Microencapsulation of phenolic-enriched extract from cocoa pod husk (Theobroma cacao L.)

2021 ◽  
Vol 386 ◽  
pp. 136-143
Author(s):  
Van Tang Nguyen ◽  
Anh Xuan Tran ◽  
Van Anh Thi Le
2019 ◽  
Vol 1317 ◽  
pp. 012087
Author(s):  
Rachmawaty ◽  
Andi Mu’nisa ◽  
Hasri ◽  
Halifah Pagarra ◽  
Hartati

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6915
Author(s):  
Mustanir Yahya ◽  
Binawati Ginting ◽  
Nurdin Saidi

Increasing production of cocoa (Theobroma cacao L.) leads to a higher environmental burden due to its solid waste generation. Cocoa pod husk, one of the major solid wastes of cocoa production, contains rich bioactive compounds unveiling its valorization potential. With that in mind, our research aimed to explore the biological and antioxidant activities of aqueous extracts from cocoa pod husks. In this present work, cocoa pod husk was extracted using water and subsequentially partitioned using n-hexane, ethyl acetate, and methanol. The antimicrobial investigation revealed that the ethyl acetate solubles were active against the Staphylococcus aureus, Escherichia coli, and Candida albicans, where at a 20% w/v concentration, the inhibition diameters were 6.62 ± 0.10, 6.52 ± 0.02, and 11.72 ± 0.36 mm, respectively. The extracts were found non-toxic proven by brine shrimp lethality tests against Artemia salina with LC50 scores ranging from 74.1 to 19,054.6 μg/mL. The total phenolic content and total flavonoid content were obtained in the range of 47.44 to 570.44 mg/g GAE and 1.96 to 4.34 mg/g QE, respectively. Antioxidant activities of the obtained extracts were revealed by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay with EC50 reached as low as 9.61 μg/mL by the ethyl acetate soluble. Phytochemical screening based on gas chromatography—mass spectroscopy analysis on the sample with the highest antioxidant activities revealed the dominant presence of three phytosterols, namely gamma-sitosterol, stigmasterol, and campesterol.


2020 ◽  
Vol 21 (4) ◽  
pp. 611-616
Author(s):  
Suryadi Pappa ◽  
Abdul Wahid Jamaluddin ◽  
Adryani Ris

This study was aims to exploit the potential waste of cocoa pod (Theobroma cacao L.) as a natural acaricide in ticks (Boophilus microplus). The study was conducted with a two-variable completely randomized design (CRD) with two repetitions. The sample does not differentiate between sex, body weight, and length of about 0.8 - 1 cm. Each 5 ticks were treated as follows: treat 1 extract of 10% cocoa pod husk; treatment 2 20% cocoa pod skin extract; treatment 3 30% cocoa pod husk extract; treatment 4 negative control; treat 5 positive controls. In the first variable the tick was treated with methanol extract, while in the second variable the ethanol extract was treated with 10%, 20%, 30% respectively, negative control and positive control. In treatment I used 25 ticks plus treatment II 25 ticks. Each treatment was repeated to avoid data bias so that a total of 100 ticks were used. The results showed that the cocoa pod husk extract had the best killing power at a concentration of 30% with methanol solvent at 6.21 hours while ethanol solvent at 5.91 hours. The use of extraction materials has not been able to compensate for the use of synthetic materials that can kill in minutes.


2020 ◽  
Vol 7 (2) ◽  
pp. 587-610
Author(s):  
Chris Ayanladun ◽  
Sunday Oke

Cocoa pod husk composites for structural applications face a substantial water absorption problem in which the residual tensile, fatigue, and flexural characteristics always exhibit instant deterioration after immersion in water. Unfortunately, there is an absence of sensitivity analysis research on the composite’s water absorption parameters to establish the crucial parameters that may function as drawbacks. Consequently, this study establishes how the sensitivity analysis of the Fickian’s model parameters can be measured using single and joint parametric variations in the water absorbed at saturation, interactive indicator and the response indicator between patterns using experimental data and literature sources. Results revealed that the most sensitive case study was obtained when the response indicator between patterns was varied with the absorbed quantity in time, accounting for 95.24 % (20 out of 21 cases). Accordingly, composite engineers and designers should use the most sensitive parameter to control design features.


