Application of a radical scavenging activity test to measure the total antioxidant activity of poultry meat

Meat Science ◽  
2008 ◽  
Vol 80 (4) ◽  
pp. 1081-1085 ◽  
Author(s):  
Giampiero Sacchetti ◽  
Carla Di Mattia ◽  
Paola Pittia ◽  
Giuseppe Martino
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Poultry Meat ◽  
Scavenging Activity ◽  
Total Antioxidant Activity ◽  
Activity Test ◽  
Total Antioxidant

Antioxidant Properties of Cashew Apple Wine (CAW)

2011 ◽  
Vol 396-398 ◽  
pp. 1645-1651 ◽  
Author(s):  
Qiu Ping Zhong ◽  
Yong Cheng Li
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Total Antioxidant Activity ◽  
Total Antioxidant ◽  
Reducing Capacity

In this study, total polyphenols content (TPC) and ascorbic acid content (AAC) in CAW were quantified. Total antioxidant activity, reducing capacity, and free radical scavenging activity of CAW were assayed. The TPC and AAC were 160 mg GAE /100ml and 132 mg/100 ml，respectivity. CAW exhibited effective antioxidant activities in the employed in vitro experiments, including total antioxidant activity, reducing capacity, and free radical scavenging activity, which indicates that phenolic compounds and ascorbic acid may play a key role in the antioxidant function of CAW.

scholarly journals Antioxidant activity of ethanolic extract of Penicillium chrysogenum and Penicillium fumiculosum

2014 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Jelica Stojanovic ◽  
Slavica Solujic ◽  
Miroslav Vrvic
Keyword(s):  
Antioxidant Activity ◽  
Penicillium Chrysogenum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Chelating Ability

The aim of this study was to investigate the biological and chemical activity on two species of fungi of the genus Penicillium isolated from wastewater. On the selected species of fungi the different antioxidant activity assays were carried out: DPPH free-radical scavenging activity, total antioxidant activity, Fe2+- chelating ability and Fe3+- reducing power. Total phenol content was also determinate for ethanolic extract of mycelia. Penicillium chrysogenum ethanolic extract contained higher total phenolic content and better total antioxidant capacity as well as ferrous ion chelating ability. Penicillium fumiculosum ethanolic extract showed higher DPPH free-radical scavenging activity, as well as reducing power. Based on the obtained results it can be concluded that two types of fungi are potential new sources of natural antioxidants.

scholarly journals In Vitro Inhibitory Effect on Carbohydrates Digestion and Antioxidant Potential of Garlic Scape (P06-076-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Dammah Otieno ◽  
Salma Altuwaijri ◽  
Hye Won Kang
Keyword(s):  
Antioxidant Activity ◽  
Digestive Enzymes ◽  
Radical Scavenging Activity ◽  
Health Effect ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Abstract Objectives Inhibition of carbohydrate absorption from intestine is an efficient way to prevent type 2 diabetes. Some edible plants have shown the potential as anti-diabetic agents by inhibiting carbohydrate-digestive enzymes in intestine. In terms of edible plant's health effect, bioactive compounds’ stability during the process of digestive enzymes has been commonly questioned. Therefore, the objectives of this study were to 1) examine inhibitory effect of garlic scape (GS), the green flower head of garlic on carbohydrate-degrading enzymes and 2) investigate antioxidant activity and total phenolic content (TPC) of GS treated with intestinal digestive enzymes. Methods GS extract (GSE) was prepared with 80% methanol (v/v) by homogenization, sonication, and filtration, and evaporation. α-glucosidase, maltase, glucoamylase, sucrase, α-amylase inhibitory activities were determined by measuring glucose hydrolyzed from nitrophenyl-glucopyranoside, maltose, starch, sucrose, and starch, respectively. To prepare gastrointestinal enzyme-digested GSE, GSE was digested with pepsin and a pancreatin-bile solution. GSE and gastrointestinal enzyme-digested GSE were assessed for TPC using a Folin-Ciocalteu reagent and for total antioxidant and radical scavenging activities by the following methods, phosphomolybdeum, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods, respectively. Results GSE (20 mg/ml) showed 30%, 50%, and 42% inhibition on α-glucosidase, maltase, and glucoamylase enzyme activities, respectively. GSE (50 mg/ml) showed 55.1% DPPH radical and 5782 mg vitamin C equivalent (VCE)/g ABTS radical scavenging activities. Total antioxidant activity of the GSE (1.5 mg/ml) exhibited 2.76 mg VCE/g. Consistent with its antioxidant property, the GSE showed a dose-dependent increase in TPC. Gastrointestinal enzymatic treatment resulted in approximately 46% reduction in DPPH and ABTS radical scavenging activity, total antioxidant activity, and TPC of the GSE. Conclusions Garlic scape has the potential as a natural anti-diabetic agent. Garlic scape keeps beneficial effects after the process of intestinal digestive system. Funding Sources The work was supported by USDA.

