In vitro photodynamic effect of aluminum tetrasulfophthalocyanines on melanoma skin cancer and healthy normal skin cells

2012 ◽  
Vol 9 (1) ◽  
pp. 32-39 ◽  
Author(s):  
K. Maduray ◽  
B. Odhav ◽  
T. Nyokong
Keyword(s):  
Skin Cancer ◽  
Normal Skin ◽  
Photodynamic Effect ◽  
Skin Cells ◽  
Melanoma Skin

NANO-BASED THERAPY FOR TREATMENT OF SKIN CARCINOMA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 7-17
Author(s):  
Goutam K. Jena ◽  
Chinam N. Patra ◽  
Keyword(s):  
Side Effects ◽  
Skin Cancer ◽  
Drug Carrier ◽  
Normal Skin ◽  
Ultra Violet ◽  
Skin Carcinoma ◽  
Conventional Chemotherapy ◽  
Skin Cells ◽  
Life Threatening ◽  
Current Scenario

Skin carcinoma is a frequently occurring cancer caused due to ultra violet rays of the Sun. It starts from normal skin cells but later on transforms into cells which undergo uncontrolled mitosis. Skin cancer is not as deadly as other cancers and has no metastasis and is not life threatening. Conventional chemotherapy has in general failed to treat skin cancer due to non specific targeting, which is accompanied by several side effects. Novel therapeutic approach based on nanotechnology have emerged as the best alternative for skin cancer treatment. We presented current scenario of nano based particulate drug carrier approaches for effective therapy for skin carcinoma by reducing side effects. This approach also reduces frequency of administration and improves patient compliance. Nanotechnology has emerged as the best alternative for conventional therapy for the effective treatment of skin cancer. Nanoparticles can specifically target skin carcinoma and are able to sustain drug release and reduce side effects to a greater extent.

scholarly journals Melanoma Skin Cancer Detection

2019 ◽  
Vol 9 (1) ◽  
pp. 4073-4076
Keyword(s):  
Image Processing ◽  
Skin Cancer ◽  
Cancer Detection ◽  
Normal Skin ◽  
Cancer Growth ◽  
Melanoma Cancer ◽  
Skin Malignancy ◽  
Image Investigation ◽  
Skin Cancer Detection ◽  
Melanoma Skin

Skin cancer growth is viewed as one of the most Hazardous type of the Cancers found in Humans. Nowadays skin cancer is found in different kinds for example Melanoma, Basal and Squamous cell Carcinoma among which Melanoma is the generally flighty. The detection of Melanoma disease in beginning period can be helpful for cure it. Computer vision can play big role in Portrayal Analysis also it has been examined by many existing frameworks. In this paper, we present a Computer helped strategy for the recognition of Melanoma Skin Cancer utilizing Image Processing instruments. The contribution to the framework is the skin lesion picture and after that by applying novel picture preparing strategies, it investigates it to finish up about the nearness of skin malignancy. The Lesion Image investigation instruments checks for the different Melanoma parameters Like Asymmetry, Border, Color, Diameter,(ABCD) and so on by surface, size and shape examination for picture division and highlight stages. The extricated highlight parameters are utilized to characterize the picture as Normal skin and Melanoma cancer growth injury.

scholarly journals Zeaxanthin-Rich Extract from Superfood Lycium barbarum Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 333
Author(s):  
Diana Cenariu ◽  
Eva Fischer-Fodor ◽  
Adrian Bogdan Țigu ◽  
Andrea Bunea ◽  
Piroska Virág ◽  
...  
Keyword(s):  
Normal Skin ◽  
Fibroblast Cell ◽  
Mapk Signaling ◽  
Cellular Adhesion ◽  
Membrane Expression ◽  
Lycium Barbarum ◽  
Skin Cells ◽  
Extracellular Secretion ◽  
Mitogen Activated Protein

The concern for implementing bioactive nutraceuticals in antioxidant-related therapies is of great importance for skin homeostasis in benign or malignant diseases. In order to elucidate some novel insights of Lycium barbarum (Goji berry) activity on skin cells, the present study focused on its active compound zeaxanthin. By targeting the stemness markers CD44 and CD105, with deep implications in skin oxidative stress mechanisms, we revealed, for the first time, selectivity in zeaxanthin activity. When applied in vitro on BJ human fibroblast cell line versus the A375 malignant melanoma cells, despite the moderate cytotoxicity, the zeaxanthin-rich extracts 1 and 2 were able to downregulate significantly the CD44 and CD105 membrane expression and extracellular secretion in A375, and to upregulate them in BJ cells. At mechanistic level, the present study is the first to demonstrate that the zeaxanthin-rich Goji extracts are able to influence selectively the mitogen-activated protein kinases (MAPK): ERK, JNK and p38 in normal BJ versus tumor-derived A375 skin cells. These results point out towards the applications of zeaxanthin from L. barbarum as a cytoprotective agent in normal skin and raises questions about its use as an antitumor prodrug alone or in combination with standard therapy.

