Micro-CT–Based Bone Microarchitecture Analysis of the Murine Skull

2021 ◽  
pp. 129-145
Author(s):  
Jenny Tan ◽  
Agatha Labrinidis ◽  
Ruth Williams ◽  
Mustafa Mian ◽  
Peter J. Anderson ◽  
...  
Keyword(s):  
Bone Microarchitecture ◽  
Micro Ct

scholarly journals Bone Micro-CT Assessments in an Orchidectomised Rat Model Supplemented withEurycoma longifolia

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Rosmaliza Ramli ◽  
Mohd Fadhli Khamis ◽  
Ahmad Nazrun Shuid
Keyword(s):  
Trabecular Bone ◽  
Bone Microarchitecture ◽  
Bone Volume ◽  
High Dose ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Elderly Male ◽  
Trabecular Bone Microarchitecture ◽  
Eurycoma Longifolia ◽  
Induced Changes

Recent studies suggested thatEurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects ofEurycoma longifoliasupplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient osteoporosis model). Forty-eight-aged (10–12 months old)Sprague Dawleyrats were divided into six groups of sham-operated (SHAM), orchidectomised control (ORX), orchidectomised + 7 mg/rat testosterone enanthate (TEN) and orchidectomised +Eurycoma longifolia30 mg/kg (EL30), orchidectomised +Eurycoma longifolia60 mg/kg (EL60), orchidectomised +Eurycoma longifolia90 mg/kg (EL90). Rats were euthanized following six weeks of treatment. The left femora were used to measure the trabecular bone microarchitecture using micro-CT. Orchidectomy significantly decreased connectivity density, trabecular bone volume, and trabecular number compared to the SHAM group. Testosterone replacement reversed all the orchidectomy-induced changes in the micro-CT parameters. EL at 30 and 60 mg/kg rat worsened the trabecular bone connectivity density and trabecular separation parameters of orchidectomised rats. EL at 90 mg/kg rat preserved the bone volume. High dose of EL (90 mg/kg) may have potential in preserving the bone microarchitecture of orchidectomised rats, but lower doses may further worsen the osteoporotic changes.

scholarly journals A Comparison of Micro-CT and Dental CT in Assessing Cortical Bone Morphology and Trabecular Bone Microarchitecture

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107545 ◽  
Author(s):  
Jui-Ting Hsu ◽  
Ying-Ju Chen ◽  
Jung-Ting Ho ◽  
Heng-Li Huang ◽  
Shun-Ping Wang ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Cortical Bone ◽  
Bone Microarchitecture ◽  
Micro Ct ◽  
Bone Morphology ◽  
Trabecular Bone Microarchitecture ◽  
Dental Ct

Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis

2018 ◽  
Vol 76 (5) ◽  
pp. 972-981
Author(s):  
Carolina Cintra Gomes ◽  
Deborah Queiroz Freitas ◽  
Amanda Maria Medeiros Araújo ◽  
Laura Ricardina Ramírez-Sotelo ◽  
Fernanda Paula Yamamoto-Silva ◽  
...  
Keyword(s):  
Bone Microarchitecture ◽  
Micro Ct ◽  
Histomorphometric Analysis

scholarly journals Technetium-99 Conjugated with Methylene Diphosphonate Ameliorates Glucocorticoid Induced Osteoporosis by Inhibiting Osteoclastogenesis

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Lianjie Shi ◽  
Ying Ning ◽  
Liling Xu ◽  
Jianhong Li ◽  
Xuewu Zhang
Keyword(s):  
Cortical Bone ◽  
Bone Microarchitecture ◽  
Osteoclast Differentiation ◽  
Bone Geometry ◽  
Negative Control ◽  
High Dose ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Blank Group ◽  
Methylene Diphosphonate

