Identification of a novel genomic resistance island PmGRI1-STP3 and a SXT/R391 integrative conjugative element in Proteus mirabilis of swine origin in China

The emergence and transmission of novel antimicrobial resistance genes pose a great threat to public health globally. Recently, the plasmid-encoding RND efflux pump TMexCD1-TOprJ1 in Klebsiella pneumoniae was reported to reduce the sensitivity of multiple antimicrobials. Herein, we identified a pandrug-resistant Proteus mirabilis isolate, which harbored the novel tmexCD3-toprJ3 gene cluster located on SXT/R391 ICE. This study expands current knowledge in transfer mechanism of tmexCD1-toprJ1-like gene clusters among P. mirabilis and warrant further genomic epidemiology investigations.

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Complete Genome and Plasmids Sequences of a Clinical Proteus mirabilis Isolate Producing Plasmid Mediated NDM-1 From Italy

Microorganisms ◽

10.3390/microorganisms8030339 ◽

2020 ◽

Vol 8 (3) ◽

pp. 339 ◽

Cited By ~ 1

Author(s):

Ibrahim Bitar ◽

Vittoria Mattioni Marchetti ◽

Alessandra Mercato ◽

Elisabetta Nucleo ◽

Adriano Anesi ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Antibiotic Resistance Genes ◽

Genomic Analysis ◽

Small Plasmid ◽

Genomic Epidemiology ◽

Gene Coding ◽

A Genome ◽

Resistance Island

Background: The spread of carbapenemase genes, such as blaNDM-1, in Proteus mirabilis poses a public health threat. The aim of the study was to characterize the genome and plasmids sequences of an NDM-1-positive strain (IBCRE14), which was isolated in 2019 from a catheterized patient hospitalized in Italy. Methods: Whole genome sequencing (WGS) of IBCRE14 was performed on extracted genomic DNA using Sequel I platform. Genome assembly was performed using “Microbial Assembly”. Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases from the Center for Genomic Epidemiology. Results: IBCRE14 had a genome size of 4,018,329 bp and harboured genes coding for resistance to aminoglycosides (aadA1), phenicol (cat), tetracycline (tetJ), and trimethoprim (dfrA1). A large plasmid (pIB_NDM_1) harboured antibiotic resistance genes against sulphonamide (sul1), trimethoprim (dfrA14), tetracycline (tetB), rifampicin (arr-2), aminoglycosides (aadA1, aph3-VI), and beta-lactams (blaOXA-10, blaNDM-1). Furthermore, a small plasmid (pIB_COL3M) harboured a qnrD1 gene coding for quinolone resistance. Conclusion: The ability to conjugate and the presence of a composite antibiotic resistance island suggests that pIB_NDM_1 could both acquire more resistance genes and easily disseminate. To our knowledge, this is the first report on an untypable plasmid harbouring blaNDM-1 in P. mirabilis, in Italy.

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Identification of Proteus genomic island 2 variants in two clonal Proteus mirabilis isolates with coexistence of a novel genomic resistance island PmGRI1

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkaa215 ◽

2020 ◽

Vol 75 (9) ◽

pp. 2503-2507

Author(s):

Chang-Wei Lei ◽

Tian-Ge Yao ◽

Jia Yan ◽

Bo-Yang Li ◽

Xue-Chun Wang ◽

...

Keyword(s):

Resistance Genes ◽

Proteus Mirabilis ◽

Genomic Island ◽

Genomic Islands ◽

Class 1 Integron ◽

E Coli ◽

Class 1 Integrons ◽

Class 1 ◽

Resistance Island ◽

Integrative And Conjugative Element

Abstract Objectives To characterize the MDR genomic islands (GIs) in Proteus mirabilis isolates. Methods Two P. mirabilis strains (C55 and C74) of chicken origin were subjected to WGS (HiSeq and PacBio) and the MDR GIs were determined. Results P. mirabilis strains C55 and C74 are clonal strains and harbour different Proteus genomic island 2 (PGI2) variants (PGI2-C55 and PGI2-C74). The MDR region of PGI2-C55 is composed of two class 1 integrons, separated by a region containing seven copies of IS26 and eight resistance genes, including blaCTX-M-3 and fosA3. The region in PGI2-C74 is a complete In4-type class 1 integron, harbouring five gene cassettes (dfrA16, blaCARB-2, aadA2, cmlA1 and aadA1). In addition, C55 and C74 carry an SXT/R391 integrative and conjugative element (ICEPmiJpn1), harbouring blaCMY-2, and a novel 50.46 kb genomic resistance island named PmGRI1-C55. PmGRI1-C55 harbours a tyrosine-type recombinase/integrase that might be responsible for the integration of PmGRI1-C55 at the 3′ end of tRNA-Sec. It carries an MDR region derived from Tn2670 that harbours a Tn21 region and carries six resistance genes (catA1, blaTEM-1b, aphA1a, sul2, strA and strB). Blast analysis showed diverse PmGRI1 variants in P. mirabilis and Escherichia coli strains. Conclusions The finding of the two new PGI2 variants highlights that the homologous recombination between shared components of class 1 integrons and transposition by IS26 promote the diversity of MDR regions in PGI2. PmGRI1 is a new GI that carries various resistance genes identified in P. mirabilis and E. coli.

