The value of trans-scrotal ultrasonography at bull breeding soundness evaluation (BBSE): The relationship between testicular parenchymal pixel intensity and semen quality

Oxidative stress (OS) is involved in many disoders including male infertility. Human spermatozoa are very sensitive targets of reactive oxygen species (ROS) and most sperm functions are impaired in the case of OS. In addition unbalanced production of ROS is considered one of the most important causes of sperm DNA fragmentation, a semen trait of infertile men. The relationship between oxidative damage and semen quality is partially controversial, probably due to the different methods and/or targets used to reveal the OS. In this study, by fluorescence microscopy and flow cytometry, we compared two methods to reveal 8-hydroxy,2-deoxyguanosine (8-OHdG), the hallmark of oxidative DNA damage: an immunofluorescence method and the commercial OxyDNA kit. We found that although both methods localized the labelling in sperm nuclei they yielded different measures, and only with the immunofluorescence method was the labelling specific for sperm 8-OHdG. The immunofluorescence method, coupled to flow cytometry, was thus selected to analyse the 8-OHdG content in semen samples from 94 subfertile patients and to investigate the relationship with semen quality. We found that the percentages of spermatozoa with 8-OHdG (mean±s.d., 11.4±6.9%) were related to sperm count (Pearson's correlation coefficient (r)=−0.27, P=0.04 (ANOVA and student's t-test)), motility (progressive: r=−0.22, P=0.04; non-progressive: r=0.25, P=0.01), and normal morphology (r=−0.27, P=0.01). In conclusion, we demonstrate that immunofluorescence/flow cytometry is a reliable and specific method to detect 8-OHdG at single-cell level and show that oxidative damage only partially overlaps poor semen quality, suggesting that it could provide additional information on male fertility with respect to routine semen analysis.

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Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

Journal of Feline Medicine and Surgery ◽

10.1177/1098612x19889072 ◽

2019 ◽

Vol 22 (10) ◽

pp. 882-889 ◽

Cited By ~ 1

Author(s):

María F García ◽

Romina Nuñez Favre ◽

María C Stornelli ◽

Ramiro Rearte ◽

María C García Mitacek ◽

...

Keyword(s):

Seminal Plasma ◽

Sperm Morphology ◽

Semen Quality ◽

Sperm Count ◽

Sodium Dodecyl ◽

Sperm Concentration ◽

Membrane Integrity ◽

Domestic Cat ◽

Sds Page ◽

The Relationship

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

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The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch

Theriogenology ◽

10.1016/0093-691x(93)90397-n ◽

1993 ◽

Vol 39 (2) ◽

pp. 553-560 ◽

Cited By ~ 25

Author(s):

W.D. Mickelsen ◽

M.A. Memon ◽

P.B. Anderson ◽

D.A. Freeman

Keyword(s):

Pregnancy Rate ◽

Litter Size ◽

Artificial Insemination ◽

Semen Quality ◽

Relationship Of ◽

The Relationship

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The Influence of Varicocele and Microsurgical Varicocelectomy on Semen Quality, as Determined by Metabolic Analysis

10.21203/rs.3.rs-663322/v1 ◽

2021 ◽

Author(s):

Xinzong Zhang ◽

Cuncan Deng ◽

Wujiang Liu ◽

Huang Liu ◽

Yu Zhou ◽

...

Keyword(s):

Western Blotting ◽

Seminal Plasma ◽

Semen Quality ◽

Reproductive Function ◽

Normal Subjects ◽

Metabolic Abnormality ◽

Human Tissues ◽

Metabolic Analysis ◽

The Relationship

Abstract The influence of varicocele and microsurgical varicocelectomy on semen quality remains unclear. No previous study has investigated the relationship between semen metabolism and the abnormalities in reproductive function caused by varicocele. Here, we used the non-targeted and targeted metabolic analysis to investigate the different metabolites in seminal plasma within normal, varicocele, and varicocelectomy groups. We clearly showed that varicocele significantly affects sperm metabolism, and microsurgical varicocelectomy can reverse this metabolic abnormality. Moreover, we characterized the landscape of three dipeptides in the seminal plasma of patients with varicocele that have not been identified previously in human tissues or biofluids. Interestingly, the levels of these three dipeptides decreased after microsurgical varicocelectomy coincident with an improvement in semen quality. Western blotting confirmed the downregulation of DPEP3 (dipeptidase 3) in the varicocele group and the upregulation of DPEP3 in the varicocelectomy group. Furthermore, we found that eight metabolites may be helpful to distinguish varicocele patients from normal subjects. Our results can be applied to earlier diagnosis or to predict the outcome of microsurgery for varicocele.

