Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets

2019 ◽  
Vol 220 ◽  
pp. 137-142 ◽  
Author(s):  
X. Zong ◽  
T.H. Wang ◽  
Z.Q. Lu ◽  
D.G. Song ◽  
J. Zhao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bacillus Licheniformis ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Clostridium Butyricum ◽  
Intestinal Barrier Function ◽  
Weanling Piglets

scholarly journals Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 527
Author(s):  
Jie Fu ◽  
Tenghao Wang ◽  
Xiao Xiao ◽  
Yuanzhi Cheng ◽  
Fengqin Wang ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacillus Licheniformis ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Clostridium Butyricum ◽  
Intestinal Barrier Function ◽  
Weaned Piglets ◽  
Total Acid ◽  
Junction Protein ◽  
Mucin Expression

This study investigated the effects of dietary C. butyricum ZJU-F1 on the apparent digestibility of nutrients, intestinal barrier function, immune response, and microflora of weaned piglets, with the aim of providing a theoretical basis for the application of Clostridium butyricum as an alternative to antibiotics in weaned piglets. A total of 120 weanling piglets were randomly divided into four treatment groups, in which piglets were fed a basal diet supplemented with antibiotics (CON), Bacillus licheniformis (BL), Clostridium butyricum ZJU-F1 (CB), or Clostridium butyricum and Bacillus licheniformis (CB-BL), respectively. The results showed that CB and CB-BL treatment increased the intestinal digestibility of nutrients, decreased intestinal permeability, and increased intestinal tight junction protein and mucin expression, thus maintaining the integrity of the intestinal epithelial barrier. CB and CB-BL, as exogenous probiotics, were also found to stimulate the immune response of weaned piglets and improve the expression of antimicrobial peptides in the ileum. In addition, dietary CB and CB-BL increased the proportion of Lactobacillus. The levels of butyric acid, propionic acid, acetic acid, and total acid were significantly increased in the ceca of piglets fed CB and CB-BL. Furthermore, we validated the effects of C. butyricum ZJU-F1 on the intestinal barrier function and immune response in vitro and found C. butyricum ZJU-F1 improved intestinal function and enhanced the TLR-2-MyD88-NF-κB signaling.

scholarly journals Effects of dietary Clostridium butyricum supplementation on growth performance, intestinal barrier function, immune response, and intestinal permeability in broiler chickens with necrotic enteritis

2020 ◽  
Author(s):  
xiao xu ◽  
Shunli Yang ◽  
Joshua Seun Olajide ◽  
Zigang Qu ◽  
Zhenxing Gong ◽  
...  
Keyword(s):  
Growth Performance ◽  
Barrier Function ◽  
Average Daily Gain ◽  
Intestinal Barrier ◽  
Clostridium Butyricum ◽  
Intestinal Morphology ◽  
Intestinal Barrier Function ◽  
Intestinal Damage ◽  
Necrotic Enteritis ◽  
Intestinal Pathogens

Abstract Background Necrotic enteritis (NE), which is caused by Clostridium perfringens (C. perfringens), is an economically important disease in broiler. Among normal flora in the broiler intestinal, Clostridium butyricum (C. butyricum) has been identified as a probiotic agent that reduces the susceptibility of broilers to C. perfringens. It also promotes the repair of broiler intestinal damage from harmful intestinal pathogens. However, the effects of C. butyricum infection on broiler intestinal integrity during NE are largely unknown. In this study, we investigated the effects of C. butyricum on the growth performance, intestinal morphology and barrier function, and the functions of immune-related cytokines under NE in broilers. Results Infected group (PC) showed significant decrease in the average daily gain (ADG, p < 0.01) and increase in feed conversion ratio (FCR, p < 0.01) compared with C. butyricum (NECB1 and NECB2) through dietary supplement. Supplementation of C. butyricum (NECB1 and NECB2) restored the intestinal villus height, increased the crypt depth, and reduced the intestinal damage under NE. Furthermore, broilers infected with NE (PC) had higher serum IgA and endotoxin content, and after addition with C. butyricum (NECB1 and NECB2) returned to normal level. In addition, compared with PC, supplementation of C. butyricum (NECB1 and NECB2) restored intestinal barrier function-related genes (such as CLDN-1, CLDN-3, OCLN, MUC2, ZO-1, and CLDN5), cytokines (such as TNF-α, IL-10, IL-6, and TGFB1) and C. perfringens plc gene expression. Moreover, C. butyricum (NECB1 and NECB2) could restore the decrease in Gt (p < 0.01) and Isc caused by NE (PC). In addition, NECB2 reduced the upregulation of FD4 flux caused by NE infection (p < 0.01). Conclusion Dietary supplementation of C. butyricum into broilers with NE improved chicken growth performance, intestinal morphology, intestinal barrier function, and immune function. Notably, no statistical difference was observed with the addition of C. butyricum at different time points.

scholarly journals Effects of Clostridium butyricum on Growth Performance, Gut Microbiota and Intestinal Barrier Function of Broilers

