Epidemiology and molecular characterization of fecal carriage of third-generation cephalosporin-resistant enterobacterales among elderly residents in Japan

We isolated Shiga toxin-producingEscherichia coliO157:H7 strains resistant to third-generation cephalosporins. The resistant strains harboredblaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the investigation and surveillance of enterobacteria with plasmids harboringblaCMY-2.

Download Full-text

Serotype profile and molecular characterization of antimicrobial resistance in non-typhoidal Salmonella isolated from gastroenteritis cases over nine years

Journal of Medical Microbiology ◽

10.1099/jmm.0.061416-0 ◽

2014 ◽

Vol 63 (1) ◽

pp. 66-73 ◽

Cited By ~ 10

Author(s):

Neelam Taneja ◽

Suma B. Appannanavar ◽

Ajay Kumar ◽

Garima Varma ◽

Yashwant Kumar ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Resistance Genes ◽

Salmonella Enterica ◽

Third Generation ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

First Time ◽

Third Generation Cephalosporins

Resistance to third-generation cephalosporins in non-typhoidal Salmonella (NTS) is emerging worldwide. We report the occurrence of extended-spectrum beta-lactamase (ESBL) phenotypes in 53.4 % of NTS isolated over a period of nine years from gastroenteritis cases. ESBL and AmpC co-production was observed in 21 % of the isolates. Occurrence of bla CTX-M-15 and bla CMY-2 resistance genes was observed in 11.6 % and 37 % of the isolates respectively. Overall, Salmonella enterica serovar Senftenberg was the predominant serovar carrying bla CTX-M-15 and bla CMY-2 resistance genes. We report for the first time from India, one isolate each of S. enterica serovar Thompson, S. enterica serovar Infantis and S. enterica serovar Newport, carrying the bla CTX-M-15 gene. We also report for the first time from India, a case of gastroenteritis due to S. enterica serovar Thompson.

Download Full-text

Fecal Carriage and Molecular Characterization of Carbapenemase-Producing Enterobacterales in the Pediatric Population in Qatar

Microbiology Spectrum ◽

10.1128/spectrum.01122-21 ◽

2021 ◽

Author(s):

Andrés Pérez-López ◽

Sathyavathi Sundararaju ◽

Kin Ming Tsui ◽

Hassan Al-Mana ◽

Mohammad Rubayet Hasan ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Molecular Characterization ◽

Genome Sequencing ◽

Arabian Peninsula ◽

Pediatric Population ◽

Molecular Characteristics ◽

Whole Genome ◽

Fecal Carriage ◽

Demographic Patterns

To the best of our knowledge, this is the first study addressing the molecular characteristics of CPE in a pediatric population in Qatar using whole-genome sequencing. Since several countries in the Arabian Peninsula share relatively similar demographic patterns and international links, it is plausible that the molecular characteristics of CPE in children, at least in the middle and eastern parts of the region, are similar to those observed in our study.

Download Full-text

Characterization of a P1-like bacteriophage carrying CTX-M-27 in Salmonella spp. resistant to third generation cephalosporins isolated from pork in China

Scientific Reports ◽

10.1038/srep40710 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 16

Author(s):

Ling Yang ◽

Wan Li ◽

Gui-Ze Jiang ◽

Wen-Hui Zhang ◽

Huan-Zhong Ding ◽

...

Keyword(s):

