The impact of childhood maltreatment on redox state: Relationship with oxidative damage and antioxidant defenses in adolescents with no psychiatric disorder

Growing evidence indicates that the regulation of intracellular reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels is essential for maintaining normal β-cell glucose responsiveness. While long-term exposure to high glucose induces oxidative stress in β cells, conflicting results have been published regarding the impact of ROS on acute glucose exposure and their role in glucose stimulated insulin secretion (GSIS). Although β cells are considered to be particularly vulnerable to oxidative damage, as they express relatively low levels of some peroxide-metabolizing enzymes such as catalase and glutathione (GSH) peroxidase, other less known GSH-based antioxidant systems are expressed in β cells at higher levels. Herein, we discuss the key mechanisms of ROS/RNS production and their physiological function in pancreatic β cells. We also hypothesize that specific interactions between RNS and ROS may be the cause of the vulnerability of pancreatic β cells to oxidative damage. In addition, using a hypothetical metabolic model based on the data available in the literature, we emphasize the importance of amino acid availability for GSH synthesis and for the maintenance of β-cell function and viability during periods of metabolic disturbance before the clinical onset of diabetes.

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Impact of Plasma Oxidative Stress Markers on Post-race Recovery in Ultramarathon Runners: A Sex and Age Perspective Overview

Antioxidants ◽

10.3390/antiox10030355 ◽

2021 ◽

Vol 10 (3) ◽

pp. 355

Author(s):

Carlos Guerrero ◽

Eladio Collado-Boira ◽

Ignacio Martinez-Navarro ◽

Barbara Hernando ◽

Carlos Hernando ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Oxidative Damage ◽

Antioxidant Defenses ◽

Lipid Peroxidation Product ◽

Finish Line ◽

Stress Markers ◽

Markers Of Oxidative Stress ◽

The Impact ◽

Plasma Markers

Oxidative stress has been widely studied in association to ultra-endurance sports. Although it is clearly demonstrated the increase in reactive oxygen species and free radicals after these extreme endurance exercises, the effects on the antioxidant defenses and the oxidative damage to macromolecules, remain to be fully clarified. Therefore, the aim of this study was to elucidate the impact of an ultramarathon race on the plasma markers of oxidative stress of 32 runners and their post-race recovery, with especial focused on sex and age effect. For this purpose, the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) activity, as well as the lipid peroxidation product malondialdehyde (MDA) and the carbonyl groups (CG) content were measured before the race, in the finish line and 24 and 48 h after the race. We have reported an increase of the oxidative damage to lipids and proteins (MDA and CG) after the race and 48 h later. Moreover, there was an increase of the GR activity after the race. No changes were observed in runners’ plasma GPx activity throughout the study. Finally, we have observed sex and age differences regarding damage to macromolecules, but no differences were found regarding the antioxidant enzymes measured. Our results suggest that several basal plasma markers of oxidative stress might be related to the extent of muscle damage after an ultraendurance race and also might affect the muscle strength evolution.

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Effect of Bacillus spp. and Brevibacillus sp. on the Photosynthesis and Redox Status of Solanum lycopersicum

Horticulturae ◽

10.3390/horticulturae7020024 ◽

2021 ◽

Vol 7 (2) ◽

pp. 24

Author(s):

Marino Costa-Santos ◽

Nuno Mariz-Ponte ◽

Maria Celeste Dias ◽

Luísa Moura ◽

Guilhermina Marques ◽

...

Keyword(s):

