Time course of nutritional and functional property changes in egg yolk from laying hens fed docosahexaenoic acid-rich microalgae

Abstract Enrichment of docosahexaenoic-acid (DHA) into eggs of laying-hens may be limited by the availability phospholipids as a deposition sink. The present study was to determine if dietary supplementations of phospholipid-component molecules or synthesis-enhancers: choline, serine, and ethanolamine could elevate phospholipid and DHA enrichment in the eggs and tissues of hens. A total of 50-White-Leghorn-Shavers (42-wk old) were individually caged and divided into 5 groups (n = 10/group). The 5 groups of hens were fed the following diets for 3 wk: Diet-1 = a corn soybean-meal basal-diet, Diet-2 = Diet-1 + 4%-microalgae (Aurantiochytrium, Heliae, Gibert, AZ, 1.81 g-DHA/kg) + choline-chloride (26.3 g/kg diet, 60% purity, DSM-Co., Basel, Switzerland), Diet-3 = Diet-2 + 1.41% of L-serine (100% purity, Ajinomoto-Co., Inc., Kawasaki, Japan), Diet-4 = Diet-2 + 100 mg of ethanolamine/kg (99% purity, Sigma-Aldirch-Co., St Loius, MO), and Diet-5 = Diet-3 + 100 mg of ethanolamine/kg. At the end of study, eggs, liver, ovary, and adipose samples were collected from 6 hens/group for biochemical analysis. Data were analyzed by one-way ANOVA. Compared with Diet-1, Diet-2 enhanced (P < 0.05) DHA concentrations in egg yolk and liver by 213 mg/egg and 2.98 mg/g tissue, respectively, but decreased (P < 0.05) total phospholipid-concentrations of yolk and liver by 50%, and hepatic-mRNA levels of elongases-2/5 and desaturases-4/6 by 25–50%. Compared with Diet-2, Diet-5 enhanced (P < 0.05) DHA (by 20%) and phospholipid (by 40%) concentrations in the egg yolk, and upregulated (P < 0.05) lipid-metabolism genes involved in the citicoline (CDP, up-to-3-fold) and CDP-ethanolamine (up-to-2.5-fold) pathways in the liver and ovary-tissue. In comparison, Diets-3 and 4 resulted in only 3–11% higher (P < 0.05) DHA-concentrations in the liver over Diet-2. In conclusion, feeding hens a high DHA and choline diet enriched DHA in the egg yolk and down-regulated lipogenesis-gene-expression in the tissues. Supplementing the diet with extra-serine and ethanolamine further-enhanced the DHA enrichment in the egg yolk and restored the high DHA-mediated changes in the gene-expression. (Supported in part by DOE-MAGIC-grant DE-EE0007091, USDA-grant 2019-69012-29905, and Cornell-University-Hatch-grants NYC-127419/127302).

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Effect of Dietary Incorporation of Linseed Alone or Together with Tomato-Red Pepper Mix on Laying Hens’ Egg Yolk Fatty Acids Profile and Health Lipid Indexes

Nutrients ◽

10.3390/nu11040813 ◽

2019 ◽

Vol 11 (4) ◽

pp. 813 ◽

Cited By ~ 11

Author(s):

Besma Omri ◽

Raja Chalghoumi ◽

Luana Izzo ◽

Alberto Ritieni ◽

Massimo Lucarini ◽

...

Keyword(s):

Fatty Acids ◽

Docosahexaenoic Acid ◽

Laying Hens ◽

Dietary Treatment ◽

Egg Yolk ◽

Red Pepper ◽

Standard Diet ◽

Fatty Acids Profile ◽

Tomato Paste ◽

Diet Control

This study evaluated the effect of linseed incorporation in laying hens’ feed (alone or along with a tomato-red pepper mix) on laying hens’ egg yolk fatty acids profile, as well as on their atherogenic (IA) and thrombogenic (IT) health lipid indexes, and the ratio between the hypocholesterolemic and hypercholesterolemic fatty acids (HH). Sixty 27 weeks-old Novogen White laying hens were divided into three groups and given 100 g/hen/day of a standard diet (Control, C) containing 4.5% of ground linseed (Linseed diet, L), containing 1% of dried tomato paste and 1% sweet red pepper (Lineseeds-Tomato-Pepper, LTP). The linseed dietary inclusion significantly reduced the egg yolk content of palmitic acid from 25.41% (C) to 23.43% (L) and that of stearic acid from 14.75% (C) to 12.52% (L). Feeding 4.5% ground linseed did not affect the egg yolk content of α-Linolenic acid but significantly increased the egg yolk concentration of eicosapentaenoic acid (EPA) from 0.011% (C) to 0.047% (L) and that of docosahexaenoic acid (DHA) from 1.94% (C) to 2.73% (L). The IA and the HH were not affected (p > 0.05) by the dietary addition of linseed, whereas the IT decreased (p < 0.05) from 1.16 (C) to 0.86 (L). Adding tomato-sweet red pepper mix to the linseed-supplemented feed did not affect the measured parameters as compared to the linseed dietary treatment.

