scholarly journals Prostatic Adenocarcinoma Progression Delay After Brazilian Berry Extract Intake in Transgenic Mice (Tramp) Submitted to a High- Fat Diet (P05-016-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Valeria Cagnon ◽  
Ellen Lima ◽  
Celina Lamas ◽  
Andressa Baseggio ◽  
Larissa Kido ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Well Differentiated ◽  
Diet Intake ◽  
Old Mice ◽  
Tramp Mice ◽  
Peel Extract

Abstract Objectives Brazilian berries, such as Myrciaria jaboticaba (Vell.) Berg, present a high polyphenol concentration in the peel, showing an antioxidative property. The aim herein was to evaluate the antiangiogenic, antioxidant and proliferative effects of the Jaboticaba peel extract (patent BR 1020170054624) in early adenocarcinoma development in association with high-fat diet intake Methods Tramp mice were divided into 5 groups: Control group 8 (C8): 8 week-old mice; Control group 16 (C16): 16 week-old mice, standard diet; High-fat diet group (CH16): 16 week-old mice, high-fat diet; Jaboticaba standard diet group (JC): 16 week-old mice, standard diet and Jaboticaba intake; Jaboticaba high-fat diet group (HF): 16 week-old mice, high-fat diet and Jaboticaba intake. The 5.8 g Jaboticaba/Kg/body weight dose was administered five days per week for 2 months. The prostate was evaluated for proliferative, antiangiogenic and antioxidative markers, using morphology, immunohistochemistry and Western Blotting analyses. Results The prostate showed increased high grade prostatic intraepithelial neoplasia (HGPIN) and well-differentiated adenocarcinoma in the CH16 group. The Jaboticaba peel (JH group) led to decreased HGPIN. In both the JC and JH groups, a frequency increase of healthy prostatic epithelium was verified. A well-differentiated adenocarcinoma decrease was seen in the JC group. PCNA showed an increase in the CH16 group and a decrease in the JH group. VEGF had an increase in the CH16 group and a decrease after Jaboticaba peel extract intake. Catalase, SOD2, GR and 4HNE showed an increase in the CH16 group and all these molecules presented a decrease after Jaboticaba peel intake in the JH group. The TGFα protein level increased in the C16 and CH16 groups and decreased in the JC and JH groups. Conclusions To conclude, the high-fat diet intake intensified the severity of prostatic lesions. The Jaboticaba peel extract was effective in delaying prostatic adenocarcinoma progression, when administered at the early grades of cancer and considering the lesion severity. Jaboticaba peel intake showed antiangiogenic and antioxidant effects in the prostate, especially, after high-fat diet intake in Tramp mice, indicating a possible coadjuvant role of this natural compound in prostatic cancer therapy. Funding Sources Fapesp 18 045797.

Aerobic exercise regulating expression of ERá and ERâ in prostate to prevent benign prostatic hyperplasia of obesity mice

2019 ◽  
Author(s):  
Xiangyun Liu ◽  
Jiulin Xiang ◽  
Kai Li ◽  
Ru Wang ◽  
Qin Yang
Keyword(s):  
Aerobic Exercise ◽  
High Fat Diet ◽  
Scientific Evidence ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Mice Model ◽  
High Fat ◽  
Prostate Weight ◽  
Diet And Exercise

High-fat-diet (HFD) induced BPH mice model used to examine how aerobics exercise prevents the development of BPH and try to find the mechanism. Materials and Methods: C57BL/6J mice were randomized into three groups for a 6-week intervention: Control group (Co, n = 8) and Diet group (Di, n = 8) fed standard diet, and Exercise and diet group fed standard diet meanwhile run on a treadmill at 12m/min, 30 min/day, 6 days/week (Ex+Di, n = 8). Body weight (BW), fat, prostate weight (PW), prostate index (PI), Estradio(E2), Testosterona (T) and estrogen receptor(ER) were detected. Results: The Di had a higher BW, fat, PW and PI, compared to Co and Ex+Di. BW and PI in Ex+Di significantly lower compared to Di, thus running prevented increase in BW and PI. Although, E2 and T in Di and Ex+Di haven’t seen anything statistically significant, compared with Co. However, ERá in Ex+Di (0.039±0.012 ng/ml) was significant reduced (P less than 0.05) compared with Di (0.046±0.011 ng/ml); Compared with Di (0.032±0.009 ng/ml), ERâ in Ex+Di (0.039±0.011 ng/ml) was significant reduced (P less than 0.05). Conclusion: Obesity is an important risk factor for the development of BPH. It may also provide scientific evidence to promote weight loss and aerobic exercise beneficial adjuvant therapies for BPH.