Solanum Lycopersicum ◽

Intercellular Co2 Concentration ◽

Redox Status ◽

Antioxidant Defenses ◽

Photosynthetic Parameters ◽

Plant Growth Promoting Bacteria ◽

Potted Plants ◽

Chlorophylls A And B ◽

Bacillus Spp ◽

The Impact

Plant-growth-promoting bacteria (PGPB) are gaining attention as a sustainable alternative to current agrochemicals. This study evaluated the impact of three Bacillus spp. (5PB1, 1PB1, FV46) and one Brevibacillus sp. (C9F) on the important crop tomato (Solanum lycopersicum) using the model cv. ‘MicroTom’. The effects of these isolates were assessed on (a) seedlings’ growth and vigor, and (b) adult potted plants. In potted plants, several photosynthetic parameters (chlorophylls (a and b), carotenoids and anthocyanins contents, transpiration rate, stomatal conductance, net CO2 photosynthetic rate, and intercellular CO2 concentration, and on chlorophyll fluorescence yields of light- and dark-adapted leaves)), as well as soluble sugars and starch contents, were quantified. Additionally, the effects on redox status were evaluated. While the growth of seedlings was, overall, not influenced by the strains, some effects were observed on adult plants. The Bacillus safensis FV46 stimulated the content of pigments, compared to C9F. Bacillus zhangzhouensis 5PB1 increased starch levels and was positively correlated with some parameters of the photophosphorylation and the gas exchange phases. Interestingly, Bacillus megaterium 1PB1 decreased superoxide (O2−) content, and B. safensis FV46 promoted non-enzymatic antioxidant defenses, increasing total phenol content levels. These results, conducted on a model cultivar, support the theory that these isolates differently act on tomato plant physiology, and that their activity depends on the age of the plant, and may differently influence photosynthesis. It would now be interesting to analyze the influence of these bacteria using commercial cultivars.

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Imaging NAD(H) Redox Alterations in Cryopreserved Alveolar Macrophages from Ozone-Exposed Mice and the Impact of Nutrient Starvation during Long Lag Times

Antioxidants ◽

10.3390/antiox10050767 ◽

2021 ◽

Vol 10 (5) ◽

pp. 767

Author(s):

He N. Xu ◽

Joanna Floros ◽

Lin Z. Li ◽

Shaili Amatya

Keyword(s):

Oxidative Stress ◽

Alveolar Macrophages ◽

Redox State ◽

Redox Status ◽

Nutrient Starvation ◽

Ozone Exposure ◽

Reduced Form ◽

Time Period ◽

Lag Times ◽

The Impact

Employing the optical redox imaging technique, we previously identified a significant redox shift of nicotinamide adenine dinucleotide (NAD and the reduced form NADH) in freshly isolated alveolar macrophages (AM) from ozone-exposed mice. The goal here was twofold: (a) to determine the NAD(H) redox shift in cryopreserved AM isolated from ozone-exposed mice and (b) to investigate whether there is a difference in the redox status between cryopreserved and freshly isolated AM. We found: (i) AM from ozone-exposed mice were in a more oxidized redox state compared to that from filtered air (FA)-exposed mice, consistent with the results obtained from freshly isolated mouse AM; (ii) under FA exposure, there was no significant NAD(H) redox difference between fresh AM that had been placed on ice for 2.5 h and cryopreserved AM; however, under ozone exposure, fresh AM were more oxidized than cryopreserved AM; (iii) via the use of nutrient starvation and replenishment and H2O2-induced oxidative stress of an AM cell line, we showed that this redox difference between cryopreserved and freshly isolated AM is likely the result of the double “hit”, i.e., the ozone-induced oxidative stress plus nutrient starvation that prevented freshly isolated AM from a full recovery after being on ice for a prolonged time period. The cryopreservation technique we developed eliminates/minimizes the effects of oxidative stress and nutrient starvation on cells. This method can be adopted to preserve lung macrophages from animal models or clinical patients for further investigations.

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Are Microplastics Impairing Marine Fish Larviculture?—Preliminary Results with Argyrosomus regius

Water ◽

10.3390/w13010104 ◽

2021 ◽

Vol 13 (1) ◽

pp. 104

Author(s):

Diana Campos ◽

Andreia C. M. Rodrigues ◽

Rui J. M. Rocha ◽

Roberto Martins ◽

Ana Candeias-Mendes ◽

...

