Effects of low voltage electrostatic field on the microstructural damage and protein structural changes in prepared beef steak during the freezing process

Meat Science ◽  
2021 ◽  
pp. 108527
Author(s):  
Yong Xie ◽  
Bo Chen ◽  
Jie Guo ◽  
Wen Nie ◽  
Hui Zhou ◽  
...  
Energies ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2427
Author(s):  
Michał Szulborski ◽  
Sebastian Łapczyński ◽  
Łukasz Kolimas

The manuscript presents advanced coupled analysis: Maxwell 3D, Transient Thermal and Fluent CFD, at the time of a rated current occurring on the main busbars in the low-voltage switchgear. The simulations were procured in order to aid the design process of such enclosures. The analysis presented the rated current flow in the switchgear busbars, which allowed determining their temperature values. The main assumption of the simulation was measurements of temperature rise during rated current conditions. Simulating such conditions is a valuable asset in order to design better solutions for energy distribution gear. The simulation model was a precise representation of the actual prototype of the switchgear. Simulations results were validated by experimental research. The heat dissipation in busbars and switchgear housing through air convection was presented. The temperature distribution for the insulators in the rail bridge made of fireproof material was considered: halogen-free polyester. The results obtained during the simulation allowed for a detailed analysis of switchgear design and proper conclusions in practical and theoretical aspects. That helped in introducing structural changes in the prepared prototype of the switchgear at the design and construction stages. Deep analysis of the simulation results allowed for the development concerning the final prototype of the switchgear, which could be subjected to the full type tests. Additionally, short-circuit current simulations were procured and presented.


Author(s):  
Steven B. Herschbein ◽  
Kyle M. Winter ◽  
Carmelo F. Scrudato ◽  
Brian L. Yates ◽  
Edward S. Hermann ◽  
...  

Abstract Focused Ion Beam (FIB) chip circuit editing is a well-established highly specialized laboratory technique for making direct changes to the functionality of integrated circuits. A precisely tuned and placed ion beam in conjunction with process gases selectively uncovers internal circuitry, create functional changes in devices or the copper wiring pattern, and reseals the chip surface. When executed within reasonable limits, the revised circuit logic functions essentially the same as if the changes were instead made to the photomasks used to fabricate the chip. The results of the intended revision, however, can be obtained weeks or months earlier than by a full fabrication run. Evaluating proposed changes through FIB modification rather than proceeding immediately to mask changes has become an integral part of the process for bringing advanced designs to market at many companies. The end product of the FIB process is the very essence of handcrafted prototyping. The efficacy of the FIB technique faces new challenges with every generation of fabrication process node advancement. Ever shrinking geometries and new material sets have always been a given as transistor size decreases and overall packing density increases. The biggest fundamental change in recent years was the introduction of the FinFET as a replacement for the venerable planar transistor. Point to point wiring change methodology has generally followed process scaling, but transistor deletions or modifications with the change to Fins require a somewhat different approach and much more careful control due to the drastic change in height and shape. We also had to take into consideration the importance of the 4th terminal, the body-tie, that is often lost in backside editing. Some designs and FET technology can function acceptably well when individual devices are no longer connected to the bulk substrate or well, while others can suffer from profound shifts in performance. All this presents a challenge given that the primary beam technology improvements of the fully configured chip edit FIB has only evolved incrementally during the same time period. The gallium column system appears to be reaching its maximum potential. Further, as gallium is a p-type metal dopant, there are limitations to its use in close proximity to certain active semiconductor devices. Amorphous material formation and other damage mechanisms that extend beyond what can be seen visually when endpointing must also be taken into account [1]. Device switching performance and even transmission line characteristics of nearby wiring levels can be impacted by material structural changes from implantation cascades. Last year our lab participated in a design validation exercise in which we were asked to modify the drive of a multi-finger FinFET device structure to reduce its switching speed impact on a circuit. The original sized device pulled the next node in the chain too fast, resulting in a timing upset. Deleting whole structures and bridging over/around them is commonly done, but modifications to the physical size of an FET device is a rare request and generally not attempted. It requires a level of precision in beam control and post-edit treatment that can be difficult to execute cleanly. Once again during a complex edit task we considered the use of an alternate ion beam species such as neon, or reducing the beam energy (low kV) on the gallium tool. Unfortunately, we don’t yet have easy access to a versatile viable replacement column technology grafted to a fully configured edit station. And while there should be significantly reduced implant damage and transistor functional change when a gallium column FIB is operated at lower accelerating potential [2], the further loss of visual acuity due to the reduced secondary emission, especially when combined with ultra-low beam currents, made fast and accurate navigation near impossible. We instead chose the somewhat unconventional approach of using an ultra-low voltage electron beam to do much of the navigation and surface marking prior to making the final edits with the gallium ion beam in a dual-beam FIB tool. Once we had resolved how to accurately navigate to the transistors in question and expose half of the structure without disturbing the body-tie, we were able to execute the required cut to trim away 50% of the structure and reduce the effective drive. Several of the FIB modified units functioned per the design parameters of a smaller sized device, giving confidence to proceed with the revised mask set. To our surprise, the gallium beam performed commendably well in this most difficult task. While we still believe that an inert beam of similar characteristics would be preferable, this work indicates that gallium columns are still viable at the 14 nm FinFET node for even the most rigorous of editing requirements. It also showed that careful application of e-beam imaging on the exposed underside of FinFET devices could be performed without degrading or destroying them.


