Phytogenic Synthesis of Nickel Oxide Nanoparticles (NiO) Using Fresh Leaves Extract of Rhamnus triquetra (Wall.) and Investigation of Its Multiple In Vitro Biological Potentials

Chemically nickel oxide nanoparticles (NiONPs) involve the synthesis of toxic products, which restrict their biological applications. Hence, we developed a simple, eco-friendly, and cost-efficient green chemistry method for the fabrication of NiONPs using fresh leaf broth of Rhamnus triquetra (RT). The RT leaves broth was used as a strong reducing, capping, and stabilizing agent in the formation of RT-NiONPs. The color change in solution from brown to greenish black suggests the fabrication of RT-NiONPs which was further confirmed by absorption band at 333 nm. The synthesis and different physicochemical properties of RT-NiONPs were investigated using different analytical techniques such as UV-Vis (ultraviolet−visible spectroscopy), XRD (X-ray powder diffraction), FT-IR (Fourier-transform infrared spectroscopy), SEM (scanning electron microscopy), TEM (transmission electron microscopy), EDS (energy-dispersive X-ray spectroscopy), DLS (dynamic light scattering) and Raman. Further, RT-NiONPs were subjected to different in vitro biological activities and revealed distinctive biosafe and biocompatibility potentials using erythrocytes and macrophages. RT-NiONPs exhibited potential anticancer activity against liver cancer cell lines HUH7 (IC50: 11.3 µg/mL) and HepG2 (IC50: 20.73 µg/mL). Cytotoxicity potential was confirmed using Leishmanial parasites promastigotes (IC50: 27.32 µg/mL) and amastigotes (IC50: 37.4 µg/mL). RT-NiONPs are capable of rendering significant antimicrobial efficacy using various bacterial and fungal strains. NiONPs determined potent radical scavenging and moderate enzyme inhibition potencies. Overall, this study suggested that RT-NiONPs can be an attractive and eco-friendly candidate. In conclusion, current study showed potential in vitro biological activities and further necessitate different in vivo studies in various animal models to develop leads for new drugs to treat several chronic diseases.

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Antimycobacterial, Antioxidant and Cytotoxicity Activities of Mesoporous Nickel Oxide Nanoparticles for Healthcare

Coatings ◽

10.3390/coatings10121242 ◽

2020 ◽

Vol 10 (12) ◽

pp. 1242

Author(s):

Jivan R. Kote ◽

Ambadas S. Kadam ◽

Mohd Ubaidullah ◽

Abdullah M. Al-Enizi ◽

Mohammed A. Al-Abdrabalnabi ◽

...

Keyword(s):

Electron Microscopy ◽

Nickel Oxide ◽

Longitudinal Optical ◽

Oxide Nanoparticles ◽

Potential Candidate ◽

Nickel Oxide Nanoparticles ◽

Silar Method ◽

Hemolysis Assay ◽

Nio Nps

Nanobiotechnology has offered great attention in drug delivery and the development of various medicines used to treat microorganism infections. The present investigation deals with antimycobacterial activity, in-vitro hemolysis assay, and antioxidant activity of nickel oxide nanoparticles (NiO NPs). NiO NPs, with controlled size and shape, prepared by a simple and inexpensive successive ionic layer adsorption and reaction (SILAR) method was scanned using field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HR-TEM) digital images for surface morphology confirmation. Spherical irregular island-type NPs of about 24 nm diameter are obtained. The X-ray diffraction pattern demonstrates the synthesis of polycrystalline and cubic in phase NiO NPs. The Raman spectrum has revealed the presence of two vibration bands cantered at 550 and 1095 cm−1 for one photon longitudinal optical, and two longitudinal optical modes, respectively. The as-prepared NiO NPs endow 10 µg/mL against Mycobacterium tuberculosis (M. tuberculosis, MTCC-300) and 10 µg/mL against Mycobacterium phlei (M. phlei, MTCC-1723) inhibitory concentrations. The hemolytic activity of NiO NPs has also been explored. The antioxidant result demonstrates 63.44% for NiO NPs over 88.23% for standard, i.e., di(phenyl)-(2, 4, 6-trinitrophenyl) viminoazaniun antioxidant. Taken together, NiO NPs act as a potential candidate against mycobacteria.

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Physicochemical, Photocatalytic, Antibacterial, and Antioxidant Screening of Bergenia Ciliata Mediated Nickel Oxide Nanoparticles

Crystals ◽

10.3390/cryst11091137 ◽

2021 ◽

Vol 11 (9) ◽

pp. 1137

Author(s):

Fazal Ur Rehman ◽

Rashid Mahmood ◽

Manel Ben Ali ◽

Amor Hedfi ◽

Amine Mezni ◽

...

