scholarly journals THE ANTIBACTERIAL EFFICACY OF ETHANOL EXTRACTS OBTAINED FROM LEAVES OF SOME THYMUS SPECIES (LAMIACEAE) AGAINST STAPHYLOCOCCUS AUREUS SUBSP. AUREUS STRAIN

2021 ◽  
pp. 18-29
Author(s):  
N. Kurhaluk ◽  
H. Tkachenko ◽  
V. Honcharenko ◽  
V. Nachychko ◽  
A. Prokopiv ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activities ◽  
Aureus Strain ◽  
Future Research ◽  
Antibacterial Efficacy ◽  
Disk Diffusion Assay ◽  
The Difference ◽  
Plant Families ◽  
Ethanol Extracts

A convincing number of investigations that indicate that thymol with other metabolites exhibited potent antimicrobial, antifungal, antibacterial, and antiparasitic properties prompted us to verify the antibacterial efficacy of four species and one interspecific hybrid of the Thymus genus collected in the western part of Ukraine against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Considering the points highlighted above and based on previous results obtained in our laboratory, in the current study, we decided to evaluate the antimicrobial efficacy of five ethanol extracts obtained from leaves of Thymus representatives against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Fresh leaves were washed, weighed, crushed, and homogenized in 96 % ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. Antimicrobial activity was determined using the agar disk diffusion assay. The ethanol extracts obtained from leaves of Thymus plants showed different antibacterial activities against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. The antibacterial activity of extracts was greatest for Th. serpyllum followed by Th. pannonicus followed by Th. pulegioides, Th. alpestris, and then by Th. x porcii. Since the antibacterial effectiveness of medicinal plants varies dramatically depending on the phytochemical characteristics of plant families and subfamilies, it is not surprising to note the difference in this efficacy even when using samples taken from the same plant, but from two different regions. The antimicrobial activity of the crude ethanol extracts obtained from leaves of Thymus plants may be attributed to specific compounds or a combination of compounds. The present study lays the basis for future research, to validate the possible use of Thymus species as a candidate in the treatment of infections caused by Staphylococcus aureusin medicine and veterinary.

Synthesis and antimicrobial activity of 4-trifluoromethylpyridine nucleosides

2017 ◽  
Vol 23 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Shaikha S. Alneyadi ◽  
Anas A. Abdulqader ◽  
Alaa A. Salem ◽  
Ibrahim M. Abdou
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Stenotrophomonas Maltophilia ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Nucleoside Analogues ◽  
Reference Drug ◽  
Minimum Inhibitory Concentrations

Abstract4-Trifluoromethylpyridine derivatives 4–8 represent good candidates for the discovery of new antibacterial agents. Fluorinated pyridine nucleosides 4–7 and non-nucleoside analogues 8a,b were synthesized and evaluated for their antibacterial activities against Staphylococcus aureus, Bacillus infantis, Escherichia coli and Stenotrophomonas maltophilia. The minimum inhibitory concentrations (MICs) of the new nucleosides 4–7 range from 1.3 to 4.9 μg/mL and MICs of fluoroaryl derivatives 8a,b are in the range of 1.8–5.5 μg/mL. Activity of amoxicillin, the reference drug, is 1.0–2.0 μg/mL under similar conditions.

scholarly journals Antioxidant and Antibacterial Activities of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Halima Rabib ◽  
Chaimaa Elagdi ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
Tayeb Koussa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Bornyl Acetate ◽  
Radical Trap ◽  
Tetraclinis Articulata

The purpose of this study is to evaluate and compare the antioxidant and antibacterial activities of essential oil isolated from Tetraclinis articulata (Vahl) leaves, Masters originating in Morocco (Benslimane Region, Atlantic-influenced plain). The analysis of the major compounds of essential oil was performed by gas chromatography and mass spectrometry, and this oil is dominated by bornyl acetate (35.05%), camphor (11.17%), and α-pinene (10.84%). The antioxidant properties were evaluated by the test of the radical trap 2,2-diphényl-1-picrylhydrazyl (DPPH), and the antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli which have been inhibited from the 25 μg/mL.