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Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in laying Leghorn hens

The Journal of Agricultural Science ◽

10.1017/s0021859605004867 ◽

2004 ◽

Vol 142 (6) ◽

pp. 683-690 ◽

Cited By ~ 13

Author(s):

C. H. CHENG ◽

T. F. SHEN ◽

W. L. CHEN ◽

S. T. DING

Keyword(s):

Transcription Factors ◽

Fatty Acid ◽

Docosahexaenoic Acid ◽

Acid Deposition ◽

Egg Production ◽

Laying Hens ◽

Regulatory Element ◽

Egg Yolk ◽

Total Fatty Acids ◽

Egg Yolks

The purpose of the current study was to determine the effects of dietary docosahexaenoic acid (DHA) on fatty acid deposition in egg yolk and various tissues of laying Leghorn hens, and on the expression of hepatic lipogenesis-related transcription factors. There were three treatments with 10 Leghorn hens per treatment. Diets were based on maize-soybean meal (ME: 12 MJ/kg; CP: 171 g/kg; Ca: 34 g/kg) supplemented with 0 (plus 20 g butter/kg), 5 g (plus 15 g butter/kg) or 20 g algal DHA oil/kg. The egg production of the birds was not affected by dietary DHA oil (P>0·05). The DHA content in egg yolks of the laying hens increased significantly (P<0·01) with the dietary supplementation of DHA. The DHA content of the total fatty acids in the egg yolk of laying hens was 4±16, 11±43 and 20±90 g/kg for 0, 5 and 20 g/kg DHA treatments, respectively for the first week, and 6±16, 18±49 and 31±86 g/kg for the second week. Therefore, algal DHA oil can be utilized by laying Leghorn hens to enhance egg yolk DHA content and produce high DHA eggs. The concentrations of triacylglycerol (TG) and cholesterol in plasma of laying Leghorn hens were not affected by dietary DHA treatments (P>0·005). The DHA content in plasma and livers of laying hens was increased with the addition of DHA in the diet (P<0·05). The DHA content in the skeletal muscle of Leghorn hens was also increased with the increase in dietary DHA. The data indicate that dietary DHA oil can be incorporated into plasma, egg yolks and various tissues. The mRNA concentrations of the lipogenesis-related transcription factors, sterol regulatory element binding protein 1 (SREBP1) and SREBP2, in the livers of laying Leghorn hens were not affected by the supplement of algal DHA oil for 2 weeks, suggesting that the expressions of these transcription factors are tightly controlled and not sensitive to DHA treatments in laying hens.

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Dietary choline and phospholipid supplementation enhanced docosahexaenoic acid enrichment in egg yolk of laying hens fed a 2% Schizochytrium powder-added diet

Poultry Science ◽

10.3382/ps/pex095 ◽

2017 ◽

Vol 96 (8) ◽

pp. 2786-2794 ◽

Cited By ~ 6

Author(s):

H. Wang ◽

H.J. Zhang ◽

X.C. Wang ◽

S.G. Wu ◽

J. Wang ◽

...

Keyword(s):

Docosahexaenoic Acid ◽

Laying Hens ◽

Egg Yolk ◽

Dietary Choline

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Schizochytrium limacinum SR-21 as a source of docosahexaenoic acid: optimal growth and use as a dietary supplement for laying hens

Australian Journal of Agricultural Research ◽

10.1071/ar05099 ◽

2006 ◽

Vol 57 (1) ◽

pp. 13 ◽

Cited By ~ 19

Author(s):

H. J. Chin ◽

T. F. Shen ◽

H. P. Su ◽

S. T. Ding

Keyword(s):

Docosahexaenoic Acid ◽

Dietary Supplement ◽

Egg Production ◽

Laying Hens ◽

Optimal Growth ◽

Egg Yolk ◽

Culture Conditions ◽

Marine Fungus ◽

Lipogenic Genes ◽

Schizochytrium Limacinum

Culture conditions for the marine fungus Schizochytrium limacinum SR-21 (SR-21) to produce microbial docosahexaenoic acid (DHA) were evaluated, and the practicality of using this fungus product as a dietary supplement for laying Leghorn hens was investigated. The data showed that the cultured fungus produced high biomass and DHA. It generated 584 mg DHA/L of culture at the end of a 6-day culture. The fungus grew better at 25ºC than at 20ºC or 30ºC. With an increase in glucose concentration from 1% to 5% in the culture medium, biomass and DHA production were enhanced. A 6% glucose treatment reduced the biomass production compared with 5% glucose. A bioreactor was used to mass-produce SR-21. The biomass was increased from 1.12 g/L at Day 0 to 12 g/L at Day 4. We established optimal culture conditions of 5% glucose, 2% sea salt, and 1% yeast extract for SR-21. Three concentrations of dried fungal meal (0, 1, or 3% in the diet) were fed to birds over a 3-week period. There were no negative effects of 1 and 3% dietary SR-21 on egg production, egg weight, and egg yolk weight. The DHA content of yolk was increased by the dietary supplementation with the fungal meal both in the 1 and 3% treatments. Dietary fungal meal treatments increased the DHA concentrations of liver and plasma. However, dietary DHA enrichment had no effect on the expression of hepatic lipogenic genes in laying hens.

