scholarly journals PSVIII-2 Antibody yield for polyclonal egg yolk antibody (IgY) production in laying hens

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 252-253
Author(s):  
Cherrie Nolden ◽  
Abbey Grisham ◽  
Dan Schaefer ◽  
Matt Akins ◽  
Mark Cook
Keyword(s):  
Repeated Measures ◽  
Laying Hens ◽  
Egg Yolk ◽  
Interleukin 10 ◽  
Cpg Odn ◽  
Slow Decline ◽  
Egg Yolks ◽  
Egg Yolk Antibody ◽  
To Receive ◽  
Phosphate Buffered Saline

Abstract Antibody production in egg yolks of immunized laying hens is an alternative to conventional mammalian production. Antibody yield peak and duration have not been described for immunoglobulin Y technology using Freund’s incomplete adjuvant (FIA) and C-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) without the inclusion of Freund’s complete adjuvant for enhancing the immune response to an interleukin-10 (IL-10) peptide. This study sought to describe the antibody titer production for an 8 amino acid sequence from the surface of the bovine IL-10 protein (VMPQAENG) as the antigen emulsified with CpG-ODN and FIA in phosphate buffered saline (PBS). 60 hens were assigned to receive the complete vaccine (Peptide), 20 received the vaccine without the IL-10 peptide (Control), and 8 received a PBS injection (Blank). Hens were immunized with 0.25 mL in 4 locations, each breast and each thigh on days 1, 15 and 29. The complete vaccine delivered 0.6 mg IL-10 peptide, 8 µg CpG-ODN, and 0.33 mL FIA per hen on each vaccination day. Eggs were collected regularly until 175 days after the first immunization and the anti IL-10 peptide activities of the yolk were determined by ELISA. Egg titers by treatment were analyzed with a repeated measures ANOVA in SAS. The supplementation of FIA with CpG-ODN produced high titers, of over 100 µg of antibody per mL of yolk (µg Ab/mL yolk), around day 33 through day 76, with a slow decline through day 175 when average titers remained above 40 µg Ab/mL yolk. Peptide egg titers were significantly higher than Blank or Control titers from day 31 though day 175 (P < 0.0001). Titers recovered from Marcq et al. (2015) with similar methods were 1.5 to 7 times lower than these results over the same number of days.

scholarly journals Arthrospira Platensis (Spirulina) Supplementation on Laying Hens’ Performance: Eggs Physical, Chemical, and Sensorial Qualities

Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 386 ◽  
Author(s):  
Omri ◽  
Amraoui ◽  
Tarek ◽  
Lucarini ◽  
Durazzo ◽  
...  
Keyword(s):  
Laying Hens ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Egg Yolk ◽  
Arthrospira Platensis ◽  
Cholesterol Concentration ◽  
Physical Chemical ◽  
Egg Yolks ◽  
The Impact

The present study evaluated the effects of dietary supplementation of spirulina on laying hens’ performances: Eggs’ physical, chemical, and sensorial qualities. A total of 45 Lohman White hens, 44 weeks of age, were randomized into 3 groups of 15 birds. Hens were given 120 g/d of a basal diet containing 0% (control), 1.5%, and 2.5% of spirulina for 6 weeks. Albumen height and consequently Haugh unit were significantly affected by dietary supplementation of spirulina (p < 0.05) and by weeks on diet (p < 0.05). This supplement did not affect (p > 0.05) egg yolk weight or height. However, spirulina increased egg yolk redness (a*) from 1.33 (C) to 12.67 (D1) and 16.19 (D2) and reduced (p < 0.05) the yellowness (b*) parameter from 62.1(C) to 58.17 (D1) and 55.87 (D2). Egg yolks from hens fed spirulina were darker, more red, and less yellow in color than egg yolks from hens fed the control-diet (p < 0.0001). However, spirulina did not affect (p > 0.05) egg yolks’ total cholesterol concentration. In conclusion, a significant enhancement of egg yolk color was found in response to spirulina supplementation. Further investigations are needed to evaluate the impact of spirulina on egg yolks’ fatty acids profile.