Antimicrobial Activity of Extracts of the Lichen Xanthoparmelia pokornyi and its Gyrophoric and Stenosporic Acid Constituents

2006 ◽  
Vol 61 (5-6) ◽  
pp. 319-323 ◽  
Author(s):  
Mehmet Candan ◽  
Meral Yılmaz ◽  
Turgay Tay ◽  
Merih Kıvança ◽  
Hayrettin Türk
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Aeromonas Hydrophila ◽  
Candida Glabrata ◽  
Proteus Vulgaris ◽  
Ethanol Extracts ◽  
Bacteria And Fungi ◽  
Gyrophoric Acid

The antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen Xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. Both the extracts and the acids showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Proteus vulgaris, Staphylococcus aureus, Streptococcus faecalis, Yersinia enterocolitica, Candida albicans and Candida glabrata. The extracts were inactive against the tested filamentous fungi. The MIC values of the extracts and the acids for the bacteria have also been determined.

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

2003 ◽  
Vol 9 (1) ◽  
pp. 49-51
Author(s):  
Farida Lanawati Darsono ◽  
Stephanie Devi Artemisia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Tween 80 ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Positive Bacteria ◽  
Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

scholarly journals SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SOME 1,4- AND 1,4,5-SUBSTITUTED-1H-1,2,3TRIAZOLES IN RELATION TO THE STAPHYLOCOCCUS AUREUS STRAIN

2018 ◽  
Vol 62 (3) ◽  
pp. 293-297 ◽  
Author(s):  
A. V. Zuraev ◽  
A. V. Lishai ◽  
Yu. V. Grigoriev ◽  
O. A. Ivashkevich
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Catalytic System ◽  
Bactericidal Activity ◽  
Cycloaddition Reaction ◽  
Antibacterial Activities ◽  
Aureus Strain ◽  
Derivatives Of

A number of 1,4- and 1,4,5-triazoles have been synthesized using a modified catalytic system for the Huisgen [3+2] cycloaddition reaction. The bactericidal activity of the synthesized compounds has been studied. The synthesized derivatives of 1H-1,2,3-triazoles have demonstrated higher antibacterial activities in relation to the pathogen Staphylococcus aureus strain which can be comparable with the clinically used antibiotic “Cefotaxime”.

scholarly journals Aktivitas Antibakteri Sabun Cuci Tangan yang Mengandung Ekstrak Metanol Rumput Laut Eucheuma spinosum (Antibacterial Activity Test of Eucheuma spinosum Methanol Extract Hand Wash)

MEDULA ◽  
2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Nur Illiyyin Akib ◽  
Mariani Triwatami ◽  
Andi Eka Purnama Putri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
E Coli ◽  
Hand Wash ◽  
Ethanol Extracts ◽  
Physical And Chemical

