scholarly journals Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies

2010 ◽  
Vol 150 (2) ◽  
pp. 232-239 ◽  
Author(s):  
Fernie Goh ◽  
Jeffrey D. Gross ◽  
Nicholas E. Simpson ◽  
Athanassios Sambanis
Keyword(s):  
Encapsulated Cells ◽  
Beneficial Effects ◽  
Cells In Culture ◽  
Modeling Studies

scholarly journals The amount range of membrane cholesterol required for robust cell adhesion and proliferation in serum-free condition.

2021 ◽  
Author(s):  
Shino Takii ◽  
Jun Wu ◽  
Daiji Okamura
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Attachment ◽  
Cancer Cell Lines ◽  
Membrane Cholesterol ◽  
Cholesterol Depletion ◽  
Serum Free ◽  
Beneficial Effects ◽  
Cells In Culture

Serum-containing medium is widely used to support cell attachment, stable growth and serial passaging of various cancer cell lines.   However, the presence of cholesterols and lipids in serum greatly hinders the analysis of the effects of cholesterol depletion on cells in culture.   In this study, we develop a defined serum-free culture condition accessible to a variety of different types of adherent cancer cells. We tested different factors that are considered essential for cell culture and various extracellular matrix for plate coating, and found cells cultured in Dulbecco's Modified Eagle's Medium (DMEM) basal media supplemented with Albumin (BSA) and insulin-transferrin-selenium-ethanolamine (ITS-X) on fibronectin-precoated well (called as “DA-X condition”) showed comparable proliferation and survival to those in a serum-containing medium. Interestingly, we observed that DA-X condition could be adapted to a wide variety of adherent cancer cell lines, which enabled the analysis of how cholesterol depletion affected cancer cells in culture. Mechanistically, we found the beneficial effects of the DA-X condition in part can be attributed to the appropriate level of membrane cholesterol, and fibronectin-mediated signaling plays an important role in the suppression of cholesterol production.

scholarly journals Study of Bifidobacterium Lactic 300b Survival during Encapsulation, Coating and Freeze Drying Process and the Release in Alkaline Media

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Oana Lelia POP ◽  
Thorsten BRANDAU ◽  
Dan Cristian VODNAR ◽  
Carmen SOCACIU
Keyword(s):  
Freeze Drying ◽  
Dip Coating ◽  
Alkaline Media ◽  
Drying Process ◽  
Bifidobacterium Lactis ◽  
Coating Application ◽  
Encapsulated Cells ◽  
Beneficial Effects ◽  
Freeze Dried ◽  
Coating Method

The survival of probiotics is of paramount importance because to have their beneficial effects on the host’s health, they must stay alive up to the site of their action. It is known that the resistance of probiotic cells in acidic media, as in the stomach, is very low, that’s why the encapsulation of Bifidobacterium lactis 300B in alginate matrices is needed. This study present the mechanism of cell survival during encapsulation, coating and freeze drying process and release in simulated intestinal media. Microspheres with mean diameter 1100 µm and 66.87% encapsulation rate were obtained and described. In order to increase the protection of Bifidobacterium lactis 300B in the alginate/pullulan based microspheres, three types of dip coating were proposed. Namely, the alginate microspheres were coated in alginate, chitosan or gelatin, using the dip coating method by crosslinking. The survival of the cells was tested after encapsulation, coating application and compared with free and encapsulated cells, using the plate counting method. Also, the survivability in freeze dried microspheres was tested. Although the coated microspheres showed higher survivability compared with free and encapsulated cells, the release in alkaline pH was no possible for the gelatin coated alginate/pullulan based microspheres. 

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

Nuclear receptors in disease: the oestrogen receptors

2004 ◽  
Vol 40 ◽  
pp. 157-167 ◽  
Author(s):  
Maria Nilsson ◽  
Karin Dahlman-Wright ◽  
Jan-Åke Gustafsson
Keyword(s):  
Nuclear Receptors ◽  
Target Genes ◽  
Receptor Subtype ◽  
Target Tissue ◽  
Oestrogen Receptors ◽  
Nucleotide Polymorphisms ◽  
Cell Type ◽  
Single Nucleotide ◽  
Beneficial Effects ◽  
The Family

For several decades, it has been known that oestrogens are essential for human health. The discovery that there are two oestrogen receptors (ERs), ERalpha and ERbeta, has facilitated our understanding of how the hormone exerts its physiological effects. The ERs belong to the family of ligand-activated nuclear receptors, which act by modulating the expression of target genes. Studies of ER-knockout (ERKO) mice have been instrumental in defining the relevance of a given receptor subtype in a certain tissue. Phenotypes displayed by ERKO mice suggest diseases in which dysfunctional ERs might be involved in aetiology and pathology. Association between single-nucleotide polymorphisms (SNPs) in ER genes and disease have been demonstrated in several cases. Selective ER modulators (SERMs), which are selective with regard to their effects in a certain cell type, already exist. Since oestrogen has effects in many tissues, the goal with a SERM is to provide beneficial effects in one target tissue while avoiding side effects in others. Refined SERMs will, in the future, provide improved therapeutic strategies for existing and novel indications.

ACE2 as therapeutic agent

2020 ◽  
Vol 134 (19) ◽  
pp. 2581-2595
Author(s):  
Qiuhong Li ◽  
Maria B. Grant ◽  
Elaine M. Richards ◽  
Mohan K. Raizada
Keyword(s):  
Therapeutic Agent ◽  
Renin Angiotensin System ◽  
Peptide Hormone ◽  
Experimental Models ◽  
Electrolyte Balance ◽  
Ang Ii ◽  
Angiotensin Converting Enzyme 2 ◽  
Beneficial Effects ◽  
Overwhelming Evidence ◽  
Future Challenges

Abstract The angiotensin-converting enzyme 2 (ACE2) has emerged as a critical regulator of the renin–angiotensin system (RAS), which plays important roles in cardiovascular homeostasis by regulating vascular tone, fluid and electrolyte balance. ACE2 functions as a carboxymonopeptidase hydrolyzing the cleavage of a single C-terminal residue from Angiotensin-II (Ang-II), the key peptide hormone of RAS, to form Angiotensin-(1-7) (Ang-(1-7)), which binds to the G-protein–coupled Mas receptor and activates signaling pathways that counteract the pathways activated by Ang-II. ACE2 is expressed in a variety of tissues and overwhelming evidence substantiates the beneficial effects of enhancing ACE2/Ang-(1-7)/Mas axis under many pathological conditions in these tissues in experimental models. This review will provide a succinct overview on current strategies to enhance ACE2 as therapeutic agent, and discuss limitations and future challenges. ACE2 also has other functions, such as acting as a co-factor for amino acid transport and being exploited by the severe acute respiratory syndrome coronaviruses (SARS-CoVs) as cellular entry receptor, the implications of these functions in development of ACE2-based therapeutics will also be discussed.

174 Early beneficial effects of vasopeptidase inhibtion n an experimental model of heart failure

2003 ◽  
Vol 2 (1) ◽  
pp. 33-34
Author(s):  
B SHIVALKAR ◽  
B MEURIS ◽  
R VANBENEDEN ◽  
J KETESLEGERS ◽  
F BECKERS ◽  
...  
Keyword(s):  
Heart Failure ◽  
Experimental Model ◽  
Beneficial Effects
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