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TYROSINE KINASE GENE POLYMORPHISMS ASSOCIATE WITH FRESH SEMEN QUALITY FROM DAIRY BULL FRIESIAN HOLSTEIN

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v52i4.1416 ◽

2021 ◽

Vol 52 (4) ◽

pp. 1050-1057

Author(s):

Hernawati & et al.

Keyword(s):

Semen Quality ◽

Dna Amplification ◽

Kinase Gene ◽

Significance Level ◽

Tyrosine Kinase Gene ◽

Pcr Method ◽

Dairy Bull ◽

The Relationship ◽

Fresh Semen

This research aims to identify polymorphisms in TEK genes to identify any related possibility to fresh semen quality of FH bull using the PCR method. A total of 14 samples of bull’s whole blood were collected and also the quality of each bull’s fresh semen. DNA amplification was carried out using primer Forward (TEK_F) 5'-TAGATTGTCGCTTGCCTGGG-3 'and Reverse (TEK_R) 5'-CCTGTGCCGACAGGTTTACT-3'. Analysis of the DNA sequence results was carried out using BioEdit and NCBI BLAST software. The results showed that of the 7 samples producing 262 bp and found polymorphisms in the TEK gene sequence in 23 gene bank databases. In the analysis of the relationship between the motility of individual spermatozoa with mutations, r count> r table (0.806> 0.754) or significance value <5% significance level (0.029 <0.050). In the analysis of the relationship between semen concentration and mutation, r count> r table (0.897> 0.754) or significance level <5% significance level (0.006 <0.050) is obtained.

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Association Between Meat Intake and Semen Parameters Among Iranian Infertile Men: A Cross-sectional Study

10.21203/rs.3.rs-29618/v1 ◽

2020 ◽

Author(s):

Reza Ghiasvand ◽

farahnaz haeri ◽

SeyyedPayam ShariatPanahi ◽

Laleh Dehghan Marvast

Keyword(s):

Semen Quality ◽

Cross Sectional Study ◽

Semen Parameters ◽

Sectional Study ◽

Cross Sectional ◽

Meat Intake ◽

Canned Tuna ◽

Infertile Men ◽

The Relationship ◽

Serving Size

Abstract Objective: Previous studies have proven the effect of dietary patterns on semen quality indicators, but research on the relationship between meat intake and semen quality is limited. Therefore, this study was conducted to investigate the relationship between Meat intake with semen quality.Methods: In this cross sectional study, 400 infertile men were recruited into study during their fertility investigation in Yazd Reproductive Sciences Institute Diagnosed by an andrologist according to the inclusion criteria. Multivariate logistic regression was used to determine the relationship between meat intake and semen parameters. All data were analyzed using SPSS V. 22 software.Results: We found that intake of canned tuna can have two different effects on sperm motility in infertile male. Intake of canned tuna according to the serving size stated in quartile 2, leads to a decrease in the percentage of immotile sperm from 52.93 (CI95%, 51.15-54.71) to 46.55 (CI95%, 44.56-48.54)(Ptrend=0.036). On the other hand, there is an increase in the percentage of immotile sperm from 46.55 (CI95%, 44.56-48.54) to 52.88 (CI95%, 50.94-54.82) in the highest quartile of canned tuna intake.Also, no significant relationship was observed between intake other types of meats and sperm quality indices.Conclusions: We found that intake of canned tuna,base on serving size of quartile 2, is associated with lower percentage of immotile sperm, on the other hand high intake of canned tuna increase percentage of immotile sperm in Iranian infertile men. More extensive studies are recommended in this regard.

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Reproductive and endocrinological effects of Benign Prostatic Hyperplasia and finasteride therapy in dogs

Scientific Reports ◽

10.1038/s41598-020-71691-7 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Daniel S. R. Angrimani ◽

Maíra M. Brito ◽

Bruno R. Rui ◽

Marcílio Nichi ◽

Camila I. Vannucchi

Keyword(s):

