Camellia sinensis fruit peel extract inhibits angiogenesis and ameliorates obesity induced by high-fat diet in rats

Abstract Pomegranate peel is an agro-industrial residue obtained after fruit processing with high total polyphenol (TP) content, making it an attractive by-product for its reuse. Pomegranate peel extract (PPE) and its bioactive compounds have shown positive effects on obesity models. Effects on favouring mitochondrial biogenesis and function have also been described. However, once phenolic compounds are extracted, their stability can be affected by diverse factors. Microencapsulation could improve PPE stability, allowing its incorporation into functional foods. Nevertheless, studies on the potential biological effects of PPE microparticles (MPPE) in obesity models are lacking. This study aims to evaluate the effect of MPPE on BAT mitochondrial structure and function and metabolic alterations related to obesity in mice fed a high-fat diet (HFD). PPE was microencapsulated by spray drying using inulin (IN) as a wall material and physically-chemically characterized. Eight-week-old male C57BL/6J mice (n=40) were randomly distributed into five groups: control diet (CD), HFD, HFD+IN, HFD+PPE (50 mg/kg/d TP), and HFD+MPPE (50 mg/kg/d TP), for 14 weeks. A glucose tolerance test and indirect calorimetry were conducted. Blood and adipose tissue samples were obtained. MPPE supplementation prevented HFD-induced body weight gain (p<0.001), fasting glycemia (p=0.007), and total cholesterol rise (p=0.001). MPPE resulted in higher BAT mitochondrial complex IV activity (p=0.03) and prevented HFD-induced mitochondrial cristae alteration (p=0.02). In conclusion, MPPE prevented HFD-induced excessive body weight gain and associated metabolic disturbances, potentially by activating complex IV activity and preserving mitochondrial cristae structure in BAT in mice fed with an HFD.

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Effect of Rambutan Fruit Peel Extract on Total Sperm Counts of Wistar Rats With Obesity

Biota ◽

10.20414/jb.v13i1.240 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Kerin Victoria Sipahutar ◽

Yudhi Nugraha ◽

Cut Fauziah

Keyword(s):

Wistar Rats ◽

Semen Quality ◽

Sperm Count ◽

Control Group ◽

Antioxidant Compounds ◽

P Value ◽

High Fat ◽

Fruit Peel ◽

Group I ◽

Peel Extract

Obesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group, subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW. Keywords: Fruit Peel Rambutan Extract; ; ;

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Preventive Effect of Citrus aurantium Peel Extract on High-Fat Diet-Induced Non-alcoholic Fatty Liver in Mice

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b18-00702 ◽

2019 ◽

Vol 42 (2) ◽

pp. 255-260 ◽

Cited By ~ 9

Author(s):

Hyoung-Yun Han ◽

Sung-Kwon Lee ◽

Bong-Keun Choi ◽

Dong-Ryung Lee ◽

Hae Jin Lee ◽

...

Keyword(s):

Fatty Liver ◽

High Fat Diet ◽

Citrus Aurantium ◽

Preventive Effect ◽

High Fat ◽

Alcoholic Fatty Liver ◽

Peel Extract

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Theaflavin Synthesized in a Selective, Domino-Type, One-Pot Enzymatic Biotransformation Method with Camellia sinensis Cell Culture Inhibits Weight Gain and Fat Accumulation to High-Fat Diet-Induced Obese Mice

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b16-00284 ◽

2016 ◽

Vol 39 (8) ◽

pp. 1347-1352 ◽

Cited By ~ 6

Author(s):

Masumi Takemoto ◽

Hiroaki Takemoto ◽

Ryoyasu Saijo

Keyword(s):

Cell Culture ◽

Weight Gain ◽

Camellia Sinensis ◽

High Fat Diet ◽

Obese Mice ◽

High Fat ◽

One Pot ◽

Fat Accumulation

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Protective and Curative Antiobesity Potential of Lemon Peel Extract in Rats Fed on High Fat Diet: Mechanism of Action

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2016/26651 ◽

2016 ◽

Vol 12 (4) ◽

pp. 1-17 ◽

Cited By ~ 1

Author(s):

Magda Ezz ◽

Azza Atef ◽

Nagwa Hassanein ◽

Zeinab Badr

Keyword(s):

Mechanism Of Action ◽

High Fat Diet ◽

High Fat ◽

Lemon Peel ◽

Peel Extract

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High-fat diet effects on the prostatic adenocarcinoma model and jaboticaba peel extract intake: protective response in metabolic disorders and liver histopathology

Nutrition and Cancer ◽

10.1080/01635581.2019.1684526 ◽

2019 ◽

Vol 72 (8) ◽

pp. 1366-1377 ◽

Cited By ~ 1

Author(s):

Ellen Nogueira-Lima ◽

Celina de Almeida Lamas ◽

Andressa Mara Baseggio ◽

Jéssica Stephany Fernandes do Vale ◽

Mário Roberto Maróstica Junior ◽

...