scholarly journals Fatty Acid Composition and β-Carotene Content in Egg Yolk of Laying Hens Fed with Linseed, Paprika and Marigold

2016 ◽  
Vol 65 (1-2) ◽  
pp. 15-22 ◽  
Author(s):  
Nedeljka Spasevski ◽  
Dušica Čolović ◽  
Slađana Rakita ◽  
Predrag Ikonić ◽  
Olivera Đuragić ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Laying Hens ◽  
Egg Yolk ◽  
Control Group ◽  
Sunflower Meal ◽  
Carotene Content ◽  
Natural Pigments ◽  
Egg Yolks ◽  
Experimental Group ◽  
Β Carotene

SummaryEggs enriched with n-3 fatty acids and natural pigments are desirable in human food chain due to numerous benefits for health. In this study, we investigated the effects of inclusion of co-extruded linseed and sunflower meal into laying hens’ diet and replacement of synthesized pigment with paprika and marigold flower as sources of natural pigments. Four hundred 18–wk-old Bovan laying hens divided in two groups (control and experiment) were fed for 12 weeks. Fatty acids and β-carotene were monitored every fourth week and successively analyzed. Significantly (p<0.05) increased content of α-linolenic acid in egg yolk in the experimental group in comparison to the control group was observed with addition of linseed, paprika and marigold in laying hens’ nutrition. Nearly the same tendency was observed in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) content. Supplementation of the co-extruded linseed and sunflower meal into laying hens’ diet demonstrated significantly (p<0.05) lower n-6/n-3 FA ratio in experimental group compared to control group. The obtained results showed that the synthetic pigment added to laying hens’ diet provided better pigmentation of egg yolks after one and two months of the feeding trial than paprika and marigold flower. The concentration of β-carotene in egg yolks increased in experimental group after three months of the treatment, and that was the first time that concentrations of β-carotene did not significantly (p < 0.05) differ among control and experimental group, which shows that paprika and marigold flower as sources of natural pigments can successfully replace synthetic pigment. We shall continue research in this field with other supplemented mixtures, as there are indices for other potential feed enhancements among oily seeds and herbs.

scholarly journals Effect of Feeding Achiote Seed on Egg-Yolk Pigmentation

1969 ◽  
Vol 46 (2) ◽  
pp. 91-96
Author(s):  
F. T. Landagora
Keyword(s):  
Drinking Water ◽  
Puerto Rican ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Yolk ◽  
Egg Yolks ◽  
Orange Color ◽  
Effect Of Feeding ◽  
Basal Ration

The yolk-coloring effect of "achiote" seed was investigated. Hens fed a ration supplemented with whole achiote seed laid eggs with deep-colored yolks. The red-orange color that was observed in the egg yolks of the achiote group persisted in both fresh and boiled eggs. Ground achiote seed fed to laying hens did not impart color to the egg yolks when added to the basal ration or when mixed with the drinking water given. The addition of whole achiote seed to the ration fed to laying hens not only colored the egg yolks more deeply, but also increased egg production. Since Puerto Rican consumers prefer eggs with deeper colored yolks this rinding has potential commercial significance.

scholarly journals The effect of astaxanthin and lycopene on the content of fatty acids in chicken egg yolks

2020 ◽  
Vol 11 (4) ◽  
pp. 568-571
Author(s):  
L. V. Shevchenko ◽  
V. A. Davydovych ◽  
V. O. Ushkalov ◽  
S. V. Midyk ◽  
V. M. Mykhalska
Keyword(s):  
Fatty Acids ◽  
Palmitoleic Acid ◽  
Laying Hens ◽  
Lipid Fraction ◽  
Egg Yolk ◽  
Monounsaturated Fatty Acids ◽  
Eicosenoic Acid ◽  
High Line ◽  
Egg Yolks ◽  
Chicken Eggs

