. farinae (80%), D. pteronyssinus (35.71%) and storage mites T. putrenscentiae (42.86%), Acarus siro (40.48%). These allergens play an important role in equine, canine and feline atopic dermatitis. We also demonstrated high IgE levels in the group of horse specific insect allergens. Tabanus sp. (35.71%), Culicoides sp. (28.57%) and Simulium sp. (26.19%) were the most frequent insect positive reaction allergens. No positive reactions in all groups of allergens were found in winter season, low and merely detectable levels of antibodies have been found relating to D. farianae and T. putrescentiae allergen. We observed elevated mould-IgE levels in horses that live in stables, while outdoor living horses showed very low levels. Amongst all positive reactions we observed only weak and moderate reactions but no strong positive reactions were found. No significant differences were observed between three breeds of horses with the exception of moulds and D. pteronyssinus allergens

scholarly journals Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin

2005 ◽  
Vol 202 (4) ◽  
pp. 541-549 ◽  
Author(s):  
Jane Yoo ◽  
Miyuki Omori ◽  
Dora Gyarmati ◽  
Baohua Zhou ◽  
Theingi Aye ◽  
...  
Keyword(s):  
Animal Model ◽  
Atopic Dermatitis ◽  
Transgenic Mice ◽  
Elevated Serum ◽  
Allergic Inflammation ◽  
Disease Process ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Thymic Stromal Lymphopoietin ◽  
Common Disease

The cytokine thymic stromal lymphopoietin (TSLP) has recently been implicated in the pathogenesis of atopic dermatitis (AD) and other allergic diseases in humans. To further characterize its role in this disease process, transgenic mice were generated that express a keratinocyte-specific, tetracycline-inducible TSLP transgene. Skin-specific overexpression of TSLP resulted in an AD-like phenotype, with the development of eczematous lesions containing inflammatory dermal cellular infiltrates, a dramatic increase in Th2 CD4+ T cells expressing cutaneous homing receptors, and elevated serum levels of IgE. These transgenic mice demonstrate that TSLP can initiate a cascade of allergic inflammation in the skin and provide a valuable animal model for future study of this common disease.

scholarly journals Galectin-3, osteopontin and successful aging

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Fabian Sanchis-Gomar ◽  
Alejandro Santos-Lozano ◽  
Helios Pareja-Galeano ◽  
Nuria Garatachea ◽  
Rafael Alis ◽  
...  
Keyword(s):  
Successful Aging ◽  
Area Under The Curve ◽  
Predictor Variable ◽  
Serum Levels ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Galectin 3 ◽  
Medical Interest ◽  
Potential Use ◽  
Potential Biomarkers

AbstractBackground:Individuals who reach exceptional longevity (100+ years of age) free of common chronic age diseases (i.e. ‘dodgers’) arguably represent the paradigm of successful aging in humans. As such, identification of potential biomarkers associated with this phenomenon is of medical interest.Methods:We measured serum levels of galectin-3 and osteopontin, both of which have been shown to be linked with major chronic or aging-related disorders in younger populations, in centenarian ‘dodgers’ (n=81; 40 men; 100–104 years) and healthy controls (n=41; 24 men, 70–80 years).Results:Both biomarkers showed significantly lower values (p<0.001) in the former (galectin-3: 2.4±1.7 vs. 4.8±2.8 ng/mL; osteopontin: 38.1±27.7 vs. 72.6±33.1 μg/mL). Logistic regression analysis identified the combination of these two biomarkers as a significant predictor variable associated with successful aging regardless of sex (p<0.001). The area under the curve (AUC) classified the ability of galectin-3 and osteopontin to predict the likelihood of successful aging as ‘fair’ (AUC=0.75) and ‘good’ (AUC=0.80), respectively. Particularly, the combination of the two biomarkers showed good discriminatory power for successful aging (AUC=0.86), with sensitivity=83% and specificity=74%.Conclusions:Lower levels of both galectin-3 and osteopontin are associated with successful aging, representing potential biomarkers of this condition. Our cross-sectional data must be however approached with caution. Further research is necessary to replicate the present preliminary results in other cohorts and to identify the potential use of galectin-3 and osteopontin as potential targets (or at least predictors) in future personalized anti-aging therapies.

Serum Levels of Galectin-3: Possible Association with Fibrosis, Aberrant Angiogenesis, and Immune Activation in Patients with Systemic Sclerosis

2012 ◽  
Vol 39 (3) ◽  
pp. 539-544 ◽  
Author(s):  
TAKASHI TANIGUCHI ◽  
YOSHIHIDE ASANO ◽  
KANAME AKAMATA ◽  
SHINJI NODA ◽  
YURI MASUI ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Immune Activation ◽  
Developmental Process ◽  
Systolic Pressure ◽  
Serum Levels ◽  
Digital Ulcers ◽  
Healthy Controls ◽  
Ventricular Systolic Pressure ◽  
Multifunctional Protein ◽  
Galectin 3

Objective.Galectin-3 is a multifunctional protein implicated in a variety of biological processes including fibrosis, angiogenesis, and immune activation, all of which are associated with the development of systemic sclerosis (SSc). We investigated the clinical significance of serum galectin-3 levels in SSc.Methods.Serum galectin-3 levels were determined by a specific ELISA in 58 patients with SSc and 19 healthy controls.Results.Serum galectin-3 levels were significantly lower in patients with diffuse cutaneous SSc (dcSSc) than in controls (3.29 ± 3.27 ng/ml vs 4.91 ± 2.67 ng/ml, respectively; p < 0.05), while being comparable between limited cutaneous SSc (3.70 ± 2.39 ng/ml) and healthy controls. In dcSSc, serum galectin-3 levels significantly correlated with total skin score (r = 0.45, p < 0.05). Serum galectin-3 levels were significantly decreased in early dcSSc (disease duration < 1 year; 1.64 ± 1.74 ng/ml; p < 0.05), but not in mid-stage dcSSc (1 to 6 years; 3.22 ± 3.16 ng/ml) or late-stage dcSSc (> 6 years; 4.86 ± 4.10 ng/ml), compared with controls. Serum galectin-3 levels were higher in SSc patients with both digital ulcers (DU) and elevated right ventricular systolic pressure (RVSP) than in those without each symptom (DU: 5.44 ± 3.74 ng/ml vs 2.99 ± 2.36 ng/ml, p < 0.05; elevated RVSP: 4.44 ± 3.14 ng/ml vs 2.82 ± 2.64 ng/ml, p < 0.05).Conclusion.Galectin-3 may be related to the developmental process of skin sclerosis in dcSSc and of DU and pulmonary vascular involvements in total SSc.

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