Cell membrane-formed nanovesicles for disease-targeted delivery

Liposomes are bilayer membrane vesicles that can serve as vehicles for drug delivery. They are a good alternative to free drug administration that provides cell-targeted delivery into tumors, limiting the systemic toxicity of chemotherapeutic agents. Previous results from our group showed that an astrocytoma cell line exhibits selective uptake of sulfatide-rich (SCB) liposomes, mediated by the low-density lipoprotein receptor (LDL-R). The goal of this study was to assess the uptake of liposomes in a neuroblastoma cell line. For this purpose, we used two types of liposomes, one representing a regular cell membrane (DOPC) and another rich in myelin components (SCB). An astrocytoma cell line was used as a control. Characterization of liposome uptake and distribution was conducted by flow cytometry and fluorescence microscopy. Similar levels of LDL-R expression were found in both cell lines. The uptake of SCB liposomes was higher than that of DOPC liposomes. No alterations in cell viability were found. SCB liposomes were located near the cell membrane and did not colocalize within the acidic cellular compartments. Two endocytic pathway inhibitors did not affect the liposome uptake. Neuroblastoma cells exhibited a similar uptake of SCB liposomes as astrocytoma cells; however, the pathway involved appeared to be different than the hypothesized pathway of LDL-R clathrin-mediated endocytosis.

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Co-biomembrane coated Fe3O4/MnO2 multifunctional nanoparticles for targeted delivery and enhanced hemodynamic/photothermal/chemo therapy

Chemical Communications ◽

10.1039/d1cc01375k ◽

2021 ◽

Author(s):

Yingshu Guo ◽

Xiaofei Zheng ◽

Tingting Gai ◽

Zhiyong Wei ◽

Shu-Sheng Zhang

Keyword(s):

Breast Cancer ◽

Escherichia Coli ◽

Drug Delivery ◽

Cell Membrane ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Targeted Delivery ◽

Membrane System ◽

Multifunctional Nanoparticles ◽

Mcf 7

Here, the co-membrane system of MCF-7 breast cancer cell membrane (MM) and Escherichia coli membrane (EM)-coated Fe3O4/MnO2 multifunctional composite nanoparticles loaded with DOX (Fe3O4/MnO2/MM/EM/D) was used for targeting drug delivery...

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A peptide-targeted delivery system with pH-sensitive amphiphilic cell membrane disruption for efficient receptor-mediated siRNA delivery

Journal of Controlled Release ◽

10.1016/j.jconrel.2008.11.010 ◽

2009 ◽

Vol 134 (3) ◽

pp. 207-213 ◽

Cited By ~ 54

Author(s):

Xu-Li Wang ◽

Rongzuo Xu ◽

Zheng-Rong Lu

Keyword(s):

Cell Membrane ◽

Delivery System ◽

Targeted Delivery ◽

Sirna Delivery ◽

Ph Sensitive ◽

Membrane Disruption ◽

Cell Membrane Disruption ◽

Targeted Delivery System

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Au–Fe3O4 nanoagent coated cell membrane for targeted delivery and enhanced chem/photo therapy

Chemical Communications ◽

10.1039/d1cc03454e ◽

2021 ◽

Vol 57 (81) ◽

pp. 10504-10507

Author(s):

Yingshu Guo ◽

Xiuping Cao ◽

Shusheng Zhang

Keyword(s):

Cell Membrane ◽

Targeted Delivery ◽

Treatment Process ◽

Coated Cell ◽

Membrane Coating

We report the preparation of a Au–Fe3O4 nanoagent cell membrane coating, and the treatment process in cell.

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Synthesis and intensive analysis of antibody labeled single core magnetic nanoparticles for targeted delivery to the cell membrane

Journal of Magnetism and Magnetic Materials ◽

10.1016/j.jmmm.2020.167487 ◽

2021 ◽

Vol 521 ◽

pp. 167487

Author(s):

A.V. Ivanova ◽

A.A. Nikitin ◽

A.N. Gabashvily ◽

D.A. Vishnevskiy ◽

M.A. Abakumov

Keyword(s):

Magnetic Nanoparticles ◽

Cell Membrane ◽

Targeted Delivery

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Analysis of Dynamics Targeting CNT-Based Drug Delivery through Lung Cancer Cells: Design, Simulation, and Computational Approach

Membranes ◽

10.3390/membranes10100283 ◽

2020 ◽

Vol 10 (10) ◽

pp. 283

Author(s):

Nafiseh Sohrabi ◽

Afshar Alihosseini ◽

Vahid Pirouzfar ◽

Maysam Zamani Pedram

Keyword(s):