Keyword(s):

Oxidative Damage ◽

Fish Larvae ◽

Feeding Activity ◽

Early Ontogeny ◽

Antioxidant Defenses ◽

Nutritional Requirements ◽

Larval Feeding ◽

Argyrosomus Regius ◽

And Performance ◽

Aquaculture Facilities

The presence of small-sized (<300 µm) microplastics (MPs) in aquaculture facilities may threaten finfish hatchery, as their (in)voluntary ingestion by fish larvae may compromise nutritional requirements during early ontogeny, and consequently larval health and performance. Thus, we addressed the short-term effects (7 h) of polyethylene microplastics (0.1, 1.0, 10 mg/L, PE-MPs) in meagre larvae Argyrosomus regius (15 dph) in the presence/absence of food. Larval feeding behavior, oxidative stress status, neurotoxicity, and metabolic requirements were evaluated. Results showed that meagre larvae ingested PE-MPs regardless of their concentration, decreasing in the presence of food (Artemia metanauplii). The presence of PE-MPs compromised larval feeding activity at the highest concentration. Under starvation, exposed larvae activated the antioxidant defenses by increasing the total glutathione levels and inhibiting catalase activity, which seemed efficient to prevent oxidative damage. Such larvae also presented increased energy consumption potentially related to oxidative damage prevention and decreased neurotransmission. Biochemical responses of fed larvae showed a similar trend, except for LPO, which remained unaffected, except at 0.1 mg/PE-MPs/L. Our results suggest that small-sized MPs in finfish hatcheries may compromise larvae nutritional requirements, but at considerably higher levels than those reported in marine environments. Nevertheless, cumulative adverse effects due to lower MPs concentrations may occur.

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A manganese oxide nanozyme prevents the oxidative damage of biomolecules without affecting the endogenous antioxidant system

Nanoscale ◽

10.1039/c8nr09397k ◽

2019 ◽

Vol 11 (9) ◽

pp. 3855-3863 ◽

Cited By ~ 22

Author(s):

Namrata Singh ◽

Mohammed Azharuddin Savanur ◽

Shubhi Srivastava ◽

Patrick D'Silva ◽

Govindasamy Mugesh

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Manganese Oxide ◽

Antioxidant System ◽

Mammalian Cells ◽

Redox State ◽

Stress Conditions ◽

System P ◽

Endogenous Antioxidant ◽

Antioxidant Machinery

Multi-enzyme mimetic Mn3O4 nanoflowers (Mp) modulate the redox state of mammalian cells without altering the cellular antioxidant machinery under oxidative stress conditions.

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Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02209-9 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Nadia Y. A. Alaidaroos ◽

Amr Alraies ◽

Rachel J. Waddington ◽

Alastair J. Sloan ◽

Ryan Moseley

Keyword(s):

Stem Cell ◽

Regenerative Medicine ◽

Oxidative Damage ◽

Dental Pulp ◽

Stem Cell Marker ◽

Significant Heterogeneity ◽

Premature Senescence ◽

Cell Marker ◽

Marker Expression ◽

The Impact

Abstract Background Dental pulp stem cells (DPSCs) are increasingly being advocated as viable cell sources for regenerative medicine-based therapies. However, significant heterogeneity in DPSC expansion and multi-potency capabilities are well-established, attributed to contrasting telomere profiles and susceptibilities to replicative senescence. As DPSCs possess negligible human telomerase (hTERT) expression, we examined whether intrinsic differences in the susceptibilities of DPSC sub-populations to oxidative stress-induced biomolecular damage and premature senescence further contributed to this heterogeneity, via differential enzymic antioxidant capabilities between DPSCs. Methods DPSCs were isolated from human third molars by differential fibronectin adhesion, and positive mesenchymal (CD73/CD90/CD105) and negative hematopoietic (CD45) stem cell marker expression confirmed. Isolated sub-populations were expanded in H2O2 (0–200 μM) and established as high or low proliferative DPSCs, based on population doublings (PDs) and senescence (telomere lengths, SA-β-galactosidase, p53/p16INK4a/p21waf1/hTERT) marker detection. The impact of DPSC expansion on mesenchymal, embryonic, and neural crest marker expression was assessed, as were the susceptibilities of high and low proliferative DPSCs to oxidative DNA and protein damage by immunocytochemistry. Expression profiles for superoxide dismutases (SODs), catalase, and glutathione-related antioxidants were further compared between DPSC sub-populations by qRT-PCR, Western blotting and activity assays. Results High proliferative DPSCs underwent > 80PDs in culture and resisted H2O2−induced senescence (50–76PDs). In contrast, low proliferative sub-populations exhibited accelerated senescence (4–32PDs), even in untreated controls (11-34PDs). While telomere lengths were largely unaffected, certain stem cell marker expression declined with H2O2 treatment and expansion. Elevated senescence susceptibilities in low proliferative DPSC (2–10PDs) were accompanied by increased oxidative damage, absent in high proliferative DPSCs until 45–60PDs. Increased SOD2/glutathione S-transferase ζ1 (GSTZ1) expression and SOD activities were identified in high proliferative DPSCs (10–25PDs), which declined during expansion. Low proliferative DPSCs (2–10PDs) exhibited inferior SOD, catalase and glutathione-related antioxidant expression/activities. Conclusions Significant variations exist in the susceptibilities of DPSC sub-populations to oxidative damage and premature senescence, contributed to by differential SOD2 and GSTZ1 profiles which maintain senescence-resistance/stemness properties in high proliferative DPSCs. Identification of superior antioxidant properties in high proliferative DPSCs enhances our understanding of DPSC biology and senescence, which may be exploited for selective sub-population screening/isolation from dental pulp tissues for regenerative medicine-based applications.