Background: Eucheuma spinosum seaweed contain flavonoid, triterpenoid, alkaloids and polyphenol which has been widely used in antibacterial activity. Purpose(s):The aim of the research are to determine antibacterial activities of metanol extracts of E. spinosum against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922; to formulate hand wash of seaweed of methanol extract E. spinosum that has antibacterial activity and has physical and chemical stability. Methods: Seaweed E.spinosum methanol extract was derived by maceration method. Antibacterial activities of the extract were tested by liquid dilution and solid dilution method. Hand wash was formulated by mechanical dissolved methods. Antibacterial activity of hand wash were tested by liquid dilution and solid dilution method. Physical and chemical stabilities were conducted by cycling test. Results:. These were showed through minimum inhibitory concentration (MIC) of ethanol extracts of E. spinosum against S. aureus ATCC 25923 at concentrations of 6% and E. coli ATCC 25922 at concentrations of 6%. The minimum bactericidal concentration (MBC) of ethanol extracts of E. spinosum against S. aureus ATCC 25923 at concentrations of 8% and E. coli ATCC 25922 at concentrations of 8%. Formulation of hand wash seaweed E. spinosum methanol extract at concentrations of 8% and 10%. The test of stabilities results of hand wash changes were organoleptic, viscosity, pH, and foaming ability were accordance to qualified standard. The antibacterial activity of hand wash contains seaweed E. spinosum methanol extract at concentration of  8% and 10% have bactericidal activity againts S. aureus ATCC 25923 and E. coli ATCC 25922. Conclusion: Overall, these results suggested that formula of hand wash contains E. spinosum metanol extracts have antibacterial properties against S. aureus ATCC 25923 and E. coli ATCC 25922.Keywords: antibacterial, Eucheuma spinosum, hand wash, physical and chemical stability Latar Belakang: Rumput laut Eucheuma spinosum mengandung flavonoid, triterpenoid, alkaloid, dan polifenol yang memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak metanol E. spinosum terhadap Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922; membuat sediaan sabun cuci tangan dari ekstrak metanol E. spinosum yang memiliki aktivitas antibakteri dan stabil secara fisika dan kimia. Metode: Ekstrak metanol E. spinosum diperoleh dengan metode maserasi. Uji aktivitas antibakteri ekstrak dilakukan dengan metode dilusi cair dan dilusi padat. Sabun cuci tangan diformulasi dengan metode pencampuran mekanik. Uji aktivitas antibakteri sabun cuci tangan dilakukan dengan metode dilusi cair dan dilusi padat. Uji stabilitas fisika kimia dilakukan dengan metodecycling test. Hasil: Konsentrsi Hambat Minimum (KHM) ekstrak metanol E. spinosum terhadap S. aureus ATCC 25923 adalah 6% dan terhadap E. coli ATCC 25922 adalah 8%. Ekstrak metanol E. spinosum dapat diformulasi menjadi sabun cuci tangan dengan konsentrasi 8% dan 10%. Uji stabilitas menunjukkan bahwa perubahan organoleptik, viskositas, pH, dan kemampuan membentuk busa masih berada dalam nilai yang dipersyaratkan. Uji aktivitas antibakteri sabun cuci tangan yang mengandung ekstrak metanol E. spinosum dengan konsentrasi 8% dan 10% memiliki aktivitas antibakteri terhadap ATCC 25923 and E. coli ATCC 25922. Simpulan: Formula sabu cuci tangan yang mengandung ekstrak metanol E. spinosum memiliki aktivitas antibakteri terhadap ATCC 25923 and E. coli ATCC 25922.Kata kunci: antibakteri, Eucheuma spinosum, sabun cuci tangan, stabilitas fisika kimia

scholarly journals AKTIVITAS ANTIMIKROBA DARI EKSTRAK DAN FRAKSI ORGANISME LAUT SPONS Stylissa carteri YANG DIKOLEKSI DARI PERAIRAN SELAT LEMBEH KOTA BITUNG

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 662
Author(s):  
Cavieta C. A. Watupongoh ◽  
Defny S. Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Extraction Process ◽  
Marine Biota ◽  
Active Compounds ◽  
Stylissa Carteri ◽  
Agar Method ◽  
Ethanol Extracts

ABSTRACTSponge is a multi-cell marine biota whose tissue and organ functions are very simple. Sponges have considerable potentially in producing active compounds that can be used in the pharmaceutical world. This study aimed to determine the presence of antimicrobial activity from extracts and fractions of the Stylissa carteri Sponge on against the microbes of Staphylococcus aureus, Escherichia coli, and Candida albicans. The extraction process was carried out by maceration using ethanol solvent, and fractionation was carried out using methanol, n-hexane and chloroform solvents. Antimicrobial activity was carried out by disk diffusion agar method. The results showed that the crude ethanol extracts and methanol fractions of the Stylissa carteri sponge actively inhibited the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans microbes. Keywords: Stylissa carteri, antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albicans.  ABSTRAKSpons merupakan biota laut multi sel yang fungsi jaringan dan organnya sangat sederhana. Spons memiliki potensi cukup besar dalam  menghasilkan senyawa aktif yang dapat digunakan dalam dunia farmasi. Penelitian ini bertujuan untuk menentukan adanya Aktivitas Antimikroba dari ekstrak dan fraksi Spons Stylissa carteri terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans. Dilakukan proses ekstraksi dengan cara maserasi terhadap sampel menggunakan pelarut etanol, dan dilakukan fraksinasi menggunakan pelarut metanol, n-heksan dan kloroform. Aktivitas antimikroba dilakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak kasar dan fraksi metanol dari spons Stylissa carteri aktif menghambat pertumbuhan mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans.  Kata Kunci : Stylissa carteri, antimikroba, Staphylococcus aureus, Escherichia coli, Candida albicans.

scholarly journals Green synthesis of silver nanoparticles using aqueous extract of Citrus sinensis peels and evaluation of their antibacterial efficacy

