Quantitative Campylobacter spp., antibiotic resistance genes, and veterinary antibiotics in surface and ground water following manure application: Influence of tile drainage control

2015 ◽  
Vol 532 ◽  
pp. 138-153 ◽  
Author(s):  
Steven K. Frey ◽  
Edward Topp ◽  
Izhar U.H. Khan ◽  
Bonnie R. Ball ◽  
Mark Edwards ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Ground Water ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Tile Drainage ◽  
Veterinary Antibiotics ◽  
Manure Application ◽  
Campylobacter Spp

Genotypic and Phenotypic Characterization of Salmonella Isolated from Fresh Ground Meats Obtained from Retail Grocery Stores in the Brookings, South Dakota, Area

2018 ◽  
Vol 81 (9) ◽  
pp. 1526-1534 ◽  
Author(s):  
ALAN K. ERICKSON ◽  
DEBRA L. MURRAY ◽  
LAURA A. RUESCH ◽  
MILTON THOMAS ◽  
ZACHARY LAU ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
South Dakota ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Genomic Sequences ◽  
Phenotypic Characterization ◽  
Grocery Stores ◽  
Ground Meat ◽  
Veterinary Antibiotics ◽  
Ground Pork

ABSTRACT Salmonella is one of the most common foodborne pathogens found in retail fresh meat products. The purpose of this study was to characterize the Salmonella that is found in common types of fresh ground meats available to consumers in grocery stores in the Brookings, South Dakota, area. Salmonella serotypes were detected in 50 (19%) of 261 retail fresh ground meat samples, with 2 (2%) of 115 ground turkey samples, 6 (14%) of 42 chicken samples, and 42 (40%) of 104 ground pork samples testing positive for Salmonella. The Salmonella isolates were sequenced using an Illumina MiSeq genome sequencer. The resulting genomic sequences were analyzed to determine the serotypes of the isolates and to detect the presence of virulence and antibiotic resistance genes. The Salmonella isolated from the ground meats belonged to 23 different serotypes. The predominant serotype isolated from ground chicken was Enteriditis (5 of 6, 83%). Among the ground pork isolates, the most common serotypes were the potential monophasic variant of Typhimurium (5 of 42, 12%), Uganda (5 of 42, 12%), Anatum (4 of 42, 10%), Derby (3 of 42, 7%), Infantis (3 of 42, 7%), and London (3 of 42, 7%). Among the 45 Salmonella isolates tested to determine their resistance to common veterinary antibiotics, 25 (56%) were found to be susceptible to all 14 antibiotics tested, 11 (24%) were resistant to 1 antibiotic, 4 (9%) were resistant to 2 antibiotics, 1 (2%) was resistant to 3 antibiotics, 2 (4%) were resistant to 4 antibiotics, 1 (2%) was resistant to 8 antibiotics, and 1 (2%) was resistant to 10 antibiotics. The most common antibiotic resistances observed in this study were to streptomycin (15 of 45, 33%), tetracycline (11 of 45, 24%), and sulfisoxazole (7 of 45, 16%). The results of phenotypic evaluation of antibiotic resistance profiles of Salmonella isolates correlated well with the antibiotic resistance genes detected in the genomic sequences of the isolates.

scholarly journals Detection and prevalence of antimicrobial resistance genes in Campylobacter spp. isolated from chickens and humans

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
Samantha Reddy ◽  
Oliver T. Zishiri
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Antibiotic Resistance Genes ◽  
Quinolone Resistance ◽  
Campylobacter Coli ◽  
Antimicrobial Resistance Genes ◽  
Campylobacter Spp ◽  
Clinical Cases

Campylobacter spp. are common pathogenic bacteria in both veterinary and human medicine. Infections caused by Campylobacter spp. are usually treated using antibiotics. However, the injudicious use of antibiotics has been proven to spearhead the emergence of antibiotic resistance. The purpose of this study was to detect the prevalence of antibiotic resistance genes in Campylobacter spp. isolated from chickens and human clinical cases in South Africa. One hundred and sixty one isolates of Campylobacter jejuni and Campylobacter coli were collected from chickens and human clinical cases and then screened for the presence of antimicrobial resistance genes. We observed a wide distribution of the tetO gene, which confers resistance to tetracycline. The gyrA genes that are responsible quinolone resistance were also detected. Finally, our study also detected the presence of the blaOXA-61, which is associated with ampicillin resistance. There was a higher (p < 0.05) prevalence of the studied antimicrobial resistance genes in chicken faeces compared with human clinical isolates. The tetO gene was the most prevalent gene detected, which was isolated at 64% and 68% from human and chicken isolates, respectively. The presence of gyrA genes was significantly (p < 0.05) associated with quinolone resistance. In conclusion, this study demonstrated the presence of gyrA (235 bp), gyrA (270 bp), blaOXA-61 and tetO antimicrobial resistance genes in C. jejuni and C. coli isolated from chickens and human clinical cases. This indicates that Campylobacter spp. have the potential of resistance to a number of antibiotic classes.