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PSVIII-2 Antibody yield for polyclonal egg yolk antibody (IgY) production in laying hens

Journal of Animal Science ◽

10.1093/jas/skaa278.455 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 252-253

Author(s):

Cherrie Nolden ◽

Abbey Grisham ◽

Dan Schaefer ◽

Matt Akins ◽

Mark Cook

Keyword(s):

Repeated Measures ◽

Laying Hens ◽

Egg Yolk ◽

Interleukin 10 ◽

Cpg Odn ◽

Slow Decline ◽

Egg Yolks ◽

Egg Yolk Antibody ◽

To Receive ◽

Phosphate Buffered Saline

Abstract Antibody production in egg yolks of immunized laying hens is an alternative to conventional mammalian production. Antibody yield peak and duration have not been described for immunoglobulin Y technology using Freund’s incomplete adjuvant (FIA) and C-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) without the inclusion of Freund’s complete adjuvant for enhancing the immune response to an interleukin-10 (IL-10) peptide. This study sought to describe the antibody titer production for an 8 amino acid sequence from the surface of the bovine IL-10 protein (VMPQAENG) as the antigen emulsified with CpG-ODN and FIA in phosphate buffered saline (PBS). 60 hens were assigned to receive the complete vaccine (Peptide), 20 received the vaccine without the IL-10 peptide (Control), and 8 received a PBS injection (Blank). Hens were immunized with 0.25 mL in 4 locations, each breast and each thigh on days 1, 15 and 29. The complete vaccine delivered 0.6 mg IL-10 peptide, 8 µg CpG-ODN, and 0.33 mL FIA per hen on each vaccination day. Eggs were collected regularly until 175 days after the first immunization and the anti IL-10 peptide activities of the yolk were determined by ELISA. Egg titers by treatment were analyzed with a repeated measures ANOVA in SAS. The supplementation of FIA with CpG-ODN produced high titers, of over 100 µg of antibody per mL of yolk (µg Ab/mL yolk), around day 33 through day 76, with a slow decline through day 175 when average titers remained above 40 µg Ab/mL yolk. Peptide egg titers were significantly higher than Blank or Control titers from day 31 though day 175 (P < 0.0001). Titers recovered from Marcq et al. (2015) with similar methods were 1.5 to 7 times lower than these results over the same number of days.

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Use of achiote (Bixa orellana L.) seed powder as pigment of the egg yolk of laying hens

The Journal of Applied Poultry Research ◽

10.1016/j.japr.2021.100154 ◽

2021 ◽

pp. 100154

Author(s):

Y. Martínez ◽

C.E. Orozco ◽

R.M. Montellano ◽

M. Valdivié ◽

C.A. Parrado

Keyword(s):

Laying Hens ◽

Egg Yolk ◽

Bixa Orellana

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Arthrospira Platensis (Spirulina) Supplementation on Laying Hens’ Performance: Eggs Physical, Chemical, and Sensorial Qualities

Foods ◽

10.3390/foods8090386 ◽

2019 ◽

Vol 8 (9) ◽

pp. 386 ◽

Cited By ~ 1

Author(s):

Omri ◽

Amraoui ◽

Tarek ◽

Lucarini ◽

Durazzo ◽

...

Keyword(s):

Laying Hens ◽

Control Diet ◽

Dietary Supplementation ◽

Basal Diet ◽

Egg Yolk ◽

Arthrospira Platensis ◽

Cholesterol Concentration ◽

Physical Chemical ◽

Egg Yolks ◽

The Impact

The present study evaluated the effects of dietary supplementation of spirulina on laying hens’ performances: Eggs’ physical, chemical, and sensorial qualities. A total of 45 Lohman White hens, 44 weeks of age, were randomized into 3 groups of 15 birds. Hens were given 120 g/d of a basal diet containing 0% (control), 1.5%, and 2.5% of spirulina for 6 weeks. Albumen height and consequently Haugh unit were significantly affected by dietary supplementation of spirulina (p < 0.05) and by weeks on diet (p < 0.05). This supplement did not affect (p > 0.05) egg yolk weight or height. However, spirulina increased egg yolk redness (a*) from 1.33 (C) to 12.67 (D1) and 16.19 (D2) and reduced (p < 0.05) the yellowness (b*) parameter from 62.1(C) to 58.17 (D1) and 55.87 (D2). Egg yolks from hens fed spirulina were darker, more red, and less yellow in color than egg yolks from hens fed the control-diet (p < 0.0001). However, spirulina did not affect (p > 0.05) egg yolks’ total cholesterol concentration. In conclusion, a significant enhancement of egg yolk color was found in response to spirulina supplementation. Further investigations are needed to evaluate the impact of spirulina on egg yolks’ fatty acids profile.

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