Antibacterial activity of Illicium verum essential oil against MRSA clinical isolates and determination of its phyto-chemical components

Gas chromatography/mass spectroscopy analysis was performed to identify the chemical components of three extracted essential oils including thyme, marjoram, and sage. The antibacterial activity of the extracted essential oils against Pseudomonas aeruginosa (ATCC 9027) was investigated using disc diffusion assay, either alone or in combination with standard antibiotics (piperacillin, cefepime, meropenem, gentamicin, and norfloxacin). Results showed that the studied oils exhibited a variety of activities against the tested bacterium. Thyme oil was the most active followed by marjoram oil, whereas sage displayed no activity towards the tested organism. Thyme oil enhanced the antibacterial activity of cell wall targeting antibiotics (piperacillin, cefepime, and meropenem) by more than twofold. Marjoram oil potentiated the activity of all the tested antibiotics except norfloxacin. Sage, despite its inactivity against pseudomonas, synergistically enhanced the activity of piperacillin, meropenem, and gentamicin. Thyme essential oil, containing thymol as a major component (33.6%), exhibited higher activity alone or in combination with antibiotics than marjoram which contained alcoholic terpenes or sage essential oil that contained 1,8-cineole as its major component (29%). The investigated oils, as natural bioactive agents, may be used to enhance the activity of antibiotics towards pseudomonas.

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Antibacterial activity ofThymus pallidusBatt. and determination of the chemical composition of its essential oil

Acta Botanica Gallica ◽

10.1080/12538078.2009.10516160 ◽

2009 ◽

Vol 156 (2) ◽

pp. 303-310 ◽

Cited By ~ 10

Author(s):

Hakima Sqalli ◽

Asmae El Ouarti ◽

Abdellah Farah ◽

Abdeslam Ennabili ◽

Abdellatif Haggoud ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil

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Determination of antibacterial activity of vacuum distillation fractions of lemongrass essential oil

Phytochemistry Reviews ◽

10.1007/s11101-012-9255-3 ◽

2012 ◽

Vol 11 (4) ◽

pp. 405-412 ◽

Cited By ~ 12

Author(s):

Manuel A. Falcão ◽

Ana L. B. Fianco ◽

Aline M. Lucas ◽

Marcos A. A. Pereira ◽

Fernando C. Torres ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Vacuum Distillation ◽

Lemongrass Essential Oil

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Chemical Composition and Antibacterial Activity of Essential Oil from Leaves ofJusticia adhatodaAgainst Methicillian Resistant and Sensitive Strain Along with Their Clinical Isolates

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2016.1260061 ◽

2017 ◽

Vol 20 (1) ◽

pp. 116-122 ◽

Cited By ~ 2

Author(s):

Shipra Shukla ◽

Satisha Hegde ◽

Anil Kumar ◽

Gaurav Chaudhary ◽

S.K. Tewari ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Sensitive Strain ◽

Clinical Isolates

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Chemical Composition and Evaluation of the Antimicrobial Activity of the Of Illicium Verum and the Derivations of Protocatechuic Acid

10.31487/j.gdt.2020.01.03 ◽

2020 ◽

pp. 1-6

Author(s):

Huma Aslam Bhatti ◽

Aqeel Ahmad ◽

Fraha Naz Chohan ◽

Huma Aslam Bhatti ◽

Sara . ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Protocatechuic Acid ◽

Authentic Sample ◽

Spectroscopic Studies ◽

Active Fraction ◽

Dihydroxybenzoic Acid ◽

Illicium Verum

In our present studies we identify the components of essential oil, crude extracts and fraction of Illicium verum, and evaluation of antibacterial activity. We isolated and identified as 3, 4-dihydroxybenzoic acid (protocatechuic acid) through spectroscopic studies and comparison with the data of the authentic sample [1]. The phase MEA itself was emerged as the active fraction comprising antibacterial, antioxidant and nematocidal constituents, including 3, 4-dihydroxybenzoic acid which also possessed antibacterial, Superb antioxidant and nematocidal activities [1].

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Comparison of Chemical Composition between Kuromoji (Lindera umbellata) Essential Oil and Hydrosol and Determination of the Deodorizing Effect

Molecules ◽

10.3390/molecules25184195 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4195

Author(s):

Naoki Nanashima ◽

Maiko Kitajima ◽

Shizuka Takamagi ◽

Miyuki Fujioka ◽

Toshiko Tomisawa

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Isovaleric Acid ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Components ◽

Methyl Mercaptan ◽

Foot Care

Kuromoji (Lindera umbellata) is a tree that grows throughout Japan. The components of kuromoji essential oil have antitumor and aromatherapy effects. However, the composition of the hydrosol, obtained as a by-product of the essential oil process, is unknown. Furthermore, it is unknown whether kuromoji essential oil has a deodorizing effect. Therefore, the purpose of the current study was to compare the chemical composition of kuromoji essential oil and hydrosol, as well as evaluate the deodorizing effect of the former. The chemical composition of samples was evaluated using gas chromatography–mass spectrometry (GC-MS). Additionally, the deodorizing effect of Kuromoji essential oil was investigated with the detector tube method using ammonia, hydrogen sulfide, methyl mercaptan, and isovaleric acid. Linalool was the most abundant component in both the essential oil and hydrosol; however, its proportion was higher in the hydrosol (57.5%) than in the essential oil (42.8%). The hydrosol contained fewer chemical components, but higher proportions of trans-geraniol and ethanol. Moreover, the essential oil eliminated 50% of ammonia and 97.6% or more of isovaleric acid. Interestingly, linalool was soluble in the hydrosol and did not irritate the skin. This suggests that the hydrosol may be an effective foot care product.

