Evaluation of the genotoxic properties of nickel oxide nanoparticles in vitro and in vivo

Chemically nickel oxide nanoparticles (NiONPs) involve the synthesis of toxic products, which restrict their biological applications. Hence, we developed a simple, eco-friendly, and cost-efficient green chemistry method for the fabrication of NiONPs using fresh leaf broth of Rhamnus triquetra (RT). The RT leaves broth was used as a strong reducing, capping, and stabilizing agent in the formation of RT-NiONPs. The color change in solution from brown to greenish black suggests the fabrication of RT-NiONPs which was further confirmed by absorption band at 333 nm. The synthesis and different physicochemical properties of RT-NiONPs were investigated using different analytical techniques such as UV-Vis (ultraviolet−visible spectroscopy), XRD (X-ray powder diffraction), FT-IR (Fourier-transform infrared spectroscopy), SEM (scanning electron microscopy), TEM (transmission electron microscopy), EDS (energy-dispersive X-ray spectroscopy), DLS (dynamic light scattering) and Raman. Further, RT-NiONPs were subjected to different in vitro biological activities and revealed distinctive biosafe and biocompatibility potentials using erythrocytes and macrophages. RT-NiONPs exhibited potential anticancer activity against liver cancer cell lines HUH7 (IC50: 11.3 µg/mL) and HepG2 (IC50: 20.73 µg/mL). Cytotoxicity potential was confirmed using Leishmanial parasites promastigotes (IC50: 27.32 µg/mL) and amastigotes (IC50: 37.4 µg/mL). RT-NiONPs are capable of rendering significant antimicrobial efficacy using various bacterial and fungal strains. NiONPs determined potent radical scavenging and moderate enzyme inhibition potencies. Overall, this study suggested that RT-NiONPs can be an attractive and eco-friendly candidate. In conclusion, current study showed potential in vitro biological activities and further necessitate different in vivo studies in various animal models to develop leads for new drugs to treat several chronic diseases.

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Antimycobacterial, Antioxidant and Cytotoxicity Activities of Mesoporous Nickel Oxide Nanoparticles for Healthcare

Coatings ◽

10.3390/coatings10121242 ◽

2020 ◽

Vol 10 (12) ◽

pp. 1242

Author(s):

Jivan R. Kote ◽

Ambadas S. Kadam ◽

Mohd Ubaidullah ◽

Abdullah M. Al-Enizi ◽

Mohammed A. Al-Abdrabalnabi ◽

...

Keyword(s):

Electron Microscopy ◽

Nickel Oxide ◽

Longitudinal Optical ◽

Oxide Nanoparticles ◽

Potential Candidate ◽

Nickel Oxide Nanoparticles ◽

Silar Method ◽

Hemolysis Assay ◽

Nio Nps

Nanobiotechnology has offered great attention in drug delivery and the development of various medicines used to treat microorganism infections. The present investigation deals with antimycobacterial activity, in-vitro hemolysis assay, and antioxidant activity of nickel oxide nanoparticles (NiO NPs). NiO NPs, with controlled size and shape, prepared by a simple and inexpensive successive ionic layer adsorption and reaction (SILAR) method was scanned using field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HR-TEM) digital images for surface morphology confirmation. Spherical irregular island-type NPs of about 24 nm diameter are obtained. The X-ray diffraction pattern demonstrates the synthesis of polycrystalline and cubic in phase NiO NPs. The Raman spectrum has revealed the presence of two vibration bands cantered at 550 and 1095 cm−1 for one photon longitudinal optical, and two longitudinal optical modes, respectively. The as-prepared NiO NPs endow 10 µg/mL against Mycobacterium tuberculosis (M. tuberculosis, MTCC-300) and 10 µg/mL against Mycobacterium phlei (M. phlei, MTCC-1723) inhibitory concentrations. The hemolytic activity of NiO NPs has also been explored. The antioxidant result demonstrates 63.44% for NiO NPs over 88.23% for standard, i.e., di(phenyl)-(2, 4, 6-trinitrophenyl) viminoazaniun antioxidant. Taken together, NiO NPs act as a potential candidate against mycobacteria.

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In vivo genotoxicity assessment of nickel oxide nanoparticles in the model plant Allium cepa L

The Nucleus ◽

10.1007/s13237-021-00377-w ◽

2021 ◽

Author(s):

Indrani Manna ◽

Sovan Mishra ◽

Maumita Bandyopadhyay

Keyword(s):

Nickel Oxide ◽

Allium Cepa ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles ◽

Model Plant ◽

Allium Cepa L

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Toxic Effects of Low-Level Long-Term Inhalation Exposures of Rats to Nickel Oxide Nanoparticles

International Journal of Molecular Sciences ◽

10.3390/ijms20071778 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1778 ◽

Cited By ~ 9

Author(s):

Sutunkova ◽

Solovyeva ◽

Minigalieva ◽

Gurvich ◽

Valamina ◽

...