Impact of liquid hog manure applications on antibiotic resistance genes concentration in soil and drainage water in field crops

2020 ◽  
Vol 66 (10) ◽  
pp. 549-561
Author(s):  
Élodie Larouche ◽  
Mylène Généreux ◽  
Marie-Ève Tremblay ◽  
Mohamed Rhouma ◽  
Marc-Olivier Gasser ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Water Samples ◽  
Antibiotic Resistance Genes ◽  
Drainage Water ◽  
Manure Application ◽  
Tillage Practices ◽  
Hog Manure ◽  
The Impact ◽  
Soil And Water

Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteria in soil and drainage water under wheat and grain corn crops. Targeted ARGs tet(T), sul1, and blaCTX-M-1 in liquid hog manure, soil, and water samples were quantified by qPCR. Conventional PCR was used to detect mcr-1 and mcr-2. ARGs in control plots were detected despite the absence of manure, representing an environmental reservoir of resistant microorganisms. The manure application rate higher than 39 m3/ha increased tet(T) and sul1 gene concentrations in soil for more than 180 days. Tillage practices had no impact on ARG concentrations in soil and water samples. The blaCTX-M-1 gene was only detected in seven water samples in 2016, but no link was established with the treatments. The mcr-1 and mcr-2 genes were not detected in all tested samples. This study demonstrated that tet(T) and sul1 gene concentrations increased in soil after liquid hog manure application as well as in drainage water in the next weeks.

scholarly journals Manure Application Did Not Enrich Antibiotic Resistance Genes in Root Endophytic Bacterial Microbiota of Cherry Radish Plants

2019 ◽  
Vol 86 (2) ◽  
Author(s):  
Yu-Jing Zhang ◽  
Hang-Wei Hu ◽  
Qing-Lin Chen ◽  
Hui Yan ◽  
Jun-Tao Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Structural Equation ◽  
Organic Fertilizer ◽  
Antibiotic Resistance Genes ◽  
Pot Experiment ◽  
Equation Modeling ◽  
Manure Application ◽  
Edible Part ◽  
Bacterial Microbiota

ABSTRACT Growing evidence suggests that livestock manure used as organic fertilizer in agriculture may lead to the potential propagation of antibiotic resistance genes (ARGs) from “farm to fork.” However, little is known about the impacts of manure fertilization on the incidence of ARGs in the plant-associated microbiomes (including rhizosphere, endosphere, and phyllosphere), which hampers our ability to assess the dissemination of antibiotic resistance in the soil-plant system. Here, we constructed a pot experiment to explore the effects of poultry and cattle manure applications on the shifts in the resistome in the plant microbiome of harvested cherry radish. A total of 144 ARGs conferring resistance to eight major classes of antibiotics were detected among all the samples. Rhizosphere and phyllosphere microbiomes harbored significantly higher diversity and abundance of ARGs than did root endophytic microbiomes of cherry radish. Manure application significantly increased the abundance of ARGs in the rhizosphere and phyllosphere but not in the endophytes of the root, which is the edible part of cherry radish. Soil and plant microbiomes changed dramatically after manure applications and clustered separately according to different sample types and treatments. Structural equation modeling revealed that bacterial abundance was the most important factor modulating the distribution patterns of soil and plant resistomes after accounting for multiple drivers. Taken together, we provide evidence that enrichment of the resistome in the rhizosphere and phyllosphere of cherry radish is more obvious than with the endosphere after manure application, suggesting that manure amendment might not enhance the dissemination of ARGs into the root of vegetables in the pot experiment. IMPORTANCE Our study provides important evidence that manure application increased the occurrence of ARGs in the rhizosphere and phyllosphere of cherry radish, compared with that in the endophytic bacterial microbiota of root, which is the edible part of cherry radish. Our findings suggest that although manure amendment is a significant route of ARGs entering agricultural soils, these manure-derived ARGs may be at low risk of migrating into the endophytes of root vegetables.

Effect of Manure Application on Abundance of Antibiotic Resistance Genes and Their Attenuation Rates in Soil: Field-Scale Mass Balance Approach

2014 ◽  
Vol 48 (5) ◽  
pp. 2643-2650 ◽  
Author(s):  
Nicole Fahrenfeld ◽  
Katharine Knowlton ◽  
Leigh Anne Krometis ◽  
W. Cully Hession ◽  
Kang Xia ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Mass Balance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Field Scale ◽  
Manure Application ◽  
Balance Approach ◽  
Mass Balance Approach

scholarly journals Monitoring Antibiotic Residues and Corresponding Antibiotic Resistance Genes in an Agroecosystem

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Yasser M. Awad ◽  
Kwon Rae Kim ◽  
Sung-Chul Kim ◽  
Kangjoo Kim ◽  
Sang Ryong Lee ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
High Performance ◽  
Solid Phase ◽  
Antibiotic Resistance Genes ◽  
Rice Paddy ◽  
Veterinary Antibiotics ◽  
Antibiotic Overuse ◽  
Sediment Samples ◽  
Qualitative Polymerase Chain Reaction

Antibiotic resistance genes (ARGs) have been commonly reported due to the overuse worldwide of antibiotics. Antibiotic overuse disturbs the environment and threatens public human health. The objective of this study was to measure the residual concentrations of veterinary antibiotics in the tetracycline group (TCs), including tetracycline (TC) and chlortetracycline (CTC), as well as those in the sulfonamide group (SAs), including sulfamethazine (SMT), sulfamethoxazole (SMX), and sulfathiazole (STZ). We also isolated the corresponding ARGs in the agroecosystem. Four sediment samples and two rice paddy soil samples were collected from sites near a swine composting facility along the Naerincheon River in Hongcheon, Korea. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed with a solid-phase extraction method to measure the concentration of each antibiotic. ARGs were identified by the qualitative polymerase chain-reaction using synthetic primers. SAs and their corresponding ARGs were highly detected in sediment samples whereas TCs were not detected except for sediments sample #1. ARGs for TCs and SAs were detected in rice paddy soils, while ARGs for TCs were only found in sediment #2 and #4. Continuous monitoring of antibiotic residue and its comprehensive impact on the environment is needed to ensure environmental health.

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