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenjia Li ◽  
Bin Xu ◽  
Linyi Wang ◽  
Quanyou Sun ◽  
Wen Deng ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Barrier Function ◽  
Stocking Density ◽  
Intestinal Barrier ◽  
Basal Diet ◽  
The Body ◽  
Clostridium Butyricum ◽  
Intestinal Barrier Function ◽  
Significant Difference

This study was conducted to evaluate the effects of Clostridium butyricum dietary supplementation on the growth, antioxidant, immune response, gut microbiota, and intestinal barrier function of broilers under high stocking density (HSD) stress. A total of 324 1-day-old Arbor Acres male broilers were randomly assigned to three treatments with six replicates, each replicate including 18 chickens (18 birds/m2). The experiment lasted 6 weeks. The three treatments were basal diet (control, CON), basal diet supplemented with 1 × 109 colony forming units (cfu)/kg C. butyricum (CB), and basal diet supplemented with 10 mg/kg virginiamycin (antibiotic, ANT). The results showed that the body weight (BW) and average daily gain (ADG) of broilers in the CB group were significantly higher than those in the CON group in three periods (p &lt; 0.05). The total antioxidant capacity (T-AOC) and the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity in serum of the CB group were significantly increased compared with those in the CON and ANT groups at 42 days (p &lt; 0.05). At 42 days, the serum immunoglobulin M (IgM) and immunoglobulin G (IgG) levels of the CB group were significantly higher than those of the CON group. Compared with the CON group, interleukin-1β (IL-1β) in the CB group was significantly decreased in the starter and grower stages (p &lt; 0.05), but there was no significant difference between the two treatment groups (p &gt; 0.05). C. butyricum significantly decreased the high stocking density-induced expression levels of IL-1β and tumor necrosis factor-α (TNF-α) in the ileum of broilers at different stages. Additionally, C. butyricum could increase the expressions of claudin-1 and zonula occludens-1 (ZO-1) in intestinal tissue. Moreover, C. butyricum significantly increased the Sobs and Shannon indices in the CB group compared with the ANT group (p &lt; 0.05), while the Ace index in the CB group was significantly higher than that of the CON group (p &lt; 0.05). Furthermore, by using 16S rRNA gene sequencing, the proportion of Bacteroides in the CB group was increased compared to those in the CON and ANT groups at the genus level. In conclusion, C. butyricum supplemented into feed could improve the growth performance and feed utilization of broilers by promoting immune and intestinal barrier function and benefiting the cecal microflora.

scholarly journals Effects of Guava (Psidium Guajava L.) Leaf Extract on Growth Performance and Metabolomics in Weaned Piglets Challenged by Escherichia Coli

2021 ◽  
Author(s):  
Dingfa Wang ◽  
Luli Zhou ◽  
Hanlin Zhou ◽  
Guanyu Hou
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Dietary Supplementation ◽  
Intestinal Barrier Function ◽  
Control Group ◽  
Antioxidant Ability ◽  
Weaned Piglets ◽  
Diarrhea Incidence

Abstract Background: The effects of dietary supplementation with guava leaf extracts (GE) on growth performance, diarrhea and intestinal barrier function, as well as associated with its modulation of serum and fecal metabolic changes in weaned piglets challenged by enterotoxigenic Escherichia coli (ETEC) were investigated.Method: Fifty weaned piglets (Duroc × Yorkshire × Landrace) from 5 pens (2 piglets per pen) were randomly divided into five groups: blank control group (BC), negative control group (NC), or those supplemented with 50 mg kg-1 (S50), 100 mg kg-1 (S100), or 200 (S200) mg kg-1 diet GE, respectively. On day 4, all piglets (except for BC) were orally challenged with about 1.0 × 109 colony-forming units (CFU) enterotoxigenic ETEC. After 28-day trial, growth performance, diarrhea incidence, intestinal barrier function and metabolomics of serum and fecal were investigated.Results: We demonstrated that dietary supplementation with GE (50-200 mg kg-1) reduced diarrhea incidence of piglets and increased expression of intestinal tight junction proteins (ZO-1, Occludin, Claudin-1) (P < 0.05) and sodium hydrogen exchanger 3 (NHE3) (P < 0.05). Moreover, dietary supplementation with GE (50-200 mg kg-1) upregulated level of tetrahydrofolic acid (THF) and reversed higher level of nicotinamide-adenine dinucleotide phosphate (NADP) caused by ETEC in serum compared with NC group (P < 0.05), and enhanced antioxidant ability of piglets. In addition, dietary addition with GE (100 mg kg-1) reversed the lower level of L-pipecolic acid caused by ETEC in feces compared with NC group (P < 0.05), and decreased oxidative stress response of piglets. Further, there were no differences (P > 0.05) in the final weight, average daily feed intake (ADFI) and F/G among dietary groups during the overall period, and piglets in S50 group has the higher average daily gain (ADG). Conclusion: Dietary supplementation with 50-200 mg kg-1 GE reduced diarrhea incidence of weaned piglets challenged by ETEC and exhibited positive effect on improving intestinal barrier function. Meanwhile, dietary addition with GE organized and redistributed energy resources through similar or dissimilar metabolic pathways, and finally enhanced antioxidant ability of piglets challenged by ETEC.