Carotenoids that do not have provitamin activity – lycopene and astaxanthin can accumulate in the yolks of chicken eggs and give them colour, as well as affect the metabolism of lipids and fatty acids, which determine the biological value and functional capacity of such products. The aim of this study was to determine the fatty acid composition of egg yolk lipids by adding oily extracts of lycopene (20, 40 and 60 mg/kg feed) or astaxanthin (10, 20 and 30 mg/kg feed) to the diet of laying hens. 45 High Line W36 chickens at the age of 24 weeks were used for the experiment. It was found that the addition of lycopene at doses of 20, 40 and 60 mg/kg and astaxanthin at doses of 10, 20 and 30 mg/kg of feed for 30 days did not significantly affect the ratio of saturated and most monounsaturated fatty acids in egg yolks and Σ SFA and Σ MUFA. Lycopene at a dose of 20 mg/kg of feed reduced the content of cis-11-eicosenoic acid, and astaxanthin at a dose of 10 mg/kg of feed reduced the content of palmitoleic acid by increasing the proportion of cis-10-heptadecenoic acid in the lipids of egg yolks. Addition of lycopene to the feed of laying hens at a dose of 20 mg/kg of feed caused a decrease in the particles of linoleic, which belongs to ω6 PUFA, and cis-eicosenoic acids. Astaxanthin enrichment of the diet of laying hens at a dose of 30 mg/kg reduced the proportion of cis-4,7,10,13,16,19-docosahexaenoic acid, which belongs to ω3 PUFA, in the lipids of the yolks. Σ PUFA decreased in the lipid fraction of yolks only under the influence of lycopene supplements at a dose of 20 mg/kg of feed. Feeding of laying hens with lycopene and astaxanthin supplements did not affect Σ ω3 PUFA and Σ ω6 PUFA. Lycopene at a dose of 20 mg/kg decreased, and astaxanthin at a dose of 30 mg/kg of feed increased ω3/ω6 PUFA in lipids of egg yolks. The results of the research can be used to select oils in combination with carotenoids of natural origin in the diet of chickens during the creation of a model of enrichment of egg yolks by individual representatives of ω3 PUFA and ω6 PUFA.

scholarly journals The effect of a diet supplemented with sea-buckthorn pomace on the colour and viscosity of the egg yolk

2017 ◽  
Vol 86 (3) ◽  
pp. 303-308 ◽  
Author(s):  
Petr Dvořák ◽  
Pavel Suchý ◽  
Eva Straková ◽  
Jana Doležalová
Keyword(s):  
Laying Hens ◽  
Egg Yolk ◽  
Sea Buckthorn ◽  
Control Group ◽  
Lower Viscosity ◽  
Cielab System ◽  
Egg Yolks ◽  
Valuable Product ◽  
Orange Colour ◽  
Colour Indices

Sea-buckthorn pomace is a very valuable product which contains not only important nutrients but also high-quality oils. The question addressed in the present study was to what extent the diet containing the sea-buckthorn pomace would affect the viscosity and colour of egg yolk measured in the CIELAB system. The feeding mixture for laying hens was supplemented with 20; 50 and 100 g∙kg-1 of sea-buckthorn pomace. As a result, colour indices of the egg yolk such as L*, a* and b* changed significantly (α = 0.01). The greatest relative enhancement was observed for indicator a* for the red colour. Visually, this corresponds to the more intense orange colour of the egg yolk. The addition of sea-buckthorn pomace to the diet for laying hens resulted in a larger increase in indicator ∆E* (CIE total colour difference) compared to the control group. Colour indicator hab is the only indicator whose value oscillated around that determined for the control group. The addition of sea-buckthorn pomace to the diet resulted in an increase in colour indices a*, b* and C*ab. Indicator ∆E* also increased significantly with an increasing amount of sea-buckthorn pomace in a diet. Egg yolks were darker, had more intense red and yellow colours, and showed lower viscosity which are all features preferred by the consumer.

