Salt and methyl jasmonate aggravate growth inhibition and senescence in Arabidopsis seedlings via the JA signaling pathway

BackgroundGlioma has one of the highest mortality rates of all tumors of the nervous system and commonly used treatments almost always fail to achieve tumor control. Low-dose carbon-ion radiation can effectively target cancer and tumor cells, but the mechanisms of growth inhibition induced by heavy-ion radiation via the PI3K/Akt signaling pathway are unknown, and inhibition by heavy-ion radiation is minor in C6 cells.MethodsCarbon-ion radiation was used to investigate the effects of heavy-ion radiation on C6 cells, and suppression of Akt was performed using perifosine. MTT assays were used to investigate optimal perifosine treatment concentrations. Clone formation assays were used to investigate the growth inhibition effects of carbon-ion radiation and the effects of radiation with Akt inhibition. Lactate dehydrogenase release, superoxide dismutase activity, and malondialdehyde content were assessed to investigate oxidative stress levels. Expression levels of proteins in the PI3K/Akt/p53 signaling pathway were assessed via western blotting.ResultsThe 10% maximum inhibitory concentration of perifosine was 19.95 μM. In clone formation assays there was no significant inhibition of cell growth after treatment with heavy-ion irradiation, whereas perifosine enhanced inhibition. Heavy-ion radiation induced lactate dehydrogenase release, increased the level of malondialdehyde, and reduced superoxide dismutase activity. Akt inhibition promoted these processes. Heavy-ion radiation treatment downregulated Akt expression, and upregulated B-cell lymphoma-2 (Bcl-2) expression. p53 and Bcl-2 expression were significantly upregulated, and Bcl-2-associated X protein (Bax) expression was downregulated. The expression profiles of pAkt, Bcl-2, and Bax were reversed by perifosine treatment. Caspase 3 expression was upregulated in all radiation groups.ConclusionsThe growth inhibition effects of low-dose heavy-ion irradiation were not substantial in C6 cells, and Akt inhibition induced by perifosine enhanced the growth inhibition effects via proliferation inhibition, apoptosis, and oxidative stress. Akt inhibition enhanced the effects of heavy-ion radiation, and the PI3K/Akt/p53 signaling pathway may be a critical component involved in the process.

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Arsenic trioxide-mediated growth inhibition of myeloma cells is associated with an extrinsic or intrinsic signaling pathway through activation of TRAIL or TRAIL receptor 2

Cancer Biology & Therapy ◽

10.4161/cbt.10.11.13669 ◽

2010 ◽

Vol 10 (11) ◽

pp. 1201-1214 ◽

Cited By ~ 14

Author(s):

Xiaosong Wu ◽

Jumei Shi ◽

Yi Wu ◽

Yi Tao ◽

Jun Hou ◽

...

Keyword(s):

Growth Inhibition ◽

Arsenic Trioxide ◽

Signaling Pathway ◽

Trail Receptor ◽

Myeloma Cells

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Functions of Jasmonic Acid in Plant Regulation and Response to Abiotic Stress

International Journal of Molecular Sciences ◽

10.3390/ijms21041446 ◽

2020 ◽

Vol 21 (4) ◽

pp. 1446 ◽

Cited By ~ 9

Author(s):

Jia Wang ◽

Li Song ◽

Xue Gong ◽

Jinfan Xu ◽

Minhui Li

Keyword(s):

Transcription Factors ◽

Abiotic Stress ◽

Jasmonic Acid ◽

Signaling Pathways ◽

Signaling Pathway ◽

Large Scale ◽

Higher Plants ◽

Complex Signal ◽

Ja Signaling ◽

Signal Network

Jasmonic acid (JA) is an endogenous growth-regulating substance, initially identified as a stress-related hormone in higher plants. Similarly, the exogenous application of JA also has a regulatory effect on plants. Abiotic stress often causes large-scale plant damage. In this review, we focus on the JA signaling pathways in response to abiotic stresses, including cold, drought, salinity, heavy metals, and light. On the other hand, JA does not play an independent regulatory role, but works in a complex signal network with other phytohormone signaling pathways. In this review, we will discuss transcription factors and genes involved in the regulation of the JA signaling pathway in response to abiotic stress. In this process, the JAZ-MYC module plays a central role in the JA signaling pathway through integration of regulatory transcription factors and related genes. Simultaneously, JA has synergistic and antagonistic effects with abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and other plant hormones in the process of resisting environmental stress.

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NATURAL COMPOUND METHYL JASMONATE SHOWS PROMISING ANTI-FIBROID EFFECTS IN HUMAN UTERINE FIBROIDS VIA INHIBITION OF EZH2 MEDIATED WNT/Β-CATENIN SIGNALING PATHWAY ACTIVATION

Fertility and Sterility ◽

10.1016/j.fertnstert.2020.08.1012 ◽

2020 ◽

Vol 114 (3) ◽

pp. e338

Author(s):

Mohamed Ali ◽

Ayman Al-Hendy ◽

Qiwei Yang

Keyword(s):

Methyl Jasmonate ◽

Signaling Pathway ◽

Natural Compound ◽

Uterine Fibroids ◽

Pathway Activation

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Underexpressed CNDP2 Participates in Gastric Cancer Growth Inhibition through Activating the MAPK Signaling Pathway

Molecular Medicine ◽

10.2119/molmed.2013.00102 ◽

2013 ◽

Vol 20 (1) ◽

pp. 17-28 ◽

Cited By ~ 11

Author(s):

Zhenwei Zhang ◽

Lei Miao ◽

Xiaoming Xin ◽

Jianpeng Zhang ◽

Shengsheng Yang ◽

...