2019 ◽  
Vol 19 (2) ◽  
pp. 49-53
Author(s):  
Binawati Ginting ◽  
Ilham Maulana ◽  
Nurdin Saidi ◽  
Syarifah Yanti Astryna

Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class.


2020 ◽  
Vol 8 (2) ◽  
pp. 210
Author(s):  
Ni Kadek Yeni Dwipayanti ◽  
G.P. Ganda Putra ◽  
Lutfi Suhendra

Cocoa pod husk is a waste of cocoa processing that is large enough and has not been used optimally. Cocoa pod husk waste can be used more optimally by extracting polyphenols compounds and used as natural antioxidants. This research aims to determine the effect of comparison of materials with solvents and maceration time against cocoa pod husk extracts as a source of antioxidants as well as to determine the material comparison with the solvent and the best maceration time for produce cocoa pod husk extract as a source of antioxidants. The study uses a random design of a group of two factors. The first factor is the comparison of materials with solvents consisting of 1:10, 1:15 and 1:20. The second factor is the maceration time consisting of 24, 36 and 48 hours. The data was analyzed its diversity and continued with the Tukey test. The results of the study showed a comparison of material with solvent and maceration time was very influential to the yield, total phenolic and antioxidant capacity of cocoa pod husk extract. The interaction between treatment is very influential on the total phenolic and antioxidant capacity but has no effect on the cocoa pod husk extract yield. The best treatment to produce cocoa pod husk extract as a source of antioxidant is the comparison of materials with solvent 1:20 and maceration time for 48 hours with a yield characteristic 3.10 ± 0.36 percent, total phenolic at 146.67 ± 5.14 mg GAE/g and antioxidant capacity 97.00 ± 2.22 mg GAEAC/g. Keywords: cocoa pod husk extract, comparison of materials with solvents, maceration time, antioxidant.


2018 ◽  
Vol 6 (1) ◽  
pp. 41
Author(s):  
Muhamad Eza Suprapto ◽  
Santi Rosniawaty ◽  
Mira Ariyanti

Cocoa is one of the estate crop whose role is quite important for the national economy, particularly as a provider of employment,  income and foreign exchange. The high export opportunities of cocoa beans has not been followed by the production of cocoa beans in Indonesian. The production of dried cacao  beans in Indonesia is still low. The low productivity of seeds generally caused by  Cherelle wilt of cacao. Wilting occurs due to inability of the cherelle to compete with the other cherelle in absorbing nutrients. Fertilization is an effort to maintain and fulfill the nutrients in the plants. This research is aimed to assess the effect of fertilization using cocoa pod husk and NPK tablets fertilization on cocoa crop yields. This experiment was conducted at PT. PP. Bajabang Indonesia, Cipeundeuy, West Bandung regency from December 2016 to March 2017. The experiment was conducted using a Simple Randomized Block Design that consists 10 treatments: control, single N, P, K fertilizer  297 g/tree, cocoa pod husk  9 kg/tree, cocoa pod husk  9 kg/tree + single N, P, K fertilizer 297 g/tree, cocoa pod husk 18 kg/tree + single N, P, K fertilizer  297 g/tree, cocoa pod husk 9 kg/tree  + NPK tablet fertilizer 20 tablets/tree, NPK tablet fertilizer 20 tablets/tree,  cocoa pod husk  9 kg/tree  +  NPK tablet fertilizer 10 tablets/tree, cocoa pod husk 18 kg/tree + NPK tablet fertilizer 20 tablets/ tree, cocoa pod husk 18 kg/ tree + NPK tablet fertilizer 10 tablets/tree. The result of experiment showed that there was an influence of cocoa pod husk and NPK tablet fertilizer on cocoa fruit weight at 12 MSP, that is cocoa pod husk 9 kg/tree  +  NPK  tablet fertilizer 20  tablets/tree.  Number of cherelle wilted  at 12 MSP with cocoa pod  husk 9 kg/tree  +  NPK  tablet fertilizer 20  tablets/tree.


