Physiological changes in fruit ripening caused by overexpression of tomato SlAN2, an R2R3-MYB factor

2015 ◽  
Vol 89 ◽  
pp. 24-30 ◽  
Author(s):  
Xia Meng ◽  
Dongyue Yang ◽  
Xiaodong Li ◽  
Shuya Zhao ◽  
Na Sui ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Physiological Changes ◽  
R2r3 Myb ◽  
Myb Factor

scholarly journals The R2R3-MYB Factor FhMYB5 From Freesia hybrida Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis

2019 ◽  
Vol 9 ◽  
Author(s):  
Yueqing Li ◽  
Xiaotong Shan ◽  
Liudi Zhou ◽  
Ruifang Gao ◽  
Song Yang ◽  
...  
Keyword(s):  
Freesia Hybrida ◽  
R2r3 Myb ◽  
Myb Factor

scholarly journals Genome-Wide Characterization of R2R3-MYB Transcription Factors in Pitaya Reveals a R2R3-MYB Repressor HuMYB1 Involved in Fruit Ripening through Regulation of Betalain Biosynthesis by Repressing Betalain Biosynthesis-Related Genes

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1949
Author(s):  
Fangfang Xie ◽  
Qingzhu Hua ◽  
Canbin Chen ◽  
Zhike Zhang ◽  
Rong Zhang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Family ◽  
Fruit Ripening ◽  
Phylogenetic Analyses ◽  
Luciferase Reporter ◽  
Molecular Characteristics ◽  
Genome Data ◽  
Genome Wide ◽  
Hylocereus Undatus ◽  
R2r3 Myb

The MYB (myeloblastosis) superfamily constitutes one of the most abundant transcription factors (TFs) regulating various biological processes in plants. However, the molecular characteristics and functions of MYB TFs in pitaya remain unclear. To date, no genome-wide characterization analysis of this gene family has been conducted in the Cactaceae species. In this study, 105 R2R3-MYB members were identified from the genome data of Hylocereus undatus and their conserved motifs, physiological and biochemical characteristics, chromosome locations, synteny relationship, gene structure and phylogeny were further analyzed. Expression analyses suggested that three up-regulated HuMYBs and twenty-two down-regulated HuMYBs were probably involved in fruit ripening of pitaya. Phylogenetic analyses of R2R3-MYB repressors showed that seven HuMYBs (HuMYB1, HuMYB21, HuMYB48, HuMYB49, HuMYB72, HuMYB78 and HuMYB101) were in clades containing R2R3-MYB repressors. HuMYB1 and HuMYB21 were significantly down-regulated with the betalain accumulation during fruit ripening of ‘Guanhuahong’ pitaya (H. monacanthus). However, only HuMYB1 had R2 and R3 repeats with C1, C2, C3 and C4 motifs. HuMYB1 was localized exclusively to the nucleus and exhibited transcriptional inhibition capacities. Dual luciferase reporter assay demonstrated that HuMYB1 inhibited the expression of betalain-related genes: HuADH1, HuCYP76AD1-1 and HuDODA1. These results suggested that HuMYB1 is a potential repressor of betalain biosynthesis during pitaya fruit ripening. Our results provide the first genome-wide analyses of the R2R3-MYB subfamily involved in pitaya betalain biosynthesis and will facilitate functional analysis of this gene family in the future.

scholarly journals R2R3-MYB Transcription Factor NtMYB330 Regulates Proanthocyanidin Biosynthesis and Seed Germination in Tobacco (Nicotiana tabacum L.)