Benign Prostatic Hyperplasia ◽

Semen Quality ◽

Clinical Signs ◽

Negative Influence ◽

Prostatic Hyperplasia ◽

Reproductive Disorders ◽

Short Term ◽

Prostatic Fluid ◽

Therapeutic Measures ◽

Breeding Soundness

Abstract Benign prostatic hyperplasia (BPH) is one of the most important reproductive disorders in aging dogs. Therapeutic measures include orchiectomy and pharmacological treatment, leading to reduction of prostate volume and clinical signs. One of the most common drugs used in BPH treatment is finasteride, but data regarding its possible side effects are scarce. Thus, the aim of this study was to evaluate the effects of BPH and short-term (2 months) finasteride therapy on clinical, endocrinological, and reproductive parameters in dogs. Dogs were allocated into four experimental groups: Non-affected (n = 5), BPH (n = 5), Non-Affected-Finasteride (n = 5) and BPH-Finasteride (n = 5) groups. Dogs were evaluated monthly during 2 months by a complete breeding soundness examination, B-mode ultrasound and Doppler ultrasonography of the testicular artery, hormonal profile (testosterone, estrogen and dihydrotestosterone) and oxidative profile of the prostatic fluid. After 2 months, dogs were gonadectomized and testicles were subjected to histologic analysis. Finasteride treatment reduced dihydrotestosterone concentrations, without negative influence on semen quality and also reverted testicular hemodynamics changes of BPH. On the other hand, BPH was accompanied by significant changes in testosterone and estrogen concentrations and semen quality, mainly related to sperm kinetics alterations. In conclusion, BPH dogs have important hormonal and sperm alterations, however, short-term finasteride treatment (2 months) was able to reduce overall effects of BPH, thus representing a method of therapy for BPH treatment.

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Urospermia indicating ectopic ureters in breeding dogs – 3 cases

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/a-0862-5443 ◽

2019 ◽

Vol 47 (02) ◽

pp. 119-124

Author(s):

Anne-Rose Günzel-Apel ◽

Karola Wolf ◽

Franziska Werhahn Beining ◽

Anke Baumeister ◽

Jan-Peter Bach ◽

...

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Sperm Count ◽

Labrador Retriever ◽

Semen Collection ◽

Ectopic Ureters ◽

Breeding Soundness

AbstractAn Entlebucher Mountain Dog (57 months old, case 1), a Labrador Retriever (24 months, case 2) and an Irish Soft-Coated Wheaten Terrier (31 months old, case 3) were presented for breeding soundness evaluation to the clinic. During semen collection in all 3 dogs, the pre-secretion and the sperm-rich fraction showed normal consistency and colour, whereas the prostatic secretion (3 rd ejaculate fraction) appeared strikingly yellow. In cases 1 and 2, a severely decreased sperm motility (asthenozoospermia) and an increased amount of abnormal spermatozoa (teratozoospermia), and in case 3, a moderately decreased total sperm count (oligozoospermia) were detected. Sonographical examination revealed abnormal findings regarding the uretero-vesical junction and ectopic ureters. Therefore it is concluded that urine admixture to the 3 rd ejaculate fraction may indicate the presence of ectopic ureters and may cause impairment of semen quality and fertility. The present cases raise questions regarding urospermia concerning: 1. its incidence in dogs in general and in connection with ectopic ureters and 2. its relevance as a cause of deficient ejaculate quality and subfertility or infertility.

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PSIII-11 Semen quality effect on conception rate among three purebred swine lines

Journal of Animal Science ◽

10.1093/jas/skz122.288 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 163-163

Author(s):

Giselle Kruse ◽

Cailtyn Bruns

Keyword(s):

Logistic Regression ◽

Random Effects ◽

Odds Ratio ◽

Fixed Effects ◽

Logistic Regression Model ◽

Semen Quality ◽

Conception Rate ◽

Conception Rates ◽

The Relationship ◽

Effect Of Age

Abstract The objective of this study was to examine the relationship between semen quality and conception rate. There were 30985 Duroc, 15888 Landrace, and 18198 Yorkshire single sire, purebred mating observations with 880 Duroc, 508 Landrace, and 492 Yorkshire boars represented from March 2011 to July 2018. Data from each breed was analyzed using a logistic regression model with fixed effects of sow parity at time of mating, the number of breeding attempts for the sow, days rest of the boar, and days from collection to insemination, covariates of age at collection, motility, percent abnormal tails, and random effects of contemporary group, boar, and evaluator. For Duroc, increased percent abnormal tails decreased conception rate (odds ratio=0.99, P < 0.05), and more than 9 days rest resulted in lower conception rates compared to less than 5 days rest (odds ratio=0.85, P < 0.05). For all three breeds, inseminations occurring 2 days post-collection tended to result in lower conception rate compared to 1 day post-collection (Duroc, odds ratio=0.94, P < 0.1, Yorkshire, odds ratio=0.87, P < 0.1, Landrace, odds ratio=0.91,P < 0.05); however, for Landrace, inseminations occurring 4 days post collection resulted in higher conception rates compared to 1 day post-collection (odds ratio=1.16, P < 0.05). For Yorkshire, increased motility was associated with higher conception rates (odds ratio=1.02, P < 0.001). The effect of age at collection on conception rate was small, but significant for both Landrace and Duroc resulting in ~1% increase per year of age (P < 0.05). Boar and semen quality effects on conception rate were inconsistent across breeds, suggesting that optimizing conception rates across breeds could be difficult.

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