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A Novel SXT/R391 Integrative and Conjugative Element Carries Two Copies of the bla NDM-1 Gene in Proteus mirabilis

mSphere ◽

10.1128/msphere.00588-21 ◽

2021 ◽

Author(s):

Jintao He ◽

Long Sun ◽

Linghong Zhang ◽

Sebastian Leptihn ◽

Yunsong Yu ◽

...

Keyword(s):

Public Health ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Health Concern ◽

Public Health Concern ◽

Content Type ◽

Major Public Health Concern ◽

Integrative Conjugative Element ◽

Horizontal Acquisition ◽

Integrative And Conjugative Element

The occurrence of carbapenemase-producing Proteus mirabilis , especially those strains producing NDM-1 and its variants, is a major public health concern worldwide. The integrative conjugative element (ICE) plays an important role in horizontal acquisition of resistance genes.

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Genomic context of resistance genes within a French clinical MDR Proteus mirabilis: identification of the novel genomic resistance island GIPmi1

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dky126 ◽

2018 ◽

Vol 73 (7) ◽

pp. 1808-1811 ◽

Cited By ~ 13

Author(s):

Eliane Siebor ◽

Claire de Curraize ◽

Catherine Neuwirth

Keyword(s):

Resistance Genes ◽

Proteus Mirabilis ◽

The Novel ◽

Genomic Context ◽

Resistance Island

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Identification of AbaR4 Acinetobacter baumannii resistance island in clinical isolates of blaOXA-23-positive Proteus mirabilis

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz472 ◽

2019 ◽

Vol 75 (3) ◽

pp. 521-525 ◽

Cited By ~ 1

Author(s):

Sophie Octavia ◽

Weizhen Xu ◽

Oon Tek Ng ◽

Kalisvar Marimuthu ◽

Indumathi Venkatachalam ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

Proteus Mirabilis ◽

National Surveillance ◽

Class D ◽

Oxford Nanopore ◽

Carbapenemase Gene ◽

Long Read ◽

Encoding Gene ◽

Resistance Island

Abstract Objectives bla OXA-23 is a class D carbapenemase-encoding gene typical of the Acinetobacter genus. However, its occurrence in the Enterobacteriaceae is uncommon. Here we provide the genome characterization of blaOXA-23-positive Proteus mirabilis. Methods In Singapore, a national surveillance of carbapenem non-susceptible clinical Enterobacteriaceae has enabled the collection of OXA-23 bearing isolates. Three clinical P. mirabilis were whole-genome sequenced using Oxford Nanopore MinION and Illumina platforms. The sequence accuracy of MinION long-read contigs was enhanced by polishing with Illumina-derived short-read data. Results In two P. mirabilis genomes, blaOXA-23 was detected as two copies, present on the chromosome and on a 60018 bp plasmid. blaOXA-23 was associated with the classic Acinetobacter composite transposon Tn2006, bounded by two copies of ISAba1 bracketing the carbapenemase gene. The Tn2006 itself was embedded within an Acinetobacter baumannii AbaR4 resistance island. In the chromosome, the AbaR4 was found integrated into the comM gene, which is also the preferred ‘hotspot’ in A. baumannii. In the plasmid, AbaR4 integrated into a putative colicin gene. Conclusions Our description of an A. baumannii AbaR4 encoding blaOXA-23 in P. mirabilis is to our knowledge the first description of an Acinetobacter resistance island in Proteus and suggests that P. mirabilis may be a reservoir for this class D carbapenemase gene.

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Ultrastructure of periplasmic bodies in gram-negative bacteria

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160753 ◽

1990 ◽

Vol 48 (3) ◽

pp. 640-641

Author(s):

Xie Nianming ◽

Ding Shaoqing ◽

Wang Luping ◽

Yuan Zenglin ◽

Zhan Guolai ◽

...

Keyword(s):

Electron Microscope ◽

Campylobacter Jejuni ◽

Proteus Mirabilis ◽

Shigella Flexneri ◽

Morphological Description ◽

Gram Negative Bacteria ◽

Periplasmic Space ◽

Clostridium Tetani ◽

Gram Negative ◽

Brucella Canis

Perhaps the data about periplasmic enzymes are obtained through biochemical methods but lack of morphological description. We have proved the existence of periplasmic bodies by electron microscope and described their ultrastructures. We hope this report may draw the attention of biochemists and mrophologists to collaborate on researches in periplasmic enzymes or periplasmic bodies with each other.One or more independent bodies may be seen in the periplasmic space between outer and inner membranes of Gram-negative bacteria, which we called periplasmic bodies. The periplasmic bodies have been found in seven species of bacteria at least, including the Pseudomonas aeroginosa. Shigella flexneri, Echerichia coli. Yersinia pestis, Campylobacter jejuni, Proteus mirabilis, Clostridium tetani. Vibrio cholerae and Brucella canis.

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