Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species

Increased concentrations of partially reduced oxygen and nitrogen reactive species damage the alveolar epithelium and either cause or exacerbate surfactant deficiency. For this reason, there is a quest to identify surfactant replacement mixtures, which in addition to repleting depleted surfactant stores can also reduce the steady-state concentrations of reactive species in the alveolar space. Herein, we evaluated the ability of natural lung surfactant (NLS) and two mixtures (Exosurf and Survanta) used clinically for the correction of surfactant deficiency to scavenge hydroxyl radical-type species (.OH), generated either by the decomposition of peroxynitrite or by Fenton reagents (FeCl3 + H2O2). Exosurf or Survanta decreased .OH only when present at high lipid concentrations (6.5 mM). On the other hand, 40 microM of NLS decreased .OH concentrations from 75 +/- 2 to 53 +/- 2 microM (P < 0.05), most likely because of the interaction of .OH with protein sulfhydryl groups. Similarly, 40 microM of NLS incubated with a bolus of H2O2 (400 microM) decreased the H2O2 concentration in the supernatant by approximately 50%, due to the presence of catalase-type activity. In contrast to NLS, neither Exosurf nor Survanta scavenged H2O2, even when present at millimolar lipid concentrations. We concluded that Exosurf and Survanta contain limited antioxidant activity compared with NLS.

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Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/333/1/012052 ◽

2019 ◽

Vol 333 ◽

pp. 012052

Author(s):

S Cvetkovic ◽

S Djukanovic ◽

D Mitic-Culafic ◽

B Nastasijevic ◽

J Knezevic-Vukcevic ◽

...

Keyword(s):

Protective Effect ◽

Aromatic Amines ◽

Heterocyclic Aromatic Amines ◽

Processed Meat ◽

Leaf Extracts ◽

Gentiana Lutea ◽

Thermally Processed

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PROTECTIVE EFFECT OF SALIX TETRASPERMA ROXBURGH LEAF EXTRACTS AGAINST CARBON TETRACHLORIDE INDUCED LIVER INJURY IN RATS

International Research Journal of Pharmacy ◽

10.7897/2230-8407.0811222 ◽

2017 ◽

Vol 8 (11) ◽

pp. 81-84

Author(s):

J H Virupaksha ◽

Ramarao Nadendla ◽

A Narendra Babu ◽

M Komali

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Protective Effect ◽

Leaf Extracts

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Ti4O7 Used as Electrode in Biomedicine and for Electrochemical Study of Scavenging Mechanism

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.896 ◽

2011 ◽

Vol 493-494 ◽

pp. 896-901

Author(s):

María Canillas ◽

Ann Rajnicek ◽

C. Rosero ◽

Eva Chinarro ◽

Berta Moreno

Keyword(s):

Oxidation State ◽

Reactive Species ◽

Electrochemical Methods ◽

Electrochemical Study ◽

Scavenging Activity ◽

Reduction Reactions ◽

Oxidation Reduction ◽

Nitrogen Reactive Species

The biocompatibility of TiO2 is due to the activity that it shown in front of oxygen and nitrogen reactive species. Some authors suggest that the mechanism go through oxidation reduction reactions where changes of oxidation state in the Titanium and phases are involve. For this reason, Anderson-Magnelli phases could present scavenging activity. Moreover, these materials are use as electrodes and in that way are proposed as electrodes for study their scavenging mechanism by electrochemical methods.

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Total Phenolics, Flavonoids Contents, Antioxidant Activity and DNA Protective Effect of Lenten Rose (Helleborus orientalis)

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2018/v1i430047 ◽

2019 ◽

pp. 1-12

Author(s):

Meral Öztürk ◽

İbrahim Bulduk ◽

Safiye Elif Korcan ◽

Recep Liman ◽

Funda Karabağ Çoban ◽

...