Author(s):  
Raghava Alapati ◽  
Dorel Moldovan ◽  
Ram V. Devireddy

In a typical cryopreservation protocol, the system to be preserved is first equilibrated with chemicals known as cryoprotective agents (CPAs). CPAs have been shown to alleviate cell damage from either the solute effects or the formation of intracellular ice during the subsequent freezing process. Thus, an extensive body of literature reporting the effects of CPAs on cellular systems has been accumulated over the last 50 years; detailing largely experimental interactions between cell systems and chemicals. Recent advances in computational methodology now offer an additional dimension in our ability to understand the molecular interactions between cell membranes, idealized as lipid bilayers and CPAs at atomistic scales. Computer simulations provide unique capabilities for analyzing biomembrane properties from atomistic perspective with a degree of detail that is hard to reach by other techniques. The excellent agreement with the experiment obtained in various molecular dynamics (MD) studies [1] on simple model membranes has raised the confidence in applying the molecular dynamics simulations to even more complex systems. Dimethylsulfoxide (DMSO) is one of the most widely used solvents in cell biology and cryopreservation. During a typical cryopreservation protocol the DMSO composition of aqueous buffers inside and outside of the cell is known to differ considerably. To model and understand the structural changes in cell membranes in such a situation we performed MD simulations of an idealized lipid bilayer membrane which separates two aqueous reservoirs with and without DMSO. Zwitterionic dimyritoylphosphatidylcholine (DMPC) lipid bilayers was chosen as the model membrane.


Author(s):  
I. Müllerová ◽  
M. Lenc

The advantages of the LV SEM are well known. Recently a lot of interesting results from this field were presented which were obtained thanks to development of field emission guns and to the enourmous progress in the computation techniques in electron optics.One of the simplest arrangements of the LVSEM is shown in Figure 1. The Tesla SEM BS 350 with a field emission gun and the TF-W/100-Zr cathode was used for our experiment. The gun provides 10−10 A current in the diffraction limited spot (for the angular density 0.20mA sr−1). If a potential Usp is applied to the specimen the energy E of the electrons that strike the specimen is Ep-eUsp (Ep-primary beam energy, e-elementary charge). The produced secondary (SE) and backscattered (BSE) electrons are accelerated towards the semiconductor detector by the electrostatic field and their energy spectrum extends from eUsp to Ep. The final energy of the SE and BSE can then be sufficient for achieving a reasonable amplification of the semiconductor detector which is directly proportional to the energy of the electrons that strike the detector. We calculated optical properties for a combination of the electrostatic and magnetic lenses of the basic geometry shown in Figure 1 and for an arrangement with the single polepiece lens shown in Figure 2. We particularly investigated coefficients of the chromatic (Cc) and spherical (Cs) aberrations as functions of the ratio of the primary beam energy to the energy of the electrons that strike the specimen Ep/E for some optimum position of the specimen, electropstatic and magnetic field. Our results are shown in Table 1. The coefficients Cs and Cc do not change with the energy Epor E if the ratio Ep/E is maitained the same and aberrations are lower for larger ratios Ep/E, so that the influence of the contribution of the electrostatic lens aberrations is negligible for our geometry. For example, if we require a resolution limit r=2nm and an energy of the electrons that strike the specimen E=300eV, it is possible to calculate that the coefficient of the aberrations must be Cs<0.21mm and Cc<0.14mm for an energy width AE=0.2eV, so that we need the ratio Ep/E≥150 for the arrangement shown in Figure 1 (i.e.Ep≥45keV) and Ep/E≥33 for the arrangement shown in Figure 2 (i.e.Ep≥10keV).The advantages of the combination of the magnetic lens with the electrostatic cathode lens for the high resolution very low energy electron microscopy are well known . We assume that for the LVSEM only a medium electrostatic field strength is admitted at the specimen surface. Nevertheless, our experimental arrangements should certainly be optimized in the future.


