Comprehensive bioanalytical multi-imaging by planar chromatography in situ combined with biological and biochemical assays highlights bioactive fatty acids in abelmosk

Talanta ◽  
2021 ◽  
Vol 223 ◽  
pp. 121701
Author(s):  
N.G.A.S. Sumudu Chandana ◽  
Gertrud E. Morlock
Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
E Rozema ◽  
N Fakhrudin ◽  
A Atanasov ◽  
D Schuster ◽  
E Heiss ◽  
...  

2021 ◽  
pp. 1-38
Author(s):  
B. Mohtashami ◽  
H. Khalilvandi-Behroozyar ◽  
R. Pirmohammadi ◽  
M. Dehghan-Banadaky ◽  
M. Kazemi-Bonchenari ◽  
...  

Abstract This study aimed to evaluate the effects of different supplemental fat sources [soybean oil (SBO) as a source of n-6 fatty acid and fish oil (FO) as a source of n-3 fatty acids] in the starter feed of milk-fed dairy calves during the hot season. Forty Holstein calves (3 d of age; 39.67 kg of body weight; ten calves per group) were randomly assigned to the experimental treatments as follows: (1) starter feed supplemented with no fat source (CON), (2) starter feed supplemented with 3% SBO (DM basis), (3) starter feed supplemented with 3% FO (DM basis), and (4) starter feed supplemented with an equal mixture of SBO and FO (1.5% each, DM basis). The milk feeding schedule was constant for treatments and all calves were weaned on d 65 of age. Results show that calves had greater starter intake, average daily gain, and body length when fed SBO compared to other treatments. However, feed efficiency was increased and inflammatory indicators (tumor necrosis factor-alpha, serum amyloid A and haptoglobin) concentrations were reduced in the calves fed FO compared to the other treatments. In summary, it was revealed that SBO rich in n-6 FA improved starter intake and growth performance, while FO rich in n-3 FA could improve the immune function of calves. Due to the current experimental condition, an equal mixture of SBO and FO (1.5% each, DM basis) can be recommended to have an optimum growth performance and immune function while the calves are reared under the heat conditions.


2021 ◽  
pp. 126430
Author(s):  
R.J. Jones ◽  
J. Massanet-Nicolau ◽  
R. Fernandez-Feito ◽  
R.M. Dinsdale ◽  
A.J. Guwy

2014 ◽  
Vol 16 (3) ◽  
pp. 1507 ◽  
Author(s):  
Derek R. Vardon ◽  
Brajendra K. Sharma ◽  
Humberto Jaramillo ◽  
Dongwook Kim ◽  
Jong Kwon Choe ◽  
...  

2006 ◽  
Vol 55 (4) ◽  
pp. 261-271 ◽  
Author(s):  
Fowad Akraim ◽  
Marie-Claude Nicot ◽  
Pierre Weill ◽  
Francis Enjalbert

2021 ◽  
Author(s):  
Xiao-Qing Hou ◽  
Dan-Dan Zhang ◽  
Daniel Powell ◽  
Hong-Lei Wang ◽  
Martin N. Andersson ◽  
...  

In insects, airborne chemical signals are mainly detected by two receptor families, odorant receptors (ORs) and ionotropic receptors (IRs). Functions of ORs have been intensively investigated in Diptera and Lepidoptera, while the functions and evolution of the more ancient IR family remain largely unexplored beyond Diptera. Here, we identified a repertoire of 26 IRs from transcriptomes of female and male antennae, and ovipositors in the moth Agrotis segetum. We observed that a large clade formed by IR75p and IR75q expansions is closely related to the acid-sensing IRs identified in Diptera. We functionally assayed each of the five AsegIRs from this clade using Xenopus oocytes and found that two receptors responded to the tested ligands. AsegIR75p.1 responded to several compounds but hexanoic acid was revealed to be the primary ligand, and AsegIR75q.2 responded primarily to octanoic acid, and less so to nonanoic acid. It has been reported that the C6-C10 medium-chain fatty acids repel various insects including many drosophilids and mosquitos. Our GC-EAD recordings showed that C6-C10 medium-chain fatty acids elicited antennal responses of both sexes of A. segetum, while only octanoic acid had repellent effect to the moths in a behavioural assay. In addition, using fluorescence in situ hybridization, we demonstrated that AsegIR75q.2 and its co-receptor AsegIR8a are not located in coeloconic sensilla as found in Drosophila, but in basiconic or trichoid sensilla. These functional data in combination with our phylogenetic analysis suggest that subfunctionalization of the acid-sensing IRs after gene duplication plays an important role in the evolution of ligand specificities of the acid-sensing IRs in Lepidoptera.


2014 ◽  
Vol 11 (14) ◽  
pp. 3729-3738 ◽  
Author(s):  
K. E. Larkin ◽  
A. J. Gooday ◽  
C. Woulds ◽  
R. M. Jeffreys ◽  
M. Schwartz ◽  
...  

Abstract. Foraminifera are an important component of benthic communities in oxygen-depleted settings, where they potentially play a significant role in the processing of organic matter. We tracked the uptake of a 13C-labelled algal food source into individual fatty acids in the benthic foraminiferal species Uvigerina ex. gr. semiornata from the Arabian Sea oxygen minimum zone (OMZ). The tracer experiments were conducted on the Pakistan margin during the late/post monsoon period (August–October 2003). A monoculture of the diatom Thalassiosira weisflogii was 13C-labelled and used to simulate a pulse of phytoplankton in two complementary experiments. A lander system was used for in situ incubations at 140 m water depth and for 2.5 days in duration. Shipboard laboratory incubations of cores collected at 140 m incorporated an oxystat system to maintain ambient dissolved oxygen concentrations and were terminated after 5 days. Uptake of diatoms was rapid, with a high incorporation of diatom fatty acids into foraminifera after ~ 2 days in both experiments. Ingestion of the diatom food source was indicated by the increase over time in the quantity of diatom biomarker fatty acids in the foraminifera and by the high percentage of 13C in many of the fatty acids present at the endpoint of both in situ and laboratory-based experiments. These results indicate that


1959 ◽  
Vol 196 (6) ◽  
pp. 1238-1241 ◽  
Author(s):  
Y. Stein ◽  
B. Shapiro

Up to 60% of i.v. injected doses of 1-C14 palmitic acid was found in the liver 15 minutes after injection, two thirds in the form of triglycerides and the rest as phospholipids. Almost no radioactive UFA could be demonstrated in the liver or in blood plasma. Thereafter, the activity in the triglyceride fraction fell rapidly, only 50% remaining after 1 hour. At the same time the phospholipid activity increased. With 1-C14 linoleic acid a similar pattern was found, except that the percentage retained in the liver was lower and maximum retention was found after 5 minutes. Fifteen minutes after injection of 1-C14 palmitate most of the radioactive glycerides in the liver were found in the microsomes and mitochondria, with very little in the floating fat. Equilibration between these cytoplasmatic particles occurred only after 2 hours. Perfusion of the liver in situ with blood or blood substitutes, after various periods following 1-C14 palmitic acid injection, caused a release of about 5–20% of the liver triglycerides and 0.5–2% of the liver phospholipids per hour.


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