Comparative analysis of the chemical treatments used in keratin extraction from red sheep’s hair and the cell viability evaluations of this keratin for tissue engineering applications

Poly(vinylidene fluoride) microparticles with diameters between 0.80 and 5.50 μm were produced. Cell viability shows their suitability for tissue engineering.

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Design of graphene oxide/gelatin electrospun nanocomposite fibers for tissue engineering applications

RSC Advances ◽

10.1039/c6ra23986b ◽

2016 ◽

Vol 6 (110) ◽

pp. 109150-109156 ◽

Cited By ~ 14

Author(s):

Sakthivel Nagarajan ◽

Céline Pochat-Bohatier ◽

Catherine Teyssier ◽

Sébastien Balme ◽

Philippe Miele ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Graphene Oxide ◽

Cell Viability ◽

Significant Influence ◽

Cell Attachment ◽

Electrospun Fibers ◽

Engineering Applications ◽

Nanocomposite Fibers

2D graphene oxide (GO) is used to enhance the mechanical properties of gelatin electrospun fibers. The GO does not show any significant influence on cell viability and cell attachment even though the expression of osteoblast gene is affected.

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Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone with Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications

Nanomaterials ◽

10.3390/nano10030428 ◽

2020 ◽

Vol 10 (3) ◽

pp. 428 ◽

Cited By ~ 3

Author(s):

Carolina Angulo-Pineda ◽

Kasama Srirussamee ◽

Patricia Palma ◽

Victor M. Fuenzalida ◽

Sarah H. Cartmell ◽

...

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Cell Viability ◽

Reduced Graphene Oxide ◽

Human Cell ◽

Human Mesenchymal Stem Cells ◽

Fold Increase ◽

Cellular Mechanisms ◽

Engineering Applications ◽

Reduced Graphene

Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated S. aureus on the surface of scaffolds. Notably, the same ES regime also produced a four-fold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.

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Electrospun PCL, gold nanoparticles, and soy lecithin composite material for tissue engineering applications

Journal of Biomaterials Applications ◽

10.1177/0885328218815807 ◽

2018 ◽

Vol 33 (7) ◽

pp. 979-988 ◽

Cited By ~ 2

Author(s):

Toni Matson ◽

Jonathan Gootee ◽

Colten Snider ◽

John Brockman ◽

David Grant ◽

...

Keyword(s):

Tissue Engineering ◽

Gold Nanoparticles ◽

Cell Viability ◽

Critical Role ◽

Chemical Properties ◽

Soy Lecithin ◽

Engineering Applications ◽

Significant Difference ◽

Fiber Mesh ◽

And Chemical Properties

Soy lecithin has been shown to play a critical role in cell signaling and cellular membrane structure. In addition, it has been shown to increase biocompatibility, hydrophilicity, and decrease cytotoxicity. Gold nanoparticles have also shown to improve cellularity. Lecithin, gold nanoparticles, and polycaprolactone (PCL) solutions were electrospun in order to develop unique mesh materials for the treatment of osteoarthritis. The electrospinning parameters were optimized to achieve different solution ratios for fiber optimization. The amount of lecithin mixed with PCL varied from 30 wt.% to 50 wt.% . Gold nanoparticles (1% to 10% concentrations) were also added to lecithin-PCL mixture. The mechanical and chemical properties of the fiber mesh were analyzed via contact angle test, tensile mechanical tests, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). Cell viability was measured using a WST-1 Assay. Scanning electron microscopy confirmed the successful formation of fiber mesh. The compositions of 40% soy lecithin with PCL in 40% solvent (40:40) resulted in the most well-formed fiber mesh. DSC melt temperatures were statically insignificant; uniaxial stresses and the moduli resulted in no significant difference between the test composition and pristine PCL compositions. WST-1 assay revealed all compositions were non-cytotoxic. Overall, the addition of lecithin increased hydrophilicity while maintaining cell viability and the mechanical and chemical properties of PCL. This study demonstrated that it is possible to successfully electrospin a lecithin, gold nanoparticle, and polycaprolactone scaffold for tissue engineering applications.

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A Tailored Biomimetic Hydrogel as Potential Bioink to Print a Cell Scaffold for Tissue Engineering Applications: Printability and Cell Viability Evaluation

Applied Sciences ◽

10.3390/app11020829 ◽

2021 ◽

Vol 11 (2) ◽

pp. 829

Author(s):

Shyuan-Yow Chen ◽

Yung-Chieh Cho ◽

Tzu-Sen Yang ◽

Keng-Liang Ou ◽

Wen-Chien Lan ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Viability ◽

Three Dimensional ◽

Cell Encapsulation ◽

Pluronic F127 ◽

Layer By Layer ◽

Engineering Applications ◽

Printing Quality ◽

A Cell ◽

Printing Parameters

The present study established a maximum standard for printing quality and developed a preliminary ideal index to print three-dimensional (3D) construct using the Gly-Arg-Gly-Asp (GRGD) peptide modified Pluronic-F127 hydrogel (hereafter defined as 3DG bioformer (3BE)) as bioink. In addition, the biocompatibility of 3BE for 3D printing applications was carefully investigated. For biocompatibility study and ideal printing parameter, we used the formulation of 3BE in three different concentrations (3BE-1: 25%, 3BE-2: 30%, and 3BE-3: 35%). The 3BE hydrogels were printed layer by layer as a cube-like construct with all diameters of the needle head under the same feed (100 mm/s). The printing parameters were determined using combinations of 3BE-1, 3BE-2, and 3BE-3 with three different standard needle sizes (Φ 0.13 mm, Φ 0.33 mm, and Φ 0.9 mm). The printed constructs were photographed and observed using optical microscopy. The cell viability and proliferation were evaluated using Live/Dead assay and immunofluorescence staining. Results showed that a stable of printed line and construct could be generated from the 3BE-3 combinations. Cytotoxicity assay indicated that the 3BE hydrogels possessed well biocompatibility. Bioprinting results also demonstrated that significant cell proliferation in the 3BE-3 combinations was found within three days of printing. Therefore, the study discovered the potential printing parameters of 3BE as bioink to print a stable construct that may also have high biocompatibility for cell encapsulation. This finding could serve as valuable information in creating a functional scaffold for tissue engineering applications.

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