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The mediating role of self-esteem in the relationship between childhood maltreatment and god image among Turkish Muslims

Archive for the Psychology of Religion ◽

10.1177/00846724211047274 ◽

2021 ◽

pp. 008467242110472

Author(s):

Ferdi Kıraç

Keyword(s):

Childhood Maltreatment ◽

Short Form ◽

Self Esteem ◽

God Image ◽

Image Of God ◽

Negative Image ◽

Mediating Role ◽

The Impact ◽

The Relationship

Childhood maltreatment is widespread in predominantly Muslim countries. However, the research investigating the impact of childhood maltreatment on the adult survivors’ religious and spiritual lives has mainly focused on Western Judeo-Christian samples. Considering cross-cultural differences in religious beliefs, in this study, we investigated the relationship between childhood maltreatment and god image, and the mediating role of self-esteem in a sample of Muslim Turkish adults. Eight hundred two participants completed Childhood Trauma Questionnaire–Short Form, God Perception Scale, and Self-Esteem Scale. Findings revealed that all childhood maltreatment subtypes predicted negative image of god and self-esteem mediated some of the negative effects of each maltreatment subtype on god image. The study also found that emotional neglect was the most prominent predictor of negative image of god, followed by emotional abuse. Based on attachment theory, we concluded that the emotional component of childhood maltreatment had more long-lasting adverse consequences in survivors’s relationship with god in Muslim adults.

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The relationship between exclusion from school and mental health: a secondary analysis of the British Child and Adolescent Mental Health Surveys 2004 and 2007

Psychological Medicine ◽

10.1017/s003329171700215x ◽

2017 ◽

Vol 48 (4) ◽

pp. 629-641 ◽

Cited By ~ 12

Author(s):

T. Ford ◽

C. Parker ◽

J. Salim ◽

R. Goodman ◽

S. Logan ◽

...

Keyword(s):

Mental Health ◽

Psychological Distress ◽

Psychiatric Disorder ◽

Health Surveys ◽

Secondary Analysis ◽

Adolescent Mental Health ◽

Child And Adolescent ◽

The Impact ◽

The Relationship

BackgroundChildren with poor mental health often struggle at school. The relationship between childhood psychiatric disorder and exclusion from school has not been frequently studied, but both are associated with poor adult outcomes. We undertook a secondary analysis of the British Child and Adolescent Mental Health Surveys from 2004 and its follow-up in 2007 to explore the relationship between exclusion from school and psychopathology. We predicted poorer mental health among those excluded.MethodPsychopathology was measured using the Strengths and Difficulties Questionnaire, while psychiatric disorder was assessed using the Development and Well-Being Assessment and applying Diagnostic and Statistical Manual of Mental Disorders Fourth Edition (DSM IV) criteria. Exclusion from school and socio-demographic characteristics were reported by parents. Multi-variable regression models were used to examine the impact of individual factors on exclusion from school or psychological distress.ResultsExclusion from school was commoner among boys, secondary school pupils and those living in socio-economically deprived circumstances. Poor general health and learning disability among children and poor parental mental health were also associated with exclusion. There were consistently high levels of psychological distress among those who had experienced exclusion at baseline and follow-up.ConclusionsWe detected a bi-directional association between psychological distress and exclusion. Efforts to identify and support children who struggle with school may therefore prevent both future exclusion and future psychiatric disorder.

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