Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis

ABSTRACT Objectives: To investigate the effects of light and heavy forces with corticotomy on tooth movement rate, alveolar bone response, and root resorption in a rat model. Materials and Methods: The right and left sides of 40 male Wistar rats were randomly assigned using the split-mouth design to two groups: light force with corticotomy (LF) and heavy force with corticotomy (HF). Tooth movement was performed on the maxillary first molars using a nickel-titanium closed-coil spring delivering either 10 g (light force) or 50 g (heavy force). Tooth movement and alveolar bone response were assessed by micro–computed tomography (micro-CT) at day 0 as the baseline and on days 7, 14, 21, and 28. Root resorption was examined by histomorphometric analysis at day 28. Results: Micro-CT analysis showed a significantly greater tooth movement in the HF group at days 7 and 14 but no difference in bone volume fraction at any of the observed periods. Histomorphometric analysis found no significant difference in root resorption between the LF and HF groups at day 28. Conclusions: Heavy force with corticotomy increased tooth movement at days 7 and 14 but did not show any difference in alveolar bone change or root resorption.

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Bone Micro-CT Assessments in an Orchidectomised Rat Model Supplemented withEurycoma longifolia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/501858 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Rosmaliza Ramli ◽

Mohd Fadhli Khamis ◽

Ahmad Nazrun Shuid

Keyword(s):

Trabecular Bone ◽

Bone Microarchitecture ◽

Bone Volume ◽

High Dose ◽

Micro Ct ◽

Sprague Dawley ◽

Elderly Male ◽

Trabecular Bone Microarchitecture ◽

Eurycoma Longifolia ◽

Induced Changes

Recent studies suggested thatEurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects ofEurycoma longifoliasupplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient osteoporosis model). Forty-eight-aged (10–12 months old)Sprague Dawleyrats were divided into six groups of sham-operated (SHAM), orchidectomised control (ORX), orchidectomised + 7 mg/rat testosterone enanthate (TEN) and orchidectomised +Eurycoma longifolia30 mg/kg (EL30), orchidectomised +Eurycoma longifolia60 mg/kg (EL60), orchidectomised +Eurycoma longifolia90 mg/kg (EL90). Rats were euthanized following six weeks of treatment. The left femora were used to measure the trabecular bone microarchitecture using micro-CT. Orchidectomy significantly decreased connectivity density, trabecular bone volume, and trabecular number compared to the SHAM group. Testosterone replacement reversed all the orchidectomy-induced changes in the micro-CT parameters. EL at 30 and 60 mg/kg rat worsened the trabecular bone connectivity density and trabecular separation parameters of orchidectomised rats. EL at 90 mg/kg rat preserved the bone volume. High dose of EL (90 mg/kg) may have potential in preserving the bone microarchitecture of orchidectomised rats, but lower doses may further worsen the osteoporotic changes.

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A Comparison of Micro-CT and Dental CT in Assessing Cortical Bone Morphology and Trabecular Bone Microarchitecture

PLoS ONE ◽

10.1371/journal.pone.0107545 ◽

2014 ◽

Vol 9 (9) ◽

pp. e107545 ◽

Cited By ~ 19

Author(s):

Jui-Ting Hsu ◽

Ying-Ju Chen ◽

Jung-Ting Ho ◽

Heng-Li Huang ◽

Shun-Ping Wang ◽

...

Keyword(s):

Trabecular Bone ◽

Cortical Bone ◽

Bone Microarchitecture ◽

Micro Ct ◽

Bone Morphology ◽

Trabecular Bone Microarchitecture ◽

Dental Ct

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Accentuated osseointegration in osteogenic nanofibrous coated titanium implants

Scientific Reports ◽

10.1038/s41598-019-53884-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Siddhartha Das ◽

Kanchan Dholam ◽

Sandeep Gurav ◽

Kiran Bendale ◽

Arvind Ingle ◽

...

Keyword(s):

Biomechanical Testing ◽

Titanium Implants ◽

Micro Ct ◽

Osseous Tissue ◽

Proof Of Concept ◽

Pathological Changes ◽

Histomorphometric Analysis ◽

Micro Computed Tomography ◽

Endosseous Implant ◽

Gross Examination

AbstractAnchoring of endosseous implant through osseointegration continues to be an important clinical need. Here, we describe the development of superior endosseous implant demonstrating enhance osseointegration, achieved through surface modification via coating of osteogenic nanofibres. The randomized bio-composite osteogenic nanofibres incorporating polycaprolactone, gelatin, hydroxyapatite, dexamethasone, beta-glycerophosphate and ascorbic acid were electrospun on titanium implants mimicking bone extracellular matrix and subsequently induced osteogenesis by targeting undifferentiated mesenchymal stem cells present in the peri-implant niche to regenerate osseous tissue. In proof-of-concept experiment on rabbit study models (n = 6), micro-computed tomography (Micro-CT), histomorphometric analysis and biomechanical testing in relation to our novel osteogenic nanofibrous coated implants showed improved results when compared to uncoated controls. Further, no pathological changes were detected during gross examination and necropsy on peri-implant osseous tissues regenerated in response to such coated implants. The findings of the present study confirm that osteogenic nanofibrous coating significantly increases the magnitude of osteogenesis in the peri-implant zone and favours the dynamics of osseointegration.