scholarly journals High-Fat Diet Increases HMGB1 Expression and Promotes Lung Inflammation in Mice Subjected to Mechanical Ventilation

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ana Beatriz Farias de Souza ◽  
Máira Tereza Talma Chírico ◽  
Christiane Teixeira Cartelle ◽  
Guilherme de Paula Costa ◽  
André Talvani ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
High Fat Diet ◽  
Lung Inflammation ◽  
Diet Group ◽  
The Other ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Hmgb1 Expression ◽  
Mechanical Ventilation Group

This study aims to evaluate the effects of a high-fat diet and mechanical ventilation on the pulmonary and systemic inflammatory response in C57BL/6 mice. Male C57BL/6 mice were divided into two groups: one received a standard diet, and the other received a high-fat diet. After 10 weeks, the groups were further divided into two groups each: control group (CG), mechanical ventilation group (MVG), diet group (DG), and diet mechanical ventilation group (DMVG). MVG and DMVG underwent mechanical ventilation for 60 minutes. All animals were euthanized for subsequent analysis. Animals receiving a high-fat diet presented higher body mass, adipose index, and greater adipocyte area. In the lung, the expression of HMGB1 was greater in DG and DMVG than in CG and MVG. CCL2 and IL-22 levels in MVG and DMVG were increased compared to those in CG and DG, whereas IL-10 and IL-17 were decreased. Superoxide dismutase activity was higher in MVG and DMVG than in CG. Catalase activity was lower in DG than in CG, and in MV groups, it was lower than that in CG and DG. MV and obesity promote inflammation and pulmonary oxidative stress in adult C57BL/6 mice.

scholarly journals Identification of Active Compounds of Kepok Banana Peel and the Effect on Testosterone Concentration in Male Rats with High-Fat Diet

2020 ◽  
Vol 151 ◽  
pp. 01026 ◽  
Author(s):  
Baidillah Zulkifli ◽  
Muslim Akmal ◽  
Sri Wahyuni ◽  
Tongku N. Siregar ◽  
Gholib Gholib
Keyword(s):  
Blood Serum ◽  
High Fat Diet ◽  
Diet Group ◽  
Male Rats ◽  
Banana Peel ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Fat ◽  
Testosterone Concentration ◽  
Peel Extract

Our body needs an adequate supply of antioxidants to maintain physiological functions. Antioxidant compounds are found in natural plants, including inside of kepok bananas peel. This research was conducted to determine the potential of kepok banana peels to maintain testosterone concentration in male rats (Rattus norvegicus) with a high-fat diet. This study used 20 male rats 2.5-3 months old, which grouped into 5 groups: normal group given standard feed (K1), negative control group given high-fat diet (K2), positive control group given high-fat diet and simvastatin (K3), high-fat diet group and banana peel extract administered at 100 mg/kg BW (K4), and high-fat diet group and banana peel extract administered at 200 mg/ kg BW (K5). The blood serum was collected after 60 days of the treatment. The blood serum testosterone levels were measured by enzyme-linked immunosorbent assay (ELISA) method at a wavelength of 450 nm. The major findings of this study were that there is a significant difference between testosterone concentrations by the K4 group compared to K2 and K5 groups (p<0,05). The result suggests that the dose 100 mg/kg BW of banana peel extract has the potential to maintain the testosterone concentration in male rats with a high-fat diet.

scholarly journals PO-043 The Effects of One-Time High Intensity Intermittent Training on Expression of LC3 Gene in Rats’ White Adipose Tissue

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Qishu Zhou ◽  
Chunyu Liang ◽  
Yafei Li ◽  
Yi Yan
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Intensity ◽  
High Fat Diet ◽  
Intermittent Exercise ◽  
Diet Group ◽  
Control Group ◽  
High Fat ◽  
Subcutaneous White Adipose Tissue ◽  
White Fat