Keyword(s):

Antioxidant Activity ◽

Protective Effect ◽

Leaf Extract ◽

Total Phenol Content ◽

Total Flavonoid Content ◽

Phenol Content ◽

Flavonoid Content ◽

Leaf Extracts ◽

Helleborus Orientalis ◽

Different Parts

The aims of the present study were to evaluate the antioxidant activity and DNA Protective effect of Helleborus orientalis (HO) leaf extract against oxidative damage, and to determine the total phenolic and flavonoid contents of the plant species studied. Methods: The total phenol content (TPC) of H. orientalis (Ranunculaceae) extract was determined using the Folin-Ciocalteu technique. The aluminum chloride colorimetric assay in the determination of The total flavonoid content (TFC) and was used, Analysis of Phenolic Acids was identified by High-Performance Liquid Chromatography (HPLC). Antioxidant activity was analyzed by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Assay. Protective effect of H. orientalis leaf extract against to H2O2 was evaluated by using TAS, TOS methods and Comet assay. Key Findings: H. orientalis ethanol extracts contain high amounts of antioxidants. The HO leaf methanol extract (LME) decreased the DNA damage at all tested concentrations in a dose-dependent manner (r=0.86 p<0.01) against to H2O2. Conclusions: The total phenol content in the extracts of different parts of the plant varied from 4.00 mg GAE/1 gr to 19.42 mg GAE/1 gr. The flowers had the highest phenol content (19.42 mg GAE/1 gr sample) and followed by the leaves (17.20 mg GAE/1 gr sample). The total flavonoid content in the extracts from different parts of the plant varied from 2.57 mg QE/1 gr to 11.88 mg QE/1 gr. The flowers had the highest flavonoid content (11.88 mg QE/1 gr sample) and followed by the leaves (10.21 mg QE/1 gr sample). Antioxidant activity of fractions was explained as a percentage of DPPH radicals’ scavenging and IC50 values (μg/ml). Leaf and flowers of HO are richer in antioxidant than its root and stem. As the concentration of leaf extracts used increased, the DNA protective effect increased and it was statistically significant at overdoses of 2500 μg/mL. Total antioxidant status (TAS) levels were significantly (p <0.05) decreased in the H2O2 group (3.4±0.21) but H. orientalis was significantly (p<0.05) increased TAS levels in this group. When the concentration of leaf extracts used increased, the protective effect has also increased and statistically significant at overdoses of 2500 μg / mL (6.3±0.67). Total oxidant status (TOS) levels were significantly (p <0.05) increased in the H2O2 group (25.3±0.74) and H. orientalis was significantly (p<0.05) decreased TOS levels in groups.

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PROTECTIVE EFFECT OF CASSIA FISTULA EXTRACTS AGAINST ULTRAVIOLET C INJURY IN HUMAN CORNEAL EPITHELIAL CELLS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i2.29562 ◽

2019 ◽

pp. 261-266

Author(s):

DEEKSHA K ◽

CYNTHIA ARUNACHALAM

Keyword(s):

Antioxidant Activity ◽

Protective Effect ◽

Cell Viability ◽

Radical Scavenging ◽

Reducing Power ◽

Cassia Fistula ◽

Leaf Extracts ◽

Corneal Epithelial ◽

Ultraviolet C ◽

Uvc Radiation

Objective: The objective of this study was to investigate the antioxidant activity of Indian laburnum (Cassia fistula L. [CF]) leaf extracts and their impact on ultraviolet C (UVC) radiation-induced damage on human corneal epithelial (HCE) cells. Methods: The antioxidant activity and free radical scavenging ability of CF leaf extracts were determined by in vitro methods such as 1,1-diphenyl- 2-picrylhydrazyl radical scavenging capacity, Total antioxidant capacity (TAC), and reducing power. The total phenolic content (TPC), total flavonoid content (TFC), and preliminary phytochemical screening were done to ensure the pharmacological effects of the extracts. The UVC radiation at wavelength 254 nm was used to irradiate HCE cells and cell viability was assessed by methyl thiazolyl tetrazolium assay. Results: Extracts at the concentration of 200 μg/ml did not affect the cell viability of HCE cells. Almost 50% cell death was observed after UVC irradiation at a dose of 200 J/m2. Both extracts showed a protective effect by increasing the cell viability of irradiated cells up to 57.28% and 62.39%. A dose-dependent increase in the TAC and reducing power of the extract was observed. The TPC in aqueous and ethanol extracts of CF leaves was 18.8 and 27.80 mg gallic acid equivalent/g sample while TFC was 8.47 and 16.5 mg quercetin equivalent per/gsample, respectively. Conclusions: CF leaves are a potent source of bioactive compounds with good antioxidant potential. Exposure to UVC radiation cause harmful effects on HCE cells and the extracts have shown to have potent protective effects on UV light-induced oxidative stress in HCE cells.

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