Author(s):  
R. O. Igbinadolor ◽  
C. V. Bruschi ◽  
A. A. Onilude

Biobased energy and fuels are among the exercisable sustainable energy options mankind has in the not-so-distant future as issues pertaining to global warming and shortfall in fossil fuels loom dark over the planet. The environmental necessity to stop this development by switching to alternative strategies nowadays is generally undisputed. Biofuel made from biomass provide unique environmental economic strategic benefit and can be considered as safe and by and large, the cleanest liquid fuel alternative to fossil fuels. Biofuel produced from Agricultural waste biomass like cocoa (Theobroma cacao L) pod husk shows many potentials advantages in comparison with sugar or starch-based stocks since the latter materials are also food for human and animals. However, the complex nature of this biomass necessitates the use of genetic techniques to produce engineered organisms that are able to transform this polymer into the desired product. With Bioinformatics tools using NCBI BLAST programme, two genes XL1 and XL2 encoding pentose utilization were isolated from the genomic DNA of Pichia stipitis (CBS 6054) and two primers each were designed to span the full coding region of these genes with attached enzymes restriction sites using DNA strider 1.4f7 and Macplasmap programmes. PCR reactions were carried out on 120hg of the isolated genomic DNA for 30 cycles using the DNA Gotaq polymerase enzyme. The amplified PCR fragments were introduced into plasmid vectors pGAPZA and pVT100-U respectively and the constructs were then used to transform the selected ethanol-producing strain of S. cerevisiae (BY4743) isolated from degrading cocoa pod husk biomass meant to serve as starter for biofuel production from cocoa pod husk hydrolysate.


Author(s):  
Jerome A. Dogbatse ◽  
A. Arthur ◽  
I. Amoaku-Attah ◽  
A. K. Quaye ◽  
S. Konlan ◽  
...  

Aim: To study the effects of cocoa pod husk based compost: Soil mixtures on growth of cocoa (Theobroma cacao L.) seedlings. Study Design: The experiment was laid out in a Complete Randomized Design with four replications. Place and Duration of Study: The experiment was conducted at the main nursery of Cocoa Research Institute of Ghana at New Tafo - Akim (06°13’ N and 00°22’ W) in the Eastern Region of Ghana, between November, 2016 and December 2017. Methodology: Surface soil classified as Ferric Lixisols together with four compost types produced from cocoa pod husk, poultry manure and Panicum maximum was used to fill polythene bags. A Soil alone (T1) and Soil + standard foliar fertilizer (T2) with a four compost:soil mixture treatments namely T3 - 1:1 w/w, T4 - 1:2 w/w, T5 -1:3 w/w and T6 - 2:1 w/w were tested. Each of the four compost types was used for T3 to T6. Surface soil, poultry manure, compost and compost-soil mixtures were carried out using standard laboratory procedures. Mixed hybrid cocoa seedlings were raised and growth evaluated monthly. Results: Interaction effect was observed between compost types and compost-soil mixtures on dry matter yield of cocoa seedlings. Cocoa seedlings grown in T5 of compost 1 had the highest dry matter yield. Higher shoot: root ratio of cocoa seedlings were observed in compost 1. The order of desirability for the four compost types in terms of compost-soil mixtures was compost:soil (1:3 w/w) > compost:soil (1:2 w/w) > compost:soil (2:1 w/w) > compost:soil (1:1 w/w). CMPT1 will ensure more vigorous cocoa seedlings growth after transplanting and subsequently, higher establishment rate. Conclusion: The optimum mixture of compost and soil for growing cocoa seedlings under limited availability of fertile surface soil is compost 1 mixed with surface soil at the ratio of 1:3.


2017 ◽  
Vol 10 (30) ◽  
pp. 1493-1500 ◽  
Author(s):  
Dalma Marsiglia-Lopez ◽  
Malena Ramirez-Uribe ◽  
Angel Gonzalez-Delgado ◽  
Karina Ojeda-Delgado ◽  
Eduardo Sanchez-Tuiran

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