scholarly journals Formulation Evaluation and In- Vitro Antioxidant activity of Microparticles of Syzygium Cumini Plant Extract

2021 ◽  
pp. 77-85
Author(s):  
Sweta Srivastava Koka ◽  
Pravin Kumar Sharma ◽  
Vaishali Sharma ◽  
Jaya Verma ◽  
G. N. Darwhekar
Keyword(s):  
Antioxidant Activity ◽  
Plant Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Flowering Plant ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Antioxidant Supplement

Sygygium cumini, commonly known as Malabar plum, Java plum, black plum, jamun or jambolan, is an evergreen tropical tree in the flowering plant family Myrtaceae. The present study the antioxidant effects of microparticles of S. cumini plant extract were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay, total antioxidant activity and super oxide free radical scavenging activity. The microparticles showed different levels of radicals scavenging activity in a dose-dependent manner over the range of 50–150 µg/mL concentration, indicating the high antioxidative capacity of the extract. In all the experimental screening models microparticles of aqueous extract show the maximum inhibition of free radicals comparison of other experimental model at different concentrations. These findings suggest that the seed of Syginium cumini has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

scholarly journals Development and evaluation of nutritionally enhanced potato rice papads (Indian cookie)

2015 ◽  
Vol 7 (1) ◽  
pp. 242-248
Author(s):  
Sukhpreet Kaur ◽  
Poonam Aggarwal
Keyword(s):  
Antioxidant Activity ◽  
Solanum Tuberosum L ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Life Quality ◽  
Total Antioxidant Activity ◽  
Oil Uptake ◽  
Phytochemical Content ◽  
Overall Acceptability ◽  
Total Antioxidant

The present study was carried out to develop papads (Indian cookie) from potato (Solanum tuberosum L.) and rice (Oryza sativa) blends and to analyze them for organoleptic, physicochemical, phytochemical and shelf life quality. Two processing cultivars (Kufri Chipsona-1, Kufri Chandramukhi) and one commonly grown cultivar (Kufri Pukhraj) were evaluated for processing into papad. Based on preliminary sensory trails, papads with boiled potato mash (60%) and gelatinized rice (30%) level of supplementation were found to be most acceptable and these papads were subjected to nutritional evaluation. Results were compared with rice papads (control). Protein content and yield was significantly (p<0.05) higher in control papads compared to potato supplemented papads. Oil uptake significantly (p<0.05) increased on supplementation with potato. Bioactive compounds including ascorbic acid, totalphenolics and total antioxidant activity measured as DPPH radical scavenging activity increased significantly (p<0.05) on incorporation of potato. Between the cultivars, papads enriched with Kufri Pukhraj, a table variety which is considered unfit for processing, displayed the highest phytochemical content and total antioxidant activity. Sensory evaluation indicated higher overall acceptability scores of potato enriched papads compared to control. During storage of papads at room temperature for up to 3 months, significant (p<0.05) changes in the moisture content, phytochemical content and antioxidant activity were observed. Storage studies showed that the potato supplemented papads can be stored safely for 3 months of storage at ambient temperature.