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Star anise ( Illicium verum Hook. f.) essential oil: Antioxidant properties and antibacterial activity against Acinetobacter baumannii

Flavour and Fragrance Journal ◽

10.1002/ffj.3498 ◽

2019 ◽

Vol 34 (4) ◽

pp. 260-270 ◽

Cited By ~ 8

Author(s):

Ângelo Luís ◽

Sónia Sousa ◽

Judith Wackerlig ◽

Daniel Dobusch ◽

Ana Paula Duarte ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Acinetobacter Baumannii ◽

Antioxidant Properties ◽

Star Anise ◽

Illicium Verum

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In vitro antimicrobial and cytotoxic activity of Lippia origanoides essential oil against bacteria of potential health concern

10.1101/2021.09.02.458771 ◽

2021 ◽

Author(s):

Edwin Stiven Quiguanás Guarín ◽

Juan Pablo Bedoya Agudelo ◽

Jhon Esteban Lopez-Carvajal ◽

Yuly Andrea Ramírez Tabares ◽

Leonardo Padilla Sanabria ◽

...

Keyword(s):

Antibacterial Activity ◽

Natural Products ◽

Essential Oil ◽

Vero Cells ◽

In Vitro Cytotoxicity ◽

Health Concern ◽

Chemical Components ◽

Potential Health ◽

Major Chemical

Due to the growing resistance they develop of bacteria to drugs, the search for alternatives in natural products is considered important such as Lippia origanoides essential oil. Here, the antibacterial activity of the oil and two of its major chemical components were tested against bacteria of potential health concern. The cytotoxicity of these compounds was evaluated in human erythrocytes and Vero cells. 51 compounds were identified in the LOEO, being terpinen-4-ol, γ-Terpinene, citronellal and thymol the main. LOEO and thymol showed antibacterial activity from 904 μg/mL and 200 μg/mL, respectively. γ-Terpinene did not show activity any concentration tested. LOEO showed hemolysis at concentration of 3000 μg/mL and thymol at 100 μg/mL. LOEO and thymol showed cytotoxicity in the evaluated cell lines at 250 μg/mL and 100 μg/mL, respectively. These compounds have a moderate cytotoxicity so it's considered necessary to study alternatives to reduce the in vitro cytotoxicity of these compounds.

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Analysis of Chemical Components and Antibacterial Activity in Essential Oil of Lantana Flowers (Lantana Camara L)

Biomedika ◽

10.31001/biomedika.v14i1.1164 ◽

2021 ◽

Vol 14 (1) ◽

pp. 19-28

Author(s):

Yulia Shara Sembiring ◽

Cut Fatimah Zuhra

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Antibacterial Activities ◽

Lantana Camara ◽

Disk Diffusion ◽

Diffusion Method ◽

Chemical Components ◽

Oil Composition ◽

Bacterial Strains ◽

Zone Of Inhibition

The purpose of this study was to evaluate the essential oil composition as well as antibacterial activities of essential oil of Lantana camara L flowers against four bacterial strains. Essential oil of Lantana camara L flowers was obtained by hydrodistillation method using Sthal and analyzed by GC-MS. The antibacterial activities of essential oil were tested by using disk diffusion method against four bacterial strains. Results showed 72 compounds for Lantana flowers essential oil, of which caryophyllene (10,87%), davanone (9.84%), a-humulene (7.59%), a-curcumene (3.35%), germacrene D (3.09%), calarene (2.42%), a-muurolene (2.27%), p-cymene (1.79%), 1,8 cineole (1.59%) , δ-cadinene (1.59%), a-copaene (1.12%), nerolidol B (1%) and ß-ocimene (0.54%) were detected as major components. Antibacterial activity assay of Lantana camara L flowers essential oil was examined against four bacterial strains including Gram (+and-) by using disk diffusion agar method. All the tested Gram (+and-) bacterial strains displayed slight to moderate antibacterial activity (7 – 10 mm) against all concentrations of essential oil. The essential oil of Lantana camara L flowers showed remarkable antibacterial activity with inhibition in concentrations of 10% and 20% against the phatogen bacterias Basillus subtilis (9.6 mm; 10.8 mm), Propionibacterium acnes ( 7.6 mm; 8.7 mm), Eschechia coli ( 8.4 mm ; 9.6 mm) and Pseudomonas aeruginosa ( 8.3 mm ; 9.3 mm) showed from zone of inhibition that was formed, zone of inhibition in concentration 20% was bigger than concentration 10%.

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