scholarly journals Development of a multilocus sequence typing tool for high-resolution subtyping and genetic structure characterization of Cryptosporidium ubiquitum

2016 ◽  
Vol 45 ◽  
pp. 256-261 ◽  
Author(s):  
Ying Tang ◽  
Na Li ◽  
Mingxin Song ◽  
Dawn M. Roellig ◽  
Yaoyu Feng ◽  
...  
Keyword(s):  
Genetic Structure ◽  
High Resolution ◽  
Multilocus Sequence Typing ◽  
Structure Characterization ◽  
Typing Tool

scholarly journals Common Phase and Structure Misidentifications in High-Resolution TEM Characterization of Perovskite Materials

2020 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Yu-Hao Deng
Keyword(s):  
High Resolution ◽  
Research Field ◽  
Structure Characterization ◽  
Phase Identification ◽  
Lead Iodide ◽  
Perovskite Materials ◽  
High Resolution Tem ◽  
Methylammonium Lead Iodide ◽  
Dose Imaging

High-resolution TEM (HRTEM) is a powerful tool for structure characterization. However, methylammonium lead iodide (MAPbI3) perovskite is highly sensitive to electron beams and easily decomposes into lead iodide (PbI2). Misidentifications, such as PbI2 being incorrectly labeled as perovskite, are widely present in HRTEM characterization and would negatively affect the development of perovskite research field. Here misidentifications in MAPbI3 perovskite are summarized, classified, and corrected based on low-dose imaging and electron diffraction (ED) simulations. Corresponding crystallographic parameters of intrinsic tetragonal MAPbI3 and the confusable hexagonal PbI2 are presented unambiguously. Finally, the method of proper phase identification and some strategies to control the radiation damage in HRTEM are provided. This warning paves the way to avoid future misinterpretations in HRTEM characterization of perovskite and other electron beam-sensitive materials.

scholarly journals 3-Alkylpyridinium salts from Haplosclerida marine sponges: Isolation, structure elucidations, and biosynthetic considerations

2009 ◽  
Vol 81 (6) ◽  
pp. 1033-1040 ◽  
Author(s):  
Rémi Laville ◽  
Philippe Amade ◽  
Olivier P. Thomas
Keyword(s):  
High Resolution ◽  
Mass Spectra ◽  
Biosynthetic Pathway ◽  
2D Nmr ◽  
Marine Sponges ◽  
Structure Characterization ◽  
Ionization Mass ◽  
Family Structures ◽  
Resolution Electron

A very little studied marine sponge Callyspongia sp. collected off the coast of Martinique was chemically investigated. The study led to the isolation and structure characterization of two new 3-alkylpyridinium salts which belonged to the recently isolated pachychaline family. Structures were elucidated by 1D, 2D NMR and detailed high-resolution electron spray ionization mass spectra (HRESIMS)-MS studies. The use of HRESIMS-MS studies proved to be highly efficient to identify two other close derivatives in a mixture. Finally, these studies allowed us to propose a general biosynthetic pathway leading to important 3-alkylpyridinium salts.

High-resolution electron microscopy on SSZ-26 zeolite materials

1991 ◽  
Vol 49 ◽  
pp. 1032-1033 ◽  
Author(s):  
M. Pan ◽  
P.A. Crozier ◽  
I.Y. Chan ◽  
S.I. Zones
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Imaging Techniques ◽  
High Resolution Electron Microscopy ◽  
Shape Selectivity ◽  
Structure Characterization ◽  
Resolution Electron ◽  
Structure Sensitive ◽  
Dose Imaging

Zeolites constitute an important class of catalyst materials in the petrochemical industry. Zeolites are aluminosilicates with their framework structures made up of SiO4 (or AlO4) tetrahedra. The catalytic properties such as selectivity and activity are closely related to the structure sensitive properties of zeolites, e.g. shape selectivity, diffusivity, etc. Understanding these properties requires detailed structure characterization of the zeolites. High resolution electron microscopy (HREM) has been successfully applied to solve some zeolite structures when combined with experimental data from other techniques, e.g. absorption experiment. The connectivities of n-member rings in the zeolite frameworks can be directly deduced from the corresponding HREM images. However, radiation damage to the zeolite framework structures by the electron beam necessitates the use of low dose imaging techniques.

scholarly journals Transmission electron microscopy as an important tool for characterization of zeolite structures

2018 ◽  
Vol 5 (11) ◽  
pp. 2836-2855 ◽  
Author(s):  
W. Wan ◽  
J. Su ◽  
X. D. Zou ◽  
T. Willhammar
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Electron Diffraction ◽  
Electron Tomography ◽  
Structure Characterization ◽  
Transmission Electron ◽  
High Resolution Tem

This review presents various TEM techniques including electron diffraction, high-resolution TEM and scanning TEM imaging, and electron tomography and their applications for structure characterization of zeolite materials.

scholarly journals Genetic structure characterization of Chileans reflects historical immigration patterns

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Susana Eyheramendy ◽  
Felipe I. Martinez ◽  
Federico Manevy ◽  
Cecilia Vial ◽  
Gabriela M. Repetto
Keyword(s):  
Genetic Structure ◽  
Structure Characterization

scholarly journals Development of a Multilocus Sequence Typing Tool for High-Resolution Genotyping of Enterocytozoon bieneusi

