Septin homologs cooperating in the Proliferative Stage of Microsporidia Nosema bombycis(Septins cooperating in meront proliferation)

2021 ◽  
pp. 107600
Author(s):  
Jun Huang ◽  
Jie Chen ◽  
Fangyan Liu ◽  
Qiang He ◽  
Yujiao Wu ◽  
...  
Keyword(s):  
Nosema Bombycis ◽  
Proliferative Stage

Pathogenicity and genetic divergence of two isolates of microsporidia Nosema bombycis

2009 ◽  
Vol 31 (11) ◽  
pp. 1121-1126 ◽  
Author(s):  
Min WANG ◽  
Jin-Shan XU ◽  
Lin-Ling WANG ◽  
Xiao-Yan ZHANG ◽  
Ze-Yang ZHOU
Keyword(s):  
Genetic Divergence ◽  
Nosema Bombycis

Transcriptome analysis reveals changes in silkworm energy metabolism during Nosema bombycis infection

2021 ◽  
Vol 174 ◽  
pp. 104809
Author(s):  
Nan Hu ◽  
Zhan-Qi Dong ◽  
Jiang-Qiong Long ◽  
Ning Zheng ◽  
Cong-Wu Hu ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Transcriptome Analysis ◽  
Nosema Bombycis

scholarly journals The role of NbTMP1, a surface protein of sporoplasm, in Nosema bombycis infection

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shiyi Zheng ◽  
Yukang Huang ◽  
Hongyun Huang ◽  
Bin Yu ◽  
Ni Zhou ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Selective Breeding ◽  
Developmental Stages ◽  
Transmembrane Protein ◽  
Surface Protein ◽  
Spore Wall ◽  
Significant Inhibition ◽  
Pcr Analysis ◽  
Immunofluorescence Analysis ◽  
Nosema Bombycis

Abstract Background Nosema bombycis is a unicellular eukaryotic pathogen of the silkworm, Bombyx mori, and is an economic and occupational hazard in the silkworm industry. Because of its long incubation period and horizontal and vertical transmission, it is subject to quarantine measures in sericulture production. The microsporidian life-cycle includes a dormant extracellular phase and intracellular proliferation phase, with the proliferation period being the most active period. This latter period lacks spore wall protection and may be the most susceptible stage for control. Methods In order to find suitable target for the selective breeding of N. bombycis-resistant silkworm strains, we screen highly expressed membrane proteins from the transcriptome data of N. bombycis. The subcellular localization of the candidate protein was verified by Indirect immunofluorescence analysis (IFA) and immunoelectron microscopy (IEM), and its role in N. bombycis proliferation was verified by RNAi. Results The N. bombycis protein (NBO_76g0014) was identified as a transmembrane protein and named NbTMP1. It is homologous with hypothetical proteins NGRA_1734 from Nosema granulosis. NbTMP1 has a transmembrane region of 23 amino acids at the N-terminus. Indirect immunofluorescence analysis (IFA) results suggest that NbTMP1 is secreted on the plasma membrane as the spores develop. Western blot and qRT-PCR analysis showed that NbTMP1 was expressed in all developmental stages of N. bombycis in infected cells and in the silkworm midgut. Downregulation of NbTMP1 expression resulted in significant inhibition of N. bombycis proliferation. Conclusions We confirmed that NbTMP1 is a membrane protein of N. bombycis. Reduction of the transcription level of NbTMP1 significantly inhibited N. bombycis proliferation, and this protein may be a target for the selective breeding of N. bombycis-resistant silkworm strains.