Technetium-99 conjugated with methylene diphosphonate (99Tc-MDP) is an effective anti-inflammatory drug in treating rheumatoid arthritis (RA) for over 15 years in China. However, as a special form of bisphosphonate, the antiosteoporotic effect of99Tc-MDP is unclear. We systematically investigated the effects of99Tc-MDP on cancellous and cortical bone, respectively, in glucocorticoid induced osteoporosis (GIO) animal models. Forty-eight Sprague-Dawley rats were randomly divided into six groups: blank, negative control, high dose, medium dose, low dose, and positive control groups. After dexamethasone was given to all groups except the blank group to induce osteoporosis, the rats in different groups were treated with saline, MDP, or different doses of99Tc-MDP. After treatment, all rats were sacrificed, and their tibiae and femora were analyzed with microcomputed tomography (micro-CT), histology and biomechanics. Micro-CT analyses showed that (1)99Tc-MDP reversed glucocorticoid induced bone microarchitecture destruction by increasing BV/TV, Tb.Th, and Tb.N and decreasing BS/BV, Tb.Sp, and TBPf; (2) effect of99Tc-MDP increased as its dosage increased; and (3)99Tc-MDP could improve cortical bone thickness while MDP failed to do so. Micro-CT spatial structure analysis and histology also yielded consistent results, indicating that99Tc-MDP increased trabecular number and connectivity morphologically. Secondly, biomechanics revealed that99Tc-MDP can enhance the extrinsic stiffness of bone by changing bone geometry. Finally,99Tc-MDP could inhibit osteoclastogenesis in PBMCs in human. In conclusion,99Tc-MDP exerted antiosteoporotic effect by improving both cancellous and cortical bone, as well as increasing extrinsic bone stiffness which might be attributed to the its inhibition of osteoclast differentiation. The antiosteoporotic effect of99Tc-MDP may suggest a potential clinical application for patients with GIO.

Contribution of Three-Dimensional Trabecular Bone Microstructure of the Proximal Femur to its Mechanical Properties as Assessed by Micro-Finite Element Analysis

2006 ◽  
Vol 321-323 ◽  
pp. 278-281
Author(s):  
Wen Quan Cui ◽  
Ye Yeon Won ◽  
Myong Hyun Baek ◽  
Kwang Kyun Kim
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Trabecular Bone ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Bone Microarchitecture ◽  
Element Model ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Element Analysis

The purpose of this study was to investigate the contribution of the microstructural properties of trabecular bone in predicting its elastic modulus in the intertrochanteric region. A total of 15 trabecular bone core specimens were obtained from the proximal femurs of patients undergoing total hip arthroplasty. The micro-computed tomography (micro-CT) was used to scan each specimen to obtain micro-morphology. Microstructural parameters were directly calculated using software. Micro-CT images were converted to micro-finite element model using meshing technique, and then micro-finite element analysis (FEA) was performed to assess the mechanical property (Young’s modulus) of trabecular bone. The results showed that the ability to explain this variance of Young’s modulus is improved by combining the structural indices with each other. It suggested that assessment of bone microarchitecture should be added as regards detection of osteoporosis and evaluation of the efficacy of drug treatments for osteoporosis.

Type I Diabetes Mellitus and Insulin Therapy on Bone Microarchitecture, Composition and Mechanical Properties

2021 ◽  
Vol 18 ◽  
Author(s):  
Pedro Henrique Justino Oliveira Limirio ◽  
Priscilla Barbosa Ferreira Soares ◽  
Jessyca Figueira Venâncio ◽  
Gustavo Davi Rabelo ◽  
Carlos José Soares ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Mechanical Properties ◽  
Cortical Thickness ◽  
Type I Diabetes ◽  
Bone Microarchitecture ◽  
Crystallinity Index ◽  
Diabetic Group ◽  
Type I ◽  
Micro Ct ◽  
Insulin Group