Keyword(s):

Gastric Cancer ◽

Growth Inhibition ◽

Signaling Pathway ◽

Mapk Signaling ◽

Mapk Signaling Pathway ◽

Cancer Growth

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Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

International Journal of Molecular Sciences ◽

10.3390/ijms20122917 ◽

2019 ◽

Vol 20 (12) ◽

pp. 2917 ◽

Cited By ~ 5

Author(s):

Yuya Uji ◽

Keita Kashihara ◽

Haruna Kiyama ◽

Susumu Mochizuki ◽

Kazuya Akimitsu ◽

...

Keyword(s):

Grain Size ◽

Jasmonic Acid ◽

Signaling Pathway ◽

Mitogen Activated Protein Kinase ◽

Rice Plants ◽

Rice Bacterial Blight ◽

Expression Of Genes ◽

Mitogen Activated Protein ◽

Stable Growth ◽

Ja Signaling

Jasmonic acid (JA) is a plant hormone that plays an important role in the defense response and stable growth of rice. In this study, we investigated the role of the JA-responsive valine-glutamine (VQ)-motif-containing protein OsVQ13 in JA signaling in rice. OsVQ13 was primarily located in the nucleus and cytoplasm. The transgenic rice plants overexpressing OsVQ13 exhibited a JA-hypersensitive phenotype and increased JA-induced resistance to Xanthomonas oryzae pv. oryzae (Xoo), which is the bacteria that causes rice bacterial blight, one of the most serious diseases in rice. Furthermore, we identified a mitogen-activated protein kinase, OsMPK6, as an OsVQ13-associating protein. The expression of genes regulated by OsWRKY45, an important WRKY-type transcription factor for Xoo resistance that is known to be regulated by OsMPK6, was upregulated in OsVQ13-overexpressing rice plants. The grain size of OsVQ13-overexpressing rice plants was also larger than that of the wild type. These results indicated that OsVQ13 positively regulated JA signaling by activating the OsMPK6–OsWRKY45 signaling pathway in rice.

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Arsenic Trioxide-Mediated Growth Inhibition of Myeloma Cells Is Associated with An Extrinsic Signaling Pathway Involved in Caspase 3 and Caspase 8.

Blood ◽

10.1182/blood.v114.22.4899.4899 ◽

2009 ◽

Vol 114 (22) ◽

pp. 4899-4899

Author(s):

Jumei Shi ◽

Yi Wu ◽

Siqing Wang ◽

Xiuqin Meng ◽

Rong Wei ◽

...

Keyword(s):

Cell Cycle ◽

Cell Growth ◽

Growth Inhibition ◽

Arsenic Trioxide ◽

Signaling Pathway ◽

Molecular Mechanism ◽

Caspase 3 ◽

Myeloma Cell ◽

Caspase 8 ◽

Myeloma Cells

Abstract Abstract 4899 Arsenic trioxide (ATO) is a well-known inhibitor of cell proliferation in certain forms of malignancy and has been successfully used in the treatment of acute promyelocytic leukemia. Preclinical and clinical studies showed that ATO has anti-myeloma effects both as a single agent and in the combination therapy; however, the underlying molecular mechanism remains elusive. This study was performed to evaluate the molecular mechanism underlying its anti-myeloma activities. Cells from OPM2, U266, RPMI8226 myeloma cell lines and patients diagnosed with myeloma (n=6) were cultured with various concentrations of ATO for 4 days. Cell growth and viability were assayed by trypan blue dye exclusion. Cell cycle and apoptosis were analyzed by flow cytometry using CellQuest software and Vybrant Apoptosis Assay Kit. Alterations of the signaling pathways induced by ATO were tested by real-time PCR and western blot. ATO induced potent inhibition of myeloma cell growth compared with untreated control cells. Further investigation showed that ATO down-regulated c-Myc and phosphorylated (p)-Rb, while it up-regulated p53, p21Clip1, and p27Kip1 proteins, resulting in G2/M cell cycle arrest and cell growth inhibition. ATO treatment increased mRNA levels of interferon regulatory factor-1 (IRF-1) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), as well as protein levels of caspase 8 and cleaved caspase 3, indicating involvement of the extrinsic apoptotic pathway. No significant change was detected in the expression levels of Bax, Bcl-xL caspase 9 and Bcl-2, indicating that the intrinsic signaling pathway was not involved. A pan-caspase inhibitor abrogated ATO-induced apoptosis of myeloma cells. Our data suggest that ATO induces apoptosis in MM cells most likely through an extracellular signaling pathway. Disclosures No relevant conflicts of interest to declare.

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