2021 ◽  
Vol 10 (1) ◽  
pp. 851-859
Author(s):  
Lebogang Mogole ◽  
Wesley Omwoyo ◽  
Elvera Viljoen ◽  
Makwena Moloto
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Citrus Sinensis ◽  
Antimicrobial Activities ◽  
Antibacterial Efficacy ◽  
Gram Positive ◽  
Gram Negative ◽  
Average Size ◽  
Passivating Agent

Abstract The resistance of microorganisms towards antibiotics remains a big challenge in medicine. Silver nanoparticles (AgNPs) received attention recently for their characteristic nanosized features and their ability to display antimicrobial activities. This work reports the synthesis of AgNPs using the Citrus sinensis peels extract in their aqueous, mild, and less hazardous conditions. The effect of concentration variation (1%, 2%, and 3%) of the plant extracts on the size and shape of the AgNPs was investigated. The antimicrobial activities were tested against gram-positive Staphylococcus aureus and gram-negative Klebsiella pneumoniae. Absorption spectra confirmed the synthesis by the surface Plasmon resonance peaks in the range 400–450 nm for all the AgNPs. FTIR spectra confirmed that Citrus sinensis peels extract acted as both reducing and surface passivating agent for the synthesized AgNPs. TEM revealed spherical AgNPs with average size of 12 nm for 3% concentration as compared to the agglomeration at 1% and 2%. All the AgNPs synthesized using Citrus sinensis peels extracts (1%, 2%, and 3%) exhibited antimicrobial activity against both gram-positive and negative bacteria. These results indicated a simple, fast, and inexpensive synthesis of silver nanoparticles using the Citrus sinensis peels extract that has promising antibacterial activity.

scholarly journals Complete Genome Sequence of the Methicillin-Resistant Staphylococcus aureus Strain SQL1/USA300, Used for Testing the Antimicrobial Properties of Clay Phyllosilicates and Customized Aluminosilicates

2021 ◽  
Vol 10 (45) ◽  
Author(s):  
Enrique G. Medrano ◽  
Shelley E. Haydel
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Properties ◽  
Aureus Strain ◽  
Positive Bacterium ◽  
Methicillin Resistant ◽  
Content Type ◽  
Factors Associated ◽  
Critical Knowledge

Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive bacterium that causes community-acquired and health care-acquired infections. We previously demonstrated that clay phyllosilicates and customized aluminosilicates display antimicrobial activity against the MRSA strain SQL1. The SQL1 annotated genome reveals a USA300 lineage and contributes critical knowledge of the MRSA virulence factors associated with tissue infection.

scholarly journals Oxidative Stress and Antimicrobial Activity of Chromium(III) and Ruthenium(II) Complexes onStaphylococcus aureusandEscherichia coli

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina L. Páez ◽  
Claudia M. Bazán ◽  
María E. Bongiovanni ◽  
Judith Toneatto ◽  
Inés Albesa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Diimine Ligands

The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat previously easily treatable infections. The main aim of this work was to establish the potential of the syntheticα-diimine chromium(III) and ruthenium(II) complexes (where theα-diimine ligands are bpy = 2,2-bipyridine, phen = 1,10-phenanthroline, and dppz = dipyrido[3,2-a:2′,3′-c]-phenazine) like [Cr(phen)3]3+, [Cr(phen)2(dppz)]3+, [Ru(phen)3]2+, and [Ru(bpy)3]2+as antibacterial agents by generating oxidative stress. The [Cr(phen)3]3+and [Cr(phen)2(dppz)]3+complexes showed activity against Gram positive and Gram negative bacteria with minimum inhibitory concentrations (MICs) ranging from 0.125 μg/mL to 1 μg/mL, while [Ru(phen)3]2+and [Ru(bpy)3]2+do not exhibit antimicrobial activity against the two bacterial genera studied at the concentration range used. When ciprofloxacin was combined with [Cr(phen)3]3+for the inhibition ofStaphylococcus aureusandEscherichia coli, an important synergistic effect was observed, FIC 0.066 forS. aureusand FIC 0.064 forE. coli. The work described here shows that chromium(III) complexes are bactericidal forS. aureusandE. coli. Our results indicate thatα-diimine chromium(III) complexes may be interesting to open new paths for metallodrug chemotherapy against different bacterial genera since some of these complexes have been found to exhibit remarkable antibacterial activities.

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