Keyword(s):

Nickel Oxide ◽

Exposure Period ◽

Lavage Fluid ◽

Genotoxic Effect ◽

Oxide Nanoparticles ◽

Toxic Exposure ◽

Olfactory Pathway ◽

Nickel Oxide Nanoparticles ◽

Nio Nps

Rats were exposed to nickel oxide nanoparticles (NiO-NP) inhalation at 0.23 ± 0.01 mg/m3 for 4 h a day 5 times a week for up to 10 months. The rat organism responded to this impact with changes in cytological and some biochemical characteristics of the bronchoalveolar lavage fluid along with a paradoxically little pronounced pulmonary pathology associated with a rather low chronic retention of nanoparticles in the lungs. There were various manifestations of systemic toxicity, including damage to the liver and kidneys; a likely allergic syndrome as indicated by some cytological signs; transient stimulation of erythropoiesis; and penetration of nickel into the brain from the nasal mucous membrane along the olfactory pathway. Against a picture of mild to moderate chronic toxicity of nickel, its in vivo genotoxic effect assessed by the degree of DNA fragmentation in nucleated blood cells (the RAPD test) was pronounced, tending to increasing with the length of the exposure period. When rats were given orally, in parallel with the toxic exposure, a set of innocuous substances with differing mechanisms of expected bioprotective action, the genotoxic effect of NiO-NPs was found to be substantially attenuated.

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In vitro Cytotoxicity, MMP and ROS activity of green synthesized nickel oxide nanoparticles using extract of Terminalia chebula against MCF-7 cells

Materials Letters ◽

10.1016/j.matlet.2018.09.075 ◽

2019 ◽

Vol 234 ◽

pp. 129-133 ◽

Cited By ~ 8

Author(s):

Fatima Ibraheem ◽

Muhammad Hammad Aziz ◽

Mahvish Fatima ◽

Fozia Shaheen ◽

Syed Mansoor Ali ◽

...

Keyword(s):

Nickel Oxide ◽

In Vitro Cytotoxicity ◽

Oxide Nanoparticles ◽

Terminalia Chebula ◽

Nickel Oxide Nanoparticles ◽

Mcf 7

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Green Synthesis of Nickel Oxide Nanoparticles from Berberis balochistanica Stem for Investigating Bioactivities

Molecules ◽

10.3390/molecules26061548 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1548

Author(s):

Siraj Uddin ◽

Luqman Bin Safdar ◽

Saeed Anwar ◽

Javed Iqbal ◽

Sabiha Laila ◽

...

Keyword(s):

Nickel Oxide ◽

Green Synthesis ◽

Antioxidant Activities ◽

Germination Rate ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

Nickel Oxide Nanoparticles ◽

Total Antioxidant ◽

Elemental Nature

Green synthesis of nanomaterials is advancing due to its ease of synthesis, inexpensiveness, nontoxicity and renewability. In the present study, an eco-friendly biogenic method was developed for the green synthesis of nickel oxide nanoparticles (NiONPs) using phytochemically rich Berberis balochistanica stem (BBS) extract. The BBS extract was rich in phenolics, flavonoids and berberine. These phytochemicals successfully reduced and stabilised the NiNO3 (green) into NiONPs (greenish-gray). BBS-NiONPs were confirmed by using UV-visible spectroscopy (peak at 305 nm), X-ray diffraction (size of 31.44 nm), Fourier transform infrared spectroscopy (identified -OH group and Ni-O formation), energy dispersive spectroscopy (showed specified elemental nature) and scanning electron microscopy (showed rhombohedral agglomerated shape). BBS-NiONPs were exposed to multiple in vitro bioactivities to ascertain their beneficial biological applications. They exhibited strong antioxidant activities: total antioxidant capacity (64.77%) and 2, 2-diphenyl-1-picrylhydrazyl (71.48%); and cytotoxic potential: Brine shrimp cytotoxicity assay with IC50 (10.40 µg/mL). BBS-NiONPs restricted the bacterial and fungal pathogenic growths at 1000, 500 and 100 µg/mL. Additionally, BBS-NiONPs showed stimulatory efficacy by enhancing seed germination rate and seedling growth at 31.25 and 62.5 µg/mL. In aggregate, BBS extract has a potent antioxidant activity which makes the green biosynthesis of NiONPs easy, economical and safe. The biochemical potential of BBS-NiONPs can be useful in various biomedical and agricultural fields.

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TOXIC EFFECTS ASSESSMENT OF NICKEL OXIDE NANOPARTICLES BY INHALATION

ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT ◽

10.35627/2219-5238/2018-309-12-24-30 ◽

2018 ◽

pp. 24-29 ◽

Cited By ~ 2

Author(s):

B.A. Katsnelson ◽

M.P. Sutunkova ◽

L.I. Privalova ◽

S.N. Solovjeva ◽

V.B. Gurvich ◽

...

Keyword(s):

Nickel Oxide ◽

Redox Balance ◽

Systemic Toxicity ◽

Laboratory Animals ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles ◽

White Rats ◽

Liver And Kidney ◽

Stimulation Of Erythropoiesis ◽

Nio Nps

The article presents in an experiment obtained principal results based on repeated low-level inhalation exposures of laboratory animals (white rats, outbred) to nickel oxide nanoparticles with a diameter of (23 ± 5) nm, 4 hours a day, 5 times a week for up to 10 months in a «nose only» installation. It was shown that non-specific body reactions to the action of NiO NPs include: diverse manifestations of systemic toxicity with a particularly pronounced influence on liver and kidney function, redox balance, damage to some areas of brain tissue, associated with proven movement of the nanoparticles themselves from the nasal mucosa along the olfactory tract; some cytological signs of probable development for allergic syndrome; paradoxically low severity of pulmonary pathology by pneumoconiotic type explained by a small chronic delay of nanoparticles in the lungs; the genotoxic effect of the organismal level, even at those low levels of chronic exposure, at which systemic toxicity is rather poorly. Along with that, NiO NPs also induce phase-stimulation of erythropoiesis, which is relatively specific for the toxic nickel effects.

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