Keyword(s):

Electron Microscopy ◽

Nickel Oxide ◽

Average Particle Size ◽

Diffusion Method ◽

Resistant Bacteria ◽

Oxide Nanoparticles ◽

Average Particle ◽

Nickel Oxide Nanoparticles ◽

X Ray ◽

Nio Nps

This study was planned to synthesize a multifunctional nanomaterial that can effectively encounter the organic pollutants, multidrug-resistant bacteria and reactive free radicals. The Bergenia ciliate (B. ciliate) leaves extract was used as a reducing and capping agent for the synthesis of nickel oxide nanoparticles (NiO NP). The physicochemical properties were studied through X-ray diffractometre (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible and Fourier transform infrared (FTIR) spectroscopies. The highly crystalline monoclinic NiO NPs were synthesized with crystallite size of 27.45 nm. The average particle size derived from TEM micrograph was 49.35 nm whereas the calculated band gap for NiO NPs was 3.78 eV. The photocatalytic study shows that 92.17% of the rhodamine 6G (Rh-6G) was efficiently degraded in the presence of NiO NPs. The agar well diffusion method was applied to examine the antibacterial activity of NiO NPs and the activity was found higher against Gram-negative bacteria (GNB) as compared to Gram-positive bacteria (GPB). The ABTS free radical scavenging activity was also performed, however, the activity was found less than the standard.

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Phytosynthetic Fabrication of Lanthanum Ion-Doped Nickel Oxide Nanoparticles Using Sesbania grandiflora Leaf Extract and Their Anti-Microbial Properties

Crystals ◽

10.3390/cryst11020124 ◽

2021 ◽

Vol 11 (2) ◽

pp. 124

Author(s):

Srihasam Saiganesh ◽

Thyagarajan Krishnan ◽

Golla Narasimha ◽

Hesham S. Almoallim ◽

Sulaiman Ali Alhari ◽

...

Keyword(s):

Electron Microscopy ◽

Optical Properties ◽

Rare Earth ◽

Nickel Oxide ◽

Photoelectron Spectroscopy ◽

Metal Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Nio Nanoparticles ◽

X Ray ◽

Sesbania Grandiflora

Over the past few years, the photogenic fabrication of metal oxide nanoparticles has attracted considerable attention, owing to the simple, eco-friendly, and non-toxic procedure. Herein, we fabricated NiO nanoparticles and altered their optical properties by doping with a rare earth element (lanthanum) using Sesbania grandiflora broth for antibacterial applications. The doping of lanthanum with NiO was systematically studied. The optical properties of the prepared nanomaterials were investigated through UV-Vis diffuse reflectance spectra (UV-DRS) analysis, and their structures were studied using X-ray diffraction analysis. The morphological features of the prepared nanomaterials were examined by scanning electron microscopy and transmission electron microscopy, their elemental structure was analyzed by energy-dispersive X-ray spectral analysis, and their oxidation states were analyzed by X-ray photoelectron spectroscopy. Furthermore, the antibacterial action of NiO and La-doped NiO nanoparticles was studied by the zone of inhibition method for Gram-negative and Gram-positive bacterial strains such as Escherichia coli and Bacillus sublitis. It was evident from the obtained results that the optimized compound NiOLa-04 performed better than the other prepared compounds. To the best of our knowledge, this is the first report on the phytosynthetic fabrication of rare-earth ion Lanthanum (La3+)-doped Nickel Oxide (NiO) nanoparticles and their anti-microbial studies.

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Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽

10.3390/molecules26071919 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1919

Author(s):

Elsayim Rasha ◽

AlOthman Monerah ◽

Alkhulaifi Manal ◽

Ali Rehab ◽

Doud Mohammed ◽

...

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Klebsiella Pneumoniae ◽

Zinc Oxide Nanoparticles ◽

Acacia Nilotica ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

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Spectroscopic, Structural, and Morphology of Nickel Oxide Nanoparticles Prepared Using Physalis angulata Leaf Extract

Materials Science Forum ◽

10.4028/www.scientific.net/msf.917.167 ◽

2018 ◽

Vol 917 ◽

pp. 167-171 ◽

Cited By ~ 9

Author(s):

Najmawati Sulaiman ◽

Yoki Yulizar

Keyword(s):

Electron Microscopy ◽

Particle Size ◽

Nickel Oxide ◽

Band Gap ◽

Leaf Extract ◽

Optical Band Gap ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles ◽

Microscopic Studies ◽

Nio Nps

Green synthesis of nickel oxide nanoparticles (NiO NPs) usingPhysalisangulataleaf extract (PALE) as weak base sources and stabilizing agents has been reported. Chemical bonding and vibration spectroscopy, crystallographic structure, optical band gap, particle size and microscopic studies of NiO NPs were also investigated. Ni-O vibration modes of NiO NPs were analyzed by FTIR and Raman instrument at ~400 and ~900 cm-1wavenumber. XRD pattern of NiO NPs confirmed cubic crystal structure with space groupFm-3m.Optical band gap of NiO NPs determined by using Tauc plot method was about 3.42 eV. Particle size analyzer showed size distribution of NiO NPs was 64.13 nm which confirm NiO formed in nanoscale. Electron microscopic studies of NiO NPs were observed by using scanning electron microscopy and transmission electron microscopy.