Phytochemical Screening and In Vitro Antioxidant Activity of Ethanolic Extract of Cassia occidentalis

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
S. Srividya ◽  
G. Sridevi ◽  
A. G. Manimegalai
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Cassia Occidentalis ◽  
Total Antioxidant ◽  
Anti Oxidant

The ethanolic extract of the leaves of Cassia occidentalis (Co) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide (H2O2) radical scavenging activity, superoxide scavenging activity and Ferric reducing anti oxidant potential (FRAP). The analyses revealed that the ethanolic extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compared with the standard antioxidant ascorbic acid. The results have shown that crude ethanolic extract of the leaves of Co showed excellent antioxidant activity due to the presence of bioactive compounds namely alkaloids, betacyanin, cardiac glycosides, coumarins, flavonoids, phenols, steroids, saponins, tannins, terpenoids, anthraquinones and emodins.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.

2018 ◽  
Vol 11 (10) ◽  
pp. 433
Author(s):  
Rehana Banu H ◽  
Nagarajan N
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

scholarly journals An Investigation of free radical scavenging activity of various extracts of Olax scandens (family Olacaceae)

2020 ◽  
Vol 11 (1) ◽  
pp. 397-402
Author(s):  
Pranaya P ◽  
AkilaDevi D
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phosphomolybdic Acid ◽  
Scavenging Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Hydroxyl Radical Scavenging Activity ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

Olax scandens Roxb. (family Olacaceae) Available in throughout tropical India. The current study, aerial parts of different extracts of Olax scandens by different in-vitro methods. The antioxidant activity was evaluated by Hydroxyl radical scavenging activity(OH radical), Nitric oxide radical scavenging activity(NO radical), and total antioxidant activity (Phosphomolybdic acid method) with reference standard Ascorbic acid. An IC50 value was originated that methanolic extracts of Olax scandensis more efficient in Hydroxyl radical scavenging activity, Nitric oxide radical scavenging, total antioxidant activity than that of EA and petroleum ether extract. The methanolic  extracts  of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 253µg/ml & 135µg/ml (NO radical ), 205µg/ml & 57µg/ml (Phosphomolybdic acid method), 265µg/ml & 65µg/ml (OH radical) respectively. But when compared to all the three extracts with ascorbate (standard), the methanolic extract of the Olax scandens showed a better result. Moreover, the results were observed in a concentration-dependent manner. Invitro antioxidant studies obviously showed that methanolic extracts of Olax scandens have better antioxidant activity. These results indicate that aerial parts of methanolic concentrates Olax scandals could serve as a natural antioxidant, which may be useful to prevent free radical-induced diseases.

scholarly journals Antioxidant chlorophyll purification from maize leaves by liquid-to-liquid extraction method

2020 ◽  
Vol 10 (3) ◽  
pp. 152-158
Author(s):  
Dang Xuan Cuong
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Antioxidant Activity ◽  
Maize Leaves ◽  
Total Antioxidant

Chlorophyll is the blood of plant possesses a medical-value for treating support of disease in human, and the paper focused on the purification of antioxidant chlorophyll extracting from maize leaves in Vietnam. Chlorophyll was extracted with 96% ethanol and segmented by different solvents, for example, n-hexane, 96% ethanol, and ethyl acetate, respectively. Ethanol fraction was running via the chromatography column of silica gel for collecting antioxidant purified chlorophyll. All fractions were analysed chlorophyll content, antioxidant activities (total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity) and run the thin layer chromatography for determining the chlorophyll purification degree and the Rf. The results showed that the purification of antioxidant chlorophyll from maize leaves was by using the liquid-liquid segment and the column run, for example, in turn, ethanol, n-hexane, ethanol, and the silica gel chromatography. The highest value of chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity was 0.563±0.003 µg chl equivalent/ml, 1.392±0.018 mg ascorbic acid equivalent/ml, 3.396±0,024 mg FeSO4/ml, and 76.34±0.81 (%), respectively, as the n-hexane/ethanol ratio of 1/1 (v/v). After the chromatography run, chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity corresponded to 0.217±0.002 µg chl equivalent/ml, 0.628±0.013 mg ascorbic acid equivalent/ml, 1.928±0.019 mg FeSO4/ml, and 77.19±0.58 (%), respectively. Keywords: antioxidant, chlorophyll, maize, purification, liquid-to-liquid

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

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