scholarly journals Graviola (Annona muricata) Exerts Anti-Proliferative, Anti-Clonogenic and Pro-Apoptotic Effects in Human Non-Melanoma Skin Cancer UW-BCC1 and A431 Cells In Vitro: Involvement of Hedgehog Signaling

2018 ◽  
Author(s):  
Jean Christopher Chamcheu ◽  
Islam Rady ◽  
Roxane-Cherille N. Chamcheu ◽  
Abu Bakar Siddique ◽  
Melissa B. Bloch ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Hedgehog Signaling ◽  
Annona Muricata ◽  
A431 Cells ◽  
Tropical Fruit ◽  
Non Melanoma Skin Cancer ◽  
Apoptotic Protein ◽  
Gli 1 ◽  
Melanoma Skin

Non-melanoma skin cancers (NMSCs) are the leading cause of skin cancer-related morbidity and mortality. Effective strategies are needed to control NMSC occurrence and progression. Non-toxic, plant-derived extracts have been shown to exert multiple anti-cancer effects. Graviola (Annona muricata), a tropical fruit-bearing plant, has been used in traditional medicine against multiple human diseases including cancer. The current study investigated the effects of graviola leaf and stem extract (GLSE) and its solvent-extracted fractions on two human NMSC cell lines, UW-BCC1 and A431. GLSE was found to: i) dose-dependently suppress UW-BCC1 and A431 cell growth, motility, wound closure, and clonogenicity; ii) induce G0/G1 cell cycle arrest by downregulating cyclin/cdk factors while upregulating cdk inhibitors, and (iv) induce apoptosis as evidenced by cleavage of caspases-3, -8 and PARP. Further, GLSE suppressed levels of activated hedgehog (Hh) pathway components Smo, Gli 1/2, and Shh while inducing SuFu. GLSE also decreased the expression of pro-apoptotic protein Bax while decreasing the expression of the anti-apoptotic protein Bcl-2. We determined that these activities were concentrated in an acetogenin/alkaloid-rich dichloromethane subfraction of GLSE. Our data identify graviola extracts and their constituents as promising sources for new chemopreventive and therapeutic agent(s) to be further developed for the control of NMSCs

scholarly journals Preoperative AI-Driven Fluorescence Diagnosis of Non-Melanoma Skin Cancer

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 72
Author(s):  
Victoriya Andreeva ◽  
Evgeniia Aksamentova ◽  
Andrey Muhachev ◽  
Alexey Solovey ◽  
Igor Litvinov ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Normal Skin ◽  
Invasive Procedure ◽  
Biopsy Material ◽  
Fluorescence Diagnosis ◽  
Non Melanoma Skin Cancer ◽  
Skin Cancers ◽  
Abnormal Cells ◽  
Diagnosis Method ◽  
Melanoma Skin

The diagnosis and treatment of non-melanoma skin cancer remain urgent problems. Histological examination of biopsy material—the gold standard of diagnosis—is an invasive procedure that requires a certain amount of time to perform. The ability to detect abnormal cells using fluorescence spectroscopy (FS) has been shown in many studies. This technique is rapidly expanding due to its safety, relative cost-effectiveness, and efficiency. However, skin lesion FS-based diagnosis is challenging due to a number of single overlapping spectra emitted by fluorescent molecules, making it difficult to distinguish changes in the overall spectrum and the molecular basis for it. We applied deep learning (DL) algorithms to quantitatively assess the ability of FS to differentiate between pathologies and normal skin. A total of 137 patients with various forms of primary and recurrent basal cell carcinoma (BCC) were observed by a multispectral laser-based device with a built-in neural network (NN) “DSL-1”. We measured the fluorescence spectra of suspected non-melanoma skin cancers and compared them with “normal” skin spectra. These spectra were input into DL algorithms to determine whether the skin is normal, pigmented normal, benign, or BCC. The preoperative differential AI-driven fluorescence diagnosis method correctly predicted the BCC lesions. We obtained an average sensitivity of 62% and average specificity of 83% in our experiments. Thus, the presented “DSL-1” diagnostic device can be a viable tool for the real-time diagnosis and guidance of non-melanoma skin cancer resection.

scholarly journals Simultaneous Targeting Tumor Cells and Cancer-Associated Fibroblasts with a Paclitaxel–Hyaluronan Bioconjugate: In Vitro Evaluation in Non-Melanoma Skin Cancer

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 597
Author(s):  
Barbara Bellei ◽  
Silvia Caputo ◽  
Emilia Migliano ◽  
Gianluca Lopez ◽  
Valeria Marcaccini ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Apoptotic Cell ◽  
Wnt Signaling Pathway ◽  
Dermal Fibroblasts ◽  
Carcinoma Cells ◽  
Cancer Associated Fibroblasts ◽  
Innovative Therapy ◽  
Non Melanoma Skin Cancer ◽  
Melanoma Skin

