Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

2016 ◽  
Vol 436 ◽  
pp. 41-49 ◽  
Author(s):  
Mi Li ◽  
Xiubin Xiao ◽  
Lianqing Liu ◽  
Ning Xi ◽  
Yuechao Wang
Keyword(s):  
Functional Analysis ◽  
Atomic Force Microscopy ◽  
Membrane Proteins ◽  
Cancer Cells ◽  
Human Cancer ◽  
Force Microscopy ◽  
Human Cancer Cells ◽  
Atomic Force

Study of NSCLC cell migration promoted by NSCLC-Derived Extracellular Vesicle using atomic force microscopy

2021 ◽  
Author(s):  
Shuwei Wang ◽  
Jiajia Wang ◽  
Tuoyu Ju ◽  
Kaige Qu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Extracellular Vesicle ◽  
Cancer Microenvironment ◽  
Biological Processes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cellular Components

Extracellular Vesicles (EVs) secreted by cancer cells have a key role in the cancer microenvironment and progression. Previous studies have mainly focused on molecular functions, cellular components and biological processes...

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

Viscoelastic properties of doxorubicin-treated HT-29 cancer cells by atomic force microscopy: the fractional Zener model as an optimal viscoelastic model for cells

2019 ◽  
Vol 19 (3) ◽  
pp. 801-813 ◽  
Author(s):  
Maricela Rodríguez-Nieto ◽  
Priscila Mendoza-Flores ◽  
David García-Ortiz ◽  
Luis M. Montes-de-Oca ◽  
Marco Mendoza-Villa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Viscoelastic Model ◽  
Zener Model ◽  
Force Microscopy ◽  
Atomic Force ◽  
Fractional Zener Model ◽  
Ht 29

Quantitative Measurements of Intercellular Adhesion Strengths between Cancer Cells with Different Malignancies Using Atomic Force Microscopy

2019 ◽  
Vol 91 (16) ◽  
pp. 10557-10563 ◽  
Author(s):  
Hyonchol Kim ◽  
Kenta Ishibashi ◽  
Kosuke Matsuo ◽  
Atsushi Kira ◽  
Tomoko Okada ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Intercellular Adhesion ◽  
Force Microscopy ◽  
Quantitative Measurements ◽  
Atomic Force

scholarly journals Evaluation of 5-fluorouracil-treated lung cancer cells by atomic force microscopy

2019 ◽  
Vol 11 (39) ◽  
pp. 4977-4982 ◽  
Author(s):  
Xiaolin Jiang ◽  
Ke Ma ◽  
Cuihua Hu ◽  
Mingyan Gao ◽  
Jiashuo Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Side Effect ◽  
Drug Efficacy ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Small Cell Lung ◽  
Force Microscopy ◽  
Atomic Force

The drug efficacy and side-effect of 5-fluorouracil for non-small cell lung cancer cells were studied by atomic force microscopy.

Sign in / Sign up

Export Citation Format

Share Document