2011 ◽  
Vol 77 (14) ◽  
pp. 4822-4828 ◽  
Author(s):  
Yaoyu Feng ◽  
Na Li ◽  
Theresa Dearen ◽  
Maria L. Lobo ◽  
Olga Matos ◽  
...  
Keyword(s):  
High Resolution ◽  
Multilocus Sequence Typing ◽  
Enterocytozoon Bieneusi ◽  
Whole Genome Sequence ◽  
Rrna Gene ◽  
Content Type ◽  
Health Significance ◽  
The One ◽  
Pcr Targeting ◽  
Typing Tool

ABSTRACTThus far, genotyping ofEnterocytozoon bieneusihas been based solely on DNA sequence analysis of the internal transcribed spacer (ITS) of the rRNA gene. Both host-adapted and zoonotic (human-pathogenic) genotypes ofE. bieneusihave been identified. In this study, we searched for microsatellite and minisatellite sequences in the whole-genome sequence database ofE. bieneusiisolate H348. Seven potential targets (MS1 to MS7) were identified. Testing of the seven targets by PCR using two human-pathogenicE. bieneusigenotypes (A and Peru10) led to the selection of four targets (MS1, MS3, MS4, and MS7). Further analysis of the four loci with an additional 24 specimens of both host-adapted and zoonoticE. bieneusigenotypes indicated that most host-adapted genotypes were not amplified by PCR targeting these loci. In contrast, 10 or 11 of the 13 specimens of the zoonotic genotypes were amplified by PCR at each locus. Altogether, 12, 8, 7, and 11 genotypes of were identified at MS1, MS3, MS4, and MS7, respectively. Phylogenetic analysis of the nucleotide sequences obtained produced a genetic relationship that was similar to the one at the ITS locus, with the formation of a large group of zoonotic genotypes that included mostE. bieneusigenotypes in humans. Thus, a multilocus sequence typing tool was developed for high-resolution genotyping ofE. bieneusi.Data obtained in the study should also have implications for understanding the taxonomy ofEnterocytozoonspp., the public health significance ofE. bieneusiin animals, and the sources of humanE. bieneusiinfections.

scholarly journals The High Resolution Diffraction Beamline P08 at Petra III Expanded Towards a Platform for Structure Characterization of Organic Liquid Surfaces - Results from Lipid Monolayers

2018 ◽  
Vol 114 (3) ◽  
pp. 524a ◽  
Author(s):  
Florian Bertram ◽  
Gerald Brezesinski ◽  
Olof Gutowski ◽  
Beate Klösgen ◽  
Milena Lippmann ◽  
...  
Keyword(s):  
High Resolution ◽  
Organic Liquid ◽  
Structure Characterization ◽  
Lipid Monolayers ◽  
Liquid Surfaces

Tem Structure Characterization Of Ti/Al and Ti/Al/Ni/Au Ohmic Contacts For n-GaN

1996 ◽  
Vol 423 ◽  
Author(s):  
S. Ruvimov ◽  
Z. Liliental-Weber ◽  
J. Washburn ◽  
K. J. Duxstad ◽  
E. E. Hailer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Ohmic Contacts ◽  
High Resolution Electron Microscopy ◽  
Structure Characterization ◽  
Transmission Electron ◽  
Resolution Electron ◽  
Thin Polycrystalline

AbstractTransmission electron microscopy has been applied to characterize the structure of Ti/Al and Ti/Al/Ni/Au ohmic contacts on n-type GaN (˜1017 cm−3 ) epitaxial layers. A thin polycrystalline cubic TiN layer epitaxially matched to the (0001) GaN surface was detected at the interface with the GaN substrate. This layer was studied in detail by electron diffraction and high resolution electron microscopy. The orientation relationship between the cubic TiN and the GaN was found to be: {111}TiN//{00.1}GaN, [110]TiN//[11.0]GaN, [112 ]TiN//[ 10.0]GaN. The formation of this cubic TiN layer results in an excess of N vacancies in the GaN close to the interface which is considered to be the reason for the low resistance of the contact.

scholarly journals Establishment of a Publicly Available Core Genome Multilocus Sequence Typing Scheme for Clostridium perfringens

2021 ◽  
Author(s):  
Mostafa Y. Abdel-Glil ◽  
Prasad Thomas ◽  
Jörg Linde ◽  
Keith A. Jolley ◽  
Dag Harmsen ◽  
...  
Keyword(s):  
High Resolution ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Core Genome ◽  
Epidemiological Surveillance ◽  
Whole Genome ◽  
Bacterial Strains

Global epidemiological surveillance of bacterial pathogens is enhanced by the availability of standard tools and sharing of typing data. The use of whole-genome sequencing has opened the possibility for high-resolution characterization of bacterial strains down to the clonal and subclonal levels.

Skeletal elements of crinoid echinoderms: High-resolution structural and microanalytical results

1983 ◽  
Vol 41 ◽  
pp. 194-195
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor
Keyword(s):  
High Resolution ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Structural Features ◽  
Sea Stars ◽  
High Resolution Tem ◽  
Relationship Of ◽  
The Relationship ◽  
Insight Into

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.

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