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Micro-CT–Based Bone Microarchitecture Analysis of the Murine Skull

Methods in Molecular Biology - Craniofacial Development ◽

10.1007/978-1-0716-1847-9_10 ◽

2021 ◽

pp. 129-145

Author(s):

Jenny Tan ◽

Agatha Labrinidis ◽

Ruth Williams ◽

Mustafa Mian ◽

Peter J. Anderson ◽

...

Keyword(s):

Bone Microarchitecture ◽

Micro Ct

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The effects of Labisia pumila extracts on bone microarchitecture of ovariectomized-induced osteoporosis rats: A micro-CT analysis

Journal of X-Ray Science and Technology ◽

10.3233/xst-16115 ◽

2017 ◽

Vol 25 (1) ◽

pp. 101-112

Author(s):

Nadia Mohd Effendy ◽

Mohd Fadhli Khamis ◽

Ahmad Nazrun Shuid

Keyword(s):

Bone Microarchitecture ◽

Micro Ct ◽

Ct Analysis ◽

Labisia Pumila

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Technetium-99 Conjugated with Methylene Diphosphonate Ameliorates Glucocorticoid Induced Osteoporosis by Inhibiting Osteoclastogenesis

BioMed Research International ◽

10.1155/2018/7902760 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9

Author(s):

Lianjie Shi ◽

Ying Ning ◽

Liling Xu ◽

Jianhong Li ◽

Xuewu Zhang

Keyword(s):

Cortical Bone ◽

Bone Microarchitecture ◽

Osteoclast Differentiation ◽

Bone Geometry ◽

Negative Control ◽

High Dose ◽

Micro Ct ◽

Sprague Dawley ◽

Blank Group ◽

Methylene Diphosphonate

Technetium-99 conjugated with methylene diphosphonate (99Tc-MDP) is an effective anti-inflammatory drug in treating rheumatoid arthritis (RA) for over 15 years in China. However, as a special form of bisphosphonate, the antiosteoporotic effect of99Tc-MDP is unclear. We systematically investigated the effects of99Tc-MDP on cancellous and cortical bone, respectively, in glucocorticoid induced osteoporosis (GIO) animal models. Forty-eight Sprague-Dawley rats were randomly divided into six groups: blank, negative control, high dose, medium dose, low dose, and positive control groups. After dexamethasone was given to all groups except the blank group to induce osteoporosis, the rats in different groups were treated with saline, MDP, or different doses of99Tc-MDP. After treatment, all rats were sacrificed, and their tibiae and femora were analyzed with microcomputed tomography (micro-CT), histology and biomechanics. Micro-CT analyses showed that (1)99Tc-MDP reversed glucocorticoid induced bone microarchitecture destruction by increasing BV/TV, Tb.Th, and Tb.N and decreasing BS/BV, Tb.Sp, and TBPf; (2) effect of99Tc-MDP increased as its dosage increased; and (3)99Tc-MDP could improve cortical bone thickness while MDP failed to do so. Micro-CT spatial structure analysis and histology also yielded consistent results, indicating that99Tc-MDP increased trabecular number and connectivity morphologically. Secondly, biomechanics revealed that99Tc-MDP can enhance the extrinsic stiffness of bone by changing bone geometry. Finally,99Tc-MDP could inhibit osteoclastogenesis in PBMCs in human. In conclusion,99Tc-MDP exerted antiosteoporotic effect by improving both cancellous and cortical bone, as well as increasing extrinsic bone stiffness which might be attributed to the its inhibition of osteoclast differentiation. The antiosteoporotic effect of99Tc-MDP may suggest a potential clinical application for patients with GIO.

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Contribution of Three-Dimensional Trabecular Bone Microstructure of the Proximal Femur to its Mechanical Properties as Assessed by Micro-Finite Element Analysis

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.321-323.278 ◽

2006 ◽

Vol 321-323 ◽

pp. 278-281

Author(s):

Wen Quan Cui ◽

Ye Yeon Won ◽

Myong Hyun Baek ◽

Kwang Kyun Kim

Keyword(s):

Finite Element Analysis ◽

Finite Element ◽

Trabecular Bone ◽

Young’S Modulus ◽

Young's Modulus ◽

Bone Microarchitecture ◽

Element Model ◽

Micro Ct ◽

Micro Computed Tomography ◽

Element Analysis

The purpose of this study was to investigate the contribution of the microstructural properties of trabecular bone in predicting its elastic modulus in the intertrochanteric region. A total of 15 trabecular bone core specimens were obtained from the proximal femurs of patients undergoing total hip arthroplasty. The micro-computed tomography (micro-CT) was used to scan each specimen to obtain micro-morphology. Microstructural parameters were directly calculated using software. Micro-CT images were converted to micro-finite element model using meshing technique, and then micro-finite element analysis (FEA) was performed to assess the mechanical property (Young’s modulus) of trabecular bone. The results showed that the ability to explain this variance of Young’s modulus is improved by combining the structural indices with each other. It suggested that assessment of bone microarchitecture should be added as regards detection of osteoporosis and evaluation of the efficacy of drug treatments for osteoporosis.

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