scholarly journals Effects of Fermented Feed on the Growth Performance, Intestinal Function and Microbiota of Piglets Weaned at Different Age

2021 ◽  
Author(s):  
Shuai Liu ◽  
Yunxia Xiong ◽  
Jingping Chen ◽  
Hao Xiao ◽  
Qiwen Wu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Barrier Function ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Dietary Supplementation ◽  
Short Chain Fatty Acids ◽  
Intestinal Barrier Function ◽  
Intestinal Function ◽  
Feed Conversion ◽  
Fermented Feed

Abstract BACKGROUND: The beneficial function of fermented feed in livestock industry has been widely investigated. However, little is known about the effects of fermented feed on different weaned-day piglets. This study aimed to investigate the effects of fermented diet on the growth performance, intestinal function and microbiota of piglets weaned at age of 21 days and 28 days.RESULTS: The results found that weaning on d 21 significantly increased (P < 0.05) ADG, and ADFI (calculated based on wet weight and dry matter), while reduced (P < 0.05) F: G, the activities of trypsin and lipase of jejunum and villus height of ileum, compared with 28-d weaning. The protein levels of Occludin, Claudin-1, ZO-1 of ileum in the groups weaning on d 21 were less (P < 0.05) than the groups weaning on d 28. Moreover, dietary supplementation with fermented diet upregulated (P < 0.05) Occludin, Claudin-1, ZO-1 proteins of ileum, compared with the groups treated with control diet both weaning on d 21 and d 28. In addition, dietary supplementation with fermented diet decreased (P < 0.05) the relative abundance of Clostridia (class) and increased (P < 0.05) Bacteroidia (class) level of cecal microbiota, compared with the groups treated with control diet both weaning on d 21 and d 28. However, supplementation with fermented diet did not affect the concentrations of short-chain fatty acids in the cecum (P > 0.05).CONCLUSION: Therefore, our data suggest that feed digestibility is improved in piglets weaned at 21 days, but intestinal barrier function is weaker than in piglets weaned at 28 days. However, compared with feeding control diet, supplementation with fermented diet both improved feed conversion and intestinal barrier function of weaned piglets by modulating intestinal microbiota.

The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs

2015 ◽  
Vol 95 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Bich Van Le Thanh ◽  
Martin Lessard ◽  
Younès Chorfi ◽  
Frédéric Guay
Keyword(s):  
Adverse Effects ◽  
Growth Performance ◽  
Barrier Function ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Nutrient Digestibility ◽  
Feed Additives ◽  
Intestinal Barrier Function ◽  
Fusarium Mycotoxins ◽  
Weanling Pigs

Thanh, B. V. L., Lessard, M., Chorfi, Y. and Guay, F. 2015. The efficacy of anti-mycotoxin feed additives in preventing the adverse effects of wheat naturally contaminated with Fusarium mycotoxins on performance, intestinal barrier function and nutrient digestibility and retention in weanling pigs. Can. J. Anim. Sci. 95: 197–209. This study was designed to determine the effect of feeding deoxynivalenol (DON) contaminated wheat to growing pigs on growth performance, digestibility and retention of nitrogen (N), phosphorus (P) and calcium (Ca). Secondly, we wished to evaluate the potential of four commercial anti-mycotoxin additives in preventing the adverse effects of DON. Sixty piglets (6.0 kg body weight) were assigned to six different corn-soybean meal-wheat diets (control diet: <0.5 mg kg−1 DON; contaminated diet (DON-contaminated): 4 mg kg−1; and four contaminated diets supplemented with a different anti-mycotoxin additive: DON+GLUC (glucomannan), DON+YBP (yeast, live bacteria, enzymes, plant extracts), DON+ALU (aluminosilicate) and DON+PC (preservation components). Piglets were housed individually in pens for 7 d and then transferred to metabolic crates for urine and feces collection for 7 d. At the end of these 2 wk, mannitol and lactulose doses were given by oral administration. Urine was collected for 24 h for an evaluation of barrier integrity of intestinal mucosa. The piglets were then euthanatized and intestinal samples collected for morphology studies. Feeding DON-contaminated wheat reduced average daily feed intake, average daily gain and G:F ratio compared with the control diet (P<0.05). Only DON+PC diet restored the growth performance of piglets fed DON-contaminated diet. Daily retention of N and P was not affected by DON contamination or anti-mycotoxin additives, but retention of Ca was higher in piglets fed the DON-contaminated diets than the control diet. DON-contaminated diet reduced digestibility of dry matter, gross energy and fat, villi height in jejunum, and recovery of mannitol and lactulose compared with the control diet (P<0.05). Finally, DON concentrations in serum from piglets fed the DON-contaminated diets were higher than in the control diet (P<0.05). Piglets fed DON-contaminated wheat had reduced growth possibly caused by impaired jejunal morphology and decreased digestibility of energy and fat.

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