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Evaluation of the genotoxic properties of nickel oxide nanoparticles in vitro and in vivo

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2018.06.003 ◽

2018 ◽

Vol 836 ◽

pp. 47-53 ◽

Cited By ~ 8

Author(s):

Raíne F. De Carli ◽

Débora dos S. Chaves ◽

Tatiane R. Cardozo ◽

Ana Paula de Souza ◽

Allan Seeber ◽

...

Keyword(s):

Nickel Oxide ◽

Oxide Nanoparticles ◽

Nickel Oxide Nanoparticles

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In Vitro Evaluation of Cytotoxic Effects of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y)

Qom Univ Med Sci J ◽

10.52547/qums.14.11.20 ◽

2021 ◽

Vol 14 (11) ◽

pp. 20-29

Author(s):

Mozhdeh Hajimohammadjafar tehrania ◽

Mahsa Ale-Ebrahim ◽

Mojtaba Falahati ◽

Shahram Zarabiyan ◽

◽

...

Keyword(s):

Nickel Oxide ◽

Neuroblastoma Cell ◽

Neuroblastoma Cell Line ◽

Oxide Nanoparticles ◽

Human Neuroblastoma Cell ◽

Nickel Oxide Nanoparticles ◽

Human Neuroblastoma ◽

Cytotoxic Effects ◽

Human Neuroblastoma Cell Line

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Plant Extract Mediated Synthesis of Nickel Oxide Nanoparticles

Materials International ◽

10.33263/materials22.205209 ◽

2020 ◽

Vol 2 (2) ◽

pp. 205-209

Keyword(s):

Fourier Transform ◽

Nickel Oxide ◽

Fourier Transform Infrared ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

Leaf Extracts ◽

Nickel Oxide Nanoparticles ◽

X Ray ◽

Chloride Hexahydrate ◽

Scanning Electron

The present study reports the successful synthesis of nickel oxide nanoparticles using Vernonia amygdalina plant leaf extracts as a chelating agent and nickel (II) chloride hexahydrate (NiCl2•6H2O) as precursor. The synthesized powder was gray black in color and annealed at 500 °C for 2 hours to obtain nickel oxide nanoparticles. Characterization techniques such as powder X-ray diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy were used to study the structure and morphology of the nanoparticles. Powder X-ray diffraction patterns revealed that nickel oxide nanoparticles with an average crystallite size of 17.86nm were synthesized. Scanning electron microscope images show that the nanoparticles have octahedral structure. Fourier transform infrared spectrophotometer analysis revealed that the strongest bond at 1094.8cm-1 corresponds to stretching vibration mode of Ni-O nanoparticles.

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Effect of Engineered Nickel Oxide Nanoparticles on Antioxidant Enzymes in Freshwater Fish, Labeo rohita

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2021.044 ◽

2021 ◽

Vol 41 (03) ◽

pp. 424-428

Author(s):

Sana Aziz

Keyword(s):

Superoxide Dismutase ◽

Nickel Oxide ◽

Labeo Rohita ◽

Aquatic Organisms ◽

Lethal Concentration ◽

Oxide Nanoparticles ◽

Human Beings ◽

Nickel Oxide Nanoparticles ◽

X Ray ◽

Nio Nps

Nickel oxide nanoparticles (NiO-NPs) are abundantly utilized on a large scale in different applications due to positive attributes and cause environmental pollution that may affect not only aquatic organisms but also the human beings. The co-precipitation method was used to synthesize NiO-NPs and particles were characterized through Fourier-transform infrared (FTIR) analysis, Scanning electron microscope (SEM) and X-ray diffraction (XRD). FTIR and SEM confirmed the attached functional group and crystal structure of synthesized nanoparticles, respectively. From XRD pattern, average particle size, X-ray density, lattice parameters (a, b and c) and volume of unit cell of nickel oxide nanoparticles were found to be 53.44 nm, 6.65 g/cm3, (a =b=c=4.56 Å) and 94.81 Å3, respectively. During this study, the acute toxicity of NiO-NPs was determined by using fish, Labeo rohita. The mean 96-h LC50 and lethal concentration were measured as 418.26 and 634.94 mg/L, respectively. The activity of catalase and superoxide dismutase was determined in fish gills and liver after chronic exposure to sub-lethal concentration of NiO-NPs for 90 days and sampling was done in 15, 30, 45, 60, 75 and 90 days. Significant time dependent variations in the activity of catalase and superoxide dismutase were determined in tissues of the gills and liver than control group during studied time interval. The overall results indicated that induced toxicity of NiO-NPs in aquatic organisms may be due to release of Ni ions from NiO-NPs and NPs induce toxicity in cells through oxidative stress under long term exposure.

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