2020 ◽  
Author(s):  
Sophie Bogler ◽  
Nadine Borduas-Dedekind

&lt;p&gt;Uncertainties in current predictions for the atmosphere&amp;#8217;s radiative balance are dominated by the impact of clouds. Ice nucleating particles (INPs) play a dominant role in the formation of mixed-phase clouds, however there is still a lack of understanding of how INPs interact with water in the freezing process. Detailed elucidations of the organic aerosol chemical composition from IN active atmospheric samples are scarce which is due to the analytical challenge of resolving their high complexity. We chose to reduce sample complexity by investigating the IN activity of a specific sub-component of organic aerosols, the biopolymer lignin. This approach facilitates connecting ice nucleating abilities to molecular properties. Ice nucleation experiments were conducted in our home-built Freezing Ice Nuclei Counter (FINC) to measure freezing temperatures in the immersion freezing mode which is the dominant IN mechanism in mixed-phase clouds. We find that lignin acts as an INP at temperatures relevant for mixed-phase cloud processes (e.g. 50% activated fraction at &amp;#8211; 20 &amp;#176;C concentrated 20 mg C/L). Photochemistry and ozonation experiments were subsequently conducted to test the effect of atmospheric processing on lignin&amp;#8217;s IN activity. We discovered that this activity was not susceptible to change under environmentally relevant conditions even though structural changes were introduced by monitoring UV/Vis absorbance. Additionally to atmospheric processing, laboratory treatments including heating, sonication and oxidation with hydrogen peroxide were done, where only the heating experiments had a decreasing effect on lignin&amp;#8217;s IN activity.&amp;#160; Based on these results, we present a thorough INP characterization of lignin, a specific organic matter subcomponent, and contribute to the understanding of how organic material present in the atmosphere can nucleate ice.&lt;/p&gt;


Chemosphere ◽  
2021 ◽  
Vol 264 ◽  
pp. 128378
Author(s):  
Yi Yang ◽  
Kok Yuen Koh ◽  
Haoxin Huang ◽  
Huiping Zhang ◽  
Ying Yan ◽  
...  

2020 ◽  
Vol 74 (9) ◽  
pp. 1155-1160 ◽  
Author(s):  
George Sarau ◽  
Lasse Kling ◽  
Barbara E. Oßmann ◽  
Ann-Katrin Unger ◽  
Frank Vogler ◽  
...  

Microplastics (MPs) have been reported in various environmental compartments and their number is continuously increasing because of degradation into smaller fragments down to nanoplastics. Humans are exposed to these small-sized MPs through food and air with potential health consequences that still need to be determined. This requires, in the first place, efficient and detailed visualization, relocalization, and characterization of the same MPs with complementary analytical methods. Here, we show the first application of a correlative microscopy and spectroscopy workflow to MPs that meets these demands. For this purpose, standard MP particles on aluminum-coated polycarbonate membrane filters were investigated by an optical zoom microscope and a hyphenated scanning electron microscopy (SEM)-Raman system. By merging the obtained data in one software, it is possible to navigate on the entire filters’ surface and correlate at identical locations MP morphology at the spatial resolutions of electron (1.6 nm at 1 kV for the used SEM, ∼100 nm minimum MP size in this study) and optical (∼1–10 µm) microscopies with chemical identification by micro-Raman spectroscopy. Moreover, we observed that low-voltage SEM works without a conductive coating of MPs, causes no detectable charging and structural changes, and provides high-resolution surface imaging of single and clustered MP particles, thus enabling subsequent Raman measurements. We believe that further work on the accurate identification and quantification of micro- and nanoplastics in real samples can potentially profit from this workflow.


2021 ◽  
Vol 1790 (1) ◽  
pp. 012061
Author(s):  
Shuiqing Zhou ◽  
Qiusheng Wang ◽  
Shixian Chen ◽  
Zhanao Hu ◽  
Shuqin Yan ◽  
...  

2021 ◽  
Vol 59 (11) ◽  
pp. 796-801
Author(s):  
Jin-Ju Choi ◽  
Byoungyong Im ◽  
Yubin Kang ◽  
Dae-Geun Kim

Compressed wires are produced by cross-sectionally compressing stranded conductors, which results in a smaller conductor diameter. This also leads to a lower weight wire, because a thinner external insulated coating can be used, compared to the low-voltage wires typically used in automobiles. However, a post production heat treatment of the compressed wires is required because plastic deformation occurs during compression after drawing the wires. In this study, the work hardening of stranded compressed copper wires was controlled by Joule-heating, and the resulting changes in microstructure, mechanical, and electrical properties after various annealing voltages (0, 25, 27, 31, 35, and 39 V) were observed. The results confirmed that as the annealing voltage increased from 0 to 31 V, the anisotropic deformation texture with a <111> orientation as the main component was reduced, and micrograins were generated throughout the stranded wires via recrystallization. At an annealing voltage above 31 V, the grains grew to be more than twice as large as those before heat treatment. At an annealing voltage of 31 V these structural changes contribute to the elongation increase of the compressed wires to 28.34%, and an improvement in electrical resistance to 145.85 mΩ.


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