scholarly journals CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition

2017 ◽  
Vol 1 (2) ◽  
pp. 1-8 ◽  
Author(s):  
Yuanita Rachmawati ◽  
Ganies Rizaa Aristya ◽  
Budi Setiadi Daryono
Keyword(s):  
Gene Expression ◽  
Normal Condition ◽  
Cucumis Melo ◽  
Stress Condition ◽  
Leaf Tissue ◽  
Adaptive Responses ◽  
Degraded Land ◽  
Biotic Stresses ◽  
Aba Metabolism ◽  
Cucumis Melo L

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.

scholarly journals Pollination Methods and Integrated Fertilizer Influenced the Pollination Rate, Fruit Development, and Quality of Cucumis melo L. under Greenhouse Conditions

2021 ◽  
Vol 13 (23) ◽  
pp. 13300
Author(s):  
Nik Zuraila Nik Hassan ◽  
Siti Zaharah Sakimin ◽  
Noraini Md Jaafar ◽  
Illani Zuraihah Ibrahim
Keyword(s):  
Organic Acid ◽  
Cucumis Melo ◽  
Leaf Tissue ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Yield Quality ◽  
Solids Content ◽  
Pollination Methods ◽  
Cucumis Melo L

This study was conducted to determine the effect of pollination methods (PMs) and integrated fertilizer (IF) application on the pollination rate, yield, and fruit quality of rockmelon (Cucumis melo L. var. Reticulatus cv. Glamour). This factorial experiment was arranged in a split plot design with four replications. Two rates of IF between chemical (CF) and organic (OF) fertilizer were investigated, namely, T1 (100% CF), which served as a control, and T2 (75% CF + 25% OF). The three PMs used in this study were natural (NP), bee (BP), and human (HP) pollination. Data collection included the pollination rate, yield, postharvest quality (soluble solids content, color, firmness, sugar, and organic acid), and macronutrient content in leaf tissue. The results of this study showed that there was an interaction of the pollination rate between PM and IF; however, the number of bees that survived decreased over time. The yield quality showed an interaction between PM and IF for both the fruit diameter and weight, while no interaction between PM and IF for the organic acid and sugar contents was observed. In conclusion, T2 application combined with BP reduced CF utilization, which benefits the development of a sustainable rockmelon production system.

scholarly journals Comparative Analysis of Gene Expression in Two Muskmelon Cultivars (Cucumis melo L.) Under Salt Stress

2014 ◽  
Vol 13 (10) ◽  
pp. 2132-2140 ◽  
Author(s):  
Shi-wei WEI ◽  
Fu-rong ZHANG ◽  
Yi-dong ZHANG ◽  
Li-min WANG ◽  
Jia-bei CHEN ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Comparative Analysis ◽  
Cucumis Melo ◽  
Cucumis Melo L

scholarly journals Evaluasi Deskriptif Umur Panen Melon (Cucumis melo, L) Di Pekanbaru

2018 ◽  
Vol 14 (1) ◽  
pp. 65-71
Author(s):  
Surtinah Surtinah
Keyword(s):  
Organic Matter ◽  
Water Stress ◽  
Growth Regulators ◽  
Cucumis Melo ◽  
Research Method ◽  
Stress Condition ◽  
Environmental Modification ◽  
Water Stress Condition ◽  
Cucumis Melo L ◽  
Descriptive Qualitative

The objective of the study was to examine the factors that caused melon harvesting age in Pekanbaru faster than in the melon-producing regions. The research method used is descriptive qualitative. The results of the discussion found that the factors that influence the harvesting age of melon in Pekanbaru City are the water stress condition, the unfulfilled essential ingredients, the insufficient planting medium of organic matter, the importance of growth regulators that can enlarge the fruit and maintain the age of the plant, environmental modification.

scholarly journals Melonet-DB, a Grand RNA-Seq Gene Expression Atlas in Melon (Cucumis melo L.)

2017 ◽  
Vol 59 (1) ◽  
pp. e4-e4 ◽  
Author(s):  
Ryoichi Yano ◽  
Satoko Nonaka ◽  
Hiroshi Ezura
Keyword(s):  
Gene Expression ◽  
Cucumis Melo ◽  
Rna Seq ◽  
Gene Expression Atlas ◽  
Expression Atlas ◽  
Cucumis Melo L

scholarly journals Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
WIKO ARIF WIBOWO ◽  
MUHAMMAD IMAM FATKHUROHMAN ◽  
BUDI SETIADI DARYONO
Keyword(s):  
Gene Expression ◽  
Cucumis Melo ◽  
Complex Mixture ◽  
Storage Period ◽  
Acyl Transferase ◽  
Gene Encoding ◽  
Distinctive Character ◽  
Gene Characterization ◽  
Cucumis Melo L ◽  
Melon Fruit

Abstract. Wibowo WA, Fatkhurohman MI, Daryono BS. 2020. Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’. Biodiversitas 21: 3041-3046. Melon (Cucumis melo L.) is one of the horticulture commodities that have high economic value and its needs increase continuously. Many new melon cultivars have been assembled to produce a higher quality melon. Melon 'Hikapel' developed by the Laboratory of Genetics and Breeding, Faculty of Biology UGM has distinctive character in the form of a strong aroma. This aroma is a complex mixture of various kinds of volatile compound. One of the main determinant compounds is a volatile ester, synthesized by the alcohol acyl-transferase enzyme encoded by the Cm-AAT1 gene. Characterization of Cm-AAT1 began with isolation of melon rinds to get total RNAs. Synthesis cDNA was conducted with oligo-dT primer, followed by detection of Cm-AAT1 using specific primers. A specific band was sequenced to perform phylogenetic tree. Gene expression from 4 melon cultivars, ‘Hikapel’, ‘Hikadi’, ‘Sun Lady’, and ‘Luna’ analysis was performed using relative quantitative Real-Time PCR. The results of this study showed that Cm-AAT1 owned not only by aromatic cultivars ‘Hikapel’ and ‘Hikadi’, but also owned by non-aromatic cultivars ‘Sun Lady’ and ‘Luna’. Phylogenetic analysis shows a high similarity between Cm-AAT1 on 'Hikapel' and 'Hikadi'. Gene expression analysis on 'Hikapel' increases as the process of fruit ripening during the storage period and it is in contrast to 'Hikadi' at decrease when the fruit began to enter the decay process on day 7th. Expression of Cm-AAT1 on ‘Hikapel’ was higher than ‘Hikadi’ at the peak of fruit maturity.

scholarly journals Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene

2020 ◽  
Vol 27 (2) ◽  
pp. 174
Author(s):  
Eka Wasi’ Al-Mughni ◽  
Budi Setiadi Daryono
Keyword(s):  
Gene Expression ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Phylogenetic Relationships ◽  
Cucumis Melo ◽  
Specific Primer ◽  
Economic Prospect ◽  
Cucumis Melo L

Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars.

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