Keyword(s):

High Fat Diet ◽

Metabolic Disorders ◽

Prostatic Adenocarcinoma ◽

High Fat ◽

Protective Response ◽

Liver Histopathology ◽

Peel Extract

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Effect of Kepok Banana Peel Extract (Musa spp.) on the Number of Pituitary Basophil Cells in Rats (Rattus norvegicus) with High-Fat Diet

E3S Web of Conferences ◽

10.1051/e3sconf/202015101063 ◽

2020 ◽

Vol 151 ◽

pp. 01063

Author(s):

Nora Usrina ◽

Muslim Akmal ◽

Rinidar Rinidar ◽

Mustafa Sabri ◽

Gholib Gholib

Keyword(s):

Rattus Norvegicus ◽

High Fat Diet ◽

Household Consumption ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Banana Peel ◽

High Fat ◽

Group 3 ◽

Peel Extract

Banana peels are the outer envelopes of banana fruits as the by-product of household consumption and banana processing. Kepok banana peel contains bioactive compounds that function as antioxidants which reduce the effects of free radicals. This research was conducted to determine the effect of giving Kepok banana peel extract on the number of basophilic cells in rats with a high-fat diet. The study used 25 male rats aged 2.5-3 months old, which alloted into 5 groups with 5 rats each. The first group was fed on standard feed (K1, positive control), while the second group given high-fat diet (K2, negative control). The rats in group 3, 4, and 5 were given high-fat diet + vitamin C (K3), high-fat diet + 100 mg/kg BW banana peel extract (K4), and high-fat diet + 200 mg/ kg BW banana peel extract (K5), respectively. A sample of the pituitary gland was collected after 60 days of the treatment. The data were analyzed using one way analysis of variance (ANOVA) followed by the Duncan test. The results of this study indicated that the administration of Kepok banana peel extract at a dose of 100 mg/kg BW can maintain the number of basophilic cells, whereas at dose of 200 mg/kg BB has the potential to reduce the number of basophilic cells in rats fed high-fat feed.

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Prostatic Adenocarcinoma Progression Delay After Brazilian Berry Extract Intake in Transgenic Mice (Tramp) Submitted to a High- Fat Diet (P05-016-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz030.p05-016-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Cited By ~ 1

Author(s):

Valeria Cagnon ◽

Ellen Lima ◽

Celina Lamas ◽

Andressa Baseggio ◽

Larissa Kido ◽

...

Keyword(s):

High Fat Diet ◽

Diet Group ◽

Control Group ◽

Standard Diet ◽

High Fat ◽

Well Differentiated ◽

Diet Intake ◽

Old Mice ◽

Tramp Mice ◽

Peel Extract

Abstract Objectives Brazilian berries, such as Myrciaria jaboticaba (Vell.) Berg, present a high polyphenol concentration in the peel, showing an antioxidative property. The aim herein was to evaluate the antiangiogenic, antioxidant and proliferative effects of the Jaboticaba peel extract (patent BR 1020170054624) in early adenocarcinoma development in association with high-fat diet intake Methods Tramp mice were divided into 5 groups: Control group 8 (C8): 8 week-old mice; Control group 16 (C16): 16 week-old mice, standard diet; High-fat diet group (CH16): 16 week-old mice, high-fat diet; Jaboticaba standard diet group (JC): 16 week-old mice, standard diet and Jaboticaba intake; Jaboticaba high-fat diet group (HF): 16 week-old mice, high-fat diet and Jaboticaba intake. The 5.8 g Jaboticaba/Kg/body weight dose was administered five days per week for 2 months. The prostate was evaluated for proliferative, antiangiogenic and antioxidative markers, using morphology, immunohistochemistry and Western Blotting analyses. Results The prostate showed increased high grade prostatic intraepithelial neoplasia (HGPIN) and well-differentiated adenocarcinoma in the CH16 group. The Jaboticaba peel (JH group) led to decreased HGPIN. In both the JC and JH groups, a frequency increase of healthy prostatic epithelium was verified. A well-differentiated adenocarcinoma decrease was seen in the JC group. PCNA showed an increase in the CH16 group and a decrease in the JH group. VEGF had an increase in the CH16 group and a decrease after Jaboticaba peel extract intake. Catalase, SOD2, GR and 4HNE showed an increase in the CH16 group and all these molecules presented a decrease after Jaboticaba peel intake in the JH group. The TGFα protein level increased in the C16 and CH16 groups and decreased in the JC and JH groups. Conclusions To conclude, the high-fat diet intake intensified the severity of prostatic lesions. The Jaboticaba peel extract was effective in delaying prostatic adenocarcinoma progression, when administered at the early grades of cancer and considering the lesion severity. Jaboticaba peel intake showed antiangiogenic and antioxidant effects in the prostate, especially, after high-fat diet intake in Tramp mice, indicating a possible coadjuvant role of this natural compound in prostatic cancer therapy. Funding Sources Fapesp 18 045797.

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