Assessment of antibacterial efficacy of a biocompatible nanoparticle PC@AgNPs against Staphylococcus aureus

ABSTRACT This study investigated the in vivo efficacy of three bacteriophages combined compared with linezolid in two mouse models (nondiabetic and diabetic) of Staphylococcus aureus foot infection. In both models, a single injection of bacteriophages in the hindpaw showed significant antibacterial efficacy. Linezolid was as effective as bacteriophages in nondiabetic animals but ineffective in diabetic animals. These findings further support preclinical and clinical studies for the development of phage therapy.

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Potential antibacterial efficacy of garlic extract on Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae: An In vitro study

Journal of Pharmacy And Bioallied Sciences ◽

10.4103/jpbs.jpbs_681_20 ◽

2021 ◽

Vol 13 (5) ◽

pp. 590

Author(s):

Mohammed Abidullah ◽

Pradnya Jadhav ◽

SSri Sujan ◽

AyyapaGandhi Shrimanikandan ◽

ChukkaRakesh Reddy ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Klebsiella Pneumoniae ◽

In Vitro Study ◽

Garlic Extract ◽

Vitro Study ◽

Antibacterial Efficacy

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Green synthesis of silver nanoparticles using aqueous extract of Citrus sinensis peels and evaluation of their antibacterial efficacy

Green Processing and Synthesis ◽

10.1515/gps-2021-0061 ◽

2021 ◽

Vol 10 (1) ◽

pp. 851-859

Author(s):

Lebogang Mogole ◽

Wesley Omwoyo ◽

Elvera Viljoen ◽

Makwena Moloto

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Citrus Sinensis ◽

Antimicrobial Activities ◽

Antibacterial Efficacy ◽

Gram Positive ◽

Gram Negative ◽

Average Size ◽

Passivating Agent

Abstract The resistance of microorganisms towards antibiotics remains a big challenge in medicine. Silver nanoparticles (AgNPs) received attention recently for their characteristic nanosized features and their ability to display antimicrobial activities. This work reports the synthesis of AgNPs using the Citrus sinensis peels extract in their aqueous, mild, and less hazardous conditions. The effect of concentration variation (1%, 2%, and 3%) of the plant extracts on the size and shape of the AgNPs was investigated. The antimicrobial activities were tested against gram-positive Staphylococcus aureus and gram-negative Klebsiella pneumoniae. Absorption spectra confirmed the synthesis by the surface Plasmon resonance peaks in the range 400–450 nm for all the AgNPs. FTIR spectra confirmed that Citrus sinensis peels extract acted as both reducing and surface passivating agent for the synthesized AgNPs. TEM revealed spherical AgNPs with average size of 12 nm for 3% concentration as compared to the agglomeration at 1% and 2%. All the AgNPs synthesized using Citrus sinensis peels extracts (1%, 2%, and 3%) exhibited antimicrobial activity against both gram-positive and negative bacteria. These results indicated a simple, fast, and inexpensive synthesis of silver nanoparticles using the Citrus sinensis peels extract that has promising antibacterial activity.

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THE ANTIBACTERIAL EFFICACY OF ETHANOL EXTRACTS OBTAINED FROM LEAVES OF SOME THYMUS SPECIES (LAMIACEAE) AGAINST STAPHYLOCOCCUS AUREUS SUBSP. AUREUS STRAIN

The Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine ◽

10.32900/2312-8402-2021-125-18-29 ◽

2021 ◽

pp. 18-29

Author(s):

N. Kurhaluk ◽

H. Tkachenko ◽

V. Honcharenko ◽

V. Nachychko ◽

A. Prokopiv ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activities ◽

Aureus Strain ◽

Future Research ◽

Antibacterial Efficacy ◽

Disk Diffusion Assay ◽

The Difference ◽

Plant Families ◽

Ethanol Extracts

A convincing number of investigations that indicate that thymol with other metabolites exhibited potent antimicrobial, antifungal, antibacterial, and antiparasitic properties prompted us to verify the antibacterial efficacy of four species and one interspecific hybrid of the Thymus genus collected in the western part of Ukraine against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Considering the points highlighted above and based on previous results obtained in our laboratory, in the current study, we decided to evaluate the antimicrobial efficacy of five ethanol extracts obtained from leaves of Thymus representatives against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Fresh leaves were washed, weighed, crushed, and homogenized in 96 % ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. Antimicrobial activity was determined using the agar disk diffusion assay. The ethanol extracts obtained from leaves of Thymus plants showed different antibacterial activities against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. The antibacterial activity of extracts was greatest for Th. serpyllum followed by Th. pannonicus followed by Th. pulegioides, Th. alpestris, and then by Th. x porcii. Since the antibacterial effectiveness of medicinal plants varies dramatically depending on the phytochemical characteristics of plant families and subfamilies, it is not surprising to note the difference in this efficacy even when using samples taken from the same plant, but from two different regions. The antimicrobial activity of the crude ethanol extracts obtained from leaves of Thymus plants may be attributed to specific compounds or a combination of compounds. The present study lays the basis for future research, to validate the possible use of Thymus species as a candidate in the treatment of infections caused by Staphylococcus aureusin medicine and veterinary.

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