scholarly journals Enrichment of table eggs with lutein

Poljoprivreda ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 56-63
Author(s):  
Gordana Kralik ◽  
Olivera Galović ◽  
Polonca Margeta ◽  
Danica Hanžek ◽  
Manuela Grčević ◽  
...  
Keyword(s):  
Laying Hens ◽  
Thiobarbituric Acid ◽  
Egg Yolk ◽  
Egg White ◽  
Control Group ◽  
Study Results ◽  
Significant Difference ◽  
Egg Yolks ◽  
Lutein Content ◽  
Tbars Values

For the enrichment of eggs with lutein, a marigold flower extract – MFE (Tagetes erecta L.) was used. The two groups of laying hens were involved in the study – the control group (C) and the experimental group (E), respectively. The laying hens of the control group were fed by the lutein‐free mixture, while the laying hens’ E group consumed the mixture with the 3 g/kg of lutein. The laying hens’ feeding lasted for 31 days, after which the quality of eggs (i.e., the shape index, egg weight and the egg main parts, shell strength and thickness, Haugh units – HU, egg white height, egg yolk color, egg white pH, and the egg yolk pH), as well as the lutein content and Thiobarbituric Acid Reactive Substances (TBARS) values in egg yolks, were determined. The study results have demonstrated that the added MFE exerted an effect of thickness, weight, and eggshell proportion reduction (P&lt;0.001), as well as of the shell strength reduction (P=0.014). It has also reduced the HU value (P=0.039) and has increased the egg white content, egg yolk color, and the egg yolks’ lutein content (P&lt;0.001). A statistically significant difference in TBARS values was found between the fresh and the stored eggs in group C, as well as between the C and E groups in the fresh eggs (P&lt;0.05). Our results indicate that MFE is suitable for the enrichment of table eggs with lutein.

Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in laying Leghorn hens

2004 ◽  
Vol 142 (6) ◽  
pp. 683-690 ◽  
Author(s):  
C. H. CHENG ◽  
T. F. SHEN ◽  
W. L. CHEN ◽  
S. T. DING
Keyword(s):  
Transcription Factors ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Acid Deposition ◽  
Egg Production ◽  
Laying Hens ◽  
Regulatory Element ◽  
Egg Yolk ◽  
Total Fatty Acids ◽  
Egg Yolks

The purpose of the current study was to determine the effects of dietary docosahexaenoic acid (DHA) on fatty acid deposition in egg yolk and various tissues of laying Leghorn hens, and on the expression of hepatic lipogenesis-related transcription factors. There were three treatments with 10 Leghorn hens per treatment. Diets were based on maize-soybean meal (ME: 12 MJ/kg; CP: 171 g/kg; Ca: 34 g/kg) supplemented with 0 (plus 20 g butter/kg), 5 g (plus 15 g butter/kg) or 20 g algal DHA oil/kg. The egg production of the birds was not affected by dietary DHA oil (P>0·05). The DHA content in egg yolks of the laying hens increased significantly (P<0·01) with the dietary supplementation of DHA. The DHA content of the total fatty acids in the egg yolk of laying hens was 4±16, 11±43 and 20±90 g/kg for 0, 5 and 20 g/kg DHA treatments, respectively for the first week, and 6±16, 18±49 and 31±86 g/kg for the second week. Therefore, algal DHA oil can be utilized by laying Leghorn hens to enhance egg yolk DHA content and produce high DHA eggs. The concentrations of triacylglycerol (TG) and cholesterol in plasma of laying Leghorn hens were not affected by dietary DHA treatments (P>0·005). The DHA content in plasma and livers of laying hens was increased with the addition of DHA in the diet (P<0·05). The DHA content in the skeletal muscle of Leghorn hens was also increased with the increase in dietary DHA. The data indicate that dietary DHA oil can be incorporated into plasma, egg yolks and various tissues. The mRNA concentrations of the lipogenesis-related transcription factors, sterol regulatory element binding protein 1 (SREBP1) and SREBP2, in the livers of laying Leghorn hens were not affected by the supplement of algal DHA oil for 2 weeks, suggesting that the expressions of these transcription factors are tightly controlled and not sensitive to DHA treatments in laying hens.

