Extraction method development for nanoplastics from oyster and fish tissues

2021 ◽  
pp. 152675
Author(s):  
Yu-Shan Chang ◽  
Shih-Hsuan Chou ◽  
Ya-Jhu Jhang ◽  
Tai-Sing Wu ◽  
Li-Xin Lin ◽  
...  
Keyword(s):  
Extraction Method ◽  
Method Development ◽  
Fish Tissues

scholarly journals Extraction of Honey Polyphenols: Method Development and Evidence of Cis Isomerization ubertas Academica

2016 ◽  
Vol 11 ◽  
pp. ACI.S39739 ◽  
Author(s):  
Thibaut Istasse ◽  
Nicolas Jacquet ◽  
Thomas Berchem ◽  
Eric Haubruge ◽  
Bach Kim Nguyen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Acids ◽  
Extraction Method ◽  
High Performance ◽  
Method Development ◽  
Solid Phase ◽  
Phase Extraction ◽  
Minor Components ◽  
Coumaric Acid ◽  
Wild Carrot

Honey polyphenols have been studied with the objective of relating honeys to their floral sources. Initially synthesized by plant, these polyphenols can be found in the plant's nectar, which are collected by bees, which convert the nectar into honey. Consequently, polyphenols constitute minor components of honey. The development of a solid-phase extraction method for honey polyphenols is presented in this study. The technique employs Amberlite XAD-2 adsorbent and was tested on monofloral honeys from six different plants: acacia, chestnut, eucalyptus, thyme, sunflower, and wild carrot. Analyses were performed using high-performance liquid chromatography coupled with UV detection and mass spectrometry. Several phenolic acids and flavonoids were identified: caffeic and p-coumaric acids, quercetin, kaempferol, naringenin, chrysin, and pinocembrin. Generally, the quantity of a given polyphenol in the honey was around 0.2 mg/100 g of honey, except for chestnut honey, which contained around 3.0 mg of p-coumaric acid/100 g of honey. Analyses highlighted significant formation of cis isomers for phenolic acids during the extraction despite protection from light.

scholarly journals Bio-Analytical Method Development of Repaglinide Drug Delivery Systems

2019 ◽  
Vol 9 (6) ◽  
pp. 140-142
Author(s):  
B. Ramu ◽  
Kaushal K Chandrul ◽  
P. Shanmuga Pandiyan
Keyword(s):  
Phosphate Buffer ◽  
Extraction Method ◽  
Mobile Phase ◽  
High Performance ◽  
Drug Delivery Systems ◽  
Method Development ◽  
Extraction Efficiency ◽  
Ultraviolet Spectroscopy ◽  
Rabbit Plasma ◽  
Solvent Extraction Method

A sensitive, specific and rapid high-performance liquid chromatography-ultraviolet spectroscopy method was developed and successfully validated to estimate the repaglinide in rabbit plasma. The solvent extraction method was used for  repaglinide from serum by using ethyl acetate and 0.1N HCl. The mobile phase consists of acetonitrile: phosphate buffer pH 4.0 at 60:40 %v/v with 1% triethylamine at flow rate of 0.8ml/min and at fixed wavelength of 254nm. On ten minutes of run time, repaglinide was retention at 7.4min. The extraction efficiency 95% for repaglinide. The intra-day and inter-day precision was in the terms of %RSD less than 1.76%. The developed method was validated and proposed method is useful for pharmacokinetics studies.  Keywords: Anti-diabetics, HPLC,Methanol,Phosphate buffer, Repaglinide

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

High throughput semi-automated extraction method for the creation of a collection from microbial fermentations of fungi and Actinomycetes

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
JR Tormo ◽  
N Tabanera ◽  
D Conway ◽  
P Ramos ◽  
A Redondo ◽  
...  
Keyword(s):  
High Throughput ◽  
Extraction Method ◽  
Automated Extraction ◽  
The Creation
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