2022 ◽  
Vol 12 ◽  
Author(s):  
Lu Zhao ◽  
Zhongbang Song ◽  
Bingwu Wang ◽  
Yulong Gao ◽  
Junli Shi ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Transcriptional Activation ◽  
Myb Transcription Factor ◽  
Ga Signaling ◽  
Nicotiana Tabacum L ◽  
Tobacco Seeds ◽  
R2r3 Myb ◽  
Myb Factor ◽  
Mbw Complex

Proanthocyanidins (PAs) are important phenolic compounds and PA biosynthesis is regulated by a ternary MBW complex consisting of a R2R3-MYB regulator, a bHLH factor and a WDR protein. In this study, a tobacco R2R3-MYB factor NtMYB330 was characterized as the PA-specific regulator in which the PA biosynthesis was promoted in the flowers of NtMYB330-overexpressing lines while decreased in the flowers of ntmyb330 mutants. NtMYB330 can interact with flavonoid-related bHLH partner NtAn1b and WDR protein NtAn11-1, and the NtMYB330-NtAn1b complex is required to achieve strong transcriptional activation of the PA-related structural genes NtDFR1, NtANS1, NtLAR1 and NtANR1. Our data reveal that NtMYB330 regulates PA biosynthesis in seeds and affects seed germination, in which NtMYB330-overexpressing lines showed higher PA accumulations in seed coats and inhibited germination, while ntmyb330 mutants had reduced seed coat PAs and improved germination. NtMYB330 affects seed germination possibly through two mechanisms: modulating seed coat PAs to affect coat-imposed dormancy. In addition, NtMYB330 regulates the expressions of abscisic acid (ABA) and gibberellin acid (GA) signaling-related genes, affecting ABA-GA crosstalk and seed germination. This study reveals that NtMYB330 specifically regulates PA biosynthesis via formation of the MBW complex in tobacco flowers and affects germination through adjustment of PA concentrations and ABA/GA signaling in tobacco seeds.

Overexpression of a grapevine R2R3-MYB factor in tomato affects vegetative development, flower morphology and flavonoid and terpenoid metabolism

2009 ◽  
Vol 47 (7) ◽  
pp. 551-561 ◽  
Author(s):  
Ali Mahjoub ◽  
Michel Hernould ◽  
Jérôme Joubès ◽  
Alain Decendit ◽  
Mohamed Mars ◽  
...  
Keyword(s):  
Flower Morphology ◽  
Vegetative Development ◽  
Terpenoid Metabolism ◽  
R2r3 Myb ◽  
Myb Factor

scholarly journals AgMYB1, an R2R3-MYB factor, plays a role in anthocyanin production and enhancement of antioxidant capacity in celery

2021 ◽  
Vol 1 (1) ◽  
pp. 1-12
Author(s):  
Kai Feng ◽  
◽  
Guo-Ming Xing ◽  
Jie-Xia Liu ◽  
Hao Wang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
R2r3 Myb ◽  
Anthocyanin Production ◽  
Myb Factor

Inducing Unconscious Stress

2020 ◽  
Vol 34 (3) ◽  
pp. 192-201
Author(s):  
Melanie M. van der Ploeg ◽  
Jos F. Brosschot ◽  
Markus Quirin ◽  
Richard D. Lane ◽  
Bart Verkuil
Keyword(s):  
Repeated Measures ◽  
Activity Patterns ◽  
Physiological Changes ◽  
Implicit Measures ◽  
Prime Word ◽  
Student Sample ◽  
Priming Task ◽  
Subject Design ◽  
The Relationship ◽  
Stress And Health

Abstract. Stress-related stimuli may be presented outside of awareness and may ultimately influence health by causing repetitive increases in physiological parameters, such as blood pressure (BP). In this study, we aimed to corroborate previous studies that demonstrated BP effects of subliminally presented stress-related stimuli. This would add evidence to the hypothesis that unconscious manifestations of stress can affect somatic health. Additionally, we suggest that these findings may be extended by measuring affective changes relating to these physiological changes, using measures for self-reported and implicit positive and negative affectivity. Using a repeated measures between-subject design, we presented either the prime word “angry” ( n = 26) or “relax” ( n = 28) subliminally (17 ms) for 100 trials to a student sample and measured systolic and diastolic BP, heart rate (HR), and affect. The “angry” prime, compared to the “relax” prime, did not affect any of the outcome variables. During the priming task, a higher level of implicit negative affect (INA) was associated with a lower systolic BP and diastolic BP. No association was found with HR. Self-reported affect and implicit positive affect were not related to the cardiovascular (CV) activity. In sum, anger and relax primes elicited similar CV activity patterns, but implicit measures of affect may provide a new method to examine the relationship between (unconscious) stress and health.

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