scholarly journals Possibility of Using Astaxanthin-Rich Dried Cell Powder from Paracoccus carotinifaciens to Improve Egg Yolk Pigmentation of Laying Hens

Symmetry ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 923
Author(s):  
Masaki Honda ◽  
Yuki Kawashima ◽  
Kazuaki Hirasawa ◽  
Takeshi Uemura ◽  
Sun Jinkun ◽  
...  
Keyword(s):  
High Performance ◽  
Laying Hens ◽  
Egg Quality ◽  
Egg Yolk ◽  
Solvent System ◽  
Average Particle ◽  
Isomer Ratio ◽  
Quality Aspects ◽  
Egg Yolks ◽  
Feeding Diets

The study investigated egg quality aspects such as astaxanthin concentration, E/Z-isomer ratio, and yolk color in laying hens fed with astaxanthin-containing diets. Dried Paracoccus carotinifaciens cell powder (Panaferd-AX) and fine cell powder (Panaferd-P) were used as sources of astaxanthin, with average particle diameters of approximately 100 μm and 10 μm, respectively. Paracoccus carotinifaciens contains valuable rare carotenoids such as adonirubin and adonixanthin, and thus the concentrations of these carotenoids in egg yolk were also evaluated. The E/Z-isomer ratios of the egg yolk carotenoids were determined by normal-phase high-performance liquid chromatography (HPLC) with an improved solvent system. Feeding diets containing P. carotinifaciens resulted in increased concentrations of astaxanthin, adonirubin, and adonixanthin in egg yolk, as well as a marked increase in the yolk color fan score; values associated with the Panaferd-P-containing diet were higher than those associated with the Panaferd-AX-containing diet. For example, the astaxanthin concentration in egg yolks of hens fed with the Panaferd-AX- and Panaferd-P-containing diets for 21 days were 1.21 μg/g and 1.85 μg/g, respectively. This indicates that the pulverization treatment of the P. carotinifaciens powder increased the efficiency of carotenoid accumulation in the egg yolk. Moreover, more than 95% of astaxanthin in P. carotinifaciens was present as the all-E-isomer. However, approximately 25% of astaxanthin in egg yolk was present as the Z-isomers. In recent years, astaxanthin Z-isomers have attracted substantial attention as they exhibit a greater bioavailability and bioactivity than the all-E-isomer. These data are important not only for understanding egg yolk pigmentation but also for improving the nutritional value of hens’ egg yolk through the addition of P. carotinifaciens to their diet.

EFFECT OF AN ANTIBIOTIC ON LIPID FRACTIONS IN PLASMA AND EGG YOLK OF LAYING HENS

1963 ◽  
Vol 41 (10) ◽  
pp. 2171-2175
Author(s):  
P. J. Lupien ◽  
F. A. Vandenheuvel ◽  
W. G. Hunsaker ◽  
G. V. Hatina
Keyword(s):  
Egg Production ◽  
Species Differences ◽  
Laying Hens ◽  
Plasma Cholesterol ◽  
Egg Yolk ◽  
Human Beings ◽  
Cholesterol Levels ◽  
Lipid Fractions ◽  
Egg Yolks ◽  
Yolk Cholesterol

Supplementation of the basal ration of laying hens with 0.001, 0.005, and 0.01% neomycin sulphate did not produce any significant changes in the weight of the hens, their eggs, or egg yolks, nor did it alter egg production. Supplemental neomycin caused no significant changes in levels of plasma cholesterol. Plasma and yolk lipid levels varied considerably within as well as between groups of hens. Plasma squalene/plasma cholesterol and yolk squalene/yolk cholesterol ratios were approximately the same, indicating the presence of 250 times more cholesterol than squalene. Plasma squalene/yolk squalene and plasma cholesterol/yolk cholesterol ratios showed that both components were approximately 13 times more concentrated in the yolk than in the plasma. The variable plasma cholesterol levels for rats, rabbits, mice, human beings, as well as the laying hen, treated with neomycin, are suggestive of species differences in response.

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