Potential Role ◽

Generation Cephalosporin ◽

Complete Sequence ◽

Third Generation ◽

Salmonella Spp ◽

Incp Plasmids ◽

Horizontal Spread ◽

Third Generation Cephalosporins

Abstract The aim of this study was to elucidate the epidemiology of third generation cephalosporin resistant Samonella isolates from pork of a slaughterhouse in China and the features of transferable elements carrying bla CTX-M genes. One hundred and twenty-six (7.3%) Salmonella isolates were identified; S. Derby and S. Rissen were the most two prevalent serotypes. Among these isolates 20 (15.8%) were resistant to third generation cephalosporins and nine of them carried bla CTX-M-27. S1-PFGE and replicon typing of bla CTX-M-27-carrying plasmids showed that seven were untypeable plasmids of about 104 Kb and two were IncP plasmids of about 300 Kb. Complete sequence analysis of one PBRT-untypeable plasmid showed it was a P1-like bateriophage, named SJ46, which contained a non-phage-associated region with several mobile elements, including Tn1721, ISEcp1B and IS903D. The other six 104 Kb PBRT-untypeable bla CTX-M-27-carrying plasmids also harboured the same phage-insertion region of SJ46 suggesting that they were the same P1-like bacteriophage. PFGE profiles of the parental strains revealed both potential vertical and horizontal spread of this P1-like bla CTX-M-27-containing element. Additionally, the representative gene of the P1 family bacteriophage, repL, was detected in 19.0% (24/126) of the isolates. This study indicated a potential role of P1-family bacteriophage in capture and spread of antimicrobial resistance in pathogens.

Download Full-text

Comparative genetic characterization of third-generation cephalosporin-resistant Escherichia coli from chicken meat produced by integrated broiler operations in South Korea

Poultry Science ◽

10.3382/ps/pey127 ◽

2018 ◽

Vol 97 (8) ◽

pp. 2871-2879 ◽

Cited By ~ 8

Author(s):

Kwang Won Seo ◽

Yeong Bin Kim ◽

Hye Young Jeon ◽

Suk-Kyung Lim ◽

Young Ju Lee

Keyword(s):

Escherichia Coli ◽

South Korea ◽

Genetic Characterization ◽

Generation Cephalosporin ◽

Chicken Meat ◽

Third Generation

Download Full-text

Synthesis, Characterization of Ceftibuten-Copper(II) Complex and Prediction of Its Biological Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2021.22982 ◽

2020 ◽

Vol 33 (1) ◽

pp. 190-194

Author(s):

Shuchismita Dey ◽

Md. Zakir Sultan ◽

Md. Abdus Salam

Keyword(s):

Antibacterial Activity ◽

Generation Cephalosporin ◽

Salmonella Typhi ◽

In Vitro Test ◽

Third Generation ◽

The Third ◽

Vitro Test ◽

Cephalosporin Antibiotic

Ceftibuten dihydrate, one of the third-generation cephalosporin antibiotic is effectively used in curing several infectious diseases. The complexation of drug with metal may enhance the antibacterial activity. In this work, a new complex of ceftibuten dihydrate with Cu(II) was synthesized, characterized and antibacterial activity is reported. The in vitro test showed that the antibacterial activity of complex of ceftibuten was greatly enhanced against Staphylococcus aureus and Salmonella typhi.

Download Full-text

Comparative genetic characterization of third-generation cephalosporin-resistant Escherichia coliisolated from a layer operation system in Korea

Poultry Science ◽

10.3382/ps/pey513 ◽

2019 ◽

Vol 98 (3) ◽

pp. 1472-1479 ◽

Cited By ~ 1

Author(s):

Kwang Won Seo ◽

Jong Bo Shim ◽

Young Ju Lee

Keyword(s):

Genetic Characterization ◽

Generation Cephalosporin ◽

Third Generation ◽

Operation System

Download Full-text

Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04408-14 ◽

2015 ◽

Vol 59 (5) ◽

pp. 2949-2955 ◽

Cited By ~ 19

Author(s):

Nadia Wohlwend ◽

Andrea Endimiani ◽

Thierry Francey ◽

Vincent Perreten

Keyword(s):

Klebsiella Pneumoniae ◽

Companion Animals ◽

Generation Cephalosporin ◽

Sequence Type ◽

Third Generation ◽

Content Type ◽

Human Infections ◽

Veterinary Hospital ◽

Animal Isolates

ABSTRACTCharacterization of third-generation-cephalosporin-resistantKlebsiella pneumoniaeisolates originating mainly from one human hospital (n= 22) and one companion animal hospital (n= 25) in Bern (Switzerland) revealed the absence of epidemiological links between human and animal isolates. Human infections were not associated with the spread of any specific clone, while the majority of animal infections were due toK. pneumoniaesequence type 11 isolates producing plasmidic DHA AmpC. This clonal dissemination within the veterinary hospital emphasizes the need for effective infection control practices.

Download Full-text