Objective  To investigate the effect of one-time high-intensity intermittent exercise in white fat autophagy in obese rats and provide a theoretical basis of the molecular mechanism of exercise fat loss. Methods  Eighteen male 3-weeks-old rats were selected and divided into control group fed with normal diet (C), high-fat diet group fed with high fat diet (H). After 16 weeks, there were twelve obesity rats that divided into diet group (HS) and exercise group (HE). The other six control group rats of 19 weeks age were used as the standard (CS group). OE group did the high intensity intermittent exercise once. The CS group and the CS group were kept quietly. Three groups were taken subcutaneous white adipose tissue(S) and epididymal white adipose tissue (E) immediately after exercise. Mensurate the expression of LC3 gene in the tissue using the fluorescent quantitative PCR. Results 1. The expression of LC3 mRNA from white fat tissue was different to the tissues, which the expression of epididymal white adipose tissue of each group was higher than that in subcutaneous white adipose tissue (P <0.01). 2. Compared with CS group, the expression of epididymal white fat adipose tissue LC3 mRNA decreased (P<0.01) and the expression of the subcutaneous white adipose tissue increased from HS group (P <0.05). 3. Compared with OS group, the expression of epididymal white fat adipose tissue LC3 mRNA decreased (P<0.05) and the expression of subcutaneous white adipose tissue decreased from OS group. Conclusions The expression of LC3mRNA in epididymal white fat adipose tissue of rats was significantly higher than that of subcutaneous white fat. The changes of LC3mRNA expression of adipose tissue caused by high-fat diet have tissue differences. One-time high-intensity intermittent exercise can reduce the expression of LC3mRNA in fat tissue of obese rats. Its regulatory mechanism needs to be further studied.

scholarly journals Effect of febuxostat on biochemical parameters of hyperlipidemia induced by a high-fat diet in rabbits

2019 ◽  
Vol 97 (7) ◽  
pp. 611-622 ◽  
Author(s):  
Mohammed M. Heikal ◽  
Ahmed A. Shaaban ◽  
Wagdi F. Elkashef ◽  
Tarek M. Ibrahim
Keyword(s):  
Xanthine Oxidase ◽  
High Fat Diet ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Oxidase Inhibitor ◽  
Diet Group ◽  
Control Group ◽  
High Fat ◽  
Xanthine Oxidase Inhibitor ◽  
Anti Inflammatory

Febuxostat, a highly potent xanthine oxidase inhibitor with an antioxidant effect, inhibits elevated xanthine oxidase, leading to reduction of reactive oxygen species and oxidative stress, the main causes of vascular inflammation in hyperlipidemia. The aim of this study was to test the potential antioxidant and anti-inflammatory effects of febuxostat and (or) stopping a high-fat diet on the biochemical parameters in rabbits with hyperlipidemia induced by a high-fat diet. Male New Zealand rabbits were distributed into 3 groups: a normal control group fed standard chow for 12 weeks and 2 other groups fed a high-fat diet with 1% cholesterol for 8 weeks, and then shifted to standard chow for 4 weeks. During the last 4 weeks, one high-fat diet group received 0.5% carboxymethyl cellulose, whereas the other group was treated with febuxostat (2 mg/kg per day p.o.). Febuxostat significantly lowered low-density lipoprotein cholesterol (“bad” cholesterol) compared to the untreated group (high-fat diet group). Febuxostat also displayed a potent anti-inflammatory and antioxidant activity by decreasing serum levels of lipid peroxidation index, proinflammatory cytokines, and enhancing antioxidant enzyme activity. Stopping the hyperlipidemic diet in the high-fat diet group did not show improvement. These findings indicate the antioxidant and anti-inflammatory effects of febuxostat that may be common mechanisms of the anti-hyperlipidemic effect of this drug. Stopping a hyperlipidemic diet without treatment is not sufficient once injury has occurred.

Molecular factors involved in the hypolipidemic- and insulin-sensitizing effects of a ginger (Zingiber officinale Roscoe) extract in rats fed a high-fat diet

2017 ◽  
Vol 42 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Natalia de las Heras ◽  
María Valero-Muñoz ◽  
Beatriz Martín-Fernández ◽  
Sandra Ballesteros ◽  
Antonio López-Farré ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Plasma Levels ◽  
High Fat Diet ◽  
Tissue Growth ◽  
Collagen I ◽  
Metabolic Effects ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Glucose Transporter 2