Drug Delivery ◽

Cell Membrane ◽

Delivery System ◽

Targeted Delivery ◽

Specific Area ◽

Systemic Delivery ◽

Cancer Disease ◽

Magnetic Field Profile ◽

Human Lung Cells ◽

Analysis Of Dynamics

Nowadays, carbon nano (CN) structures and specifically carbon nanotubes (CNTs), because of the nanotube’s nanoscale shape, are widely used in carrier and separation applications. The conjugation of CNTs with polysaccharide, proteins, drugs, and magnetic nanoparticles provides a chance for smart targeting and trajectory manipulation, which are used in the crucial field of life science applications, including for cancer disease diagnostics and treatments. Providing an optimal procedure for delivering a drug to a specific area based on mathematical criteria is key in systemic delivery design. Trajectory guidance and applied force control are the main parameters affected by systemic delivery. Moreover, a better understanding of the tissue parameters and cell membrane molecular behaviour are other factors that can be indirectly affected by the targeted delivery. Both sides are an essential part of successful targeting. The lung is one of the challenging organs for drug delivery inside the human body. It has a large surface area with a thin epithelium layer. A few severe diseases directly involve human lung cells, and optimal and successful drug delivery to the lung for the treatment procedure is vital. In this paper, we studied functionalized CNTs’ targeted delivery via crossing through the lung cell membrane. Molecular dynamics (MD) software simulated all the interaction forces. Mathematical modelling of the cell membrane and proposed delivery system based on the relation of velocity and force has been considered. Dynamics equations for CNTs were defined in the time and frequency domain using control theory methods. The proposed delivery system consists of two main parts: crossing through the cell membrane and targeting inside the cell. For both steps, a mathematical model and a proper magnetic field profile have been proposed. The designed system provides criteria for crossing through the cell membrane within 30 s to 5 min and a translocation profile of 1 to 100 Å.

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Folate-Conjugated Cell Membrane Mimetic Polymer Micelles for Tumor-Cell-Targeted Delivery of Doxorubicin

Langmuir ◽

10.1021/acs.langmuir.8b03693 ◽

2018 ◽

Vol 35 (2) ◽

pp. 504-512 ◽

Cited By ~ 11

Author(s):

Qian Lu ◽

Meijun Yi ◽

Mengchen Zhang ◽

Zhangyu Shi ◽

Shiping Zhang

Keyword(s):

Cell Membrane ◽

Tumor Cell ◽

Targeted Delivery ◽

Polymer Micelles ◽

Membrane Mimetic

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New Applications of Lipid and Polymer-Based Nanoparticles for Nucleic Acids Delivery

Pharmaceutics ◽

10.3390/pharmaceutics13122053 ◽

2021 ◽

Vol 13 (12) ◽

pp. 2053

Author(s):

Adelina-Gabriela Niculescu ◽

Alexandra Cătălina Bîrcă ◽

Alexandru Mihai Grumezescu

Keyword(s):

Immune System ◽

Cell Membrane ◽

Nucleic Acids ◽

Targeted Delivery ◽

Enzymatic Degradation ◽

Genetic Disorders ◽

Naked Dna ◽

Nucleic Acids Delivery ◽

New Applications ◽

Novel Delivery Systems

Nucleic acids represent a promising lead for engineering the immune system. However, naked DNA, mRNA, siRNA, and other nucleic acids are prone to enzymatic degradation and face challenges crossing the cell membrane. Therefore, increasing research has been recently focused on developing novel delivery systems that are able to overcome these drawbacks. Particular attention has been drawn to designing lipid and polymer-based nanoparticles that protect nucleic acids and ensure their targeted delivery, controlled release, and enhanced cellular uptake. In this respect, this review aims to present the recent advances in the field, highlighting the possibility of using these nanosystems for therapeutic and prophylactic purposes towards combatting a broad range of infectious, chronic, and genetic disorders.

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Targeted gene silencing in vivo by platelet membrane–coated metal-organic framework nanoparticles

Science Advances ◽

10.1126/sciadv.aaz6108 ◽

2020 ◽

Vol 6 (13) ◽

pp. eaaz6108 ◽

Cited By ~ 14

Author(s):

Jia Zhuang ◽

Hua Gong ◽

Jiarong Zhou ◽

Qiangzhe Zhang ◽

Weiwei Gao ◽

...

Keyword(s):

Cell Membrane ◽

Gene Silencing ◽

Targeted Delivery ◽

Metal Organic Framework ◽

Xenograft Model ◽

Coated Metal ◽

Wide Range ◽

Metal Organic ◽

Organic Framework

Small interfering RNA (siRNA) is a powerful tool for gene silencing that has been used for a wide range of biomedical applications, but there are many challenges facing its therapeutic use in vivo. Here, we report on a platelet cell membrane–coated metal-organic framework (MOF) nanodelivery platform for the targeted delivery of siRNA in vivo. The MOF core is capable of high loading yields, and its pH sensitivity enables endosomal disruption upon cellular uptake. The cell membrane coating provides a natural means of biointerfacing with disease substrates. It is shown that high silencing efficiency can be achieved in vitro against multiple target genes. Using a murine xenograft model, significant antitumor targeting and therapeutic efficacy are observed. Overall, the biomimetic nanodelivery system presented here provides an effective means of achieving gene silencing in vivo and could be used to expand the applicability of siRNA across a range of disease-relevant applications.

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