Influence of Cisplatin on Wound Healing—An Experimental Model

1986 ◽  
Vol 95 (2) ◽  
pp. 210-212 ◽  
Author(s):  
Charles M. Stiernberg ◽  
R. Mark Williams ◽  
James A. Hokanson
Keyword(s):  
Wound Healing ◽  
Chemotherapeutic Agents ◽  
Optimal Time ◽  
Subcutaneous Injections ◽  
Treatment Groups ◽  
Cell Counts ◽  
Proliferative Stage ◽  
Significant Difference ◽  
Surgical Staples ◽  
Outbred Mice

Recent clinical studies have shown that adjuvant chemotherapy may improve response rates to treatment for advanced head and neck carcinomas. Given preoperatively, some chemotherapeutic agents adversely affect wound heallng. The specific purpose of this study was to evaluate the Influence of cisplatin on wound healing when it is given preoperatively. Forty-four Swiss outbred mice were divided into control and treatment groups. One week before surgery, the treatment group received cisplatin (2 mg/ kg body weight) by subcutaneous injections on 2 consecutive days. Each control animal was given an equal volume of normal saline. A 1.5 cm transverse incision was made in each animal, and wounds were closed with surgical staples. The mean woundbreaking strength was determined for a minimum of 5 treatment and 5 control mice on postoperative days 6, 10, 13, and 16. Serum creatinine, blood cell counts, and changes in weight were also monitored. Results showed wound strength on postoperative day 10 to be significantly reduced in mice treated with cisplatin (P < 0.05). There was no significant difference for wound strength on any other days and all other variables were simllar between both groups. In conclusion, cisplatin has an adverse effect on wound healing, the peak of which probably occurs during the proliferative stage of wound healing. Further studies are needed to determine the optimal time for surgery after preoperative chemotherapy. All new chemotherapeutic agents, particularly those being considered in a preoperative regimen, should be tested in this manner.

scholarly journals What Is a Solitary Keratoacanthoma? A Benign Follicular Neoplasm, Frequently Associated with Squamous Cell Carcinoma

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1848
Author(s):  
Azusa Ogita ◽  
Shin-ichi Ansai
Keyword(s):  
Elderly Patient ◽  
Follicular Neoplasm ◽  
Histopathological Diagnosis ◽  
Complete Excision ◽  
Exposed Area ◽  
Pale Pink ◽  
Proliferative Stage ◽  
Epithelial Neoplasms ◽  
Residual Tissue ◽  
Careful Investigation

We present histopathological criteria for diagnosing keratoacanthoma (KA). In KA, four histological stages are recognized, which are the early/proliferative stage, well-developed stage, regressing stage and regressed stage. In diagnosing KA, we emphasize that KA consists of the proliferation of enlarged pale pink cells with ground glass-like cytoplasm without nuclear atypia, other than crateriform architecture. KA sometimes exhibits malignant transformation within the lesions. We describe the characteristics of benign and malignant epithelial crateriform tumors that should be differentiated from KA. We also present the data of histopathological diagnosis of lesions clinically diagnosed as KA, its natural course and related lesions after partial biopsy, and incidence of crateriform epithelial neoplasms. Based on these data, we recommend complete excision of the lesion when KA is clinically suspected, especially when the lesion is located on a sun-exposed area of an elderly patient. If complete excision is impossible, partial excision of a sufficient specimen with intact architecture is required. In such a case, however, careful investigation after biopsy will be needed, even if the histopathological diagnosis is KA, because there is some possibility that a conventional SCC lesion remains in the residual tissue.

Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose

2019 ◽  
Vol 118 (5) ◽  
pp. 1511-1518 ◽  
Author(s):  
Viacheslav V. Dolgikh ◽  
Alexander A. Tsarev ◽  
Sergey A. Timofeev ◽  
Vladimir S. Zhuravlyov
Keyword(s):  
Nosema Ceranae ◽  
Nosema Bombycis ◽  
Heterologous Overexpression

Identification and subcellular localization of splicing factor arginine/serine-rich 10 in the microsporidian Nosema bombycis

2020 ◽  
Vol 174 ◽  
pp. 107441
Author(s):  
Zhilin Zhang ◽  
Mingshuai Yao ◽  
Guanyu Zhu ◽  
Yong Chen ◽  
Ying Chen ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Splicing Factor ◽  
Nosema Bombycis
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