Hypolipidemic and hypoglycemic properties of ginger in animal models have been reported. However, information related to the mechanisms and factors involved in the metabolic effects of ginger at a hepatic level are limited. The aim of the present study was to investigate molecular factors involved in the hypoglycemic and hypolipidemic effects of a hydroethanolic ginger extract (GE) in the liver of rats fed a high-fat diet (HFD). The study was conducted in male Wistar rats divided into the following 3 groups: (i) Rats fed a standard diet (3.5% fat), the control group; (ii) rats fed an HFD (33.5% fat); and (iii) rats fed an HFD treated with GE (250 mg·kg−1·day−1) for 5 weeks (HFD+GE). Plasma levels of glucose, insulin, lipid profile, leptin, and adiponectin were measured. Liver expression of glycerol phosphate acyltransferase (GPAT), cholesterol 7 alpha-hydroxylase, peroxisome proliferator-activated receptors (PPAR), PPARα and PPARγ, glucose transporter 2 (GLUT-2), liver X receptor, sterol regulatory element-binding protein (SREBP1c), connective tissue growth factor (CTGF), and collagen I was measured. Data were analyzed using a 1-way ANOVA, followed by a Newman−Keuls test if differences were noted. The study showed that GE improved lipid profile and attenuated the increase of plasma levels of glucose, insulin, and leptin in HFD rats. This effect was associated with a higher liver expression of PPARα, PPARγ, and GLUT-2 and an enhancement of plasma adiponectin levels. Furthermore, GE reduced liver expression of GPAT, SREBP1c, CTGF, and collagen I. The results suggest that GE might be considered as an alternative therapeutic strategy in the management of overweight and hepatic and metabolic−related alterations.

scholarly journals Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis-Induced NC/Nga Mice under High-Fat Diet Conditions

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Ho-Yeol Cha ◽  
Sang-hyun Ahn ◽  
Jin-Hong Cheon ◽  
In-Sik Park ◽  
Jin-Tack Kim ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Morphological Changes ◽  
Skin Lesions ◽  
Control Group ◽  
Therapeutic Effects ◽  
High Fat ◽  
Coptidis Rhizoma ◽  
Inflammatory Regulation ◽  
Th2 Differentiation ◽  
Old Mice

This study investigated the preventive therapeutic effects of Hataedock (HTD) treatment on inflammatory regulation and skin protection in AD-induced NC/Nga mice under high-fat diet conditions. Before inducing AD, the extract ofCoptidis RhizomaandGlycyrrhiza uralensiswas administered orally to the 3-week-old mice. After that, AD-like skin lesions were induced by applying DNFB. All groups except the control group were fed a high-fat diet freely. We identified the effects of HTD on morphological changes, cytokine release and the induction of apoptosis through histochemistry, immunohistochemistry, and TUNEL assay. HTD downregulated the levels of IL-4 and PKC but increased the levels of LXR. HTD also suppressed the mast cell degranulation and release of MMP-9, Substance P. The levels of TNF-α, p-IκB, iNOS, and COX-2 were also decreased. The upregulation of inflammatory cell’s apoptosis is confirmed by our results as increase of apoptotic body and cleaved caspase-3 and decrease of Bcl-2. HTD also reduced edema, angiogenesis, and skin lesion inflammation. Our results indicate HTD suppresses various inflammatory response on AD-induced mice with obesity through the regulation of Th2 differentiation and the protection of lipid barrier. Therefore, HTD could be used as an alternative and preventive therapeutic approach in the management of AD.

THE EFFECT OF A HIGH-FAT DIET ON GLUCOSE, INSULIN SENSITIVITY AND PLASMA INSULIN IN RATS

1968 ◽  
Vol 42 (4) ◽  
pp. 489-494 ◽  
Author(s):  
E. BLÁZQUEZ ◽  
C. LOPEZ QUIJADA
Keyword(s):  
Plasma Glucose ◽  
High Fat Diet ◽  
Plasma Insulin ◽  
Diet Group ◽  
Insulin Concentration ◽  
Control Group ◽  
High Fat ◽  
Plasma Insulin Concentration ◽  
The Mean

SUMMARY The influence of the diet on the levels of insulin was studied in rats on a high-fat diet. Plasma and glucose insulin concentrations of a control group and of rats on a high-fat diet were compared, and so was the insulin concentration in the pancreas of the two groups. The mean plasma insulin concentration in the control group was 40 μ-u./ml. and that of insulin extracted from the pancreas was 2·5 μg./100 mg. tissue; plasma glucose was 156 mg./100 ml. The animals fed on a high-fat diet showed diabetic features. The mean plasma insulin level was 9 μ-u./ml., and plasma glucose increased to 210 mg./100 ml. The insulin concentration in the pancreas was not significantly different from that in the controls. In vitro the epididymal fat and the diaphragm of the high-fat-diet group were less sensitive to insulin than the same tissues in the control group.

