Nutrient release and oxidative stability during in vitro digestion of linseed oil emulsions produced from cow milk, soy drink, and green tea extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro

Antiviral Research ◽

10.1016/j.antiviral.2007.11.011 ◽

2008 ◽

Vol 78 (3) ◽

pp. 242-249 ◽

Cited By ~ 54

Author(s):

Jun Xu ◽

Jue Wang ◽

Fei Deng ◽

Zhihong Hu ◽

Hualin Wang

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Green Tea Extract ◽

B Virus ◽

Tea Extract

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Ingredient-Dependent Extent of Lipid Oxidation in Margarine

Antioxidants ◽

10.3390/antiox10010105 ◽

2021 ◽

Vol 10 (1) ◽

pp. 105

Author(s):

Sarah Fruehwirth ◽

Sandra Egger ◽

Dennis Kurzbach ◽

Jakob Windisch ◽

Franz Jirsa ◽

...

Keyword(s):

Citric Acid ◽

Lipid Oxidation ◽

Oxidative Stability ◽

Green Tea ◽

Acyl Chain ◽

Green Tea Extract ◽

Tocopheryl Acetate ◽

Tea Extract ◽

The Impact ◽

Β Carotene

This study reports the impact of margarine-representative ingredients on its oxidative stability and green tea extract as a promising antioxidant in margarine. Oil-in-water emulsions received much attention regarding factors that influence their oxidative stability, however, water-in-oil emulsions have only been scarcely investigated. Margarine, a widely consumed water-in-oil emulsion, consists of 80–90% fat and is thermally treated when used for baking. As different types of margarine contain varying additives, their impact on the oxidative stability of margarine during processing is of pressing importance. Thus, the influence of different ingredients, such as emulsifiers, antioxidants, citric acid, β-carotene and NaCl on the oxidative stability of margarine, heated at 80 °C for 1 h to accelerate lipid oxidation, was analyzed by the peroxide value and oxidation induction time. We found that monoglycerides influenced lipid oxidation depending on their fatty acyl chain. α-Tocopheryl acetate promoted lipid oxidation, while rosemary and green tea extract led to the opposite. Whereas green tea extract alone showed the most prominent antioxidant effect, combinations of green tea extract with citric acid, β-carotene or NaCl increased lipid oxidation in margarine. Complementary, NMR data suggested that polyphenols in green tea extracts might decrease lipid mobility at the surface of the water droplets, which might lead to chelating of transition metals at the interface and decreasing lipid oxidation.

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Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In Vitro

Archives of Industrial Hygiene and Toxicology ◽

10.2478/10004-1254-62-2011-2105 ◽

2011 ◽

Vol 62 (2) ◽

pp. 139-146 ◽

Cited By ~ 6

Author(s):

Ksenija Durgo ◽

Sandra Kostić ◽

Katarina Gradiški ◽

Draženka Komes ◽

Maja Osmak ◽

...

Keyword(s):

Cell Line ◽

Green Tea ◽

Laryngeal Carcinoma ◽

Resistant Cell ◽

Resistant Cell Line ◽

Green Tea Extract ◽

Carcinoma Cells ◽

Tea Extract ◽

Human Laryngeal Carcinoma

Genotoxic Effects of Green Tea Extract on Human Laryngeal Carcinoma Cells In VitroGreen tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and prooxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its cross-resistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.

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Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502013000200016 ◽

2013 ◽

Vol 49 (2) ◽

pp. 341-349 ◽

Cited By ~ 7

Author(s):

Tariq Mahmood ◽

Naveed Akhtar ◽

Barkat Ali Khan ◽

Akhtar Rasul ◽

Haji M. Shoaib Khan

Keyword(s):

Green Tea ◽

Storage Conditions ◽

Green Tea Extract ◽

Viscosity Data ◽

Skin Protection ◽

Multiple Emulsion ◽

Multiple Emulsions ◽

Tea Extract

Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active (MeA) and without active (MeB). Both formulations showed comparable characteristics regarding various physicochemical characteristics in different storage conditions. Rheological analysis showed that formulations showed pseudo plastic behavior upon continuous shear stress. Results of in vitro and in vivo skin protection data have revealed that the active formulation has comparable skin protection effects to that of control formulation. It was presumed that stable multiple emulsions could be a promising choice for topical application of green tea but multiple emulsions presented in this study need improvement in the formula, concluded on the basis of pH, conductivity and apparent viscosity data.

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GREEN TEA EXTRACT IN AN EYELASH GROWTH ENHANCER GEL FORMULATION: STABILITY TEST, EYE IRRITATION TEST, AND HUMAN EYELASH GROWTH ACTIVITY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.18605 ◽

2017 ◽

Vol 10 (6) ◽

pp. 243

Author(s):

Indra Syahputra Roes Lie ◽

Joshita Djajadisastra ◽

Fadlina Chany Saputri

Keyword(s):

Green Tea ◽

Chorioallantoic Membrane ◽

Test Group ◽

Green Tea Extract ◽

Stability Test ◽

Eye Irritation ◽

Before And After ◽

Irritation Test ◽

Tea Extract

Objective: To formulate a green tea extract (GTE), which is often used as a hair growth product, to produce an eyelash gel with good stability, effectiveness, and safety for growing eyelashes.Methods: GTE was formulated into a gel. A stability test was performed at a high temperature (40±2°C), room temperature (25±2°C), low temperature (4±2°C), and a cycling temperature. An in vitro hen’s egg test-chorioallantoic membrane assay was performed to evaluate potential eye irritation. An eyelash growth test was conducted by length measurement using an eyelash ruler before and after 2 mo of application in human volunteers. Results: The GTE gel was stable in storage at high, room, and low temperatures and at cycling temperatures and did not cause eye irritation. Eyelashes grew significantly more in the test group than in the placebo group after 2 mo of application (p<0.05). Conclusion: GTE gel provides a new, safe, and effective option for growing natural eyelashes.

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