Background: The aim of this study was to evaluate the microarchitecture, composition and mechanical properties of cortical bone of rats with type I diabetes mellitus (TIDM) and submitted to insulin therapy (IT). Methods: Thirty rats were divided into three groups (n=10): non-diabetic, diabetic and diabetic+insulin. TIDM was induced by intravenous injection of streptozotocin. In diabetic+insulin group, 4IU insulin was administered twice per day (1IU at 7am and 3IU at 7pm). The animals were euthanized five weeks after TIDM induction; the tibiae were removed and submitted to microcomputed tomography (micro-CT, 8µm), fourier transform infrared spectroscopy (FTIR) and dynamic microhardness indentation. Results: Micro-CT analysis showed that diabetic group had lower bone surface/tissue volume ratio (BS/BV) (p=0.018), cortical thickness (Ct.Th) (p<0.001) and degree of anisotropy (Ct.DA) (p=0.034) values compared to non-diabetic group. The diabetic group showed lower Ct.Th than diabetic + insulin group (p=0.018). The non-diabetic group had lower fractal dimension (Ct.FD) values compared to diabetic groups (p<0.001). The ATR-FTIR analyses showed lower values for all measured parameters in the diabetic group than non-diabetic group (amide I ratio: p=0.046; crystallinity index: p=0.038; matrix:mineral ratios - M:MI: p=0.006; M:MIII: p=0.028). The diabetic+insulin group showed a lower crystallinity index (p=0.022) and M:MI ratio (p=0.002) than non-diabetic and diabetic groups, respectively. The diabetic group showed lower Vickers hardness values than non-diabetic (p<0.001) and diabetic+insulin (p=0.003) groups. Conclusion: TIDM negatively affect bone microarchitecture, collagen maturation, mineralization and bone microhardness. Moreover, insulin minimized the effect of TIDM on cortical thickness and organic/mineral matrix.

Deterioration of Cortical Bone Microarchitecture: Critical Component of Renal Osteodystrophy Evaluation

2018 ◽  
Vol 47 (6) ◽  
pp. 376-384 ◽  
Author(s):  
Ashish K. Sharma ◽  
Nigel D. Toussaint ◽  
Rosemary Masterson ◽  
Stephen G. Holt ◽  
Chamith S. Rajapakse ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Cortical Bone ◽  
Renal Osteodystrophy ◽  
Bone Microarchitecture ◽  
Structural Parameters ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Micro Ct ◽  
Normal Ranges ◽  
Bone Biopsies

Background: Cortical bone is a significant determinant of bone strength and its deterioration contributes to bone fragility. Thin cortices and increased cortical porosity have been noted in patients with chronic kidney disease (CKD), but the “Turnover Mineralization Volume” classification of renal osteodystrophy does not emphasize cortical bone as a key parameter. We aimed to assess trabecular and cortical bone microarchitecture by histomorphometry and micro-CT in patients with CKD G5 and 5D (dialysis). Methods: Transiliac bone biopsies were performed in 14 patients undergoing kidney transplantation (n = 12) and parathyroidectomy (n = 2). Structural parameters were analysed by histomorphometry and micro-CT including trabecular bone volume, thickness (TbTh), number (TbN) and separation and cortical thickness (CtTh) and porosity (CtPo). Indices of bone remodelling and mineralisation were obtained and relationships to bone biomarkers examined. Associations were determined by Spearman’s or Pearson’s rank correlation coefficients. Results: By micro-CT, trabecular parameters were within normal ranges in most patients, but all patients showed very low CtTh (127 ± 44 µm) and high CtPo (60.3 ± 22.5%). CtPo was inversely related to TbN (r = –0.56; p = 0.03) by micro-CT and to TbTh (r = –0.60; p = 0.024) by histomorphometry and correlated to parathyroid hormone values (r = 0.62; p = 0.021). By histomorphometry, bone turnover was high in 50%, low in 21% and normal in 29%, while 36% showed abnormal patterns of mineralization. Significant positive associations were observed between osteoblast surface, osteoclast surface, mineralization surface and bone turnover markers. Conclusions: Deterioration of cortical ­microarchitecture despite predominantly normal trabecular parameters reinforces the importance of comprehensive cortical evaluation in patients with CKD.

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