Background: Cancer-associated fibroblasts (CAFs) facilitate many aspects of cancer development by providing a structural framework rich in bioactive compounds. There are emerging studies proposing a combination of conventional anti-cancer therapies directed against neoplastic cells to molecules targeting tumor microenvironments. Methods: The study evaluated the pharmacological properties of the anti-tumor agent paclitaxel conjugated to hyaluronic acid (HA) regarding non-melanoma skin cancer (NMSC) and the surrounding fibroblasts. This molecule, named Oncofid-P20 (Onco-P20), preferentially targets cells expressing high levels of CD44, the natural ligand of HA. Results: Consistent with paclitaxel’s mechanism of action involving interference with the breakdown of microtubules during cell division, highly sensitive carcinoma cells rapidly underwent apoptotic cell death. Interestingly, less sensitive cells, such as dermal fibroblasts, resisted the Onco-P20 treatment and experienced a prolonged growth arrest characterized by morphological change and significant modification of the gene expression profile. Onco-P20-treated fibroblasts exhibited reduced growth factor production, downmodulation of the Wnt signaling pathway, and the acquisition of a marked pro-inflammatory profile. Independently of direct exposure to taxol, in the presence of Onco-P20-treated fibroblasts or in their conditioned medium, carcinoma cells had a reduced proliferation rate. Similar to NHF, fibroblasts isolated from skin cancer lesions or from adjacent tissue acquired anti-neoplastic activity under Onco-P20 treatment. Conclusion: Collectively, our data demonstrate that Onco-P20, exerting both a direct and an NHF-mediated indirect effect on carcinoma cells, is a candidate for an innovative therapy alternative to surgery for the treatment of NMSC.

scholarly journals In vitro toxicity testing of phthalocyanines on different cell lines using a continuous laser source

2010 ◽  
Author(s):  
◽  
Kaminee Maduray
Keyword(s):  
Photodynamic Therapy ◽  
Cell Viability ◽  
Cancer Cells ◽  
Normal Skin ◽  
Light Dose ◽  
Laser Source ◽  
Normal Fibroblast ◽  
Melanoma Cancer ◽  
Skin Cells

Photodynamic therapy is a promising treatment for cancer. It involves the combination of a photosensitizer and light of an appropriate wavelength (laser source) to cause the destruction of cancer cells. Phthalocynanines are second–generation photosensitizers with enhanced photophysical and photochemical properties. In this in vitro study the effect of aluminium (AlTSPc) or zinc (ZnTSPc) tetrasulfophthalocyanines in its inactive and active state (laser induced) on melanoma (skin cancer cells), fibroblast (healthy normal skin cells) and keratinocyte (healthy normal skin cells) cells was evaluated. For each of the cell lines approximately 3 x 104 cells/ml were seeded onto 24-well cell culture plates and allowed to attach overnight, after which cells were treated with different concentrations of AlTSPc or ZnTSPc. The photosensitizers were synthesized at Rhodes University. After 2 hrs, cells were irradiated with a diode laser at a wavelength of 672 nm and a beam diameter of 1 cm. The laser power varied between 20-30 mW and the irradiation time was calculated to deliver a light dose of 4.5 J/cm2. Post-irradiated cells were incubated for 24 hrs before cell viability was measured using the CellTiter-BlueTM Viability Assay. Also, the efficacy of the light dose and laser source used for the killing of approximately 50% of the melanoma cancer cells were investigated. AlTSPc and ZnTSPc decreased cell viability of melanoma cancer cells to approximately 50% with photosensitizer concentrations of 40 μg/ml and 50 μg/ml respectively. These photosensitizer concentrations caused a slight decrease in the percentage cell viability of fibroblast and keratinocyte cells. Results for the dark toxicity assay showed that iii both photosensitizers in the presence of high concentrations (60 μg/ml – 100 μg/ml) showed cytotoxicity effects on melanoma cancer cells in their inactive state. This was not observed in fibroblast and keratinocyte cells treated under the same experimental conditions. The optimal AlTSPc and ZnTSPc concentrations in combination with the light dose of 4.5 J/cm2 was the most efficient in killing the melanoma cancer cells with reduced killing effects on healthy normal fibroblast and keratinocyte cells when compared to other light doses (2.5 J/cm2, 7.5 J/cm2 and 10 J/cm2). The irradiation of cells photosensitized with the optimal photosensitizer concentrations with a femtosecond laser using similar laser parameters to continuous wave laser experiments resulted in a reduction in the cell viability of healthy normal fibroblast and keratinocyte cells compared to melanoma cancer cells. The presence of DNA degradation on agarose gel, morphological changes like blebbing and ultrastructural changes like nucleus condensation indicated that photodynamic therapy treated melanoma cancer cells with the optimal concentrations of AlTSPc and ZnTSPc induced cell death via apoptosis. This concludes that low concentrations of AlTSPc and ZnTSPc activated with an appropriate laser source can be used to induce cell death in melanoma cancer cells. Both AlTSPc and ZnTSPc exhibit the potential to be used as a photosensitizer in photodynamic therapy for the treatment of melanoma cancer with the occurrence of minimal damage to surrounding healthy tissue.

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