AflatoxinB1 was extracted from rice contaminated artificially with Aspergillus flavus by
using methanol-water (50:50 v/v). As for experiment group, sodium chloride was added into the
extracted solution of AfB1, the excitation wavelength of 365 nm under the fluorescence mode, and
the emission spectrum peak at 440nm were observed. To study the detoxification of AflatoxinB1 in
several alkaline solutions, the solution of alkaline with difference concentration was added into the
solution of AfB1 respectively. The results showed that all of three alkaline solutions could decrease
the 440nm emission spectrum peak of solution of AfB1 in varying degrees, the effect to decrease
emission spectrum peak of AfB1 was very obvious in sodium hypochlorite (NaClO), and sodium
hydroxide (NaOH) took second place, whereas in ammonia (NH3·H2O), the effect was the weakest
among three alkaline solutions. Moreover, the effects were different in the same alkaline solutions
with different concentration, as hydrochloric acid (HCl) in low concentration was added to this
complex AfB1 alkaline solutions, detoxification efficacy of AfB1 were found to be obviously
increased. It was concluded that the detoxification of AflatoxinB1 may be affected by alkaline
solutions according to their concentration and be degraded greatly by acid addition.