scholarly journals Regulating AMPKα and insulin level by Vinegar, Swimming and Refeeding on High-Fat Diet Rats to Rebuild Lipid Homeostasis

2020 ◽  
Author(s):  
Yuan Yang ◽  
Feng Zhang ◽  
Xiao Xiao ◽  
Chunlian Ma ◽  
Hua Liu ◽  
...  
Keyword(s):  
Weight Gain ◽  
High Fat Diet ◽  
Insulin Level ◽  
Diet Group ◽  
Normal Diet ◽  
Lipid Homeostasis ◽  
Control Group ◽  
High Fat ◽  
Better Regulation ◽  
Rice Vinegar

AbstractOur aims were to explore the effects of dietary and behavior interventions on lipometabolism caused by unhealthy high-fat diet and the best method to rebuild lipid homeostasis of this lifestyle. Apart from normal diet rats, 34 rats were fed with high-fat emulsion for 4 weeks before being divided into 4 groups and intervened for another 4 weeks. 8 of them were classified into high-fat control group and 9 were sorted into high-fat diet with rice vinegar group. Meanwhile, 10 were put into high-fat diet with swimming group and 7 were just for refeeding normal diet group. Then the data of body weight was recorded and analyzed. Serum, pancreas, liver, cardiac tissues and epididymis adipose were sampled as required. Indexes of serum were tested by kits. AMPKα, HNF1α, CTRP6 from tissues were detected by western blot. According to our experiments, Swimming and refeeding groups reflected a better regulation on lipid homeostasis mainly by up-regulating the expression of pancreas AMPKα. To be more specific, the refeeding rats showed lower T-CHO (P<0.001) and LDL-C (P<0.05), but higher weight gain (P<0.001),insulin level (P<0.01)and pancreas AMPKα (P<0.01)than high-fat control rats. Compared with rats experimented by swimming or rice vinegar, they showed higher weight gain (P<0.001),insulin level (P<0.01)and HNF1α, but lower of CTRP6. In summary, refeeding diet functioned better in regulating the lipometabolic level after high-fat diet. Whatever approach mentioned above we adopted to intervene, the best policy to keep the balance of lipid homeostasis is to maintain a healthy diet.

scholarly journals Effects of high-fat diet on the epithelial-mesenchymal transition of respiratory tract through the glyoxylic acid cycle of pulmonary microbes and the intervention of saturated hydrogen

2020 ◽  
Author(s):  
Xiangjie Qiu ◽  
Ousman Bajinka ◽  
Lili Wang ◽  
Guojun Wu ◽  
Yurong Tan
Keyword(s):  
Respiratory Tract ◽  
High Fat Diet ◽  
Epithelial Mesenchymal Transition ◽  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
Glyoxylic Acid ◽  
Diet Group ◽  
Control Group ◽  
High Fat ◽  
Mesenchymal Transition

Abstract Background High fat diet is extensively studied to be associated with trending metabolic diseases. In addition to type 2 diabetes and hypertension, high fat diet is strongly associated with asthma and other respiratory diseases among children however, the pathogenicity regarding these pulmonary diseases begs for extensive research. This study investigated the mechanism of the epithelial-mesenchymal transition of respiratory tract, induced by changes in lung microecology with the intake of high-fat diet. 80 five-week-old C57BL6/J male mice were randomly divided into normal control group, normal hydrogen group, high-fat group and high-fat hydrogen group, making 20 mice in each group. The weight of the mice were measured on weekly basis. 6 mice from each group were executed at every second week. Blood sample was collected for lipid testing, lung tissues were collected for 16SrRNA gene sequencing, HE staining, immunofluorescence and quantitative real-time PCR (qPCR).Results Compared with the normal diet group, mice on the high-fat diet group showed increased inflammatory cell infiltration, decreased expression of e-cadherin (E-cad) and increased expression of Twist. There were significant differences in the composition of bacteria in the lung, and the expression of isocitrate lyase (ICL) gene in Pseudomonas aeruginosa , Staphylococcus aureus and Acinetobacter baumannii , which were significantly associated with asthma were seen with a significant increasing trend. After the treatment of saturated hydrogen, the changes in lung microbial population, lung tissue infiltration of inflammatory cells and the transformation of epithelial stroma caused by high-fat diet were moderately alleviated.Conclusion High fat diet can affect the process of airway epithelial stroma by altering the glyoxylate cycle of pulmonary microbes while the pathological process are alleviated by saturated hydrogen by acting on glyoxylate cycle.

Sign in / Sign up

Export Citation Format

Share Document